Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 16 de 16
Filter
Add more filters










Publication year range
2.
Sci Total Environ ; 915: 170115, 2024 Mar 10.
Article in English | MEDLINE | ID: mdl-38232848

ABSTRACT

Grafting is an effective horticultural method to reduce Cd accumulation in crops. However, the mechanism of grafting inducing the decrease in Cd content in scions remains unclear. This study evaluated the effect of grafting on fruit quality, yield, and Cd content of Solanum melongena, and explored the potential mechanism of grafting reducing Cd content in scions. In the low Cd-contaminated soil, compared with un-grafted (UG) and self-grafted plants (SG), the fruit yield of inter-grafted plants (EG) increased by 38 %, and the fruit quality was not markedly affected. In EG, the decrease in total S and Cd content was not related to organic acids and thiol compounds. The decrease in total S and Cd content in EG leaves and fruits was closely related to the synthesis and transportation of glucosinolates (GSL). The genes encoding GSL synthesis in leaves, such as basic helix-loop-helix, myelocytomatosis proteins, acetyl-CoA, cytochrome P450, and glutathione S-transferases, were significantly downregulated. In EG leaves, the contents of five of the eight amino acids involved in GSL synthesis decreased significantly (P < 0.05). Notably, total GSL in EG stems, leaves, and fruits had a significant linear correlation with total S and Cd. In summary, the decrease in total S and Cd content in scions caused by grafting is closely related to GSL. Our findings provide a theoretical basis for the safe use of Cd-contaminated soil, exploring the long-distance transport of Cd in plants and cultivating crops with low Cd accumulation.


Subject(s)
Soil Pollutants , Solanum melongena , Cadmium/analysis , Solanum melongena/metabolism , Glucosinolates/analysis , Antioxidants/metabolism , Soil , Soil Pollutants/analysis , Plant Roots/metabolism
3.
J Hazard Mater ; 457: 131811, 2023 09 05.
Article in English | MEDLINE | ID: mdl-37307733

ABSTRACT

Graphene oxide (GO)-promoted microbial degradation technology is considered an important strategy to eliminate polycyclic aromatic hydrocarbons (PAHs) in the environment; however, the mechanism by which GO affects microbial degradation of PAHs has not been fully studied. Thus, this study aimed to analyze the effect of GO-microbial interaction on PAHs degradation at the microbial community structure, community gene expression, and metabolic levels using multi-omics combined technology. We treated PAHs-contaminated soil samples with different concentrations of GO and analyzed the soil samples for microbial diversity after 14 and 28 days. After a short exposure, GO reduced the diversity of soil microbial community but increased potential degrading microbial abundance, promoting PAHs biodegradation. This promotion effect was further influenced by the GO concentration. In a short period of time, GO upregulated the expression of genes involved in microbial movement (flagellar assembly), bacterial chemotaxis, two-component system, and phosphotransferase system in the soil microbial community and increased the probability of microbial contact with PAHs. Biosynthesis of amino acids and carbon metabolism of microorganisms were accelerated, thereby increasing the degradation of PAHs. With the extension of time, the degradation of PAHs stagnated, which may be due to the weakened stimulation of GO on microorganisms. The results showed that screening specific degrading microorganisms, increasing the contact area between microorganisms and PAHs, and prolonging the stimulation of GO on microorganisms were important means to improve the biodegradation efficiency of PAHs in soil. This study elucidates how GO affects microbial PAHs degradation and provides important insights for the application of GO-assisted microbial degradation technology.


Subject(s)
Polycyclic Aromatic Hydrocarbons , Soil Pollutants , Soil Pollutants/metabolism , Polycyclic Aromatic Hydrocarbons/analysis , Soil Microbiology , Biodegradation, Environmental , Soil/chemistry
6.
Ecotoxicol Environ Saf ; 246: 114169, 2022 Nov.
Article in English | MEDLINE | ID: mdl-36228353

