Epsilon Class Glutathione S-Transferase Confers Phoxim Tolerance in the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae).

The black cutworm Agrotis ipsilon is a serious crop pest. Phoxim, an organophosphorus insecticide, has been widely used to control A. ipsilon. When phoxim is extensively applied, the susceptibility of A. ipsilon to insecticide is reduced. However, the mechanisms of tolerance of A. ipsilon to phoxim remain unclear. Herein, we report that an epsilon class glutathione S-transferase, AiGSTE1, confers phoxim tolerance in A. ipsilon. Exposure to a sublethal concentration (LC50) of phoxim caused oxidative stress and activated the transcription of AiGSTe1 genes in A. ipsilon larvae. Recombinant AiGSTE1 expressed in Escherichia coli could metabolize phoxim. Furthermore, E. coli cells overexpressing AiGSTE1 displayed significant tolerance to oxidative stress. Knockdown of AiGSTe1 by RNA interference significantly increased the mortality of A. ipsilon larvae to phoxim. These results demonstrate that AiGSTE1 confers phoxim tolerance in A. ipsilon by metabolizing the insecticide and preventing phoxim-induced oxidative stress.

Identification of 14 glutathione S-transferase genes from Lasioderma serricorne and characterization of LsGSTe1 involved in lambda-cyhalothrin detoxification.

In insects, glutathione S-transferases (GSTs) play a pivotal role in the detoxification of a wide range of pesticides. The cigarette beetle, Lasioderma serricorne, is an economically important pest insect of stored products. Recently, pyrethroid insecticides have been used to control this pest. However, little is known concerning the responses and functions of GSTs in L. serricorne under pyrethroid exposure. In this study, transcriptome sequencing was performed on L. serricorne, and a total of 14 GSTs were identified by retrieving the unigene dataset. Of these, 13 predicted GSTs fell into six cytosolic classes, namely, delta, epsilon, omega, sigma, theta, and zeta, and one was assigned to an "unclassified" group. The GST genes were differentially expressed in various larval tissues and at different developmental stages. Exposure to the pyrethroid insecticide lambda-cyhalothrin (LCT) caused oxidative stress in L. serricorne larvae and led to significantly elevated expression levels of six genes, among which LsGSTe1 was the most upregulated. Recombinant LsGSTE1 protein displayed LCT-metabolizing activity. Furthermore, LsGSTE1 protects cells against oxidative stress. Moreover, knockdown of LsGSTe1 by RNA interference dramatically increased the susceptibility of L. serricorne larvae to LCT treatment. The results from this study provide sequence resources and expression data for GST genes in L. serricorne. Our findings indicate that LsGSTE1 plays a dual role in LCT detoxification by metabolizing the pesticide and by preventing LCT-induced oxidative stress. Thus, the LsGSTe1 gene could be used as a potential target for sustainable management of the cigarette beetle.

Macrophage polarization states in atherosclerosis.

Atherosclerosis, a chronic inflammatory condition primarily affecting large and medium arteries, is the main cause of cardiovascular diseases. Macrophages are key mediators of inflammatory responses. They are involved in all stages of atherosclerosis development and progression, from plaque formation to transition into vulnerable plaques, and are considered important therapeutic targets. Increasing evidence suggests that the modulation of macrophage polarization can effectively control the progression of atherosclerosis. Herein, we explore the role of macrophage polarization in the progression of atherosclerosis and summarize emerging therapies for the regulation of macrophage polarization. Thus, the aim is to inspire new avenues of research in disease mechanisms and clinical prevention and treatment of atherosclerosis.

A revision of the geographical distributions of the shrews Crocidura tanakae and C. attenuata based on genetic species identification in the mainland of China.

The Taiwanese gray shrew (Crocidura tanakae) and Asian gray shrew (C. attenuata) are so similar in size and morphology that the taxonomic status of the former has changed several times since its description; C. tanakae has also been regarded as an endemic species of Taiwan Island. In recent years, molecular identification has led to several reports of C. tanakae being distributed in the mainland of China. In this study, we determine the geographical distribution of C. attenuata and C. tanakae based on more than one hundred specimens collected during 2000 to 2018 over a wide area covering the traditional ranges of the two species in the mainland of China, and show a substantial revision of their distributions. Among 110 individuals, 33 C. attenuata and 77 C. tanakae were identified by Cytb gene and morphologies. Our results show, (1) C. attenuata and C. tanakae are distributed sympatrically in the mainland of China; (2) contrary to the previous reports, the distribution range of C. attenuata is restricted and much smaller than that of C. tanakae in the mainland of China; (3) Hainan Island, like Taiwan Island, is inhabited by C. tanakae only according to the present data.

Application of the analytic hierarchy process to a sustainability assessment of coastal beach exploitation: a case study of the wind power projects on the coastal beaches of Yancheng, China.

Sustainability assessments of coastal beach exploitation are difficult because the identification of appropriate monitoring methodologies and evaluation procedures is still ongoing. In particular, the most suitable procedure for the application of sustainability assessment to coastal beaches remains uncertain. This paper presents a complete sustainability assessment process for coastal beach exploitation based on the analytic hierarchy process (AHP). We developed an assessment framework consisting of 14 indicators derived from the three dimensions of suitability, economic and social value, and ecosystem. We chose a wind power project on a coastal beach of Yancheng as a case study. The results indicated that the wind power farms on the coastal beach were not completely in keeping with sustainable development theory. The construction of the wind power farms had some negative impacts. Therefore, in the design stage, wind turbines should be designed and planned carefully to minimize these negative impacts. In addition, the case study demonstrated that the AHP was capable of addressing the complexities associated with the sustainability of coastal beaches.

Occurrence and removal of polycyclic aromatic hydrocarbons in the wastewater treatment process.

The occurrence and removal of polycyclic aromatic hydrocarbons (PAHs) was examined at different stages at a modified anaerobic-anoxic-oxic biological wastewater treatment plant in Tai'an City in eastern China. Twelve PAHs were detectable, with low molecular weight PAHs found at different treatment stages in wastewater and sludge. The most frequent and abundant PAHs in the influents were different in May and November. In May, 3- and 4-ringed PAH compounds were predominant, accounting for 53.5% of the ∑PAHs. However, in November, 2-ringed PAH compounds were most frequent and abundant, accounting for 90.1% of the ∑PAHs. In May and November, the removal efficiencies of individual PAH compounds ranged from 72.4 to 99.4% and from 21.1% to 100%, respectively, in the total treatment process. A significant relationship was observed between the removal efficiency and log K(ow) of the PAH compounds in the grit stage (when the values of log K(ow) were higher than approximately 5), suggesting that these compounds were principally removed through sorption to sludge particles and transfer to sludge processing systems. The residence time impacted the removal efficiency in the biological treatment process at the primary and secondary stages. In addition, the removal efficiencies depended dramatically on seasonal variations.