Phytochemical content, antioxidant properties, and antibacterial activities of Centella asiatica L.

The present study is an evaluation of Centella asiatica ethanolic extract in terms of total phenolic content, total flavonoids, total flavonols, anthocyanin, antioxidants, and antibacterial activities. Centella asiatica plants were collected from Guilan province, Iran. High-performance liquid chromatography-Diode array detector analysis demonstrated that the ethanolic extract of C. asiatica contained phenolic substances such as sinapic acid, catechin, quercetin, p-coumaric acid, hesperidin, eugenol, and hesperetin. The predominant phenolic compound in the extract was hesperetin. The antibacterial activities of the extract were highly potent against S. aureus and B. cereus. These findings suggest that C. asiatica is rich in phenolic compounds and can be utilised for future cosmetic and pharmaceutical purposes.

Composition of the essential oil of different plant organs in Dorema ammoniacum D. Don and Dorema aucheri Boiss. accessions collected from Iran.

This study was performed to evaluate the chemical constituents of the essential oils (EOs) from the leaves and stems of different accessions of Dorema ammoniacum D. Don and Dorema aucheri Boiss. collected in Iran. Sesquiterpene hydrocarbons were identified as the major fractions in EOs of stem (65.58-87.48%) and leaves (59.41-84.33%) samples. ß-caryophyllene was identified as the major component in the EOs of leaves samples in all accessions of D. aucheri. Aromadandrene and tetradecane were identified as the major compound in stem and leaves samples of Dorema ammoniacum D. Don, respectively. The highest number of chemical constituents for leaves (27) and stem (28) samples were identified in D.au4 and D.au3 genotypes, respectively. The least number of chemical constituents for both leaves (18) and stem (19) samples were specified to D. ammoniacum species (D.am1 and D.am2). These results suggest the use of enriched-sesquiterpene hydrocarbons accessions of Dorema for future medicinal purposes.

Elicitation Effects on Some Secondary Metabolites and Antioxidant Activity in Callus Cultures of Allium jesdianum Boiss. & Buhse.: Methyl Jasmonate and Putrescine.

Allium jesdianum Boiss. & Buhse. is the most important species of the Amaryllidaceae family with various pharmacological properties. Three subsequent experiments (germination, callogenesis, and elicitation) were carried out as a completely randomized design with six replication. At the first study, the highest seed germination (78.33%) was achieved at chemical pre-treatment including the combination of α-naphthalene acetic acid (1 mg L-1) and benzylaminopurine (3 mg L-1) under in vitro condition. The highest callus induction (86.7%) was observed at MS/2 media, which was supplemented by NAA (1 mg L-1) and BAP (3 mg L-1) from hypocotyl explants. Then, two chemical elicitors including methyl jasmonate (MeJ) (0, 25, 50, and 100 µM) and putrescine (Pu) (0, 0.5, and 1 mM) were used to investigate their effects on different biochemical traits under callus culture. The results showed the superiority of MeJ over Pu for increasing the secondary metabolites and antioxidant activity in calluses of Allium jesdianum, compared to the control. The highest contents for total phenolics (6.02 mg GAE g-1 FW), total flavonoids (0.52 mg QE g-1 FW), and total flavonols (0.39 mg QE g-1 FW) were observed under 50 µM of MeJ. Meanwhile, the highest value for anthocyanin (8.99 µ mol g-1 FW) was achieved at 25 µM of MeJ. The highest 2,2-diphenyl-1-picrylhydrazyl activities were observed at 50 and 100 µM of MeJ. Putrescine (0.5 mM) elicitation showed only superiority for callus growth rate (0.53 mm day-1). Enhancement of desired secondary metabolites at 50 µM MeJ could be suitable for future studies in biotechnological aspects of this medicinal plant.

Determination of phenolic compounds, antioxidant and anticancer activity of Chrozophora tinctoria accessions collected from different regions of Iran.

The present study was conducted to determine the total phenolics, flavonoids, flavonols, anthocyanin, and carotenoids as well as the antioxidant activity of 10 different Crozophora tinctoria accessions originated from Iran. The highest contents of total phenolics, flavonoids, flavonols, anthocyanin, and carotenoids were detected in C.t (2), C.t (10), C.t (10), C.t (9), and C.t (3) accessions, respectively. The highest antioxidant activities of the samples, evaluated using 1,1-diphenyl-2-picrylhydrazyl and phosphomolibdate assays, were detected in C.t (8) and C.t (3), respectively. The highest phenyl aminolyase activity was observed in C.t (4). Ten phenolic compounds, including quercetin, catechin, ellagic acid, rosmarinic acid, hesperetin, eugenol, hesperidin, carvacrol, thymol, and kaempferol were identified and quantified using high-performance liquid chromatography (HPLC) technique. The best anticancer activity against HeLa cell lines was observed at C.t (8) accession, originated from Qazvin Region. This study confirmed that the C. tinctoria accessions with rich phenolics compounds may be recommended as a novel source of natural antioxidants for future aims in medicinal fields. PRACTICAL APPLICATIONS: According to this findings there was high variation among phenolics compounds of C. tinctoria which is revealed by HPLC analysis. This study identified phenolic compounds with antioxidant and anticancer activity in Iranian accessions of C. tinctoria. With an increase in the rate of cancer disease, superior accessions of C. tinctoria may be recommended as a novel source of natural antioxidants for ethno pharmacological aims as cancer therapy, and also in nutraceutical fields.