ABSTRACT
Systemic complement activation has been noted in a variety of shock states, and there is growing evidence that, in addition to being proinflammatory effectors, products of complement activation contribute directly to generalized manifestations of shock, such as hypotension and acidosis. To study the effects of complement activation, we examined responses in rats to systemic activation of complement with cobra venom factor (CVF), including blood pressure, metabolic acidosis, changes in vascular permeability, and lung function. High doses of CVF produced circulatory collapse (mean arterial pressure = 110 +/- 16 and 35 +/- 9 mmHg in control and with CVF, respectively, P < 0.05), metabolic acidosis (HCO concentration = 27.8 +/- 1.7 and 9.6 +/- 3.4 meq/l in control and with CVF, respectively, P < 0.05), extravasation of albumin into the lung and gut, and modest arterial hypoxemia (PO2 = 486 +/- 51 and 201 +/- 36 Torr in control and during 100% O2 breathing, respectively, P < 0.05). Prior depletion of complement protected against these abnormalities. Other interventions, including neutrophil depletion and cyclooxygenase inhibition, prevented lung injury but had much less effect on systemic hemodynamics or gut permeability, suggesting that complement activation products induce injury by neutrophil- and cyclooxygenase-dependent pathways in the lung but not in the gut. These studies underscore the significant systemic abnormalities developing after systemic activation of complement.
Subject(s)
Complement Activation/physiology , Hemodynamics/physiology , Lung/physiology , Acidosis/metabolism , Animals , Blood Pressure/physiology , Capillary Permeability/drug effects , Complement Inactivator Proteins/pharmacology , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Cyclooxygenase Inhibitors/pharmacology , Elapid Venoms/pharmacology , Enzyme Inhibitors/pharmacology , Hemodynamics/drug effects , Intestine, Small/pathology , Isoenzymes/metabolism , Lung/pathology , Lysine Carboxypeptidase/antagonists & inhibitors , Myocardium/pathology , Neutrophils/physiology , Oxygen/blood , Phospholipases A/antagonists & inhibitors , Prostaglandin-Endoperoxide Synthases/metabolism , Rats , Respiratory Function TestsABSTRACT
The complement system has been implicated in early inflammatory events and a variety of shock states. In rats, we measured complement activation after hemorrhage and examined the hemodynamic and metabolic effects of complement depletion before injury and worsening of complement activation after hemorrhage and resuscitation [with a carboxypeptidase N inhibitor (CPNI), which blocks the clearance of C5a]. Rats were bled to a mean arterial pressure of 30 mmHg for 50 min and were then resuscitated for 2 h. Shock resulted in significant evidence of complement consumption, with serum hemolytic activity being reduced by 33% (P < 0.05). Complement depletion before injury did not affect hemorrhage volume (complement depleted = 28 +/- 1 ml/kg, complement intact = 29 +/- 1 ml/kg, P = 0.74) but improved postresuscitation mean arterial pressure by 37 mmHg (P < 0.05) and serum bicarbonate levels (complement depleted = 22 +/- 3 meq/ml, complement intact = 13 +/- 8 meq/ml, P < 0.05). Pretreatment with CPNI was lethal in 80% of treated animals vs. the untreated hemorrhaged group in which no deaths occurred (P < 0.05). In this model of hemorrhagic shock, complement activation appeared to contribute to progressive hypotension and metabolic acidosis seen after resuscitation. The lethality of CPNI during acute blood loss suggests that the anaphylatoxins are important in the pathophysiological events involved in hemorrhagic shock.
