The bacterial and archaeal communities of flies, manure, lagoons, and troughs at a working dairy.

Background: Fundamental investigations into the location, load, and persistence of microbes, whether beneficial or detrimental, are scarce. Many questions about the retention and survival of microbes on various surfaces, as well as the load necessary for spread, exist. To answer these questions, we must know more about where to find various microbes and in what concentrations, the composition of the microbial communities, and the extent of dissemination between various elements. This study investigated the diversity, composition, and relative abundance of the communities associated with manure, lagoons, troughs, house flies, and stable flies present at a dairy, implementing two different free-stall management systems: flow-through and cross-vent. Shotgun metagenomics at the community level was used to compare the microbiomes within the dairy, allowing confident interpretation at the species level. Results: The results showed that there were significant difference in microbial composition between not only each of the dairy elements but also management styles. The primary exceptions were the microbiomes of the house fly and the stable fly. Their compositions heavily overlapped with one another, but interestingly, not with the other components sampled. Additionally, both species of flies carried more pathogens than the other elements of the dairy, indicating that they may not share these organisms with the other components, or that the environments offered by the other components are unsatisfactory for the survival of some pathogens.. Conclusion: The lack of overlapping pathogen profiles suggests a lack of transfer from flies to other dairy elements. Dairy health data, showing a low incidence of disease, suggests minimal sharing of bacteria by the flies at a level required for infection, given the health program of this dairy. While flies did carry a multitude of pathogenic bacteria, the mere presence of the bacteria associated with the flies did not necessarily translate into high risk leading to morbidity and mortality at this dairy. Thus, using flies as the sole sentinel of dairy health may not be appropriate for all bacterial pathogens or dairies.

No Evidence of SARS-CoV-2 Among Flies or Cockroaches in Households Where COVID-19 Positive Cases Resided.

Flies and other arthropods mechanically transmit multiple pathogens and a recent experimental study demonstrated house flies, Musca domestica L. (Diptera: Muscidae), can mechanically transmit SARS-CoV-2. The purpose of this study was to explore the possibility of mechanical transmission of SARS-CoV-2 by domestic insects and their potential as a xenosurveillance tool for detection of the virus. Flies were trapped in homes where at least one confirmed human COVID-19 case(s) resided using sticky and liquid-baited fly traps placed inside and outside the home in the Texas counties of Brazos, Bell, and Montgomery, from June to September 2020. Flies from sticky traps were identified, pooled by taxa, homogenized, and tested for the presence of SARS-CoV-2 RNA using quantitative reverse transcription PCR (RT-qPCR). Liquid traps were drained, and the collected fluid similarly tested after RNA concentration. We processed the contents of 133 insect traps from 40 homes, which contained over 1,345 individual insects of 11 different Diptera families and Blattodea. These individuals were grouped into 243 pools, and all tested negative for SARS-CoV-2 RNA. Fourteen traps in seven homes were deployed on the day that cat or dog samples tested positive for SARS-CoV-2 RNA by nasal, oral, body, or rectal samples. This study presents evidence that biting and nonbiting flies and cockroaches (Blattodea) are not likely to contribute to mechanical transmission of SARS-CoV-2 or be useful in xenosurveillance for SARS-CoV-2.

The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.

BACKGROUND: The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. RESULTS: This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. CONCLUSIONS: The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha.

Distribution of Culex coronator in Texas.

The distribution of Culex coronator Dyar and Knab by county in Texas was updated by combining data from peer-reviewed literature, military and government reports, and university and private collections, and by collecting specimens from counties where data had not been reported. With 254 counties in Texas, the initial collection effort was focused on counties east of US Highway 277, which runs from Val Verde County on the US and Mexico border to Wichita County on the Texas and Oklahoma border. The study resulted in 127 counties with Cx. coronator presence data. The remaining 127 counties need to be surveyed for this species.

Susceptibility of Alphitobius diaperinus in Texas to permethrin- and ß-cyfluthrin-treated surfaces.

BACKGROUND: Effective control of the lesser mealworm beetle, Alphitobius diaperinus, relies heavily on insecticides. The susceptibility level of beetles to these insecticides can be dependent on active ingredient, population treated, formulation, surface treated and timing of observation. The susceptibility of adult beetles from six populations to ß-cyfluthrin was determined up to 48 h after exposure. The susceptibility of adult beetles to the label rate of ß-cyfluthrin and permethrin formulations on concrete, wood-chip-type particle board and pressure-treated wood was determined up to 48 h post-exposure. RESULTS: Variation in LC50 values at 2 and 24 h was found within and between beetle populations from two regions of Texas. The permethrin formulation had lower mean mortality than the ß-cyfluthrin formulation on all surfaces tested. The permethrin formulation had high levels of recovery on all surfaces tested after 2 h. Surface affected the efficacy of the insecticides tested on killing adult beetles. CONCLUSION: Permethrin-based insecticide had lower knockdown and persistence on various surfaces over time than ß-cyfluthrin-based insecticide. Beetle recovery in less susceptible populations may necessitate longer observation periods for efficacy evaluations. Our study also shows that surfaces chosen can affect the efficacy of the compound on killing adult beetles. © 2016 Society of Chemical Industry.