Pesticides driven pollution in Kuwait: The first evidence of environmental exposure to pesticides in soils and human health risk assessment.

Soil pollution from pesticide residues is a key concern due to the high soil accumulation of pesticides and their human toxicity. Pesticide concentration of surface soil samples from the Sulaibiya agricultural field located in Kuwait was assessed in the present study. The study also investigated health risk assessment for both children and adults based on the residual concentrations. The average concentration of Æ©OCPs (sum of organochlorine pesticides) along the present study was 3062 pg/g. The residual concentration of Æ©OCPs was comparatively lower as compared to other locations around the world. Out of the 11 observed locations, A, B, and D locations indicated higher concentrations of Æ©OCPs. The results indicated that DDT showed higher concentrations 692.87 pg/g in soil samples as compared to the other pesticides. Cancer risks of OCP via ingestion, dermal contact and inhalation of soil particles suggested that all stations were in a safe zone. However, locations A, B and D were closer to the low-risk band. The distribution pattern for each form of organochlorine pesticides (OCP) was different in Sulaibiya, indicating the non-simultaneous use of different groups of OCPs in this region. Multivariate statistical analysis based on cluster analysis identified three classes, 1, 2 and 3 of pesticides, suggesting these are from the same sources. Principle component analysis (PCA) showed that soil physicochemical properties influence the pesticides in soil samples. The results provides the baseline data of pesticides in soils from Kuwait.

Genetic diversity and population structure of Haloxylon salicornicum moq. in Kuwait by ISSR markers.

Haloxylon salicornicum moq. Bunge ex Boiss (Rimth) is one of the native plants of Kuwait, extensively depleting through the anthropogenic activities. It is important to conserve Haloxylon community in Kuwait as it can tolerate extreme adverse conditions of drought and salinity to be potentially used in the desert and urban revegetation and greenery national programs. Therefore, a set of 16 inter simple sequence repeat (ISSR) markers were used to assess genetic diversity and population structure of 108 genotypes from six locations in Kuwait. The ISSR primers produced 195 unambiguous and reproducible bands out of which 167 bands were polymorphic (86.5%) with a mean PIC value of 0.31. The overall average values of Nei's gene diversity (h') and Shannon's diversity index (I) were 0.254 and 0.375, respectively. Results of AMOVA revealed high genetic variations within populations (77.8%) and low among populations (22%). The values of Fixation index (FST = 0.22; P = 0.0), Genetic differentiation (GST = 0.262; G'ST = 0.327; D = 0.335 and Gene flow (NM = 0.880) were indicative of heterozygous populations. The results of STRUCTURE and split decomposition analysis suggested that the Rimth accessions of Kuwait can be grouped into five and six subpopulations, respectively. Principal coordinate analysis (PCoA) grouped them into three clusters. The pairwise Nei's genetic distances (DS) among populations demonstrated a narrow range from 0.047 to 0.187 (Scale-0.0 to 1.0). The Mantel's test revealed a weak correlation (r2- 0.188; P-0.013) between the genetic distance and geographic distances. Our results suggest that the narrowly distributed Haloxylon community in Kuwait demonstrated a high genetic diversity within the populations however the overall population structure was weak.