Subject(s)
Complement Activation , Shock, Hemorrhagic/immunology , Acidosis/metabolism , Acidosis/physiopathology , Animals , Blood Pressure , Complement Activation/drug effects , Complement Inactivator Proteins/pharmacology , Elapid Venoms/pharmacology , Lysine Carboxypeptidase/antagonists & inhibitors , Male , Myocardial Ischemia/physiopathology , Myocardial Reperfusion , Rats , Rats, Sprague-Dawley , Resuscitation , Shock, Hemorrhagic/metabolism , Shock, Hemorrhagic/physiopathology , Survival RateABSTRACT
OBJECTIVES: The adhesion molecule L-selectin plays an important role in leukocyte-endothelium interactions, thereby contributing to inflammatory reactions. We tested the hypothesis that humanized anti-L-selectin antibodies reduce trauma-associated organ damage and mortality. DESIGN: Prospective, randomized experimental study. SETTING: Independent nonprofit research laboratory in a trauma hospital (Ludwig Boltzmann Institute) and a contract research institute (Biocon). SUBJECTS: Twenty-eight male baboons (Papio ursinus), 18 to 29 kg. INTERVENTIONS: Hemorrhagic-traumatic shock was created by complement activation with cobra venom factor, followed by withdrawal of blood to a mean arterial pressure of 35 to 45 mm Hg. Blood and lactated Ringer's solution were reinfused. Animals were randomized to receive either 2 mg/kg humanized anti-L-selectin antibody (HuDREG-55 [Ab]) or placebo (lactated Ringer's solution [LRS]). MEASUREMENTS AND MAIN RESULTS: Treatment with humanized anti-L-selectin antibody decreased mortality (Ab 21% vs. LRS 71%; p = .011) and improved survival time (p = .016). A trend toward reduced organ damage, especially in the adrenal glands (score 1.2 +/- 0.2 placebo vs. 1.0 +/- 0.1 antibody; p = .059) was seen, and at 24 hrs was accompanied by significantly increased mean arterial pressure (Ab 99 +/- 6 mm Hg vs. LRS 79 +/- 8 mm Hg; p = .023), cardiac output (Ab 3.4 +/- 0.2 L/min vs. LRS 2.4 +/- 0.3 L/min; p = .007), core temperature (p = .048), and improved perfusion, with less negative base excess (Ab 2.9 +/- 1.1 vs. LRS 2.1 +/- 1.7; p = .019) and a trend toward less lactate (p = .065). These improvements were accompanied by significantly (p = .006) decreased fluid requirements in the treatment group (Ab 11.7 +/- 2.5 mL/kg/hr vs. LRS 23.0 +/- 2.3 mL/kg/hr). There were also fewer circulating leukocytes (p = .042) in the treatment group at 24 hrs. CONCLUSION: Humanized anti-L-selectin antibody has beneficial effects on survival in a long-term in vivo model of hemorrhagic. traumatic shock.
Subject(s)
Antibodies, Monoclonal/therapeutic use , L-Selectin/immunology , Shock, Hemorrhagic/therapy , Shock, Traumatic/therapy , Animals , Bacterial Translocation/drug effects , Complement Activation/drug effects , Complement Inactivator Proteins , Elapid Venoms , Endothelium/immunology , Hemodynamics/drug effects , Leukocytes/drug effects , Male , Papio , Prospective Studies , Random Allocation , Shock, Hemorrhagic/immunology , Shock, Hemorrhagic/pathology , Shock, Traumatic/immunology , Shock, Traumatic/pathology , Survival Analysis , Systemic Inflammatory Response Syndrome/immunology , Systemic Inflammatory Response Syndrome/pathologyABSTRACT
Partial-thickness skin burns have been shown to induce neutrophil-dependent microvascular injury both locally (skin) and systemically (lung). In the present study, interventional measures to block inflammatory chemoattractants were employed to define the pathophysiologic role of these mediators in the development of secondary lung injury following thermal injury of skin. Rats were treated with blocking antibodies to either C5a or to the alpha-chemokines, keratinocyte-derived cytokine (KC), or macrophage inflammatory protein-2 (MIP-2). To study the role of platelet activating factor, a receptor antagonist (PAF-Ra) was utilized. The development of lung vascular injury following thermal injury to skin was significantly attenuated by treatment with anti-C5a (84%), anti-KC (67%), and anti-MIP-2 (77%), but treatment with PAF-Ra had no protective effects. Protective interventions were paralleled by significant reductions in the tissue buildup of myeloperoxidase. When bronchoalveolar lavage fluids from thermally injured rats were evaluated, elevations in TNF;ZA and IL-1 were found and were determined to be C5a-dependent (but unaffected by treatment with PAF-Ra). These studies indicate that lung tissue injury after thermal skin burns is dependent on chemotactic mediators. The data also suggest that lung expression of TNFalpha and IL-1 after thermal injury of skin is C5a-dependent.
Subject(s)
Burns/physiopathology , Chemotactic Factors/physiology , Chemotaxis, Leukocyte/physiology , Lung Diseases/physiopathology , Lung Injury , Neutrophils/physiology , Receptors, Cell Surface , Receptors, G-Protein-Coupled , Skin/injuries , Animals , Antibodies, Blocking/pharmacology , Bronchoalveolar Lavage Fluid/chemistry , Burns/enzymology , Capillary Permeability , Chemokine CXCL2 , Chemokines , Chemotactic Factors/immunology , Chemotaxis, Leukocyte/drug effects , Complement C5a/immunology , Complement C5a/physiology , Cytokines/immunology , Cytokines/physiology , Interleukin-1/metabolism , Lung/blood supply , Lung/enzymology , Lung/physiopathology , Lung Diseases/enzymology , Male , Monokines/immunology , Monokines/physiology , Neutrophils/drug effects , Peroxidase/metabolism , Platelet Activating Factor/immunology , Platelet Activating Factor/physiology , Platelet Membrane Glycoproteins/antagonists & inhibitors , Rabbits , Rats , Rats, Long-Evans , Tumor Necrosis Factor-alpha/metabolismABSTRACT
The effect of vitamin B2 (riboflavin) on oxidant-mediated acute lung injury has been examined in three different rat models. Pulmonary injury was induced by intravenous injection of cobra venom factor (CVF), by the intrapulmonary deposition of IgG immune complexes, or by hind limb ischemia-reperfusion. In each of the three models, injury was characterized by increases in vascular permeability (leakage of 125I-labeled bovine serum albumin), alveolar hemorrhage (extravasation of 51Cr-labeled rat erythrocytes), and neutrophil accumulation (myeloperoxidase activity). Intraperitoneal administration of riboflavin at a dose of 6 micromoles/kg body weight reduced vascular leakage by 56% in the CVF model, by 31% in the immune complex model, and by 53% in the lung injury model following ischemia-reperfusion of the hind limbs. Similar treatment reduced hemorrhage by 76%, 51%, and 70% in the three models of lung injury. In the CVF model, riboflavin was also shown to decrease products of lipid peroxidation (conjugated dienes) in lungs (by 45%) and in plasma (by 74%). Neutrophil accumulation in the lungs was not influenced by riboflavin administration in any of the three models. The studies demonstrate that riboflavin can mount a significant protection against oxidant-mediated inflammatory organ injury.
