ABSTRACT
The Tradescantia micronucleus test is a sensitive bioassay for mutagenesis that may be employed both under field and laboratory conditions. This test has been standardized mostly on the basis of the results obtained with clone 4430. However, this clone is not well adapted to tropical weather, frequently showing problems with growth and flowering. In addition, it is attacked by parasites and insects, a fact that limits its use in field studies aiming at the biomonitoring of air pollution. In the city of Säo Paulo, Tradescantia pallida (Rose) Hunt. var. purpurea Boom is widely distributed as an ornamental plant in gardens and along roadsides and streets, mostly because of its natural resistance and its easy propagation. In this report, we present dose-response curves indicating that the sensitivity of T. pallida and clone 4430 to X-radiation (1, 10, 25 and 50 cGy) is similar. The results confirm our previous suggestion that T. pallida represents a good alternative for in situ mutagenesis testing in tropical regions, especially biomonitoring studies in which the exposure conditions may not be fully controllable
Subject(s)
Plants , Pollen , Rosales , Micronucleus Tests/methods , Clone Cells , Plants , Pollen , Dose-Response Relationship, Radiation , Sensitivity and Specificity , X-RaysABSTRACT
The Tradescantia micronucleus test is a sensitive bioassay for mutagenesis that may be employed both under field and laboratory conditions. This test has been standardized mostly on the basis of the results obtained with clone 4430. However, this clone is not well adapted to tropical weather, frequently showing problems with growth and flowering. In addition, it is attacked by parasites and insects, a fact that limits its use in field studies aiming at the biomonitoring of air pollution. In the city of São Paulo, Tradescantia pallida (Rose) Hunt. var. purpurea Boom is widely distributed as an ornamental plant in gardens and along roadsides and streets, mostly because of its natural resistance and its easy propagation. In this report, we present dose-response curves indicating that the sensitivity of T. pallida and clone 4430 to X-radiation (1, 10, 25 and 50 cGy) is similar. The results confirm our previous suggestion that T. pallida represents a good alternative for in situ mutagenesis testing in tropical regions, especially biomonitoring studies in which the exposure conditions may not be fully controllable.
Subject(s)
Micronucleus Tests/methods , Plants/radiation effects , Pollen/radiation effects , Rosa/radiation effects , Clone Cells , Dose-Response Relationship, Radiation , Plants/genetics , Pollen/genetics , Rosa/genetics , Sensitivity and Specificity , X-RaysABSTRACT
Endoscopic Retrograde Choledocho-Pancreatography, Ultrasonography and Computer Tomography can show dilated pancreatic duct in the patients with small resectable pancreatic carcinoma. However they can not predict the size of the tumor. Angiography is a complementary examination, being able to demonstrate the size of the tumor and predict its resectability.