Treatment of the liquid phase of digestate from a biogas plant for water reuse.

Biogas plants struggle with managing nitrogen-rich digestate from manure co-digestion. In this study, the biologically treated liquid phase of digestate from an aerobic granular sludge batch reactor (GSBR) containing oxidized nitrogen forms (NOx), phosphorus, COD and total suspended solids was post-denitrified (P-D), and then ultrafiltered. In P-D, various hydraulic retention times (from 10 to 60 h) and biomass concentrations (from 6 to 14 g MLSS/L) were tested. Then, waste glycerin (GL) was added to the P-D reactor at a CODGL/NOx ratio of 1.1, causing a large number of phosphate-accumulating and denitrifying Janibacter sp., and PHB-accumulating and denitrifying Paracoccus sp. and Thauera sp. to be present in granules, which improved nutrient removal. The effluent was ultrafiltered at 0.3 and 0.5 MPa. After biological treatment supported with GL and followed by ultrafiltration, the purified liquid phase of the digestate met FAO standards for water reuse for irrigation.

Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads.

This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR). The organic loadings were 0.78 kg COD/(m3·d) (GSBR1), 1.16 kg COD/(m3·d) (GSBR2) and 1.53 kg COD/(m3·d) (GSBR3). Granules with a diameter of 0.5-1 mm had the most stable EPS content and composition. The smallest granules had the largest amount of bound EPS. The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle. The proteins/polysaccharides ratio decreased below 1 only in soluble EPS in the famine period. In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.

Treatment of Ammonium-Rich Digestate from Methane Fermentation Using Aerobic Granular Sludge.

Digestate produced by cofermentation of agricultural waste and manure can be difficult to dispose of because its high ammonium content impedes its use in agriculture due to generation of odor and overfertilization. This study investigated the possibility of treating such nitrogen-rich digestate with aerobic granular sludge depending on the nitrogen load in the reactor. At nitrogen loading rate of 1.0 g TN/(L·day), the nitrogen removal efficiency was high (64.9 ± 9.8%), ammonium nitrogen was completely oxidized, and nitrate was the main nitrification product. At nitrogen loading rate of 3.4 g TN/(L·day), ammonium oxidization was still good (93.6 ± 2.0%), but the percentage of partial nitrification was high (over 68%) and nitrogen removal efficiency worsened to 30.2 ± 2.6%. Despite this, the overall amount of nitrogen removed was 0.86 g TN/(L·day) and was over nearly two times higher than at the lower nitrogen loading rate. At both nitrogen loading rates, in the effluent nitrogen in a form of suspended solids predominated. To diminish the overall N loading in the effluent, treatment is therefore recommended enabling removal of solids, e.g., microfiltration, should be applied, or the digestate should be separated into solid and liquid phases, and only the liquid fraction should be subjected to biological treatment. At high N load in aerobic granules, a very versatile community of N-metabolizing microorganisms was present. More than 50% of all bacteria in aerobic granules were able to metabolize nitrogen, and the predominant genera (35%) was Thauera, which indicated that stable ammonium removal was achieved mostly as a result of heterotrophic nitrification.

Performance and microbial characteristics of biomass in a full-scale aerobic granular sludge wastewater treatment plant.

By modification of the operational conditions of batch reactors, a municipal wastewater treatment plant was upgraded from activated sludge to aerobic granular sludge (AGS) technology. After upgrading, the volume of the biological reactors was reduced by 30%, but the quality of the effluent substantially improved. The concentration of biomass in the reactors increased twofold; the average biomass yield was 0.6 g MLVSS/g COD, and excess granular sludge was efficiently stabilized in aerobic conditions. Canonical correspondence analysis based on the results of next-generation sequencing showed that the time of adaptation significantly influenced the microbial composition of the granules. In mature granules, the abundance of ammonium-oxidizing bacteria was very low, while the abundance of the nitrite-oxidizing bacteria Nitrospira sp. was 0.5 ± 0.1%. The core genera were Tetrasphaera, Sphingopyxis, Dechloromonas, Flavobacterium, and Ohtaekwangia. Bacteria belonging to these genera produce extracellular polymeric substances, which stabilize granule structure and accumulate phosphorus. The results of this study will be useful for designers of AGS wastewater treatment plants, and molecular data given here provide insight into the ecology of mature aerobic granules from a full-scale facility.

Temperature-induced changes in treatment efficiency and microbial structure of aerobic granules treating landfill leachate.

This paper investigates the effect of temperature on nitrogen and carbon removal by aerobic granules from landfill leachate with a high ammonium concentration and low concentration of biodegradable organics. The study was conducted in three stages; firstly the operating temperature of the batch reactor with aerobic granules was maintained at 29 °C, then at 25 °C, and finally at 20 °C. It was found that a gradual decrease in operational temperature allowed the nitrogen-converting community in the granules to acclimate, ensuring efficient nitrification even at ambient temperature (20 °C). Ammonium was fully removed from leachate regardless of the temperature, but higher operational temperatures resulted in higher ammonium removal rates [up to 44.2 mg/(L h) at 29 °C]. Lowering the operational temperature from 29 to 20 °C decreased nitrite accumulation in the GSBR cycle. The highest efficiency of total nitrogen removal was achieved at 25 °C (36.8 ± 10.9 %). The COD removal efficiency did not exceed 50 %. Granules constituted 77, 80 and 83 % of the biomass at 29, 25 and 20 °C, respectively. Ammonium was oxidized by both aerobic and anaerobic ammonium-oxidizing bacteria. Accumulibacter sp., Thauera sp., cultured Tetrasphaera PAO and Azoarcus-Thauera cluster occurred in granules independent of the temperature. Lower temperatures favored the occurrence of denitrifiers of Zooglea lineage (not Z. resiniphila), bacteria related to Comamonadaceae, Curvibacter sp., Azoarcus cluster, Rhodobacter sp., Roseobacter sp. and Acidovorax spp. At lower temperatures, the increased abundance of denitrifiers compensated for the lowered enzymatic activity of the biomass and ensured that nitrogen removal at 20 °C was similar to that at 25 °C and significantly higher than removal at 29 °C.