Subject(s)
Lung Diseases/prevention & control , Lung/physiopathology , Oxidative Stress/drug effects , Photosensitizing Agents/pharmacology , Reperfusion Injury/prevention & control , Riboflavin/pharmacology , Animals , Cattle , Complement Inactivator Proteins/toxicity , Elapid Venoms/toxicity , Lung/blood supply , Male , Oxidants/toxicity , Photosensitizing Agents/therapeutic use , Rats , Rats, Long-Evans , Riboflavin/therapeutic useABSTRACT
Acute thermal trauma is well known to produce evidence of a "systemic inflammatory response" in vivo, as manifested by evidence of complement activation, appearance in plasma of a variety of inflammatory factors, and development of multi-organ injury. The current studies were focused on acute thermal injury of rat skin and factors responsible for accompanying activation of blood neutrophils. Acute thermal injury of rat skin resulted in a time-dependent loss of L-selectin and up-regulation of Mac-1 (CD11b/CD18) on blood neutrophils, with no changes in LFA-1 (CD11a/CD18). The loss of L-selectin was prevented by blockade of C5a but not by blockade of the alpha-chemokine, macrophage inflammatory protein-2 (MIP-2). C5a, the alpha chemokines, MIP-2 and keratinocyte-derived cytokine (KC), and platelet activating factor (PAF) contributed to up-regulation of blood neutrophil Mac-1. Blocking interventions against these mediators also blunted the degree of neutropenia developing after thermal trauma. These data suggest that activation of blood neutrophils after thermal trauma is related to the role of several chemotactic mediators. These studies may provide clues regarding factors responsible for development of the "systemic inflammatory response syndrome" after thermal injury in the experimental model employed.
Subject(s)
Burns/immunology , Chemotactic Factors/physiology , Neutrophil Activation/immunology , Receptors, Cell Surface , Receptors, G-Protein-Coupled , Animals , Antibodies, Blocking/pharmacology , Burns/metabolism , Burns/therapy , CD11 Antigens/biosynthesis , CD18 Antigens/biosynthesis , Cell Adhesion Molecules/biosynthesis , Chemokine CXCL2 , Chemokines , Complement C5a/antagonists & inhibitors , Complement C5a/immunology , Complement C5a/physiology , Cytokines/antagonists & inhibitors , Cytokines/physiology , Immune Sera/pharmacology , L-Selectin/biosynthesis , Leukocyte Count , Male , Monokines/antagonists & inhibitors , Monokines/immunology , Monokines/physiology , Neutrophils/immunology , Neutrophils/metabolism , Platelet Membrane Glycoproteins/antagonists & inhibitors , Rats , Rats, Long-Evans , Up-Regulation/immunologyABSTRACT
The complement inhibitor soluble complement receptor type 1 (sCR1) and a truncated form of sCR1, sCR1[desLHR-A], have been generated with expression of the selectin-reactive oligosaccharide moiety, sialyl Lewisx (sLex), as N-linked oligosaccharide adducts. These modified proteins, sCR1sLex and sCR1[desLHR-A]sLex, were assessed in the L-selectin- and P-selectin-dependent rat model of lung injury following systemic activation of complement by cobra venom factor and in the L-selectin-, P-selectin-, and E-selectin-dependent model of lung injury following intrapulmonary deposition of IgG immune complexes. In the cobra venom factor model, sCR1sLex and sCR1[desLHR-A]sLex caused substantially greater reductions in neutrophil accumulation and in albumin extravasation in lung when compared with the non-sLex-decorated forms. In this model, increased lung vascular binding of sCR1sLex and sCR1[desLHR-A]sLex occurred in a P-selectin-dependent manner, in contrast to the absence of any increased binding of sCR1 or sCR1[desLHR-A]. In the IgG immune complex model, sCR1[desLHR-A]sLex possessed greater protective effects relative to sCR1[desLHR-A], based on albumin extravasation and neutrophil accumulation. Enhanced protective effects correlated with greater lung vascular binding of sCR1[desLHR-A]sLex as compared with the non-sLex-decorated form. In TNF-alpha-activated HUVEC, substantial in vitro binding occurred with sCR1[desLHR-A]sLex (but not with sCR1[desLHR-A]). This endothelial cell binding was blocked by anti-E-selectin but not by anti-P-selectin. These data suggest that sLex-decorated complement inhibitors have enhanced antiinflammatory effects and appear to have enhanced ability to localize to the activated vascular endothelium.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Complement Inactivator Proteins/therapeutic use , Endothelium, Vascular/immunology , Endothelium, Vascular/pathology , Lewis Blood Group Antigens/immunology , Lung/pathology , Oligosaccharides/immunology , Anti-Inflammatory Agents, Non-Steroidal/immunology , Complement Inactivator Proteins/genetics , Complement Inactivator Proteins/immunology , Elapid Venoms/administration & dosage , Endothelium, Vascular/metabolism , Humans , Immune Complex Diseases/immunology , Immune Complex Diseases/pathology , Immune Complex Diseases/therapy , Immunohistochemistry , Infusions, Intravenous , Lewis Blood Group Antigens/genetics , Lung/blood supply , Lung/chemistry , Lung/metabolism , Oligosaccharides/genetics , Oligosaccharides/therapeutic use , Protein Binding/immunology , Receptors, Complement 3b/genetics , Receptors, Complement 3b/therapeutic use , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/therapeutic use , Repetitive Sequences, Amino Acid , Sequence Deletion , Sequence Homology, Amino Acid , Sialyl Lewis X AntigenABSTRACT
