The influence of written medication reminder on patient experience among older adult patients: a repeat cross-sectional study.

BACKGROUND: Older adult patients are particularly vulnerable to medication-related issues during the discharge process. To enhance medication discharge education and patient experience, a written medication reminder, incorporating crucial medication side effects and warning signs, was implemented in medicine wards. This study aimed to examine the influence of this reminder on patient experience and medication-taking behaviors among older adults in public healthcare settings. METHODS: Two separate rounds of cross-sectional surveys were conducted before and after the program implementation among different discharged patients in each round. The study enrolled older adult patients aged ≥ 65 or their caregivers discharged from the medical wards of four pilot public hospitals in Hong Kong. A structured questionnaire was administered via telephone within 14 days of the patient's discharge. The survey assessed patients' experience with the provided medication information during discharge, including the clarity, adequacy, and usefulness of the information, as well as their overall experience with inpatient services. The self-reported medication-taken behaviors, including adherence and side-effect encounters, were also measured. RESULTS: A total of 1,265 responses were collected before the implementation of the medication reminder, and 1,426 responses were obtained after the implementation. Pre/post-implementation survey comparison showed significant improvement in patient experience regarding the clarity of the provided medication information (7.93 ± 1.84 vs. 8.18 ± 1.69, P = 0.002), adequacy (7.92 ± 1.93 vs. 8.15 ± 1.76, P = 0.014), and usefulness (8.06 ± 1.80 vs. 8.26 ± 1.70, P = 0.017), significantly positive experience on the overall discharge information (ß coefficient, 0.43 [95%CI, 0.30 to 0.56]) and inpatient service (ß coefficient, 0.47 [95%CI, 0.32 to 0.61]). In addition, the side effects encounters were significantly lower in the post-implementation survey group (11.6% vs. 9.0%, P = 0.04) and no statistical difference was found in self-reported medication adherence between the two groups. CONCLUSIONS: The provision of written medication reminders on key medication risks effectively improved older adult patients' experience and reduced side effects without any unintended negative consequences. The findings can serve as a reference for similar settings seeking to enhance post-discharge care among older adult patients. Future studies could investigate the influence in other specialties and age groups and include clinical outcomes to test the program's effectiveness.

Implementing the information system for older adult patients post-discharge self-management: a qualitative study.

BACKGROUND: Discharging older adult patients from the hospital poses risks due to their vulnerable conditions, complex instructions and limited health literacy. Insufficient information about medication side effects adds to patient concerns. To address this, a post-discharge information summary system was developed. While it has shown positive impacts, concerns exist regarding implementation fidelity. OBJECTIVE: This study employed a theory-driven approach to understand health providers' perspectives on effective implementation. METHOD: Individual semi-structured interviews were conducted via telephone with nurses, doctors and pharmacists from local public hospitals. All interviews were audio-recorded and transcribed verbatim. Theoretical Domains Framework (TDF) was applied for direct content analysis. Belief statements were generated by thematic synthesis under each of the TDF domains. RESULTS: A total of 98 participants were interviewed. Out of the 49 belief statements covering eight TDF domains, 19 were determined to be highly relevant to the implementation of the post-discharge information summary system. These TDF domains include knowledge, skills, social/professional role and identity, beliefs about consequences, intentions, memory, attention and decision processes, environmental context and resources and social influences. CONCLUSION: Our study contributes to the understanding of determinants in implementing discharge interventions for older adult patients' self-care. Our findings can inform tailored strategies for frontline staff, including aligning programme rationale with stakeholders, promoting staff engagement through co-creation, reinforcing positive programme outcomes and creating default settings. Future research should employ rigorous quantitative designs to examine the actual impact and relationships among these determinants.

Out-of-pocket expenditure, need, utilisation, and private health insurance in the Australian healthcare system.