ABSTRACT

The expanding applications of lanthanides (Ln) in various aspects have raised concerns about their biosafety. Slight changes in the chemical composition of environmental media can significantly affect the biological effectiveness of poorly water-soluble Ln; however, the knowledge of the effects of environmental factors on Ln toxicity remains limited. Here, the effects of pH, HCO3-, Ca2+, Mg2+, Na+, K+, Cl-, and SO42- on the bioefficacy and biotoxicity of Ln (La, Ce, Gd, and Ho) were comparatively studied using zebrafish (Danio rerio) as the test organism. In the standard water, the toxicity of Ln in zebrafish was significantly correlated with pH, HCO3-, and Ca2+-Mg2+ levels in the medium but not with the levels of Cl-, Na+, K+, and SO42-. At the beginning of the test, the four Ln were complexed with HCO3- in the medium to form precipitates. A decrease in pH or HCO3- concentration can promote the conversion of granular Ln to a soluble state, thus enhancing their bioavailability, biotoxicity, and bioaccumulation. At a pH of 5.0 and 0.2 mmol·L-1 HCO3-, where Ln precipitates were not found, the four Ln showed a consistent trend of 96 h-LC50 in zebrafish. These data indicate that the differences in the toxicities of the four Ln in the standard water may be due to differences in the effective states of the individual elements rather than the different toxicities of the elements. Overall, in biological toxicity assessments, Ln can be regarded as a group of elements with additive patterns of toxicity until the differences in their biological toxicity mechanisms are revealed, and the effects of pH and carbonate should be considered.


Subject(s)
Lanthanoid Series Elements , Water Pollutants, Chemical , Animals , Zebrafish , Lanthanoid Series Elements/toxicity , Water Pollutants, Chemical/toxicity , Water Pollutants, Chemical/analysis , Ions , Water
7.
Chemosphere ; 308(Pt 3): 136561, 2022 Dec.
Article in English | MEDLINE | ID: mdl-36155022

ABSTRACT

Invasive plants could play an important role in the restoration of tailings, but their invasiveness limits their practical application. In this study, the phytoremediation potentials and invasive risks of an exotic invasive plant (Xanthium strumarium, LT), a native plant (X. sibiricum, CR), and combinations of inoculations (EG, with CR as the scion and LT as the rootstock; SG, with CR as both the scion and rootstock) were evaluated on Cd/Cu/Ni tailings. LT rootstock has a stronger nutrient and metal transport capacity, compared with CR. EG not only had higher biomass and Cd/Cu/Ni accumulation, but also abundant rhizosphere microbial communities. Hydroponic and common garden experiments showed that the growth and metal enrichment characteristics of EG are not inherited by plant offspring, which reduces the risk of the biological diffusion in the process of using exotic species. Transcriptome analysis shows that a large number of differentially-expressed genes in EG leaves and roots are involved in phenylpropanoid biosynthesis, secondary metabolite generation, and signal transduction. The genes induced in EG leaves, including cyclic nucleotide-gated ion channel, calcium-binding protein, and WRKY transcription factor, were found to be differentially expressed compared to CR. The genes induced in EG roots, included phenylalanine ammonia-lyase, cinnamoyl-CoA reductase, caffeoyl-CoA O-methyltransferase, and beta-glucosidase. We speculate that lignin and glucosinolates play an important role in the metal accumulation and transportation of EG. The results demonstrate that grafting with LT not only improved CR tolerance and accumulation of Cd, Cu, and Ni, but also created a beneficial microbial environment for plants in tailings. More importantly, grafting with LT did not enhance the invasiveness of CR. Our results provide an example of the safe use of invasive plants in the restoration of Cd/Cu/Ni tailings.


Subject(s)
Cellulases , Metals, Heavy , Soil Pollutants , Xanthium , Biodegradation, Environmental , Cadmium/analysis , Calcium-Binding Proteins/metabolism , Cellulases/metabolism , Copper/metabolism , Copper/toxicity , Glucosinolates/metabolism , Ion Channels/metabolism , Lignin/metabolism , Metals, Heavy/analysis , Nickel/metabolism , Nucleotides, Cyclic/metabolism , Phenylalanine Ammonia-Lyase/metabolism , Plants/metabolism , Soil Pollutants/analysis , Transcription Factors/metabolism
8.
Toxics ; 10(9)2022 Aug 31.
Article in English | MEDLINE | ID: mdl-36136485

