Pharmacokinetics of florfenicol and thiamphenicol after single oral and intravenous, as well as multiple oral administrations to geese.

1. This study evaluated the pharmacokinetic profiles of florfenicol (FF) and thiamphenicol (TP), which are synthetic bacteriostatic antimicrobial drugs, in geese after a single intravenous or oral administration, as well as seven oral doses administered at 12 h intervals. For all treatments, the dose was 30 mg/kg. 2. After single IV administration, clearance and volume of distribution were low (0.23 ± 0.03 l/h/kg and 0.57 ± 0.08 l/kg for FF, and 0.23 ± 0.04 l/h/kg and 0.59 ± 0.08 l/kg for TP, respectively). The elimination half-life was similar between products and short (2.91 ± 0.41 and 2.84 ± 0.64 h for FF and TP, respectively). 3. The single oral administration resulted in efficient absorption (bioavailability of 83.15 ± 11.48 for FF and 75.21 ± 19.56% for TP) with high maximal concentrations of 30.47 ± 2.47 and 20.02 ± 3.87 µg/ml for FF and TP, respectively. The area under the curve was 108.36 ± 14.96 and 101.81 ± 26.48 mg×h/l for FF and TP, respectively. 4. For both drugs, the two latter parameters were found to be higher compared to earlier studies on terrestrial birds. This suggested that FF and TP may be efficient in treating infections in geese caused by certain bacteria sensitive to chloramphenicol. 5. Neither drug accumulated in tissues following the oral seven doses and no adverse effects were noted in any treated animals. Thus, the selected FF and TP dosage may be considered as a safe treatment for geese.

Synthesis, Cytotoxicity Assessment and Optical Properties Characterization of Colloidal GdPO4:Mn2+, Eu3+ for High Sensitivity Luminescent Nanothermometers Operating in the Physiological Temperature Range.

Herein, a novel synthesis method of colloidal GdPO4:Mn2+,Eu3+ nanoparticles for luminescent nanothermometry is proposed. XRD, TEM, DLS, and zeta potential measurements confirmed the crystallographic purity and reproducible morphology of the obtained nanoparticles. The spectroscopic properties of GdPO4:Mn2+,Eu3+ with different amounts of Mn2+ and Eu3+ were analyzed in a physiological temperature range. It was found that GdPO4:1%Eu3+,10%Mn2+ nanoparticles revealed extraordinary performance for noncontact temperature sensing with relative sensitivity SR = 8.88%/°C at 32 °C. Furthermore, the biocompatibility and safety of GdPO4:15%Mn2+,1%Eu3+ was confirmed by cytotoxicity studies. These results indicated that colloidal GdPO4 doped with Mn2+ and Eu3+ is a very promising candidate as a luminescent nanothermometer for in vitro applications.

Efficient synthesis of PMMA@Co0.5Ni0.5Fe2O4 organic-inorganic hybrids containing hyamine 1622 - Physicochemical properties, cytotoxic assessment and antimicrobial activity.

The PMMA@Co0.5Ni0.5Fe2O4 ferrite containing hybrid nanomaterials with hyamine were prepared using emulsion polymerization method. Structural and morphological properties were evaluated using XRD, FT-IR, SEM techniques. The TGA and DTA analysis were performed in order to study the thermal properties of hybrid materials in contrast to reference material. Magnetic properties were studied using Quantum Design PPMS (VSM option) in a constant external magnetic field equal (100 Oe and 1000 Oe) in the temperature range from 2 to 380 K. Both the pure Co0.5Ni0.5Fe2O4and the sample with 85% of PMMA exhibit superparamagnetic behavior whereas blocking temperatureTB decreases with increase of PMMA content. The cytotoxicity assessment of PMMA@Co0.5Ni0.5Fe2O4 with hyamine in J774.E murine macrophages and U2OS human osteosarcoma cell lines was performed. Additionally, sensitivity of bacteria Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 25923 to hybrid materials (with/without hyamine) was investigated using a of Kirby-Bauer disc method.

[Working conditions and morbidity rate in employers at the South-East railway transport].

In the article there are presented the results of the examination of working conditions atfactories of the South-Eastern Railway, which has lost its effectiveness due to the action of certain laws. The situation on labor conditions was shown to be not entirely safe. There is given an analysis of the morbidity due to the temporary disability and occupational disease rates in foot-plate staff workers. There was made an emphasis on working conditions and morbidity rate in foot-plate staff workers. There is posed a question about the inclusion of essential hypertension in foot-plate staff workers in the list of occupational diseases.

High-performance liquid chromatographic investigation of the amino acid, amino sugar and neutral sugar content in glycoproteins.

A methods for the simultaneous separation and determination of amino acids, amino sugars and neutral carbohydrates is described. Stepwise elution systems with sodium citrate and borate buffers have developed for the ion-exchange liquid chromatographic separation of amino acids and sugars, using 8-micrometer particle size resins and the Stein and Moore and orcinol colorimetric method for detection. With the aid of this system, the direct quantitative comparison of sugars and amino acids by liquid chromatography becomes possible for the first time.