Fabrication of Mie-resonant silicon nanoparticles using laser annealing for surface-enhanced fluorescence spectroscopy.

Silicon nanostructures with unique Mie resonances have garnered considerable attention in the field of nanophotonics. Here, we present a simple and efficient method for the fabrication of silicon (Si) nanoparticle substrates using continuous-wave (CW) laser annealing. The resulting silicon nanoparticles exhibit Mie resonances in the visible region, and their resonant wavelengths can be precisely controlled. Notably, laser-annealed silicon nanoparticle substrates show a 60-fold enhancement in fluorescence. This tunable and fluorescence-enhancing silicon nanoparticle platform has tremendous potential for highly sensitive fluorescence sensing and biomedical imaging applications.

In Vivo Intelligent Fluorescence Endo-Microscopy by Varifocal Meta-Device and Deep Learning.

Endo-microscopy is crucial for real-time 3D visualization of internal tissues and subcellular structures. Conventional methods rely on axial movement of optical components for precise focus adjustment, limiting miniaturization and complicating procedures. Meta-device, composed of artificial nanostructures, is an emerging optical flat device that can freely manipulate the phase and amplitude of light. Here, an intelligent fluorescence endo-microscope is developed based on varifocal meta-lens and deep learning (DL). The breakthrough enables in vivo 3D imaging of mouse brains, where varifocal meta-lens focal length adjusts through relative rotation angle. The system offers key advantages such as invariant magnification, a large field-of-view, and optical sectioning at a maximum focal length tuning range of ≈2 mm with 3 µm lateral resolution. Using a DL network, image acquisition time and system complexity are significantly reduced, and in vivo high-resolution brain images of detailed vessels and surrounding perivascular space are clearly observed within 0.1 s (≈50 times faster). The approach will benefit various surgical procedures, such as gastrointestinal biopsies, neural imaging, brain surgery, etc.

Meta-Lens Particle Image Velocimetry.

Fluid flow behavior is visualized through particle image velocimetry (PIV) for understanding and studying experimental fluid dynamics. However, traditional PIV methods require multiple cameras and conventional lens systems for image acquisition to resolve multi-dimensional velocity fields. In turn, it introduces complexity to the entire system. Meta-lenses are advanced flat optical devices composed of artificial nanoantenna arrays. It can manipulate the wavefront of light with the advantages of ultrathin, compact, and no spherical aberration. Meta-lenses offer novel functionalities and promise to replace traditional optical imaging systems. Here, a binocular meta-lens PIV technique is proposed, where a pair of GaN meta-lenses are fabricated on one substrate and integrated with a imaging sensor to form a compact binocular PIV system. The meta-lens weigh only 116 mg, much lighter than commercial lenses. The 3D velocity field can be obtained by the binocular disparity and particle image displacement information of fluid flow. The measurement error of vortex-ring diameter is ≈1.25% experimentally validates via a Reynolds-number (Re) 2000 vortex-ring. This work demonstrates a new development trend for the PIV technique for rejuvenating traditional flow diagnostic tools toward a more compact, easy-to-deploy technique. It enables further miniaturization and low-power systems for portable, field-use, and space-constrained PIV applications.

Dynamic measurement of an angular Goos-Hänchen shift at a surface plasmon resonance in liquid.

We developed a surface plasmon resonance (SPR)-enhanced angular Goos-Hänchen (GH) shift measurement system capable of tracking small refractive index changes with high sensitivity in a liquid environment. Our method can be performed in angular interrogation schemes, where we demonstrate a simple zero-finding algorithm to locate the SPR angle instead of the complicated data processing algorithms used in conventional sensors. We also propose a displacement interrogation scheme for dynamic measurement of small refractive index changes in the sample. The main advantage of our method is the controllability of the measured displacement by standard geometrical optics, allowing measurement sensitivity enhancement without the need to modify the sensor material.

Intelligent Phase Contrast Meta-Microscope System.