ABSTRACT

The expanding applications of rare earth elements (REEs) in various fields have raised concerns about their biosafety. However, previous studies are insufficient to elucidate their toxic effects and mechanisms of action and whether there are uniform or predictable toxicity patterns among REEs. Herein, we investigated the toxic effects of two representative REEs (lanthanum (La) and gadolinium (Gd)) on zebrafish (Danio rerio) through toxicity experiments and transcriptome analysis. The results of the toxicity experiments showed that the two REEs have similar lethality, with half-lethal concentrations (LC50) at micromolar levels and mixed toxicity showing additive effects. Differential expression gene screening and functional group enrichment analysis showed that La and Gd might affect the growth and development of Danio rerio by interfering with some biological molecules. The two REEs showed significant effects on the metabolic pathways of exogenous or endogenous substances, including glutathione sulfotransferase and acetaldehyde dehydrogenase. Moreover, some basic biological processes, such as DNA replication, the insulin signaling pathway, and the p53 signaling pathway, were significantly enriched. Overall, the toxicity patterns of La and Gd may affect some biological processes with different intensities; however, there are many similarities in their toxicity mechanisms and modes of action. The concentrations investigated in this study were comparable to those of REE residues at highly contaminated sites, thus mimicking the ecotoxicological effects at environmentally relevant concentrations.

9.
Chemosphere ; 307(Pt 2): 135795, 2022 Nov.
Article in English | MEDLINE | ID: mdl-35917980

ABSTRACT

The continuous expansion of the application of rare earth elements (REEs) in various fields has attracted attention to their biosafety. At present, the molecular mechanisms underlying the biological effects of REEs are unclear. In this study, the effects of lanthanum (La) and gadolinium (Gd) on cell cycle progression in the root tips of rice seedlings were investigated. Low concentrations of REEs (0.1 mg L-1) induced an increase in the number of cells in the prophase and metaphase, while high concentrations of REEs (10 mg L-1) induced an increase in the number of cells in the late and terminal stages of the cell cycle, and apoptosis or necrosis. Additionally, low concentrations of REEs induced a significant increase in the expression of the cell cycle factors WEE1, CDKA;1, and CYCB1;1, and promoted the G2/M phase and accelerated root tip growth. However, at high REEs concentrations, the DNA damage response sensitized by BRCA1, MRE11, and TP53 could that prevent root tip growth by inhibiting the transcription factor E2F, resulting in obvious G1/S phase transition block and delayed G2/M phase conversion. Furthermore, by comparing the biological effect mechanisms of La and Gd, we found that these two REEs share regulatory actions on the cell cycle of root tips in rice seedlings.


Subject(s)
Metals, Rare Earth , Oryza , Cell Cycle , Cell Division , E2F Transcription Factors/metabolism , Gadolinium/pharmacology , Lanthanum/metabolism , Lanthanum/pharmacology , Meristem/metabolism , Metals, Rare Earth/pharmacology , Oryza/metabolism , Seedlings
10.
Sci Total Environ ; 847: 157488, 2022 Nov 15.
Article in English | MEDLINE | ID: mdl-35870595

ABSTRACT

Cadmium (Cd) pollution in farmland soil increases the probability of wastage of land resources and compromised food safety. Grafting can change the absorption rates of elements in crops; however, there are few studies on grafting in bulk grain and cash crops. In this study, Glycine max was used as a scion and Luffa aegyptiaca as a rootstock for grafting experiments. The changes in total sulfur and Cd content in the leaves and grains of grafted species were determined for three consecutive generations, and the gene expression and DNA methylation status of the leaves were analyzed. The results show that grafting significantly reduced the total sulfur and Cd content in soybean leaves and grains; the Cd content in soybean leaves and grains decreased by >50 %. The plant's primary sulfur metabolism pathway was not significantly affected. Glucosinolates and DNA methylation may play important roles in reducing total sulfur and Cd accumulation. Notably, low sulfur and low Cd traits can be maintained over two generations. Our study establishes that grafting can reduce the total sulfur and Cd content in soybean, and these traits can be inherited. In summary, grafting technology can be used to prevent soybean from accumulating Cd in farmland soil. This provides a theoretical basis for grafting to cultivate crops with low Cd accumulation.