Despite widespread public service provision, public funding, and private health insurance (PHI), 20% of all healthcare expenditure across the OECD is covered by out-of-pocket expenditure (OOPE). This creates an equity concern for the increasing number of individuals with chronic conditions and greater need, particularly if higher need coincides with lower income. Theoretically, individuals may mitigate OOPE risk by purchasing PHI, replacing variable OOPE with fixed expenditure on premiums. Furthermore, if PHI premiums are not risk-rated, PHI may redistribute some of the financial burden from less healthy PHI holders that have greater need to healthier PHI holders that have less need. We investigate if the burden of OOPE for individuals with greater need increases less strongly for individuals with PHI in the Australian healthcare system. The Australian healthcare system provides public health insurance with full, partial, or limited coverage, depending on the healthcare service used, and no risk rating of PHI premiums. Using data from the Household, Income and Labour Dynamics in Australia survey we find that individuals with PHI spend a greater share of their disposable income on OOPE and that the difference in OOPE share between PHI and non-PHI holders increases with greater need and utilisation, contrary to the prediction that PHI may mitigate OOPE. We also show that OOPE is a greater concern for poorer individuals for whom the difference in OOPE by PHI is the greatest.

Significant inter-annual fluctuation in CO2 and CH4 diffusive fluxes from subtropical aquaculture ponds: Implications for climate change and carbon emission evaluations.

Aquaculture ponds are potential hotspots for carbon cycling and emission of greenhouse gases (GHGs) like CO2 and CH4, but they are often poorly assessed in the global GHG budget. This study determined the temporal variations of CO2 and CH4 concentrations and diffusive fluxes and their environmental drivers in coastal aquaculture ponds in southeastern China over a five-year period (2017-2021). The findings indicated that CH4 flux from aquaculture ponds fluctuated markedly year-to-year, and CO2 flux varied between positive and negative between years. The coefficient of inter-annual variation of CO2 and CH4 diffusive fluxes was 168% and 127%, respectively, highlighting the importance of long-term observations to improve GHG assessment from aquaculture ponds. In addition to chlorophyll-a and dissolved oxygen as the common environmental drivers, CO2 was further regulated by total dissolved phosphorus and CH4 by dissolved organic carbon. Feed conversion ratio correlated positively with both CO2 and CH4 concentrations and fluxes, showing that unconsumed feeds fueled microbial GHG production. A linear regression based on binned (averaged) monthly CO2 diffusive flux data, calculated from CO2 concentrations, can be used to estimate CH4 diffusive flux with a fair degree of confidence (r2 = 0.66; p < 0.001). This algorithm provides a simple and practical way to assess the total carbon diffusive flux from aquaculture ponds. Overall, this study provides new insights into mitigating the carbon footprint of aquaculture production and assessing the impact of aquaculture ponds on the regional and global scales.

Community assembly of comammox Nitrospira in coastal wetlands across southeastern China.

Complete ammonia oxidizers (comammox Nitrospira) are ubiquitous in coastal wetland sediments and play an important role in nitrification. Our study examined the impact of habitat modifications on comammox Nitrospira communities in coastal wetland sediments across tropical and subtropical regions of southeastern China. Samples were collected from 21 coastal wetlands in five provinces where native mudflats were invaded by Spartina alterniflora and subsequently converted to aquaculture ponds. The results showed that comammox Nitrospira abundances were mainly influenced by sediment grain size rather than by habitat modifications. Compared to S. alterniflora marshes and native mudflats, aquaculture pond sediments had lower comammox Nitrospira diversity, lower clade A.1 abundance, and higher clade A.2 abundance. Sulfate concentration was the most important factor controlling the diversity of comammox Nitrospira. The response of comammox Nitrospira community to habitat change varied significantly by location, and environmental variables accounted for only 11.2% of the variations in community structure across all sites. In all three habitat types, dispersal limitation largely controlled the comammox Nitrospira community assembly process, indicating the stochastic nature of these sediment communities in coastal wetlands. IMPORTANCE Comammox Nitrospira have recently gained attention for their potential role in nitrification and nitrous oxide (N2O) emissions in soil and sediment. However, their distribution and assembly in impacted coastal wetland are poorly understood, particularly on a large spatial scale. Our study provides novel evidence that the effects of habitat modification on comammox Nitrospira communities are dependent on the location of the wetland. We also found that the assembly of comammox Nitrospira communities in coastal wetlands was mainly governed by stochastic processes. Nevertheless, sediment grain size and sulfate concentration were identified as key variables affecting comammox Nitrospira abundance and diversity in coastal sediments. These findings are significant as they advance our understanding of the environmental adaptation of comammox Nitrospira and how future landscape modifications may impact their abundance and diversity in coastal wetlands.

Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori.