Phase contrast imaging techniques enable the visualization of disparities in the refractive index among various materials. However, these techniques usually come with a cost: the need for bulky, inflexible, and complicated configurations. Here, we propose and experimentally demonstrate an ultracompact meta-microscope, a novel imaging platform designed to accomplish both optical and digital phase contrast imaging. The optical phase contrast imaging system is composed of a pair of metalenses and an intermediate spiral phase metasurface located at the Fourier plane. The performance of the system in generating edge-enhanced images is validated by imaging a variety of human cells, including lung cell lines BEAS-2B, CLY1, and H1299 and other types. Additionally, we integrate the ResNet deep learning model into the meta-microscope to transform bright-field images into edge-enhanced images with high contrast accuracy. This technology promises to aid in the development of innovative miniature optical systems for biomedical and clinical applications.

Varifocal Metalenses: Harnessing Polarization-Dependent Superposition for Continuous Focal Length Control.

Traditional varifocal lenses are bulky and mechanically complex. Emerging active metalenses promise compactness and design flexibility but face issues like mechanical tuning reliability and nonlinear focal length tuning due to additional medium requirements. In this work, we propose a varifocal metalens design based on superimposing light intensity distributions from two orthogonal polarization states. This approach enables continuous and precise focal length control within the visible spectrum, while maintaining relatively high focusing efficiencies (∼41% in simulation and ∼28% in measurement) and quality. In experimental validation, the metalens exhibited flexible tunability, with the focal length continuously adjustable between two spatial positions upon variation of the incident polarization angle. The MTF results showed high contrast reproduction and sharp imaging, with a Strehl ratio of >0.7 for all polarization angles. With compactness, design flexibility, and high focusing quality, the proposed varifocal metalens holds potential for diverse applications, advancing adaptive and versatile optical devices.

High-Resolution Metalens Imaging with Sequential Artificial Intelligence Models.

An analysis of the optical response of a GaN-based metalens was conducted alongside the utilization of two sequential artificial intelligence (AI) models in addressing the occasional issues of blurriness and color cast in captured images. The optical loss of the metalens in the blue spectral range was found to have resulted in the color cast of images. Autoencoder and CodeFormer sequential models were employed in order to correct the color cast and reconstruct image details, respectively. Said sequential models successfully addressed the color cast and reconstructed details for all of the allocated face image categories. Subsequently, the CIE 1931 chromaticity diagrams and peak signal-to-noise ratio analysis provided numerical evidence of the AI models' effectiveness in image reconstruction. Furthermore, the AI models can still repair the image without blue information. Overall, the integration of metalens and artificial intelligence models marks a breakthrough in enhancing the performance of full-color metalens-based imaging systems.

Label-free visualization of photosynthetic microbial biofilms using mid-infrared photothermal and autofluorescence imaging.

The formation of photosynthetic microbial biofilms comprising multispecies biomolecules, such as extracellular polymeric substances (EPSs), and microbial cells play pivotal roles in maintaining or stimulating their biological functions. Although there are numerous studies on photosynthetic microbial biofilms, the spatial distribution of EPS components that are vital for microbial biofilm formation, such as exopolysaccharides and proteins, is not well understood. Visualization of photosynthetic microbial biofilms requires label-free methods, because labelling EPSs results in structural changes or aggregation. Raman spectroscopy is useful for label-free visualization of biofilm constituents based on chemical contrast. However, interference resulting from the bright autofluorescence of photosynthetic molecules and the low detection efficiency of Raman scattering make visualization a challenge. Herein, we visualized photosynthetic microbial biofilms in a label-free manner using a super-resolution optical infrared absorption imaging technique, called mid-infrared photothermal (MIP) microscopy. By leveraging the advantages of MIP microscopy, such as its sub-micrometer spatial resolution, autofluorescence-free features, and high detection sensitivity, the distribution of cyanobacteria and their extracellular polysaccharides in the biofilm matrix were successfully visualized. This showed that cyanobacterial cells were aligned along acidic/sulfated polysaccharides in the extracellular environment. Furthermore, spectroscopic analyses elucidated that during formation of biofilms, sulfated polysaccharides initially form linear structures followed by entrapment of cyanobacterial cells. The present study provides the foundation for further studies on the formation, structure, and biological functions of microbial biofilms.

Single-cell infrared vibrational analysis by optical trapping mid-infrared photothermal microscopy.

