Relation between Shear Stresses and Flexural Tensile Stresses from Standardized Tests of Extracted Prismatic Specimens of an SFRC Bridge Girder.

Experimental research on the direct shear behavior of fiber-reinforced concrete is often carried out using prisms molded with specific dimensions for a standardized test. However, the flow of fresh concrete in these molds can be different than in the case of a full-scale structural element. This is important considering that the flow direction highly influences the distribution and orientation of fibers. In addition, most of the studies did not relate their shear results to other mechanical properties. In contrast, this study attempted to deepen the experimental knowledge of the crack propagation of a steel fiber-reinforced concrete (SFRC) used in a full-scale prototype of a bridge box girder built in the laboratory. Prismatic specimens were sawn from webs and top flanges of this prototype. Serving as references, additional specimens were molded in wooden boxes. In a previous study of our research group, both had been tested under a three-point notched bending configuration maintaining test conditions proportional to the EN14651 specifications. From each of the previously flexurally tested specimens, two prismatic specimens suitable for the Fédération Internationale de la Précontrainte (FIP) shear test setup were extracted by adopting a cutting methodology that avoided the damage induced by the flexural tests to be part of the FIP specimens. These FIP specimens were tested in almost pure shear loading conditions for assessing the performance of SFRC. Computer tomography images and photos of the shear failure faces were used to determine the distribution and density of fibers. The results demonstrated that the peak loads were proportional to the fiber density at the shear failure section. Assuming that the SFRC conditions of the webs were representative of a common batching procedure in the construction industry, the results from the tests in specimens extracted from these webs were adopted to establish shear stress/flexural tensile stress ratios vs. crack mouth opening displacement curves. The curves belonging to cross-sections of a similar fiber density in the shear and flexural cases allowed for the proposal of a normalized crack-dilatancy relation composed of three stages of the crack propagation. In addition, a trilinear crack width-slip relation was established using the same set of specimens. The relevancy of this proposal is that the shear response can be estimated from a widely accepted standardized flexural test, which demands a simpler instrumentation and is also easier to execute than the shear setup.

The bacterial microbiome and metabolome in caries progression and arrest.

Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the caries-inducing protocol for 4- and 6-weeks, and subsequently promoted caries lesion arrest via a 2-week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies.

Tritium ((3)H) as a tracer for monitoring the dispersion of conservative radionuclides discharged by the Angra dos Reis nuclear power plants in the Piraquara de Fora Bay, Brazil.

Presently, two nuclear power plants operate in Brazil. Both are located at Itaorna beach, Angra dos Reis, approximately 133 km from Rio de Janeiro city. The reactor cooling circuits require the input of seawater, which is later discharged through a pipeline into the adjacent Piraquara de Fora Cove. The radioactive effluents undergo ion-exchange treatment prior to their release in batches, causing the enrichment of (3)H relative to other radionuclides in the discharged waters. Under steady state conditions, the (3)H gradient in the Piraquara de Fora waters can be used to determine the dependence of the dilution factor on the distance from the discharge point. The present work describes experiments carried out at the reactor site during batch release episodes, including time series sampling at the discharge point and surface seawater sampling every 250 m to a distance of 1250 m, after a double distillation, the (3)H concentration was measured by liquid scintillation counting applying a Quantulus liquid scintillation spectrometer. The obtained results showed a linear relationship between the (3)H concentration and distance from the discharge point. At 1250 m from the discharge point a dilution index of 1:15 was measured which fits the expected value based on modeling.

Metal concentrations, fluxes, inventories and chronologies in sediments from Sepetiba and Ribeira Bays: a comparative study.

Three sediment cores were sampled at Sepetiba Bay and four cores at Ribeira Bay, Rio de Janeiro State, Brazil. Sediment accumulation rates and chronologies were obtained from (210)Pb activity-depth profiles. Sediment accumulation rates in Ribera Bay ranged from 1.2 mm y(-1) in the inner bay to 2.6 mm y(-1) close to its entrance. In Sepetiba Bay two sediment accumulation rates were observed: a lower rate of 0.35 cm y(-1) before the 1960s and 0.76 cm y(-1) since then. The cause of this change is due to the construction of the Santa Cecília impoundment (1955) that brings water from the Paraíba do Sul Basin into the Guandu River, which increased its flow from the original 20 m(3)s(-1) to 160 m(3)s(-1). Concentration of 44 elements was obtained by ICP-MS after total dissolution of samples from two selected cores. The relative differences between the concentrations of crustal elements, such as Al, Fe and Ti are only about 20% (p<0.05). Cd and Zn are 15 and four times larger in Sepetiba Bay than in Ribeira Bay, respectively. Other major and minor elements show lower statistically significant differences. The enrichment factors and metal inventories show that Sepetiba Bay can be considered polluted with Bi, Cd, Cr, Cu, Sb and Zn. Particularly, Cd and Zn present concentrations three and four times higher than the Brazilian existing limits.