In previous studies we have demonstrated that second-degree thermal injury of skin in rats leads to secondary effects, such as systemic complement activation, C5a-mediated activation of blood neutrophils, their adhesion-molecule-guided accumulation in lung capillaries and the development of acute pulmonary injury, largely caused by neutrophil-derived toxic oxygen metabolites. In the dermal burn wound, however, pathophysiologic events are less well understood. The injury is fully developed at four hours post-burn. To further elucidate the pathogenesis of the "late phase" dermal vascular damage, rats were depleted of neutrophils or complement by pretreatment with rabbit antibody against rat neutrophils or with cobra venom factor, respectively. In other experiments, rats were treated with blocking antibodies to IL-6, IL-1, and TNF alpha immediately following thermal burning or were pretreated with hydroxyl radical scavengers (dimethyl sulfoxide, dimethyl thiourea). Extravasation of 125I-labeled bovine serum albumin into the burned skin was studied, as well as, skin myeloperoxidase levels. The studies revealed that, like in secondary lung injury, neutrophils and toxic oxygen metabolites, are required for full development of microvascular injury. In contrast, however, development of dermal vascular damage in thermally injured rats was not affected by complement depletion. Our data suggest that the development of microvascular injury in the dermal burn wound is complement-independent, involves the pro-inflammatory cytokines IL-1, TNF alpha and IL-6, and may result from reactive oxygen metabolites generated by neutrophils accumulating in the burn wound.
Subject(s)
Burns/immunology , Capillaries/immunology , Capillaries/injuries , Complement Activation , Interleukin-1/immunology , Interleukin-6/immunology , Skin/blood supply , Skin/injuries , Tumor Necrosis Factor-alpha/immunology , Animals , Cattle , Male , Microcirculation/immunology , Neutrophil Activation , Rabbits , Rats , Wound Healing/immunologyABSTRACT
OBJECTIVE: This study evaluates the ability of perflubron to inhibit pulmonary neutrophil accumulation during partial liquid ventilation (PLV) in the setting of acute lung injury. DESIGN: Randomized, controlled, nonblinded study. SETTING: Research laboratory at a university. SUBJECTS: Male, Sprague-Dawley rats (n = 120, 506 +/- 42 g). INTERVENTIONS: Animals were divided into eight groups (n = 15 in each group, of which n = 12 for myeloperoxidase content and n = 3 for histologic neutrophil counting): a) GV-CVF group, animals received gas ventilation (GV) with the induction of lung injury using cobra venom factor (CVF); b) PLV-CVF group, animals received partial liquid ventilation before the induction of lung injury; c) PEEP-CVF group, animals received positive end-expiratory pressure (PEEP) before the administration of cobra venom factor; d) CVF-PLV group, animals received partial liquid ventilation after cobra venom factor; e) CVF-PEEP group, animals received PEEP after cobra venom factor; f) PLV only group, animals received partial liquid ventilation only; g) GV only group, animals received gas ventilation only; and h) NVSBA group, nonventilated spontaneous breathing animals. MEASUREMENTS AND MAIN RESULTS: After the experimental period, total lung myeloperoxidase content was significantly decreased in the PLV-CVF (0.29 +/- 0.08, p = .02) and PEEP-CVF (0.34 +/- 0.04, p = .01) groups when compared with the GV-CVF group (0.62 +/- 0.07). When compared with the GV-CVF group, a trend toward a reduction in myeloperoxidase was observed in the CVF-PLV (0.42 +/- 0.05, p = .07) and the CVF-PEEP (0.39 +/- 0.06, p = .07) groups. When compared with the cobra venom factor only group (GV-CVF 47 +/- 2 neutrophils/high-power field), reductions in neutrophil count were observed in all groups (neutrophils/high-power field): PLV-CVF (20 +/- 2, p = .009); PEEP-CVF (24 +/- 1, p = .01); CVF-PLV (30 +/- 2, p = .03); and CVF-PEEP (37 +/- 1, p = .04). CONCLUSION: These data suggest that both partial liquid ventilation and PEEP result in a reduction in neutrophil accumulation in the setting of acute lung injury.