The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid copepod, Tigriopus japonicus. Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 × 104 cells/mL-1). Each microbe's concentration was adjusted at 108 CFU/mL-1 and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods' survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the copepod's culture performance compared to control. B. subtilis was the most effective in decreasing the copepod's external and internal Vibrio loading. The probiotic concentrations in the copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics for mass copepod culture as live food for mariculture purposes.

Measurements of elemental iodine in soy sauces in Taiwan using a modified microplate method.

Background: Soy sauce is widely used in a variety of Asian dishes to enhance flavor. Soybean and most soybean products, including soy sauces, are listed as prohibited foods in a low iodine diet. However, the iodine content in soy sauces is largely unknown. The aim of this study was to determine the iodine content in domestic soy sauces in Taiwan. Methods: Twenty-five different kinds of soy sauces were diluted with distilled water and with a dilution factor of fifty or above. Iodine concentrations of the diluted samples were measured colourimetrically based on the Sandell-Kolthoff reaction by a modified microplate method. All the measurements were repeated twelve times on three different days for determination of mean and standard deviation (SD), and coefficients of variance (CV). Serial dilution and recovery tests were also performed for validation. The results were confirmed by an inductively coupled plasma mass spectrometry (ICP-MS) method. Results: Among the twenty-five surveyed soy sauces, most of them (n=22) were iodine-free (<16 ug/L, and thus un-detectable). The iodine concentrations (mean ± SD) of the three iodine-containing soy sauces were 2.7 ± 0.1, 5.1 ± 0.2, and 10.8 ± 0.6 mg/L, respectively. The inter-assay, intra-assay and total CVs were all <5.3% for the modified microplate method. The results obtained by ICP-MS were consistent with those of the modified microplate method. The recovery rates in the serial dilution test and recovery test ranged from 94.7% to 118.6%. Two of the three iodine-containing soy sauces were supplemented with kelp extract, while the other one without kelp extract had the highest amount of salt among the three iodine-containing soy sauces. Therefore, we postulate that iodized salt instead of kelp extract is the source of higher iodine content in that sauce. Conclusion: The results suggest that most soy sauces are iodine-free and may be allowed during low iodine diets.

Effects of landscape modification on coastal sediment nitrogen availability, microbial functional gene abundances and N2O production potential across the tropical-subtropical gradient.

Wetland sediment is an important nitrogen pool and a source of the greenhouse gas nitrous oxide (N2O). Modification of coastal wetland landscape due to plant invasion and aquaculture activities may drastically change this N pool and the related dynamics of N2O. This study measured the sediment properties, N2O production and relevant functional gene abundances in 21 coastal wetlands across five provinces along the tropical-subtropical gradient in China, which all had experienced the same sequence of habitat transformation from native mudflats (MFs) to invasive Spartina alterniflora marshes (SAs) and subsequently to aquaculture ponds (APs). Our results showed that change from MFs to SAs increased the availability of NH4+-N and NO3--N and the abundance of functional genes related to N2O production (amoA, nirK, nosZ Ⅰ, and nosZ Ⅱ), whereas conversion of SAs to APs resulted in the opposite changes. Invasion of MFs by S. alterniflora increased N2O production potential by 127.9%, whereas converting SAs to APs decreased it by 30.4%. Based on structural equation modelling, nitrogen substrate availability and abundance of ammonia oxidizers were the key factors driving the change in sediment N2O production potential in these wetlands. This study revealed the main effect patterns of habitat modification on sediment biogeochemistry and N2O production across a broad geographical and climate gradient. These findings will help large-scale mapping and assessing landscape change effects on sediment properties and greenhouse gas emissions along the coast.

Analytical validation of an inductively coupled plasma mass spectrometry method for urinary iodine concentration measurements in Taiwan.

