ABSTRACT
MOTIVATION: With the sites of antigen expression different, the segmentation of immunohistochemical (IHC) histopathology images is challenging, due to the visual variances. With H&E images highlighting the tissue structure and cell distribution more broadly, transferring more salient features from H&E images can achieve considerable performance on expression site agnostic IHC images segmentation. METHODS: To the best of our knowledge, this is the first work that focuses on domain adaptive segmentation for different expression sites. We propose an expression site agnostic domain adaptive histopathology image semantic segmentation framework (ESASeg). In ESASeg, multi-level feature alignment encodes expression site invariance by learning generic representations of global and multi-scale local features. Moreover, self-supervision enhances domain adaptation to perceive high-level semantics by predicting pseudo-labels. RESULTS: We construct a dataset with three IHCs (Her2 with membrane stained, Ki67 with nucleus stained, GPC3 with cytoplasm stained) with different expression sites from two diseases (breast and liver cancer). Intensive experiments on tumor region segmentation illustrate that ESASeg performs best across all metrics, and the implementation of each module proves to achieve impressive improvements. CONCLUSION: The performance of ESASeg on the tumor region segmentation demonstrates the efficiency of the proposed framework, which provides a novel solution on expression site agnostic IHC related tasks. Moreover, the proposed domain adaption and self-supervision module can improve feature domain adaption and extraction without labels. In addition, ESASeg lays the foundation to perform joint analysis and information interaction for IHCs with different expression sites.