Subject(s)
Fluorocarbons/therapeutic use , Neutrophils/immunology , Positive-Pressure Respiration/methods , Respiration, Artificial/methods , Respiratory Distress Syndrome/immunology , Respiratory Distress Syndrome/therapy , Animals , Blood Gas Analysis , Disease Models, Animal , Drug Evaluation, Preclinical , Elapid Venoms , Hydrocarbons, Brominated , Instillation, Drug , Leukocyte Count , Male , Peroxidase/analysis , Random Allocation , Rats , Rats, Sprague-Dawley , Respiratory Distress Syndrome/metabolism , Respiratory Distress Syndrome/pathologyABSTRACT
PURPOSE: This study evaluated the ability of partial liquid ventilation (PLV, gas ventilation of the perfluorocarbon-filled lungs) to reduce the amount of lung albumin leak present in the setting of acute lung injury. MATERIALS AND METHODS: An experimental controlled, randomized design was used. All studies were performed in the liquid ventilation laboratories at the University of Michigan Medical Center. Twenty-five Sprague-Dawley male rats 500+/-50 g were divided into five experimental groups: (1) CVF only (n=5), animals were cobra venom factor (CVF) lung injured; (2) PLV-CVF (n=5) animals received perflubron and PLV before CVF lung injury; (3) CVF-PLV (n=5) animals received PLV after CVF lung injury; (4) PLV only (n=5) animals underwent partial liquid ventilation without lung injury; and (5) Gas only (n=5) animals underwent gas ventilation without lung injury. In all groups iodinated bovine serum albumin (125I-BSA) was delivered by intravenous injection along with CVF or a saline placebo. RESULTS: When the CVF animals were compared with all other groups, a decrease in albumin leak was observed for all groups when compared with the CVF only controls (P < .001 by ANOVA; CVF only=1.22+/-0.12 versus PLV-CVF=0.46+/-0.08, P < .001; CVF-PLV=0.70+/-0.25, P < .001; PLV only=0.22+/-0.01, P < .001; Gas only=0.17+/-0.02, P < .001). CONCLUSIONS: These data suggest that intratracheal instillation of perfluorocarbon before or after induction of lung injury results in a reduction in pulmonary albumin leak.
Subject(s)
Capillary Leak Syndrome/physiopathology , Capillary Leak Syndrome/therapy , Fluorocarbons/therapeutic use , Respiration, Artificial/methods , Respiratory Distress Syndrome/complications , Serum Albumin, Bovine/pharmacokinetics , Analysis of Variance , Animals , Capillary Leak Syndrome/etiology , Cattle , Disease Models, Animal , Elapid Venoms , Hydrocarbons, Brominated , Instillation, Drug , Male , Random Allocation , Rats , Rats, Sprague-Dawley , Respiratory Distress Syndrome/chemically induced , TracheaABSTRACT
UNLABELLED: Acute lung injury is a frequent clinical occurrence following blood loss and trauma. The nature of this injury remains poorly understood. OBJECTIVE: To examine the relative parenchymal and intra-alveolar distribution of inflammation in a rat model of hemorrhage and resuscitation. METHODS: Rats were anesthetized and subjected to hemorrhage followed by resuscitation with shed blood and saline. Myeloperoxidase activity of lung homogenates and cytology of bronchoalveolar lavage fluid were used to measure total lung and intra-alveolar neutrophil invasion. Extravasation of i.v.-administered [125I]-albumin was used to determine total lung and alveolar permeability. Permeability results were analyzed using their base-10 logarithmic transformations. RESULTS: 86 animals were studied. Whole-lung myeloperoxidase activity was increased (control = 0.34 +/- 0.16 units, injured = 0.84 +/- 0.43 units, p < 0.01), while there was no difference in intra-alveolar leukocyte counts (injured = 1.85 +/- 1.30 x 10(5)/mL, control = 2.44 +/- 1.75 x 10(5)/mL, p = 0.40), suggesting that the cellular component of the injury was more severe in the intravascular and interstitial spaces. There was a strong trend toward increased permeability in the interstitial compartment, and a significant increase in permeability in the intra-alveolar compartment (whole-lung permeability: control = -0.27 +/- 0.19 units, injured = 0.10 +/- 0.55 units, p = 0.06; alveolar permeability: control = -2.00 +/- 0.47 units, injured = -1.32 +/- 0.49 units, p < 0.01), suggesting that the loss of integrity to macromolecules was not limited to the interstitium. CONCLUSION: Hemorrhage and resuscitation resulted in an acute lung injury characterized by extravasation of intravascular protein into both the interstitium and the intra-alveolar space. Neutrophil invasion of the lung was demonstrable only in the interstitial compartment.