BACKGROUND: Urinary iodine concentration (UIC) measured by Sandell-Kolthoff spectrophotometric method has been used in the Nutrition and Health Surveys in Taiwan but this method is time consuming and produces toxic waste from arsenic trioxide. The aim of this study was to develop and validate an inductively coupled plasma mass spectrometry (ICP-MS) system to determine UIC in Taiwan. METHODS: Samples and iodine calibrators were diluted 100-fold into an aqueous solution containing Triton X-100, 0.5% ammonia solution, and tellurium (128Te) as an internal standard. Digestion prior to analysis was not necessary. Precision, accuracy, serial dilution, and recovery tests were performed. A total of 1243 urine samples covering a wide range of iodine concentrations were measured by both Sandell-Kolthoff method and ICP-MS. Passing-Bablok regression and Bland-Altman plots were used to compare values across methods. RESULTS: The limit for detection and quantification by ICP-MS was 0.95 µg/L and 2.85 µg/L, respectively. The intra-assay and inter-assay coefficients were <10%, with a recovery range of 95%-105%. The results obtained by ICP-MS and the Sandell-Kolthoff method were highly correlated (Pearson's correlation: r = 0.996, 95% confidence interval [CI]: 0.9950-0.9961, p < 0.001). For UIC between 20 and 1000 µg/L, the y-intercept for the Passing-Bablok regression was -1.9 (95% CI: -2.5599 to -1.3500) and the slope was 1.01 (95% CI: 1.0000-1.0206). CONCLUSION: This validated ICP-MS system can be used for measuring UIC.

Effects of conversion of coastal marshes to aquaculture ponds on sediment anaerobic CO2 production and emission in a subtropical estuary of China.

The extensive conversion of carbon-rich coastal wetland to aquaculture ponds in the Asian Pacific region has caused significant changes to the sediment properties and carbon cycling. Using field sampling and incubation experiments, the sediment anaerobic CO2 production and CO2 emission flux were compared between a brackish marsh and the nearby constructed aquaculture ponds in the Min River Estuary in southeastern China over a three-year period. Marsh sediment had a higher total carbon and lower C:N ratio than aquaculture pond sediment, suggesting the importance of marsh vegetation in supplying labile organic carbon to the sediment. Conversion to aquaculture ponds significantly decreased sediment anaerobic CO2 production rates by 69.2% compared to the brackish marsh, but increased CO2 emission, turning the CO2 sink (-490.8 ± 42.0 mg m-2 h-1 in brackish marsh) into a source (6.2 ± 3.9 mg m-2 h-1 in aquaculture pond). Clipping the marsh vegetation resulted in the highest CO2 emission flux (382.6 ± 46.7 mg m-2 h-1), highlighting the critical role of marsh vegetation in capturing and sequestering carbon. Sediment anaerobic CO2 production and CO2 uptake (in brackish marsh) and emission (in aquaculture ponds) were highest in the summer, followed by autumn, spring and winter. Redundancy analysis and structural equation modeling showed that the changes of sediment temperature, salinity and total carbon content accounted for more than 50% of the variance in CO2 production and emission. Overall, the results indicate that vegetation clearing was the main cause of change in CO2 production and emission in the land conversion, and marsh replantation should be a primary strategy to mitigate the climate impact of the aquaculture sector.

Spillover effects of retirement: Does health vulnerability matter?

The current literature investigating the impact of retirement and the associated spousal spillover effects overlooks the unintended effects of retirement on spouses in vulnerable health, namely spouses with long-term health conditions (LTHCs). In this paper, we fill this gap in the literature and investigate the impact of an individual's retirement on their partner's health outcomes when their partner has LTHCs. Given the inherent identification challenges associated with entry into retirement, we use the pension-qualifying age in Australia as an instrument. Based on data from the Household Income and Labour Dynamics in Australia survey, we find that the husband's retirement has a positive impact on the wife's quality-adjusted life years (QALY) and other physical and mental health outcomes. We also identify redistribution of domestic workload as a key transmission mechanism of the spousal spillover effects. Women with LTHCs will see their QALY and health improve only if their husband devotes more time to domestic tasks after retirement.

Conversion of coastal wetland to aquaculture ponds decreased N2O emission: Evidence from a multi-year field study.

