Orthohantaviruses infections in humans and rodents in Baoji, China.

In recent years, hemorrhagic fever with renal syndrome (HFRS) incidence has been becoming a severe public health problem again due to its significant increase in Shaanxi Province, China. Baoji, located in the Guanzhong Plain in the central part of Shaanxi Province, has been severely affected by HFRS since its first emergence in 1955. To better understand the epidemiology of orthohantaviruses infection in humans and the causative agents carried by the rodents, the long-term incidence patterns were analyzed and a molecular epidemiological investigation of orthohantaviruses infection in humans and rodents was performed. During 1984-2019, 13,042 HFRS cases were registered in Baoji, including 275 death cases. Except the first high prevalence of HFRS in 1988-1993, another two epidemic peaks were observed in 1998-2003 and 2012, respectively, although vaccination project was started since 1996. During the same period, HFRS cases in Baoji mainly were recorded in winter suggesting they may be caused by Hantaan orthohantavirus (HTNV), while a small peak of HFRS was also found in summer with unknown reason. Nucleotide identity and phylogenetic analyses demonstrated that a novel clade of HTNV sequences recovered from HFRS cases were closely related to those from rodents, including species close contact with humans, suggesting a direct viral transmission from rodents to humans and the important role in the HTNV transmission the nontraditional rodent hosts may play. Moreover, two distant related Dabieshan orthohantavirus (DBSV) lineages were also identified in Niviventer niviventer in this area demonstrating its considerable genetic diversity. Our data indicated that continual spillover of HTNV from rodents to humans, contributing to the high prevalence of HFRS in humans in Baoji.

Extensive genetic diversity of Anaplasma bovis in ruminants in Xi'an, China.

Anaplasma bovis is an organism significant to cattle and buffalo since it is one of the causative agents of bovine anaplasmosis. Previous studies have shown the worldwide distribution of A. bovis. However, most of these studies about its genetic diversity only focused on the rrs gene. In this study, DNA of A. bovis was detected in blood samples of cattle and goats in Xi'an city, China by nested-PCR. Near full-length rrs, groEL, and gltA genes were amplified successfully from the positive samples. Genetic analysis showed that specific genetic marker (an insertion and a deletion) was found in the rrs sequences in some strains, as well as clone 88 from monkeys in previous study. Phylogenetic analysis based on the rrs, groEL, and gltA genes revealed that A. bovis circulating in Xi'an exhibited great genetic diversity. Our results also indicated that variants outside China presented geographic clustering, and all A. bovis isolates based on the groEL or gltA gene also showed a host origin clustering. Also of note was that the phylogenetic analyses of the groEL and gltA genes suggested that both frequent dispersals over long distances in recent years and local adaptation over long evolutionary timescales played important roles in the distribution and evolution of A. bovis in China. Finally, a potential recombination event in the genome of Zhouzhi-cattle-10 based on inconsistent positions in the groEL and gltA trees was also observed. These results also reinforce the need for assessing the pathogenicity to humans of A. bovis variants with specific marker in the rrs gene.