Search | VHL Regional Portal

Analysis of the Expression of Regulator Genes in Kupffer Cells and Monocytes.

Nikitina, M P; Elchaninov, A V; Lokhonina, A V; Makarov, A V; Tagirova, M K; Grinberg, M V; Bolshakova, G B; Glinkina, V V; Goldshtein, D V; Fatkhudinov, T Kh.

Bull Exp Biol Med ; 168(4): 556-560, 2020 Feb.

Article in English | MEDLINE | ID: mdl-32157510

ABSTRACT

Differences in the gene expression profiles in resident macrophages (in particular, Kupffer cells) and monocytes were revealed. However, these differences in gene expression profiles do not allow considering resident liver macrophages as purely M2 macrophages and monocytes as purely M1 macrophages. At the same time, a significant number of the genes upregulated in Kupffer cells are associated with normal regulation of liver functions (Arg 1, Flt, iNOs, and Kng). In monocytes, the expression of genes Alox15, Alox12, Tlr2, Tlr4, Tlr7, and Tlr8 (typical functional genes of macrophages) was also upregulated in comparison with Kupffer cells.

Subject(s)

Cell Lineage/genetics , Kupffer Cells/metabolism , Liver/metabolism , Monocytes/metabolism , Animals , Arachidonate 12-Lipoxygenase/genetics , Arachidonate 12-Lipoxygenase/metabolism , Arachidonate 15-Lipoxygenase/genetics , Arachidonate 15-Lipoxygenase/metabolism , Arginase/genetics , Arginase/metabolism , Biomarkers/metabolism , Gene Expression , Immunophenotyping , Kupffer Cells/classification , Kupffer Cells/cytology , Liver/cytology , Male , Membrane Glycoproteins/genetics , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred BALB C , Monocytes/classification , Monocytes/cytology , Nitric Oxide Synthase Type II/genetics , Nitric Oxide Synthase Type II/metabolism , Toll-Like Receptor 2/genetics , Toll-Like Receptor 2/metabolism , Toll-Like Receptor 4/genetics , Toll-Like Receptor 4/metabolism , Toll-Like Receptor 7/genetics , Toll-Like Receptor 7/metabolism , Toll-Like Receptor 8/genetics , Toll-Like Receptor 8/metabolism , Vascular Endothelial Growth Factor Receptor-1/genetics , Vascular Endothelial Growth Factor Receptor-1/metabolism

ABSTRACT

Subject(s)

SEND TO:

SELECTION OF CITATIONS

SEARCH DETAIL