ABSTRACT
Ruxolitinib (RXL) is a Janus kinase inhibitor used for treating intermediate- or high-risk myelofibrosis. This study presents an electrode modified with electrochemically polymerized taurine on a carbon paste electrode via cyclic voltammetry (CV). The surface characterization of the poly(taurine)-CP electrode was evaluated by using electrochemical (electrochemical impedance spectroscopyâEIS, CV), morphological (scanning electron microscopeâSEM), and spectroscopic (Fourier-transform infrared spectroscopyâFT-IR) techniques. Under optimized conditions, RXL exhibited good linearity within the 0.01-1.0 µM concentration range, with a limit of detection (LOD) of 0.005 µM. The proposed electrochemical sensor demonstrated excellent selectivity, accuracy, precision, and repeatability. Furthermore, it effectively detected RXL in human urine and pharmaceutical samples.
Subject(s)
Carbon , Electrodes , Nitriles , Pyrazoles , Pyrimidines , Taurine , Humans , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Carbon/chemistry , Electrochemical Techniques , Janus Kinase Inhibitors/chemistry , Janus Kinase Inhibitors/pharmacology , Materials Testing , Molecular Structure , Nitriles/chemistry , Nitriles/pharmacology , Particle Size , Polymerization , Pyrazoles/chemistry , Pyrazoles/pharmacology , Pyrimidines/chemistry , Pyrimidines/pharmacology , Taurine/chemistry , Taurine/analogs & derivatives , Taurine/pharmacologyABSTRACT
In this reported work, an anodically pretreated boron-doped diamond (BDD) electrode was used for the inexpensive, simple and quick detection of a natural dye, lawsone. Lawsone had a well-defined, irreversible and diffusion-controlled oxidation peak at approximately +0.19 V in phosphate buffer solution (PBS, 0.1 M, pH 2.5) using cyclic voltammetry (CV). The oxidation peak heights of lawsone were significantly increased in PBS using the cationic surfactant cetyltrimethylammonium bromide (CTAB). Under optimized experimental conditions, the calibration curve was linear over a concentration range of 0.1-5.0 µM with detection limit of 0.029 µM in 0.1 M PBS (pH 2.5) containing 0.1 mM CTAB by using square-wave voltammetry (SWV). To evaluate the practical applicability of the BDD electrode, it was used for the quantification of lawsone in commercial henna, a natural dye made from the leaves of the henna plant.