Growth of Scenedesmus obliquus in anaerobically digested swine wastewater from different cleaning processes for pollutants removal and biomass production.

Anaerobically digested swine wastewater (ASW) purification by microalgae provides a promising strategy for nutrients recovery, biomass production and CO2 capture. However, the characteristics of ASW from different cleaning processes vary greatly. At present, the cultivation of microalgae in ASW from different manure cleaning processes is rarely investigated and compared. That may bring uncertainty for microalgae growth using different ASW in large-scale application. Thus, the ASW from three cleaning processes were tested for cultivating microalgae, including manure dry collection (I), water flushing (II) and water submerging processes (III). The characteristics of ASW from three manure cleaning processes varied greatly such as nutrient and heavy metals levels. High concentration of ammonia and copper in ASW significantly inhibited microalgae growth. Fortunately, the supply of high CO2 (10%) effectively alleviated negative influences, ensuring microalgal growth at low dilution ratio. The characteristics of three ASW resulted in significant differences in microalgae growth and biomass components. The maximal biomass production in optimal diluted ASW-I, II and III reached 1.46 g L-1, 2.19 g L-1 and 2.47 g L-1, respectively. The removal of organic compounds, ammonia and phosphorus by optimal microalgae growth in diluted ASW-I, II and III was 50.6%/94.2%/64.7%, 63.7%/82.3%/57.6% and 83.2%/91.7%/59.7%, respectively. The culture in diluted ASW-I, II and III obtained the highest lipids production of 12.1 mg L-1·d-1, 16.5 mg L-1·d-1 and 19.4 mg L-1·d-1, respectively. The analysis of lipids compositions revealed that the proportion of saturated fatty acids accounted for 36.4%, 32.4% and 27.9 % in optimal diluted ASW-I, II and III, as ideal raw materials for biodiesel production.

Growth of Scenedesmus obliquus on anaerobic soybean wastewater using different wasted organics for high biomass production and nutrients recycling.

The microalgae culture in mixing sewage with different characteristics may significantly improve biomass production and nutrients recycling efficiency. In this study, three waste organic wastewater including molasses, alcohol and glycerol wastewater were mixed with anaerobic soybean wastewater as mediums for microalgae culture. The optimal mixture of molasses, alcohol and glycerol wastewater was at an initial carbon-nitrogen ratio of 7:1, 5:1 and 10:1, improving biomass production by 60.4%, 31.3% and 68.7%, respectively. The removal efficiencies of organics, ammonia nitrogen and phosphorus at optimal mixture were 54.8-62.4%, 79.5-99.1% and 49.3-61.5%, and the removal rates increased by 340-630%, 27.5-66.3% and 36.3-70.2% compared to the blank culture. In addition, the culture in mixed wastewater increased lipids contrast by 0.7-1.3 times, while achieving higher saturation in fatty acids. The results suggested that microalgae culture using mixed wastewater was a strategy for high biomass production and nutrients recycling efficiency.

Anaerobic digestates grown oleaginous microalgae for pollutants removal and lipids production.

Anaerobic digestates were potential mediums for cultivating oleaginous microalgae, but their various components brought uncertainties for aglal growth and lipids production. In this study, three microalgae strains were tested to grow on four typical anaerobic digestates. The results showed that anaerobic food wastewater was an optimal medium for C. pyrenoidosa and S. obliquus culture (N. oleoabundanst cannot survive), achieving the highest biomass (2.15-2.32 g L-1) and lipids production (20.6-32.5 mg L-1·d-1). In contrast, three microalgae strains could grow suboptimally in anaerobic municipal (0.79-0.95 g L-1) and toilet (0.92-1.40 g L-1) wastewater, but showed poor performances in anaerobic swine wastewater. The growth of microalgae removed 40.9-63.4% of TOC, 83.7-96.3% of NH4+-N and 70.3-89.4% of TP in the three ADs. In addition, it was unfortunately found that the lipids content and saturation degree in fatty acids significantly decreased in ADs with sufficient nutrients. It suggests that some measures should be taken to balance biomass, lipids production and quality for cultivating microalgae in anaerobic digestates.

Growth of Chlorella pyrenoidosa on different septic tank effluents from rural areas for lipids production and pollutants removal.

