Multimodal sensing and intuitive steering assistance improve navigation and mobility for people with impaired vision.

Globally, more than 250 million people have impaired vision and face challenges navigating outside their homes, affecting their independence, mental health, and physical health. Navigating unfamiliar routes is challenging for people with impaired vision because it may require avoiding obstacles, recognizing objects, and wayfinding indoors and outdoors. Existing approaches such as white canes, guide dogs, and electronic travel aids only tackle some of these challenges. Here, we present the Augmented Cane, a white cane with a comprehensive set of sensors and an intuitive feedback method to steer the user, which addresses navigation challenges and improves mobility for people with impaired vision. We compared the Augmented Cane with a white cane by having sighted and visually impaired participants complete navigation challenges while blindfolded: walking along hallways, avoiding obstacles, and following outdoor waypoints. Across all experiments, the Augmented Cane increased the walking speed for participants with impaired vision by 18 ± 7% and sighted participants by 35 ± 12% compared with a white cane. The increase in walking speed may be due to accurate steering assistance, reduced cognitive load, fewer contacts with the environment, and higher participant confidence. We also demonstrate advanced navigation capabilities of the Augmented Cane: indoor wayfinding, recognizing and steering the participant to a key object, and navigating a sequence of indoor and outdoor challenges. The open-source and low-cost design of the Augmented Cane provides a platform that may improve the mobility and quality of life of people with impaired vision.

Transgenic models for investigating the nervous system: Currently available neurofluorescent reporters and potential neuronal markers.

Recombinant DNA technologies have enabled the development of transgenic animal models for use in studying a myriad of diseases and biological states. By placing fluorescent reporters under the direct regulation of the promoter region of specific marker proteins, these models can localize and characterize very specific cell types. One important application of transgenic species is the study of the cytoarchitecture of the nervous system. Neurofluorescent reporters can be used to study the structural patterns of nerves in the central or peripheral nervous system in vivo, as well as phenomena involving embryologic or adult neurogenesis, injury, degeneration, and recovery. Furthermore, crucial molecular factors can also be screened via the transgenic approach, which may eventually play a major role in the development of therapeutic strategies against diseases like Alzheimer's or Parkinson's. This review describes currently available reporters and their uses in the literature as well as potential neural markers that can be leveraged to create additional, robust transgenic models for future studies.

Underground Azelaic Acid-Conferred Resistance to Pseudomonas syringae in Arabidopsis.

Local interactions between individual plant organs and diverse microorganisms can lead to whole plant immunity via the mobilization of defense signals. One such signal is the plastid lipid-derived oxylipin azelaic acid (AZA). Arabidopsis lacking AZI1 or EARLI1, related lipid transfer family proteins, exhibit reduced AZA transport among leaves and cannot mount systemic immunity. AZA has been detected in roots as well as leaves. Therefore, the present study addresses the effects on plants of AZA application to roots. AZA but not the structurally related suberic acid inhibits root growth when directly in contact with roots. Treatment of roots with AZA also induces resistance to Pseudomonas syringae in aerial tissues. These effects of AZA on root growth and disease resistance depend, at least partially, on AZI1 and EARLI1. AZI1 in roots localizes to plastids, similar to its known location in leaves. Interestingly, kinases previously shown to modify AZI1 in vitro, MPK3 and MPK6, are also needed for AZA-induced root-growth inhibition and aboveground immunity. Finally, deuterium-labeled AZA applied to the roots does not move to aerial tissues. Thus, AZA application to roots triggers systemic immunity through an AZI1/EARLI1/MPK3/MPK6-dependent pathway and AZA effects may involve one or more additional mobile signals.