Land reclamation is a major threat to the world's coastal wetlands, and it may influence the biogeochemical cycling of nitrogen in coastal regions. Conversion of coastal marshes into aquaculture ponds is common in the Asian Pacific region, but its impacts on the production and emission of nitrogen greenhouse gases remain poorly understood. In this study, we compared N2O emission from a brackish marsh and converted shrimp aquaculture ponds in the Shanyutan wetland, the Min River Estuary in Southeast China over a three-year period. We also measured sediment and porewater properties, relevant functional gene abundance, sediment N2O production potential and denitrification potential in the two habitats. Results indicated that the pond sediment had lower N-substrate availability, lower ammonia oxidation (AOA and comammox Nitrospira amoA), nitrite reduction (nirK and nirS) and nitrous oxide reduction (nosZ Ⅰ and nosZ Ⅱ) gene abundance and lower N2O production and denitrification potentials than in marsh sediments. Consequently, N2O emission fluxes from the aquaculture ponds (range 5.4-251.8 µg m-2 h-1) were significantly lower than those from the marsh (12.6-570.7 µg m-2 h-1). Overall, our results show that conversion from marsh to shrimp aquaculture ponds in the Shanyutan wetland may have diminished nutrient input from the catchment, impacted the N-cycling microbial community and lowered N2O production capacity of the sediment, leading to lower N2O emissions. Better post-harvesting management of pond water and sediment may further mitigate N2O emissions caused by the aquaculture operation.

Differential serum interferon-ß levels in autoimmune thyroid diseases.

Introduction: Interferon (IFN)-ß is known as an environmental trigger for the occurrence of autoimmune thyroid disease (AITD). However, the association of another type-1 IFN, IFN-ß, with AITD is unknown. Material and methods: In the study, we explored the association of serum IFN-ß levels with AITD in an ethnic Chinese (i.e., Taiwanese) population. We enrolled 160 patients with Graves' disease (GD), 47 patients with Hashimoto's thyroiditis (HT), and 119 healthy controls. Serum IFN-ß and B-cell activating factor (BAFF) levels were quantified in healthy controls at the baseline and in patients with AITD either prior to receiving medication or while under medication. Thyroid function and thyroid-stimulating hormone receptor antibody (TSHRAb) levels were measured at the time of serum collection. Results: Serum IFN-ß levels were lower in the HT group than in the control group (p = 0.031). A significant inverse correlation was observed between IFN-ß and TSHRAb levels in men with GD (r = -0.433, p = 0.044). Serum IFN-ß levels were also negatively associated with BAFF levels in men with GD, HT, and AITD (r = -0.320, p = 0.032; r = -0.817, p = 0.047; and r = -0.354, p = 0.011, respectively), but not in women with GD, HT, or AITD. Conclusions: Serum IFN-ß levels were lower in HT patients. Correlations of serum IFN-ß with TSHRAb and BAFF levels were found to be gender-specific. Further well-designed studies with larger sample sizes are required to confirm our findings.

Large increase in CH4 emission following conversion of coastal marsh to aquaculture ponds caused by changing gas transport pathways.

Methane emissions from aquatic ecosystems play an important role in global carbon cycle and climate change. Reclamation of coastal wetlands for aquaculture use has been shown to have opposite effects on sediment CH4 production potential and CH4 emission flux, but the underlying mechanism remained unclear. In this study, we compared sediment properties, CH4 production potential, emission flux, and CH4 transport pathways between a brackish marsh and the nearby reclaimed aquaculture ponds in the Min River Estuary in southeastern China. Despite that the sediment CH4 production potential in the ponds was significantly lower than the marsh, CH4 emission flux in the ponds (17.4 ± 2.7 mg m-2 h-1) was 11.9 times higher than the marsh (1.3 ±  0.2 mg m-2 h-1). Plant-mediated transport accounted for 75% of the total CH4 emission in the marsh, whereas ebullition accounted for 95% of the total CH4 emission in the ponds. CH4 emission fluxes in both habitat types were highest in the summer. These results suggest that the increase in CH4 emission following the conversion of brackish marsh to aquaculture ponds was not caused by increased sediment CH4 production, but rather by eliminating rhizospheric oxidation and shifting the major transport pathway to ebullition, allowing sediment CH4 to bypass oxidative loss. This study improves our understanding of the impacts of modification of coastal wetlands on greenhouse gas dynamics.

Changes in sediment methanogenic archaea community structure and methane production potential following conversion of coastal marsh to aquaculture ponds.

