The effects of road salt on freshwater ecosystems and solutions for mitigating chloride pollution - A review.

Road salt (mainly NaCl) is commonly used during the winter to ensure road and pavement safety; however, the long-term application of NaCl has negative consequences on soil and the water environment. The aims of the present review were to evaluate the impact of road salt on catchment processes which accelerate the eutrophication of waters, and to identify a possible approach for reducing the impact of winter salt treatments of roads and sidewalks, on water body quality. The objectives were implemented in accordance with the ecohydrological approach, which recommends using hierarchical steps to solve problems. The first step was the monitoring of threats, in which the causes of high chloride (Cl) concentrations in groundwater and surface water were identified. The results indicate that long-term winter application of road salt increases the annual mean concentrations of Cl in rivers and lakes, due to Cl entering groundwater. The second step was a cause-effect analysis of the impact of NaCl on the abiotic processes in soil and water, and on the biotic response to chloride exposure. Chlorides appear to decrease the biodiversity of aquatic animals and plants but favour the growth of phytoplankton, especially cyanobacteria. Moreover, Cl reduces the self-purification processes of water by decreasing nutrient accumulation in macrophytes, decreasing the denitrification rate and reducing organic matter decomposition. The third step was to evaluate possible solutions for reducing the negative impact of NaCl on the environment, and to improve the effectiveness of alternative de-icing agents. An analysis of available literature indicates that a system-based approach integrating engineering knowledge with an understanding of biological and hydrological processes is necessary to indicate solutions for reducing environmental risks from road salt use.

The impact of WWTP size and sampling season on the prevalence of antibiotic resistance genes in wastewater and the river system.

There is a growing concern about the fate of antibiotic resistance genes (ARGs) during wastewater treatment and their potential impacts on the receiving water bodies. We hypothesised that the quantity of ARGs in effluents may be related to the size of wastewater treatment plants (WWTPs) and sampling season. To date, only several attempts have been made to investigate the impact of the above factors at the catchment scale. Therefore, the goal of the present study was to explore possible differences in the quantity of ARGs in treated wastewater from small, medium-sized and large WWTPs in the catchment of the Pilica River (9258 km2). The impact of treated wastewater on the concentration of ARGs was also determined along the river continuum from upland to lowland segments to the point of confluence with the Vistula (342 km). Treated effluent was sampled in 17 WWTPs, and river water was sampled in 7 sampling sites in four seasons. The concentrations of blaTEM, tet(A), ermF, sul1 and aac(6')-Ib-cr genes, the integrase gene intI1 and the 16S rRNA gene were analysed by quantitative PCR. The physical and chemical parameters and nutrient concentrations (23 various parameters) in the analysed samples were determined. The highest absolute concentrations of the studied genes were noted in effluent samples from small WWTPs (p < 0.01). The concentration of ARGs (gene copies/mL) peaked in winter and spring samples (p < 0.04). The results of statistical analyses indicate that in small WWTPs, the absolute concentration of ARGs can be predicted based on the biochemical oxygen demand, in routine water analyses. However, none of the studied parameters supported predictions of ARG abundance in medium-sized and large WWTPs or in river water.