Subject(s)
Oryza , Soil Pollutants , Cadmium/analysis , Crops, Agricultural/metabolism , DNA Methylation , Glucosinolates/metabolism , Oryza/metabolism , Soil , Soil Pollutants/analysis , Glycine max/genetics , Glycine max/metabolism , Sulfur/metabolism
11.
Ecotoxicol Environ Saf ; 215: 112138, 2021 Jun 01.
Article in English | MEDLINE | ID: mdl-33740487

ABSTRACT

Rare Earth Elements (REEs) are increasingly being used in agriculture and are also used to produce high end technological devices, thereby increasing their anthropogenic presence in the environment. However, the ecotoxicological mechanism of REEs on organisms is not fully understood. In this study, the effects of gadolinium (Gd) addition on Arabidopsis thaliana (L.) were investigated at both physiological and molecular levels. Four treatments (0, 10, 50 and 200 µmol·L-1 Gd) were used in the exposure tests. Biomass, root length and chlorophyll content in shoots/roots were measured to investigate the plant's physiological response to Gd stress. Random amplified polymorphic (RAPD)-Polymerase Chain Reaction (PCR) and methylation sensitive arbitrarily primed (MSAP)-PCR were used to investigate changes in genetic variation and DNA methylation of A. thaliana when exposed to Gd. At the physiological level, it was found that low concentration of Gd (10 µmol·L-1) could significantly increase the plant biomass and root length, while the growth of A. thaliana was significantly inhibited when exposed to 200 µmol·L-1 of Gd, yet the total soluble protein content in aerial plant parts increased significantly by 24.2% when compared to the control group. Among the 12 primers considered in the RAPD assessment, at the molecular level, only four primers revealed different patterns in their genomic DNA. Compared to the control group, the treatment with 50 µmol·L-1 of Gd was associated with lower polymorphism, while the treatment with 200 µmol·L-1 of Gd was associated with higher polymorphism. The polymorphism frequencies for the 50 µmol·L-1 of Gd and the 200 µmol·L-1 of Gd were 4.67% and 20.33%, respectively. The MSAP analysis revealed that the demethylation (D) type of Arabidopsis genomic DNA increased significantly under 10 and 50 µmol·L-1 of Gd, while the methylation (M) type was also significantly increased under 200 µmol·L-1 of Gd. Generally, the total methylation polymorphism (D+M) increased with an increase of Gd concentration. It was found that high concentrations of Gd appeared to cause DNA damage, but low concentrations of Gd (as low as 10 µmol·L-1) were associated with DNA methylation change. Further, it was verified by Real time Reverse Transcription PCR (RT-PCR) on the bands detected by the MSAP analysis, that the genes relative to processes including cell cycle, oxidative stress and apoptosis, appeared to be regulated by methylation under Gd stress. These findings reveal new insight regarding ecotoxicity mechanisms of REEs on plants.


Subject(s)
Arabidopsis/physiology , Environmental Pollutants/toxicity , Gadolinium/toxicity , Arabidopsis/metabolism , Chlorophyll/metabolism , DNA Damage , DNA Methylation , Ecotoxicology , Gadolinium/metabolism , Metals, Rare Earth , Oxidative Stress , Plant Roots/metabolism , Random Amplified Polymorphic DNA Technique
12.
Sci Total Environ ; 652: 989-995, 2019 Feb 20.
Article in English | MEDLINE | ID: mdl-30380503

ABSTRACT

Heavy metal cadmium (Cd) pollution in farmland has become a serious threat to food security globally. In this work, a grafting technique was applied to eggplant (Solanum melongena) and tomato (Solanum lycopersicum) plants using Solanum torvum as rootstock to investigate effects of grafting on Cd accumulation in shoots. The un-grafted, self-grafted, and grafted plants were grown in soils containing 2 mg kg-1 Cd. Results showed that grafting on S. torvum could efficiently reduce Cd accumulation in leaves of eggplant and tomato, and the decrease was 89% and 72%, respectively. With S. torvum as rootstock, Cd concentrations were 1.11 mg kg-1 and 6.58 mg kg-1 in leaves of grafted eggplant and tomato, which were significantly decreased as compared with un-grafted plants (10.12 mg kg-1 and 23.19 mg kg-1, respectively, p < 0.05). In addition, Cd concentrations were 12.11 mg kg-1 and 29.47 mg kg-1 in leaves of self-grafted eggplant and tomato, respectively, which was similar to those in un-grafted eggplant, but more than those in un-grafted tomato (p < 0.05). This suggests that the S. torvum rootstock, and not the grafting operation, was responsible for efficient reduction of Cd accumulation in shoots of eggplant and tomato plants. Furthermore, total sulfur and sulfate (SO42-) concentrations analysis revealed that there was a similar trend between Cd accumulation and total sulfur or SO42- concentrations in leaves of plants tested. Additionally, a strong positive correlation between Cd accumulation and total sulfur or SO42- concentrations occurred in leaves of eggplant and tomato plants. Thus, sulfur, mainly SO42-, in leaves may play an important regulatory role in Cd accumulation of eggplant and tomato plants. This study provides the theoretical and technical support for applying grafting technique for the safe practice of farming in Cd-contaminated agricultural soil.


