ABSTRACT
A phylogenetic analysis of samples taken from reported outbreaks of peste des petits ruminants virus (PPRV) in Georgia revealed a closer relationship to viruses from northern and eastern Africa than to viruses from countries closer to Georgia. This finding has crucial implications for the control of PPRV in the region.
Subject(s)
Disease Outbreaks , Goat Diseases/epidemiology , Peste-des-Petits-Ruminants/epidemiology , Peste-des-petits-ruminants virus/genetics , RNA, Viral/genetics , Sheep Diseases/epidemiology , Africa/epidemiology , Animals , Antigens, Viral/genetics , Georgia (Republic)/epidemiology , Goat Diseases/transmission , Goat Diseases/virology , Goats , Peste-des-Petits-Ruminants/transmission , Peste-des-Petits-Ruminants/virology , Peste-des-petits-ruminants virus/classification , Peste-des-petits-ruminants virus/isolation & purification , Phylogeny , Phylogeography , Sheep , Sheep Diseases/transmission , Sheep Diseases/virologyABSTRACT
Brucellosis is the one of most common livestock zoonoses in Georgia, resulting in significant economic losses. Livestock were sampled in three regions of Georgia (Kakheti, Kvemo Kartli, Imereti). Districts that historically reported high numbers of brucellosis related morbidity were selected for serological, bacteriological and molecular surveys. Surveying efforts yielded samples from 10,819 large and small ruminants. In total, 735 serological tests were positive on Rose Bengal and 33 bacterial isolates were recovered and identified as Brucella melitensis or Brucella abortus by microbiology and AMOS-PCR. A Bayesian framework was implemented to estimate the true prevalence of the disease given an imperfect diagnostic test. Regional posterior median true prevalence estimates ranged from 2.7% (95% CI: 1.4, 7.2) in Kvemo Kartli, 0.8% (95% CI: 0.0, 3.6) in Kakheti, to an estimate of 0.6% (95% CI: 0.0, 2.9) in Imereti. Accurate and efficient surveillance of brucellosis is not only of economic value, but also informs efforts to reduce the disease impact on the human population.