Subject(s)
Lung Diseases/etiology , Lung Diseases/physiopathology , Resuscitation , Shock, Hemorrhagic/physiopathology , Animals , Blood-Air Barrier , Bronchoalveolar Lavage Fluid/immunology , Inflammation , Leukocyte Count , Lung Diseases/pathology , Male , Neutrophils , Prospective Studies , Rats , Rats, Sprague-Dawley , Shock, Hemorrhagic/etiology , Shock, Hemorrhagic/therapyABSTRACT
Pemphigus foliaceus (PF) is a dermatosis characterized by subcorneal vesicles and pathogenic IgG autoantibodies against desmoglein 1. PF IgG passively transferred into neonatal mice induces a blistering disease that duplicates the key findings of PF. In this study we have used this animal model to investigate the role of complement and IgG valence in triggering blister formation. In the passive transfer experiments, we found that PF IgG, as well as the F(ab')2 and Fab fragments, was capable of inducing the typical subcorneal blistering disease in both complement-deficient and complement-sufficient mice. Moreover, the disease activity in these mice correlated well with the dose of IgG or its proteolytic fragments injected in the animals. We conclude that neither complement activation nor IgG-mediated cell surface antigen crosslinking is required for the induction of acantholysis in the experimental PF model.
Subject(s)
Acantholysis/etiology , Acantholysis/immunology , Pemphigus/complications , Pemphigus/immunology , Acantholysis/pathology , Animals , Animals, Newborn , Autoantibodies/administration & dosage , Complement Activation , Complement C5/deficiency , Complement C5/genetics , Complement System Proteins/deficiency , Humans , Immunization, Passive , Immunoglobulin Fab Fragments/administration & dosage , Immunoglobulin G/administration & dosage , Mice , Mice, Inbred BALB C , Mice, Mutant Strains , Pemphigus/pathologyABSTRACT
Bullous pemphigoid (BP) is an inflammatory subepidermal blistering disease associated with an IgG autoimmune response to the hemidesmosomal protein, BP180. Using a passive transfer mouse model, our group has shown previously that antibodies to the murine BP180 (mBP180) ectodomain are capable of triggering a blistering skin disease that closely mimics human BP. In this study, we investigated the role of neutrophils in the immunopathogenesis of this disease model. BALB/c mice depleted of circulating neutrophils by treatment with neutrophil-specific antibodies were no longer susceptible to the pathogenic effects of anti-mBP180 IgG. IgG and complement were deposited at the dermal-epidermal junction of these animals, but there was no evidence of inflammatory infiltration or blistering. C5-deficient mice, which are resistant to the pathogenic activity of anti-mBP180 IgG, could be made susceptible to this IgG-mediated blistering disease by intradermal administration of a neutrophil chemoattractant, IL-8 or C5a. Intraperitoneal injection of IL-8, which sequesters neutrophils in the peritoneal cavity, interferes with anti-mBP180-induced neutrophilic infiltration of the skin and prevented the development of BP disease in BALB/c mice. These findings provide the first direct evidence that neutrophils recruited to the skin via a C5-dependent pathway play an essential role in subepidermal blister formation in experimental BP, and suggest new directions for disease intervention.
Subject(s)
Neutrophils/physiology , Pemphigoid, Bullous/immunology , Animals , Complement Activation , Complement C5a/physiology , Humans , Immunoglobulin G/analysis , Interleukin-8/pharmacology , Mice , Mice, Inbred BALB C , Peroxidase/metabolism , RabbitsABSTRACT
Previous studies in rats have shown that deep second degree dermal burns, involving 28-30% of total body surface area, result in systemic complement activation, appearance in plasma of chemotactic activity, sequestration of blood neutrophils in lung capillaries, and development of neutrophil-dependent dermal vascular and lung vascular injury. Although blockade of complement activation or depletion of complement before skin burns has resulted in significant attenuation of tissue injury both locally and distally (in lung), a role for C5a in these events is unclear. In the following studies, we demonstrate the presence of C5a and neutrophil chemotactic activity in serum and in lung homogenates after thermal injury. C5a has also been found in bronchoalveolar lavage fluids of thermally injured animals. Treatment of animals with a polyclonal neutralizing rabbit antibody to rat C5a was lung protective. The protective effects of the antibody (anti-C5a) were associated with diminished vascular permeability changes, as well as reduced tissue build-up of myeloperoxidase. Anti-C5a also prevented up-regulation of lung vascular ICAM-1 (intercellular adhesion molecule-1) in skin-burned rats. These observations indicate that C5a is essential for development of neutrophil accumulation and vascular permeability increases in distant (lung) organs after thermal trauma to skin. The protective effects of anti-C5a in lung, appear to be related to prevention of up-regulation of vascular ICAM-1. Accordingly, C5a may represent a target for clinical approaches in the treatment of organ injury following thermal trauma.