Septic tank effluent from rural areas was an ideal medium for cultivating oleaginous microalgae. However, the characteristics of septic tank effluents varied greatly due to the different incoming wastewater, and bring uncertain risks for algal growth. In this study, an oleaginous microalgae was cultivated in septic effluents from different mixed wastewater. The results showed that the effluent from pure toilet wastewater was the best medium to achieve the highest biomass yield (1.68 g·L-1) and productivity (154.6 mg·L-1·d-1). In contrast, the discharge of kitchen or laundry wastewater reduced the biomass production by 50.5-79.1%. That caused much lower lipids production in effluents from mixed wastewater regardless of its high lipids content and saturation degree. The results suggest that the discharge of kitchen or laundry wastewater bring risks for biomass and lipids production, and should be separated from the toilet wastewater before entering into septic tank.

Nutrients recycling and biomass production from Chlorella pyrenoidosa culture using anaerobic food processing wastewater in a pilot-scale tubular photobioreactor.

Microalgae cultivation in anaerobic food wastewater was a feasible way for high biomass production and nutrients recycling. In this study, Chlorella pyrenoidosa culture on anaerobic food wastewater was processed outdoors using a pilot-scale tubular photobioreactor. The microalgae showed rapid growth in different seasons, achieving high biomass production of 1.83-2.10 g L-1 and specific growth rate of 0.73-1.59 d-1. The biological contamination and dissolved oxygen were controlled at suitable levels for algal growth in the tubular photobioreactor. Lipids content in harvested biomass was 8.1-15.3% of dried weight, and the analysis in fatty acids revealed high quality with long carbon chain length and high saturation. Additionally, algal growth achieved effective pollutants purification from wastewater, removing 42.3-53.8% of CODCr, 82.6-88.7% of TN and 59.7-67.6% of TP. This study gave a successful application for scaled-up microalgae culture in anaerobic food processing wastewater for biodiesel production and wastewater purification.

Optimization of algae mixotrophic culture for nutrients recycling and biomass/lipids production in anaerobically digested waste sludge by various organic acids addition.

Anaerobically digested waste sludge contains very high concentrations of ammonium and phosphate that are difficult to be purified using traditional processes. Mixotrophic culture of microalgae is a potential way to achieve ammonium and phosphate removal, while harvesting considerable biomass for biodiesel production. In this study, four typical volatile organic acids that could be potentially produced from sludge fermentation were tested for algal mixotrophic culture in anaerobically digested waste sludge. The results showed that the addition of propionate and isovaleric acid had no significant improvement on biomass production, and even inhibited algal growth at low concentration. Fortunately, the addition of acetic and n-butyric acid (initial C/N = 10) increased biomass production by1.9-2.4 times compared to the blank culture. Higher biomass production increased ammonium and orthophosphate removal to 88.3-97.1% and 80.4-93.0%, respectively. Moreover, the optimal addition of volatile organic acids enhanced lipids production by 3.9-6.3 times, while achieving higher saturation degree in biodiesels. The results suggest that adding these optimal volatile organic acids is suitable to enhance nutrients recycling and algal biodiesel production from anaerobically digested waste sludge.

Lipids production and nutrients recycling by microalgae mixotrophic culture in anaerobic digestate of sludge using wasted organics as carbon source.

Insufficient organics in anaerobic digestate of sludge limited algal mixotrophic culture and caused low lipids production. In this study, enhancing lipids production and pollutants removal by adding acidified starch wastewater was tested for Chlorella pyrenoidosa mixotrophic culture. The results showed that an optimal addition of acidified starch wastewater into anaerobic digestate of sludge (1:1, v/v) improved biomass and lipids production by 0.5-fold (to 2.59 g·L-1) and 3.2-fold (87.3 mg·L-1·d-1), respectively. The acidified starch wastewater addition also improved the quality of algal biodiesel with higher saturation (typically in C16:0 and C18:0). In addition, 62% of total organic carbon, 99% of ammonium and 95% of orthophosphate in mixed wastewater were effectively removed by microalgae. This study provides a promising way to improve biodiesel production and nutrients recovery from anaerobic digestate of sludge using waste carbon source.

Cultivation of Chlorella pyrenoidosa in anaerobic wastewater: The coupled effects of ammonium, temperature and pH conditions on lipids compositions.

Anaerobic wastewater potentially was an ideal medium for cultivating microalgae. The coupled effect of ammonium, temperature and pH on lipids accumulation was a core issue during algal culture using anaerobic wastewater. Therefore, their combined effects on Chlorella pyrenoidosa culture and lipids accumulation in anaerobic effluent were investigated. Free ammonia induced from the rising pH and temperature inhibited algal growth, but significantly promoted lipid accumulation. The highest lipids content reached 30.2% when pH rose to 8.3-8.5 (25 °C, ammonium 280 mg/L), which was 1.6-fold higher than that under neutral condition. Moreover, the percentage of unsaturated fatty acids (un-SFAs) increased to 74.8-77.9% at pH 8.3-8.5, whereas it was only 56.1-58.9% under neutral condition. The C18:2 and C18:3 dominated the un-SFAs increase at high pH, typically the percentage of C18:3 increased by 74.5-153.1%. This study provides a potential way for lipid accumulation in algal culture using anaerobic wastewater.