Single-cell analysis by means of vibrational spectroscopy combined with optical trapping is a reliable platform for unveiling cell-to-cell heterogeneities in vast populations. Although infrared (IR) vibrational spectroscopy provides rich molecular fingerprint information on biological samples in a label-free manner, its application with optical trapping has never been achieved due to weak gradient forces generated by the diffraction-limited focused IR beam and strong background of water absorption. Herein, we present single-cell IR vibrational analysis that incorporates mid-infrared photothermal (MIP) microscopy with optical trapping. Optically trapped single polymer particles and red blood cells (RBCs) in blood could be chemically identified owing to their IR vibrational fingerprints. This single-cell IR vibrational analysis further allowed us to probe the chemical heterogeneities of RBCs originating from the variation in the intracellular characteristics. Our demonstration paves the way for the IR vibrational analysis of single cells and chemical characterization in various fields.

High-sensitivity hyperspectral vibrational imaging of heart tissues by mid-infrared photothermal microscopy.

Visualizing the spatial distribution of chemical compositions in biological tissues is of great importance to study fundamental biological processes and origin of diseases. Raman microscopy, one of the label-free vibrational imaging techniques, has been employed for chemical characterization of tissues. However, the low sensitivity of Raman spectroscopy often requires a long acquisition time of Raman measurement or a high laser power, or both, which prevents one from investigating large-area tissues in a nondestructive manner. In this work, we demonstrated chemical imaging of heart tissues using mid-infrared photothermal (MIP) microscopy that simultaneously achieves the high sensitivity benefited from IR absorption of molecules and the high spatial resolution down to a few micrometers. We successfully visualized the distributions of different biomolecules, including proteins, phosphate-including proteins, and lipids/carbohydrates/amino acids. Further, we experimentally compared MIP microscopy with Raman microscopy to evaluate the sensitivity and photodamage to tissues. We proved that MIP microscopy is a highly sensitive technique for obtaining vibrational information of molecules in a broad fingerprint region, thereby it could be employed for biological and diagnostic applications, such as live-tissue imaging.

Highly Stable Polymer Coating on Silver Nanoparticles for Efficient Plasmonic Enhancement of Fluorescence.

Surface coating of plasmonic nanoparticles is of huge importance to suppress fluorescence quenching in plasmon-enhanced fluorescence sensing. Herein, a one-pot method for synthesizing polymer-coated silver nanoparticles was developed using a functional polymer conjugated with disulfide-containing anchoring groups. The disulfides played a crucial role in covalently bonding polymers to the surface of the silver nanoparticles. The covalent bond enabled the polymer layer to form a long-term stable coating on the silver nanoparticles. The polymer layer coated was adequately thin to efficiently achieve plasmonic enhancement of fluorescence and also thick enough to effectively suppress quenching of fluorescence, achieving a huge net enhancement of fluorescence. The polymer-coated plasmonic nanoparticles are a promising platform for demonstrating highly sensitive biosensing for medical diagnostics.

Molecular Monolayer Sensing Using Surface Plasmon Resonance and Angular Goos-Hänchen Shift.

We demonstrate potential molecular monolayer detection using measurements of surface plasmon resonance (SPR) and angular Goos-Hänchen (GH) shift. Here, the molecular monolayer of interest is a benzenethiol self-assembled monolayer (BT-SAM) adsorbed on a gold (Au) substrate. Excitation of surface plasmons enhanced the GH shift which was dominated by angular GH shift because we focused the incident beam to a small beam waist making spatial GH shift negligible. For measurements in ambient, the presence of BT-SAM on a Au substrate induces hydrophobicity which decreases the likelihood of contamination on the surface allowing for molecular monolayer sensing. This is in contrast to the hydrophilic nature of a clean Au surface that is highly susceptible to contamination. Since our measurements were made in ambient, larger SPR angle than the expected value was measured due to the contamination in the Au substrate. In contrast, the SPR angle was smaller when BT-SAM coated the Au substrate due to the minimization of contaminants brought about by Au surface modification. Detection of the molecular monolayer acounts for the small change in the SPR angle from the expected value.

Varifocal Metalens for Optical Sectioning Fluorescence Microscopy.