Subject(s)
Cadmium/metabolism , Soil Pollutants/metabolism , Solanum lycopersicum/metabolism , Solanum melongena/metabolism
13.
Ecotoxicol Environ Saf ; 147: 656-663, 2018 Jan.
Article in English | MEDLINE | ID: mdl-28934709

ABSTRACT

Water quality criteria (WQC) are considered to be an effective management tool for protecting aquatic environments. To derive site-specific WQC for an area, local data based on local species are essential to improve the applicability of WQC derived. Due to the paucity of local fish data available for the development of site-specific WQC for the Liao River, China, four local and widespread fishes (Pseudorasbora parva, Abbottina liaoningensis, Ctenogobius giurinus, and Misgurnus anguillicaudatus) were chosen to test their sensitivities to ammonia, cadmium and nitrobenzene. These compounds are common and regularly-measured pollutants in Chinese rivers. In addition to the published data for species resident in the Liao River, site-specific WQC for the three chemicals were derived using both a log-logistic species sensitivity distribution (SSD) and the method recommended by the USEPA, in line with current best practice, which were then compared with Chinese national WQC. It was found that A. liaoningensis was the most sensitive, followed, in order, by P. parva, C. giurinus and M. anguillicaudatus was the least sensitive, and this trend was the same to all three chemicals tested. When comparing the SSD derived solely from previously-published data with that including our data on local fish, there were significant differences identified among parameters describing the SSD curves for ammonia and nitrobenzene and significant differences were detected for site-specific WQC derived for all of the three chemicals. Based on the dataset with local fish data taxa, site-specific WQC of Liao River for ammonia, cadmium, and nitrobenzene were derived to be 20.53mg/L (at a pH of 7.0 and temperature of 20°C), 3.76µg/L (at a hardness of 100mg/L CaCO3), and 0.49mg/L, respectively. Using the same deriving method for each chemical, the national Chinese WQC were higher than site-specific WQC derived in this study for ammonia (national WQC of 25.16mg/L) and nitrobenzene (national WQC of 0.57mg/L), while the national WQC for cadmium was lower (national WQC of 1.81µg/L). These results indicated that published data can be helpful for use when deriving site-specific WQC but that there were differences between site-specific and national WQC which may lead to either over- or under-protection depending on the pollutant if national WQC were used as the basis for the water management of specific river systems, like the Liao River.


Subject(s)
Ammonia/toxicity , Cadmium/toxicity , Fishes/growth & development , Nitrobenzenes/toxicity , Rivers/chemistry , Water Pollutants, Chemical/toxicity , Water Quality/standards , Ammonia/analysis , Animals , Cadmium/analysis , China , Lethal Dose 50 , Nitrobenzenes/analysis , Species Specificity , Toxicity Tests , Water Pollutants, Chemical/analysis
14.
Ecotoxicol Environ Saf ; 145: 569-574, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28800532

ABSTRACT

Accumulating evidence demonstrates that the aberrant expression of cell cycle regulation and DNA repair genes can result in abnormal cell proliferation and genomic instability in eukaryotic cells under different stresses. Herein, Arabidopsis thaliana (Arabidopsis) seedlings were grown hydroponically on 0.5 × MS media containing cadmium (Cd) at 0-2.5mgL-1 for 5d of treatment. Real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that expression of DNA damage repair and cell cycle regulation genes, including BRCA1, MRE11, WEE1, CDKA;1 and PCNA1, showed an inverted U-shaped dose-response. In contrast, notably reduced expression was observed for G1-to-S transition-related genes, Histone H4, E2Fa and PCNA2; DSB end processing, GR1; G2-to-M transition-related gene, CYCB1;1; and DNA mismatch repair, MSH2, MSH6 and MLH1 genes in root tips exposed to 0.125-2.5mg/L Cd for 5d. Flow cytometry (FCM) analysis revealed significant increases of cells with a 2C nuclear content and with a 4C and 8C nuclear content under Cd stresses of 0.125 and 1-2.5mgL-1, respectively. Our results suggest that 0.125mgL-1 Cd-induced DNA damage induced the marked G1/S arrest, leading to accelerated growth in root tips, while 1.0-2.5mgL-1 Cd-induced DNA damage caused a notable G2/M arrest in root tips, leading to reduced growth in root tips. This may be a protective mechanism that prevents cells with damaged DNA from dividing under Cd stress.