Subject(s)
Burns/physiopathology , Complement C5a/physiology , Neutrophils/pathology , Pulmonary Artery/pathology , Skin/pathology , Animals , Burns/blood , Capillary Permeability , Cell Movement , Rabbits , RatsABSTRACT
Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.
Subject(s)
Chemotaxis, Leukocyte , Complement Activation , Complement C5a/metabolism , Neutrophils/immunology , Animals , Antibodies/pharmacology , Chemotaxis, Leukocyte/drug effects , Complement Activation/drug effects , Complement C5a/antagonists & inhibitors , Elapid Venoms/toxicity , In Vitro Techniques , Kinetics , Lung/drug effects , Lung/immunology , Lung Injury , Macrophage-1 Antigen/metabolism , Male , Neutrophils/drug effects , Rabbits , Rats , Up-RegulationABSTRACT
Acute lung injury in rats following systemic activation of complement by i.v. infusion of cobra venom factor (CVF) is known to be P-selectin dependent. In the current studies, infusion of CVF caused the appearance in plasma of C5a (as revealed by ELISA analysis) together with neutrophil chemotactic activity, which was totally blocked by addition in vitro of anti-rat C5a. Using a detector 125I-labeled antibody to rat P-selectin, we have demonstrated quantitative up-regulation in vivo of lung vascular P-selectin in a time-dependent manner after infusion of CVF. This up-regulation was almost completely blocked by prior complement depletion or by the infusion of anti-rat C5a. Platelet depletion did not affect CVF-induced up-regulation of lung vascular P-selectin, indicating that platelets were not the source of P-selectin. These results demonstrate that complement and, specifically, C5a are necessary for up-regulation of lung vascular P-selectin after systemic activation of complement.
Subject(s)
Complement C5a/physiology , Lung/immunology , P-Selectin/blood , P-Selectin/metabolism , Up-Regulation/immunology , Animals , Cell Separation , Chemotaxis, Leukocyte/drug effects , Complement System Proteins/physiology , Humans , Lung/blood supply , Male , Mice , Mice, Inbred Strains , Neutrophils/immunology , P-Selectin/drug effects , Platelet Count , Rats , Rats, Inbred Strains , Up-Regulation/drug effectsABSTRACT
PURPOSE: To develop an animal model of complement-induced retinal vasculopathy and determine whether it resembles Purtscher's retinopathy. METHODS: Intravenous cobra venom factor was used to achieve intravascular activation of the complement system in cats. After a single bolus of cobra venom factor (75 units/kg), retinal blood flow was monitored at regular intervals by fluorescein angioscopy and angiography. RESULTS: Multiple small retinal arteriolar occlusions were present during the initial fluorescein transit of the immediate postinjection fluorescein study in 12 of 12 animals. Small, rapidly moving gaps in the fluorescein column were seen in two thirds of the animals observed continuously by fluorescein angioscopy. Angiographically, the obstructions were transient, and filling of the associated patches of capillary nonperfusion occurred within 3 minutes. Purtscher's-like ischemic retinal infarcts did not develop in any eye. Histopathologic analysis failed to demonstrate the nature of the transient vascular obstructive lesions, but indirect evidence suggested the possibility of granulocyte aggregates. CONCLUSION: Intravascular activation of the complement system produces transient microembolic retinal arteriolar occlusions in the cat. Although this model may represent a mild form of Purtscher's retinopathy, factors in addition to complement activation appear necessary to induce ischemic retinal infarcts.
Subject(s)
Complement Activation , Complement C5a/physiology , Disease Models, Animal , Granulocytes/physiology , Retinal Artery Occlusion/pathology , Angioscopy , Animals , Arterioles/metabolism , Arterioles/pathology , Blood Flow Velocity , Cats , Cell Aggregation , Complement Inactivator Proteins/toxicity , Elapid Venoms/toxicity , Embolism/complications , Embolism/pathology , Embolism/physiopathology , Female , Fluorescein , Fluorescein Angiography , Fluoresceins , Fundus Oculi , Leukocyte Count , Necrosis , Random Allocation , Retinal Artery Occlusion/etiology , Retinal Artery Occlusion/physiopathology , Retinal Vessels/metabolism , Retinal Vessels/pathologyABSTRACT
Preliminary animal experience with partial liquid ventilation (PLV) suggests that this therapy may diminish neutrophil invasion and capillary leak during acute lung injury. We sought to confirm these findings in a model of shock-induced lung injury. Sixty anesthetized rats were studied. After hemorrhage to an arterial pressure of 25 mmHg for 45 min, animals were resuscitated with blood and saline and treated with gas ventilation alone or with 5 ml/kg of intratracheally administered perflubron. Myeloperoxidase activity was used to measure lung neutrophil content. A permeability index (the bronchoalveolar-to-blood ratio of 125I-labeled albumin activity) quantified alveolar leak. Injury caused an increase in myeloperoxidase that was reversed by PLV (injury = 0.837 +/- 0.452, PLV = 0.257 +/- 0.165; P < 0.01). Capillary permeability also increased with hemorrhage, with a strong trend toward improvement in the PLV group (permeability indexes: injury = 0.094 +/- 0.102, PLV = 0.045 +/- 0.045; 95% confidence interval for injury--PLV: -0.024, 0.1219). We conclude that PLV is associated with a decrease in pulmonary neutrophil accumulation and a trend toward decreased capillary leak after hemorrhagic shock.