Fluorescence microscopy with optical sectioning capabilities is extensively utilized in biological research to obtain three-dimensional structural images of volumetric samples. Tunable lenses have been applied in microscopy for axial scanning to acquire multiplane images. However, images acquired by conventional tunable lenses suffer from spherical aberration and distortions. Here, we design, fabricate, and implement a dielectric Moiré metalens for fluorescence imaging. The Moiré metalens consists of two complementary phase metasurfaces, with variable focal length, ranging from ∼10 to ∼125 mm at 532 nm by tuning mutual angles. In addition, a telecentric configuration using the Moiré metalens is designed for high-contrast multiplane fluorescence imaging. The performance of our system is evaluated by optically sectioned images obtained from HiLo illumination of fluorescently labeled beads, as well as ex vivo mice intestine tissue samples. The compact design of the varifocal metalens may find important applications in fluorescence microscopy and endoscopy for clinical purposes.

Metamaterial perfect absorber simulations for intensifying the thermal gradient across a thermoelectric device.

The thermal gradient across a thermoelectric device is the key to convert heat energy into electricity. Here, we propose a metamaterial perfect absorber (MPA) that increases the thermal gradient across a thermoelectric device by local heat generation through absorbing thermal radiation emitted from an infinite-size blackbody radiator. The MPA, when attached on top of a bismuth telluride thermoelectric device, generates local heat that propagates to the device, resulting in an additional thermal gradient. The amount of local heat generated at the MPA and the output power of the thermoelectric device loaded with the MPA are examined through numerical calculations.

Three-dimensional nanoprinting via charged aerosol jets.

Three-dimensional (3D) printing1-9 has revolutionized manufacturing processes for electronics10-12, optics13-15, energy16,17, robotics18, bioengineering19-21 and sensing22. Downscaling 3D printing23 will enable applications that take advantage of the properties of micro- and nanostructures24,25. However, existing techniques for 3D nanoprinting of metals require a polymer-metal mixture, metallic salts or rheological inks, limiting the choice of material and the purity of the resulting structures. Aerosol lithography has previously been used to assemble arrays of high-purity 3D metal nanostructures on a prepatterned substrate26,27, but in limited geometries26-30. Here we introduce a technique for direct 3D printing of arrays of metal nanostructures with flexible geometry and feature sizes down to hundreds of nanometres, using various materials. The printing process occurs in a dry atmosphere, without the need for polymers or inks. Instead, ions and charged aerosol particles are directed onto a dielectric mask containing an array of holes that floats over a biased silicon substrate. The ions accumulate around each hole, generating electrostatic lenses that focus the charged aerosol particles into nanoscale jets. These jets are guided by converged electric-field lines that form under the hole-containing mask, which acts similarly to the nozzle of a conventional 3D printer, enabling 3D printing of aerosol particles onto the silicon substrate. By moving the substrate during printing, we successfully print various 3D structures, including helices, overhanging nanopillars, rings and letters. In addition, to demonstrate the potential applications of our technique, we printed an array of vertical split-ring resonator structures. In combination with other 3D-printing methods, we expect our 3D-nanoprinting technique to enable substantial advances in nanofabrication.

Transient transmission of THz metamaterial antennas by impact ionization in a silicon substrate.

The picosecond dynamics of excited charge carriers in the silicon substrate of THz metamaterial antennas was studied at different wavelengths. Time-resolved THz pump-THz probe spectroscopy was performed with light from a tunable free electron laser in the 9.3-16.7 THz frequency range using fluences of 2-12 J/m2. Depending on the excitation wavelength with respect to the resonance center, transient transmission increase, decrease, or a combination of both was observed. The transient transmission changes can be explained by local electric field enhancement, which induces impact ionization in the silicon substrate, increasing the local number of charge carriers by several orders of magnitude, and their subsequent diffusion and recombination. The studied metamaterials can be integrated with common semiconductor devices and can potentially be used in sensing applications and THz energy harvesting.

Angular Goos-Hänchen Shift Sensor Using a Gold Film Enhanced by Surface Plasmon Resonance.