Widespread conversion of coastal wetlands into aquaculture ponds in coastal region often results in degradation of the wetland ecosystems, but its effects on sediment's potential to produce greenhouse gases remain unclear. Using field sampling, incubation experiments and molecular analysis, we studied the sediment CH4 production potential and the relevant microbial communities in a brackish marsh and the nearby aquaculture ponds in the Min River Estuary in southeastern China. Sediment CH4 production potential was higher in the summer and autumn months than in spring and winter months, and it was significantly correlated with sediment carbon content among all environmental variables. The mean sediment CH4 production potential in the aquaculture ponds (20.1 ng g-1 d-1) was significantly lower than that in the marsh (45.2 ng g-1 d-1). While Methanobacterium dominated in both habitats (41-59%), the overall composition of sediment methanogenic archaea communities differed significantly between the two habitats (p < 0.05) and methanogenic archaea alpha diversity was lower in the aquaculture ponds (p < 0.01). Network analysis revealed that interactions between sediment methanogenic archaea were much weaker in the ponds than in the marsh. Overall, these findings suggest that conversion of marsh land to aquaculture ponds significantly altered the sediment methanogenic archaea community structure and diversity and lowered the sediment's capacity to produce CH4.

Investigating the epibiotic peritrich Zoothamnium intermedium Precht, 1935: Seasonality and distribution of its relationships with copepods in Chesapeake Bay (USA).

Zoothamnium intermedium is an obligate epibiont ciliate and has been found in a diverse array of hosts and environments. Different studies have reported conflicting distribution patterns and host preferences, even though studies in Chesapeake Bay have suggested that the ciliate has a strong host specificity for two calanoid copepod species. We examined the life cycle, host preferences, and ecological conditions conducive to Z. intermedium presence on copepods in Chesapeake Bay, the largest estuary in North America. The York River tributary was sampled biweekly from fall 2014 through summer 2015 for plankton, peritrichs and bacteria in the water column. Bacterial abundance in the water column peaked in fall and late spring, coinciding with increased abundance and species richness of non-epibiont peritrichs. Among the plankton, only the calanoid copepods Acartia tonsa and Centropages hamatus were colonized by Z. intermedium. The peritrich epibiont displayed higher colonization rates on C. hamatus even when A. tonsa was far more abundant. Multivariate correlation analysis of infestation prevalence on A. tonsa showed a strong correlation with dissolved oxygen, salinity and water temperature. Such correlations, along with differences in host species biology, might be driving the seasonality of this epibiotic relationship.

Interactions between multi-walled carbon nanotubes and plankton as detected by Raman spectroscopy.

Raman spectroscopy has been commonly used in materials science to detect chemicals. Based on inelastic scattering of light after incident photons interact with a molecule, it has high potential for non-destructive detection of specific contaminants in living biological specimens. The increasing use of carbon nanotubes (CNTs) increases its chance to enter the aquatic habitats through direct discharge, surface runoff and atmospheric deposition, but their potential environmental impacts remain poorly known. We tested the use of Raman spectroscopy to investigate the interactions between multi-walled CNTs (MWCNTs) and aquatic plankton in vivo. For phytoplankton cells (Scenedesmus obliquus) that were exposed to MWCNTs, Raman spectroscopy was able to distinguish between background biological material and MWCNTs that adhere to the cells (G-band peak at 1590 cm-1 and D-band peak at 1350 cm-1 in the Raman spectra that were unique to MWCNTs). Harmful effects of MWCNT exposure manifested as lower photosynthetic efficiency and/or lower specific growth rate in the phytoplankton. MWCNT particles also adhered to the body surface of zooplankton, especially the carapace. Both Ceriodaphnia sp. and Daphnia sp. ingested MWCNTs directly, which was verified by the signature G-band and D-band Raman peaks in the zooplankton gut region. MWCNTs remained in the gut overnight after the zooplankton had been returned to clean water, showing that the zooplankton retained MWCNTs inside their body for an extended time, thereby increasing the chance to disperse and transfer the contaminants throughout the aquatic food web. Our results demonstrate that Raman spectroscopy is a promising method for non-destructive investigation of the uptake and dynamic fate of CNTs and other contaminants in aquatic organisms.

Environmental drivers of nitrous oxide emission factor for a coastal reservoir and its catchment areas in southeastern China.