Enhanced lipid and biomass production using alcohol wastewater as carbon source for Chlorella pyrenoidosa cultivation in anaerobically digested starch wastewater in outdoors.

Alcohol wastewater (AW) as carbon source for enhancing Chlorella pyrenoidosa growth and lipid accumulation in anaerobically digested starch wastewater (ADSW) was performed in outdoor cultivation. The biomass and lipid production significantly increased while adding optimal amount of AW (AW/ADSW=1:15) during exponential phase. In comparison with blank ADSW culture, the optimal AW addition increased the biomass production, lipid content and productivity by 35.29%, 102.68% and 227.91%, respectively. However, AW addition caused severe bacterial contamination and the total bacterial increased by 4.62-fold. Simultaneously, the optimal consortia of microalgae/bacteria effectively removed nutrients from the wastewater, including 405.18±36.47mgCODCr/L/day, 49.15±5.54mgN/L/day and 6.72±1.24mgP/L/day.

Outdoor cultures of Chlorella pyrenoidosa in the effluent of anaerobically digested activated sludge: The effects of pH and free ammonia.

A freshwater algae Chlorella pyrenoidosa was cultured outdoors using anaerobically digested activated sludge effluent. The effects of pH variations were evaluated. The coupled pH variations and free ammonia toxicity significantly affected the algal growth, lipids accumulation and contamination control during every season. The free ammonia toxicity at high pH levels actually inhibited the algal growth. Compared to an optimal algal growth at a pH of 5.7-6.5, biomass productivity at a high pH of 8.3-8.8 was reduced by 67.15±6.98%, 54.39±6.42% and 83.63±5.71% in the spring, fall and summer, respectively. When the pH rose above 9.1-9.6, algae were unable to grow in the wastewater. However, high pH levels reduced contamination (e.g., bacteria and microalgae grazers) and triggered lipids accumulation in algal cells. These findings suggest that pH control strategies are essential for this type of algal wastewater system, where ammonia is the dominant nitrogen source.

Chlorella pyrenoidosa cultivation in outdoors using the diluted anaerobically digested activated sludge.

A freshwater green algae Chlorella pyrenoidosa (C. pyrenoidosa) was cultured in outdoors using the diluted anaerobically digested activated sludge (ADAS). The outdoors batch culture in every season showed that C. pyrenoidosa can grow normally under natural conditions in the diluted ADAS (STE/ADAS=1.5/1, 3/1 and 5/1, v/v). Seasonal changes of environmental conditions significantly affected biomass growth and nutrient removal. Optimal biomass growth and nutrient removal was achieved at STE/ADAS=1.5/1 during summer culture, harvesting a maximum biomass concentration of 1.97 ± 0.21 g/L, average biomass productivity of 291.52 ± 33.74 g/m(3)/day (maximum value of 573.10 ± 41.82) and average lipids productivity of 37.49 ± 5.26 g/m(3)/day (maximum value of 73.70 ± 9.75); simultaneously, the microalgae growth effectively removed nutrients from the wastewater, including 105.6 ± 17.1 mg CODCr/L/day, 36.8 ± 6.1mg N/L/day and 6.1 ± 1.1 mg P/L/day.

Continuous cultivation of Chlorella pyrenoidosa using anaerobic digested starch processing wastewater in the outdoors.

Microalgae cultivation using wastewater might be a suitable approach to support sustainable large-scale biomass production. Its compelling characteristics included the recycling of nutrients and water resources, reducing carbon emissions and harvesting available biomass. In outdoor batch and continuous cultures, Chlorella pyrenoidosa completely adapted to anaerobic digested starch processing wastewater and was the dominant microorganism in the photobioreactor. However, seasonal changes of environmental conditions significantly influenced biomass growth and lipid production. The long-term outdoor operation demonstrated that the biomass concentration and productivity in continuous operations at different hydraulic retention times (HRTs) can be successfully predicted using the kinetic growth parameters obtained from the batch culture. A moderate HRT (4days) in the summer provided the best microalgae and lipid production and achieved relatively high biomass concentrations of 1.29-1.62g/L, biomass productivities of 342.6±12.8mg/L/d and lipids productivities of 43.37±7.43mg/L/d.