ABSTRACT
Present data about hormonal regulation of haemostasis are often contradictory and are mostly based on clinical observations. The aim of the current research is to study the effects of the hormones of hypothalamic-pituitary-thyroid (HPT) axis on plasma levels (i.e. on the synthesis and secretion) of vitamin K-dependent coagulation factors in rats. The study was carried out on 65 male Wistar rats, divided into five groups. The animals were injected subcutaneously (s.c.) once daily for three consecutive days as follows: the first group was injected with Thyrotropin releasing hormone (TRH), in a dose of 0.06 mg/kg b.w.; the second group by Thyroid stimulating hormone (TSH), with a dose of 1 MU/kg b.w., the third and the fourth group respectively with Liothyroninum (Triiodothyronin ? T3) and Levothyroxinum (Thyroxin ? T4) with a dose of 0.08 mg/kg b.w. each. The control group rats were injected with saline (the solvent of the hormones), following the same schedule and volume per kg b.w. The necessary quantity of blood was acquired by a cardiac puncture under ether narcosis, and antigen levels of plasma factors II, VII, IX and X (FII:Ag, FVII:Ag, FIX:Ag and FX:Ag) were determined by ELISA kits (Diagnostica Stago, France). TRH, TSH, T3 and T4 significantly decreased the plasma antigen levels of FII and FVII (p<0.001). TRH, T3 and TSH reduced significantly FIX:Ag level( p<0.001 for TRH and T3 and p<0.05 for TSH) while T4 did not exert significant changes ( p>0.05). FX:Ag level was also significantly reduced by TRH, T3 (p<0.001), TSH and T4 (p<0.01). Plasma levels of vitamin K-dependent coagulation factors FÐÐ:Ag, FVÐÐ:Ag, FÐÐ¥:Ag and FÐ¥:Ag are significantly reduced under the influence of the hormones of hypothalamic-pituitary-thyroid axis which signifies their decreased synthesis and secretion. T4 does not induce substantial changes in FIX:Ag plasma level.
Subject(s)
Blood Coagulation Factors/biosynthesis , Blood Coagulation Factors/metabolism , Hypothalamo-Hypophyseal System/metabolism , Protein Biosynthesis/drug effects , Thyroid Gland/metabolism , Vitamin K/metabolism , Animals , Hormones/metabolism , Hormones/pharmacology , Male , Rats , Rats, WistarABSTRACT
The modulation of learning and memory after left or right microinjections of the selective 5-HT1A receptor agonist 8-OH-DPAT and of the 5-HT1A receptor antagonist NAN190 into the hippocampal CA1 area of male Wistar rats was studied. Microinjections of 8-OH-DPAT (1 microg) into the right or left CA1 hippocampal area produced a significant decrease in the number of avoidances in a shuttle box. The impairing effect of 8-OH-DPAT was more pronounced when injected into the right hippocampus compared to the left one. Microinjections of NAN190 (1 microg) into the right or left CA1 hippocampal area produced a significant increase in the number of avoidances in a shuttle box. Right microinjections of NAN190 increased the number of avoidances more strongly than compared to left injections. These effects on learning and memory were more pronounced after injection of either of the serotonergic agents into the right CA1 hippocampal area compared to the left. The stronger memory-modulating effect after injection of 8-OH-DPAT or NAN190 into the right CA1 hippocampal area suggests a rightward bias in the rat.
Subject(s)
8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology , Functional Laterality/physiology , Hippocampus/anatomy & histology , Hippocampus/metabolism , Learning/physiology , Memory/physiology , Receptor, Serotonin, 5-HT1A/drug effects , Serotonin Receptor Agonists/pharmacology , Serotonin/metabolism , 8-Hydroxy-2-(di-n-propylamino)tetralin/administration & dosage , Animals , Avoidance Learning/drug effects , Hippocampus/surgery , Male , Rats , Rats, Wistar , Serotonin Receptor Agonists/administration & dosage , Stereotaxic TechniquesABSTRACT
The effects of somatostatin microinjected bilaterally and unilaterally (left or right) at a dose of 10, 50 and 100 ng into the caudate putamen of male Wistar rats on nociception (analgesy-meter test) were studied. Somatostatin injected into caudate putamen resulted in analgesia. Bilateral microinjections of somatostatin significantly increased the pain threshold in a dose-dependent manner, i.e. somatostatin exerted antinociceptive effect. The pain threshold after left-side microinjections was significantly higher than that after injections into right-side. These findings suggest antinociceptive and asymmetric effects of somatostatin on pain in the caudate putamen.
Subject(s)
Analgesics/pharmacology , Caudate Nucleus/drug effects , Somatostatin/administration & dosage , Somatostatin/pharmacology , Animals , Dose-Response Relationship, Drug , Male , Pain/drug therapy , Rats , Rats, WistarABSTRACT
The present study examined the behavioral responses to bilateral microinjections of somatostatin (SRIF) into caudate putamen of male Wistar rats. SRIF locally administered at doses of 10, 50 and 100 ng/side dose-dependently affected locomotor activity, as reflected in both horizontal and vertical movements. SRIF modulated locomotor activity in a biphasic manner, exerting an inhibitory and a facilitatory effect. In the elevated plus-maze experiments, SRIF at doses of 50 and 100 ng/side microinjected bilaterally into caudate putamen decreased only the total number of entries in the open and closed maze arms, confirming the suppressing effect of SRIF on locomotion at the first 5 min.