We demonstrate the surface plasmon resonance (SPR)-enhanced angular Goos-Hänchen (GH) shift. Typical SPR-enhanced GH shift measurements make use of loosely collimated beams, which enhances only the spatial GH shift (ΔGH). Unlike this scheme, we focused the incident beam to a small beam waist to induce enhancement in the angular GH shift (ΘGH). Although this makes ΔGH negligible, the enhancement of ΘGH is much larger than the decrease in ΔGH. In order to excite surface plasmons, we employ a Kretschmann configuration using a simple gold (Au) film on a substrate. We show that although the efficiency of surface plasmon excitation is decreased by the focused geometry, a significantly large ΘGH was induced. With the simultaneous measurement of reflectivity for SPR and the beam shift for the GH shift used in this work, we experimentally show the potential of measuring enhanced ΘGH toward sensing application when the Au film is exposed to local environmental changes even in the simplest thin film structure.

Multiscale Hierarchical Micro/Nanostructures Created by Femtosecond Laser Ablation in Liquids for Polarization-Dependent Broadband Antireflection.

In this work, we present the possibility of producing multiscale hierarchical micro/nanostructures by the femtosecond laser ablation of transition metals (i.e., Ta and W) in water and investigate their polarization-dependent reflectance. The hierarchical micro/nanostructures are composed of microscale-grooved, mountain-like and pit-rich structures decorated with hybrid laser-induced periodic surface structures (LIPSSs). The hybrid LIPSSs consist of low/high and ultrahigh spatial frequency LIPSSs (LSFLs/HSFLs and UHSFLs). LSFLs/HSFLs of 400-600 nm in a period are typically oriented perpendicular to the direction of the laser polarization, while UHSFLs (widths: 10-20 nm and periods: 30-50 nm) are oriented perpendicular to the curvatures of LSFLs/HSFLs. On the microstructures with height gradients, the orientations of LSFLs/HSFLs are misaligned by 18°. On the ablated W metasurface, two kinds of UHSFLs are observed. UHSFLs become parallel nanowires in the deep troughs of LSFLs/HSFLs but result in being very chaotic in shallow LSFLs, turning into polygonal nanonetworks. In contrast, chaotic USFLs are not found on the ablated Ta metasurfaces. With the help of Fourier transform infrared spectroscopy, it is found that microgrooves show an obvious polarization-dependent reflectance at wavelengths of 15 and 17.5 µm associated with the direction of the groove, and the integration of microstructures with LSFs/HSFLs/UHSFLs is thus beneficial for enhancing the light absorbance and light trapping in the near-to-mid-infrared (NIR-MIR) range.

Whitish daytime radiative cooling using diffuse reflection of non-resonant silica nanoshells.

Daytime radiative cooling offers efficient passive cooling of objects by tailoring their spectral responses, holding great promise for green photonics applications. A specular reflector has been utilized in cooling devices to minimize sunlight absorption, but such a glaring surface is visually less appealing, thus undesirable for public use. Here, by exploiting strong diffuse reflection of silica nanoshells in a polymer matrix, daytime radiative cooling below the ambient temperature is experimentally demonstrated, while showing whitish color under sunlight. The cooling device consists of a poly(methyl methacrylate) layer with randomly distributed silica nanoshells and a polydimethylsiloxane (PDMS) layer on an Ag mirror. The non-resonant nanoshells exhibit uniform diffuse reflection over the solar spectrum, while fully transparent for a selective thermal radiation from the underneath PDMS layer. In the temperature measurement under the sunlight irradiation, the device shows 2.3 °C cooler than the ambient, which is comparable to or even better than the conventional device without the nanoshells. Our approach provides a simple yet powerful nanophotonic structure for realizing a scalable and practical daytime radiative cooling device without a glaring reflective surface.

3D conical helix metamaterial-based isotropic broadband perfect light absorber.

We present the design and fabrication of an isotropic broadband perfect light absorber in the near-infrared range using 3D metamaterials with a single resonator in the unit cell. The metamaterial resonator is comprised of a gold conical helix supported on a silicon pillar with back reflector realized on a silicon substrate. Simulations and experiments have demonstrated that the proposed absorber achieves a broad absorption band of more than 3 µm in the 1.5-4.5 µm wavelength range with an average absorbance of more than 90%. The numerical and experimental analyses show that the proposed device can provide both incident angle and polarization independent operations, which further widens the application prospects of our device.