Subject(s)
Lung/pathology , Respiration, Artificial , Resuscitation , Shock, Hemorrhagic/pathology , Shock, Hemorrhagic/therapy , Airway Resistance/physiology , Animals , Blood Gas Analysis , Capillaries/pathology , Lung/enzymology , Male , Neutrophils/enzymology , Peroxidase/metabolism , Pulmonary Alveoli/physiology , Rats , Rats, Sprague-Dawley , Reperfusion Injury/physiopathology , Reperfusion Injury/prevention & control , Respiratory Function Tests , Serum Albumin, Radio-Iodinated , Shock, Hemorrhagic/enzymologyABSTRACT
The complement activation product, C5a, may play a key role in the acute inflammatory response. Polyclonal antibody to rat C5a was used to define the requirements for C5a in neutrophil-dependent inflammatory lung injury after systemic activation of complement by cobra venom factor (CVF) or after intrapulmonary deposition of IgG immune complexes. In the CVF model, intravenous infusion (but not intratracheal instillation) of anti-C5a produced a dose-dependent reduction in lung permeability and in lung content of myeloperoxidase. In C6-deficient rats, CVF infusion caused the same level of lung injury (measured by leak of 125I-albumin) as found in C6-sufficient rats. In the IgG immune complex model of lung injury, anti-C5a administered intratracheally (but not intravenously) reduced in a dose-dependent manner both the increase in lung vascular permeability as well as the buildup of lung myeloperoxidase. Treatment with anti-C5a greatly suppressed upregulation of lung vascular intercellular adhesion molecule-1 (ICAM-1). This was correlated with a substantial drop in levels of TNFalpha in bronchoalveolar fluids. These data demonstrate the requirement for C5a in the two models of injury. In the IgG immune complex model, C5a is required for the full production of TNFalpha and the corresponding upregulation of lung vascular ICAM-1.
Subject(s)
Complement C5a/physiology , Immunoglobulin G/pharmacology , Inflammation/physiopathology , Lung/physiopathology , Neutrophils/physiology , Animals , Bronchoalveolar Lavage Fluid/chemistry , Chemotaxis, Leukocyte , Complement Activation , Complement C5a/immunology , Elapid Venoms/administration & dosage , Elapid Venoms/toxicity , Enzyme-Linked Immunosorbent Assay , Humans , Immunoglobulin G/administration & dosage , Inflammation/immunology , Infusions, Intravenous , Instillation, Drug , Intercellular Adhesion Molecule-1/biosynthesis , Lung/drug effects , Lung/pathology , Peroxidase/metabolism , Rabbits , Rats , Trachea , Tumor Necrosis Factor-alpha/analysis , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
Bullous pemphigoid (BP) is a blistering skin disease associated with an IgG autoimmune response directed against the ectodomain of the hemidesmosomal protein, BP180. An animal model of BP has recently been developed by our laboratory based on the passive transfer of rabbit antimurine BP180 antibodies into neonatal BALB/c mice. The experimental animals develop a blistering disease that reproduces all of the key immunopathological features of BP. In the present study we have investigated the role of complement in the pathogenesis of subepidermal blistering in the mouse model of BP. We demonstrate the following. (a) Rabbit anti-murine-BP180 IgG was effective in inducing cutaneous blisters in a C5-sufficient mouse strain, but failed to induce disease in the syngeneic C5-deficient strain; (b) neonatal BALB/c mice, pretreated with cobra venom factor to deplete complement, became resistant to the pathogenic effects of the anti-BP180 IgG; (c) F(ab')2 fragments generated from the anti-BP180 IgG exhibited no pathogenic activity in the mouse model; and (d) histologic evaluation of the skin of mice described in points b and c above showed minimal or no neutrophilic cell infiltration in the upper dermis. Thus, anti-BP180 antibodies trigger subepidermal blistering in this BP model via complement activation. This experimental model of BP should greatly facilitate future studies on the pathophysiology of autoantibody-mediated diseases of the dermal-epidermal junction.