While Asia is projected to be one of the major nitrous oxide (N2O) sources in the coming decades, a more accurate assessment of N2O budget has been hampered by low data resolution and poorly constrained emission factor (EF). Since urbanized coastal reservoirs receive high nitrogen loads from diverse sources across a heterogeneous landscape, the use of a single fixed EF may lead to large errors in N2O assessment. In this study, we conducted high spatial resolution sampling of dissolved N2O, nitrate-nitrogen (NO3--N) and other physico-chemical properties of surface water in Wenwusha Reservoir and other types of water bodies (river, drainage channels, and aquaculture ponds) in its catchment areas in southeastern China between November 2018 and June 2019. The empirically derived EF (calculated as N2O-N:NO3--N) for the reservoir showed considerable spatial variations, with a 10-fold difference ranging from 0.8 × 10-3 to 8.8 × 10-3. The average EF varied significantly among the four types of water bodies in the following descending order: aquaculture ponds > river > drainage channels > reservoir. Across all the water bodies, the mean EF in summer was 1.8-3.5 and 1.7-2.8 fold higher than that in autumn and spring, respectively, owing to the elevated water temperature. Overall, our derived EF deviated considerably from the IPCC default value, which implied that the use of default EF could result in over- or under-estimation of N2O emissions by up to 42%. We developed a multiple regression model that could explain 82% of the variance in EF based on water temperature and the ratio between dissolved organic carbon and nitrate-nitrogen (p < 0.001), which could be used to improve the estimate of EF for assessing N2O emission from coastal reservoirs and other similar environments.

The pathogenic role of IFN-α in thyroiditis mouse models.

AIM: Patients with chronic hepatitis C are frequently treated with interferon (IFN)-α. Autoimmune thyroid disease occurs in 20% ~ 40% of IFN-α-treated patients. In this study, the effects of IFN-α administration on triggering and regulating autoimmune thyroiditis in various animal models were evaluated. MAIN METHODS: Exogenous IFN-α was given to naive CBA mice, and both thyroglobulin (TG) immunization-induced (CBA) and spontaneous autoimmune thyroiditis (NOD·H-2 h4) models. Thyroid function, and anti-thyroglobulin antibody (ATA) and B-cell-activating factor (BAFF) levels were measured. Alterations in transcriptome profiles were analyzed. KEY FINDINGS: In the TG-induced thyroiditis model, IFN-α administration reduced plasma free thyroxine levels but did not alter ATA titers, BAFF levels, or the severity of histological changes. Interestingly, even without changes in thyroid functions, four of eight mice in the IFN-α alone group exhibited thyroiditis compared to the control group. Immunologically, mice in the IFN-α group exhibited profound CD3+ cell infiltration in the thyroid and higher plasma BAFF levels compared to the control group. Meanwhile, pathological and serological alterations after IFN-α administration were not observed in the NOD·H-2 h4 model. An RNA sequencing analysis revealed that immunoregulatory signatures were not excited by IFN-α treatment in naive CBA mice. Meanwhile, innate and adaptive immunity, inflammatory cytokine, chemokine, and cell-killing signaling pathways were all stimulated by IFN-α administration after TG immunization of CBA mice. SIGNIFICANCE: We confirmed the remarkable effects of IFN-α in both initiating thyroid immunity and modulating thyroid function and immunoregulatory signatures in established autoimmune thyroiditis. We suggest that IFN-α should be administered with caution in clinical settings.

Possible interplay between estrogen and the BAFF may modify thyroid activity in Graves' disease.

A link between sex hormones and B-cell activating factor (BAFF), a crucial immunoregulator of autoimmune thyroid disease (AITD), may exist. The study aimed to elucidate the role of estrogen (E2) in regulating BAFF in Graves' disease (GD). In clinical samples, serum BAFF levels were higher in women than in men in both the GD and control groups. serum BAFF levels were associated with thyroid-stimulating hormone receptor antibody levels and thyroid function only in women and not in men. BAFF transcripts in peripheral blood mononuclear cells were higher in women with GD than those in the control group. Among GD patients with the AA genotype of rs2893321, women had higher BAFF transcripts and protein levels than men. In the progression of a spontaneous autoimmune thyroiditis (SAT) murine model, NOD.H-2h4, serum free thyroxine and BAFF levels were higher in female than in male mice. Moreover, exogenous E2 treatment increased serum BAFF levels in male SAT mice. Meanwhile, female SAT mice exhibited higher thyroid BAFF transcripts levels than either the E2-treated or untreated male SAT mouse groups. Our results showed that E2 might be implicated in modulating BAFF expression, and support a possible mechanism for the higher incidence of AITD in women.