Heterogeneous nucleation induced A. pernyi/B. mori silk fibroin coatings on AZ31 biometals with enhanced corrosion resistance, adhesion and biocompatibility.

Silk fibroin coatings on biomedical magnesium alloys have garnered significant attention due to their enhanced corrosion resistance and biocompatibility. However, the utilization of wild A. pernyi silk fibroin, known for its RGD sequence that facilitates tissue regeneration, presents a challenge for corrosion-resistant coatings on magnesium alloys due to its weak adhesion and high dissolution rate. In this study, we employed hexafluoroisopropanol as a solvent to blend A. pernyi silk fibroin with B. mori silk fibroin. The resulting blended fibroin coating at a 3:7 mass ratio exhibited a heterogeneous nucleation effect, enhancing ß-sheet content (32.3 %) and crystallinity (28.6 %). This improved ß-sheet promoted the "labyrinth effect" with an Icorr of 2.15 × 10-6 A cm-2, resulting in significantly improved corrosion resistance, which is two orders of magnitude lower than that of pure magnesium alloy. Meanwhile, the increased content of exposed serine in zigzag ß-sheet contributes to a higher adhesion strength. Cell cytotoxicity evaluation confirmed the enhanced cell adhesion and bioactivity. This work provides a facile approach for wild A. pernyi silk fibroin coatings on magnesium alloys with enhanced corrosion resistance, adhesion and biocompatibility.

Understanding the Interfacial Adhesion between Natural Silk and Polycaprolactone for Fabrication of Continuous Silk Biocomposites.

The poor interfacial adhesion between silk fiber and polyester species remains a critical problem for the optimal mechanical performance of silk-reinforced polyester composites. Here, we investigated in quantitative terms the interfacial properties between natural silk fibers and polycaprolactone (PCL) at nano-, micro-, and macroscales and fabricated continuous silk-PCL composite filaments by melt extrusion and drawing processing of PCL melt at 100, 120, and 140 °C. Bombyx mori (Bm) silk, Antheraea pernyi (Ap) silk, and polyamide6 (PA6) fiber were compared to the composite with PCL. The Ap silk exhibited the highest surface energy, the best wettability, and the largest interfacial shear strength (IFSS) with PCL. The silk-PCL composite from the 120 °C melt processing displayed the highest tensile modulus, implying an optimal temperature for interfacial adhesion. The Raman imaging technique revealed in detail the nature of the physical fusion of the interface phase in these silk- and polyamide-reinforced PCL composites. This work is intended to lay a foundation for the design and processing of robust composites from continuous silk fibers and bioresorbable polyesters for potential structural biomaterials.

Preparation and in vitro activity of single chain antibodies against Alzheimer's disease.

Disease-modifying passive immunotherapies focused on reducing amyloid-beta (Aß) deposition are a potential therapeutic strategy for Alzheimer's disease (AD). However, the results of Aß passive immunotherapy clinical trials were unsatisfactory, largely due to the low efficacy of whole antibodies due to their relatively large molecular weight and low blood-brain barrier transmittance. Furthermore, the constant region fragments of whole antibodies can trigger inflammatory reactions, which raises safety concerns. Single chain fragment variables (scFvs), containing only the variable region of the heavy and light chains of antibodies, show great potential for the treatment of AD. With the aim of generating a safe and effective AD passive immunotherapy, we designed and successfully prepared scFvs targeting Aß and investigated their activity in vitro. The results showed that both the 10D5-scFv and 12B4-scFv have high affinities for Aß monomers, oligomers, and fibers. Moreover, scFvs could prevent the formation of Aß oligomers and fibers, and block their cellular toxicity. In addition, 10D5-scFv and 12B4-scFv could bind to Aß plaques on the sections of mice brains in the in vitro study, indicating potential for the treatment of AD.

Theoretical and experimental investigation of the antioxidative activity of monascin.

Monascin, a specific type of monascus pigments, exhibits many bioactivities. In this study, the antioxidative activity of monascin was investigated by theoretical and experimental methods. First, the antioxidant potential of six monascus pigments was predicted by density functional theory (DFT) and time-dependent density functional theory (TDDFT) at the B3LYP/6-31+G (d, p) level, and monascus yellow pigments were predicted to have strong antioxidant capacity, as they can transfer hydrogen to free radicals and accept electrons from radicals. Then, the free radical-scavenging capacity of monascin for 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and hydroxyl radicals was experimentally validated by electron spin resonance (ESR) measurement. Monascin exhibited a quenching effect on DPPH, superoxide, and hydroxyl radicals in a dose-dependent manner. Specifically, the scavenging activity of monascin for DPPH, superoxide, and hydroxyl radicals was 97.5%, 59.5%, and 68.6%, respectively, when 0.1 mg mL-1 monascin was present. Our study provides theoretical evidence for the strong antioxidative activity of monascin and offers a simple and reliable strategy to determine the antioxidative activity.