Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Microb Biotechnol ; 5(2): 241-50, 2012 Mar.
Article in English | MEDLINE | ID: mdl-21895998

ABSTRACT

This study is focusing on elucidation of the capacity of attenuated Salmonella enteritidis E23 (cya, crp) to serve as a vehicle for the rectal delivery of the DNA vaccine. Earlier for creation HIV-1 candidate DNA vaccine we have designed the polyepitope protein TCI (T-cell immunogen), which comprises over 80 CTL epitopes from subtype A, B and C HIV-1 proteins. The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI. The attenuated S. enteritidis E23 was transformed by electroporation with recombinant plasmid pcDNA-TCI and the expression of the TCI gene was determined in vitro and in vivo. BALB/c mice were rectally immunized with S. enteritidis E23/pcDNA-TCI (108 cfu) twice at 4 week interval. Bacteria were not pathogenic for mice and spontaneously eliminated from mice spleen and liver to 60 days post the immunization. Detectable antibodies were generated in 2 weeks after immunization and their level increased after second immunization. The results of INF-γ ELISpot show that mice immunized with S. enteritidis E23/pcDNA-TCI elicited HIV-specific cellular immune response. This study demonstrates that attenuated S. enteritidis E23 is an effective live vector for rectal delivery of the DNA vaccine pcDNA-TCI to generate humoral and T-cellular responses against HIV-1.


Subject(s)
AIDS Vaccines/administration & dosage , Drug Delivery Systems , Epitopes, T-Lymphocyte/immunology , Genetic Vectors , HIV-1/immunology , Salmonella enteritidis/genetics , Vaccines, DNA/administration & dosage , AIDS Vaccines/genetics , AIDS Vaccines/immunology , Administration, Rectal , Animals , Enzyme-Linked Immunospot Assay , Epitopes, T-Lymphocyte/genetics , HIV Antibodies/blood , HIV-1/genetics , Interferon-gamma/metabolism , Leukocytes, Mononuclear/immunology , Liver/microbiology , Mice , Mice, Inbred BALB C , Spleen/microbiology , Vaccination/methods , Vaccines, Attenuated/administration & dosage , Vaccines, Attenuated/genetics , Vaccines, Attenuated/immunology , Vaccines, DNA/genetics , Vaccines, DNA/immunology
SELECTION OF CITATIONS
SEARCH DETAIL