Subject(s)
Arabidopsis/drug effects , Cadmium/toxicity , Cell Cycle Checkpoints/drug effects , DNA Damage , Meristem/drug effects , Soil Pollutants/toxicity , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Cell Cycle Checkpoints/genetics , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Genomic Instability/drug effects , Meristem/genetics , Seedlings/drug effects , Seedlings/genetics
15.
Chemosphere ; 150: 258-265, 2016 May.
Article in English | MEDLINE | ID: mdl-26907594

ABSTRACT

Microsatellite instability (MSI) analysis, random-amplified polymorphic DNA (RAPD), and methylation-sensitive arbitrarily primed PCR (MSAP-PCR) are methods to evaluate the toxicity of environmental pollutants in stress-treated plants and human cancer cells. Here, we evaluate these techniques to screen for genetic and epigenetic alterations of Arabidopsis plantlets exposed to 0-5.0 mg L(-1) cadmium (Cd) for 15 d. There was a substantial increase in RAPD polymorphism of 24.5, and in genomic methylation polymorphism of 30.5-34.5 at CpG and of 14.5-20 at CHG sites under Cd stress of 5.0 mg L(-1) by RAPD and of 0.25-5.0 mg L(-1) by MSAP-PCR, respectively. However, only a tiny increase of 1.5 loci by RAPD occurred under Cd stress of 4.0 mg L(-1), and an additional high dose (8.0 mg L(-1)) resulted in one repeat by MSI analysis. MSAP-PCR detected the most significant epigenetic modifications in plantlets exposed to Cd stress, and the patterns of hypermethylation and polymorphisms were consistent with inverted U-shaped dose responses. The presence of genomic methylation polymorphism in Cd-treated seedlings, prior to the onset of RAPD polymorphism, MSI and obvious growth effects, suggests that these altered DNA methylation loci are the most sensitive biomarkers for early diagnosis and risk assessment of genotoxic effects of Cd pollution in ecotoxicology.


Subject(s)
Arabidopsis/drug effects , Cadmium/toxicity , DNA Methylation/drug effects , Ecotoxicology/methods , Environmental Pollutants/toxicity , Microsatellite Instability/drug effects , Arabidopsis/genetics , Biomarkers/analysis , DNA Damage , Polymorphism, Genetic/drug effects , Random Amplified Polymorphic DNA Technique , Seedlings/drug effects , Seedlings/genetics
16.
Chemosphere ; 89(9): 1048-55, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22717160

ABSTRACT

Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, Arabidopsis thaliana (Arabidopsis) plantlets following Cadmium (Cd) treatment for 26 d were screened for DNA genetic alterations by DNA fingerprinting. Four primers amplified 20-23 mutated RAPD fragments in 0.125-3.0 mg L(-1) Cd-treated Arabidopsis plantlets, respectively. Cloning and sequencing analysis of eight randomly selected mutated fragments revealed 99-100% homology with the genes of VARICOSE-Related, SLEEPY1 F-box, 40S ribosomal protein S3, phosphoglucomutase, and noncoding regions in Arabidopsis genome correspondingly. The results show the ability of RAPD analysis to detect significant genetic alterations in Cd-exposed seedlings. Although the exact functional importance of the other mutated bands is unknown, the presence of mutated loci in Cd-treated seedlings, prior to the onset of significant physiological effects, suggests that these altered loci are the early events in Cd-treated Arabidopsis seedlings and would greatly improve environmental risk assessment.


Subject(s)
Arabidopsis/drug effects , Cadmium/toxicity , Soil Pollutants/toxicity , Arabidopsis/genetics , Arabidopsis/physiology , DNA Damage , DNA Fingerprinting , Mutation , Seedlings/drug effects , Seedlings/physiology
SELECTION OF CITATIONS
SEARCH DETAIL
...