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1.
Parasites Hosts Dis ; 62(1): 131-138, 2024 Feb.
Article in English | MEDLINE | ID: mdl-38443776

ABSTRACT

Halicephalobus gingivalis is a free-living nematode that occasionally causes infections in horses. We report a rare case of limb fracture of horse caused by infection with H. gingivalis. An 8-year-old mare was referred to the Veterinary Hospital of the Federal University of Lavras with claudication grade 5 of the right hind limb, that had been started 3 months ago. The patient had aseptic arthritis in the tarsal joint and edema that extended to the quartile. The radiographic examination showed punctate osteolysis with exacerbation of bone trabeculation along the calcaneus, talus, proximal epiphysis of the third metatarsal and distal epiphysis of the tibia. Treatment for arthritis was initiated, and the animal showed a slight improvement in limb function. However, 21 days after hospitalization, due to a comminuted fracture of the tibia, it was euthanized. At necropsy, yellowish masses were found from the metatarsal to the tibia, and around the tarsal bones and joint. Similar masses were also found in the left kidney. Numerous nematodes compatible with H. gingivalis were identified. This is the first description of a pathological fracture caused by H. gingivalis infection in an equine limb.


Subject(s)
Arthritis , Fractures, Bone , Fractures, Spontaneous , Rhabditida , Animals , Female , Horses , Lower Extremity
2.
Acta Vet. Brasilica ; 15(3): 261-266, 2021. tab, graf
Article in English | VETINDEX | ID: biblio-1453295

ABSTRACT

Few information is available about the optimal period for colostrum absorption in the bowel of newborn lambs. The aim of the current study was to investigate the effects of using supplementation with commercial core homeopathic during pregnancy and early lactation on 18 pregnant Santa Inês ewes presenting body condition score ≥ 2.5. Pregnant animals were randomly divided in two groups; one was used as control (control group) and the other was supplemented on a daily basis with 30 g of homeopathic product with Natrum muriaticum 10-60, Calcium carbonicum 10-30, Silicea terra 10-400 and Hypothalamus 10-30 per animal, mixed to the concentrate during pregnancy and early lactation (treatment group). The Total Protein (TP) and globulin (GLB) values found in ewes and lambs belonging to the treatment group were higher than and different from those recorded for the control group. The serum albumin (ALB) concentration did not differ between groups of ewes but was different in lambs. TP and GLB evolution in lambs and ewes throughout the seven experimental days showed the animals in the treatment group trend to present higher TP and GLB values in all experimental periods; supplemented animals showed trend to progressive increase in both variables until day 3; whereas non-supplemented animals showed such trend until day 2 (when they reached their peak). The commercial core homeopathic to the animals during pregnancy and early lactation resulted in higher TP and GLB concentrations in the serum samples collected from ewes and lambs.


Poucas informações estão disponíveis sobre o período ideal para a absorção do colostro no intestino de neonatos ovinos. O objetivo do presente estudo é investigar os efeitos da suplementação com complexo homeopático comercial durante a gestação e início da lactação em 18 ovelhas Santa Inês gestantes com escore de condição corporal ≥ 2,5. Os animais gestantes foram divididos aleatoriamente em dois grupos; um controle (grupo controle) e outro suplementado diariamente com 30 g de produto homeopático com Natrum muriaticum 10-60, Calcium carbonicum 10-30, Silicea terra 10-400 and Hypothalamus 10-30 por animal, misturado ao concentrado durante a gestação e início da lactação (grupo tratamento). Os valores de Proteína Total (PT) e globulina (GLB) encontrados em ovelhas e cordeiros pertencentes ao grupo tratamento foram superiores e dife-rentes dos registrados para o grupo controle. A concentração de albumina sérica (ALB) não diferiu entre os grupos de ovelhas, mas foi diferente nos cordeiros. A evolução de PT e GLB em cordeiros e ovelhas ao longo dos 7 dias experimentais demons-trou que os animais do grupo tratamento tendem a apresentar maiores valores de PT e GLB em todo o período experimen-tal; os animais suplementados apresentaram tendência de aumento progressivo em ambas as variáveis até o dia 3; enquanto os animais não suplementados mostraram tal tendência até o dia 2 (quando atingiram o pico). O núcleo homeopático comercial fornecido para os animais durante a gestação e início da lactação resultou em maiores concentrações de PT e GLB nas amos-tras de soro coletadas de ovelhas e cordeiros.


Subject(s)
Female , Animals , Pregnancy , Immunologic Factors , Homeopathic Remedy , Sheep/immunology , Sheep/blood , Pregnancy, Animal/immunology , Pregnancy, Animal/blood , Immunoglobulins
3.
Acta Vet. bras. ; 15(3): 261-266, 2021. tab, graf
Article in English | VETINDEX | ID: vti-765330

ABSTRACT

Few information is available about the optimal period for colostrum absorption in the bowel of newborn lambs. The aim of the current study was to investigate the effects of using supplementation with commercial core homeopathic during pregnancy and early lactation on 18 pregnant Santa Inês ewes presenting body condition score ≥ 2.5. Pregnant animals were randomly divided in two groups; one was used as control (control group) and the other was supplemented on a daily basis with 30 g of homeopathic product with Natrum muriaticum 10-60, Calcium carbonicum 10-30, Silicea terra 10-400 and Hypothalamus 10-30 per animal, mixed to the concentrate during pregnancy and early lactation (treatment group). The Total Protein (TP) and globulin (GLB) values found in ewes and lambs belonging to the treatment group were higher than and different from those recorded for the control group. The serum albumin (ALB) concentration did not differ between groups of ewes but was different in lambs. TP and GLB evolution in lambs and ewes throughout the seven experimental days showed the animals in the treatment group trend to present higher TP and GLB values in all experimental periods; supplemented animals showed trend to progressive increase in both variables until day 3; whereas non-supplemented animals showed such trend until day 2 (when they reached their peak). The commercial core homeopathic to the animals during pregnancy and early lactation resulted in higher TP and GLB concentrations in the serum samples collected from ewes and lambs.(AU)


Poucas informações estão disponíveis sobre o período ideal para a absorção do colostro no intestino de neonatos ovinos. O objetivo do presente estudo é investigar os efeitos da suplementação com complexo homeopático comercial durante a gestação e início da lactação em 18 ovelhas Santa Inês gestantes com escore de condição corporal ≥ 2,5. Os animais gestantes foram divididos aleatoriamente em dois grupos; um controle (grupo controle) e outro suplementado diariamente com 30 g de produto homeopático com Natrum muriaticum 10-60, Calcium carbonicum 10-30, Silicea terra 10-400 and Hypothalamus 10-30 por animal, misturado ao concentrado durante a gestação e início da lactação (grupo tratamento). Os valores de Proteína Total (PT) e globulina (GLB) encontrados em ovelhas e cordeiros pertencentes ao grupo tratamento foram superiores e dife-rentes dos registrados para o grupo controle. A concentração de albumina sérica (ALB) não diferiu entre os grupos de ovelhas, mas foi diferente nos cordeiros. A evolução de PT e GLB em cordeiros e ovelhas ao longo dos 7 dias experimentais demons-trou que os animais do grupo tratamento tendem a apresentar maiores valores de PT e GLB em todo o período experimen-tal; os animais suplementados apresentaram tendência de aumento progressivo em ambas as variáveis até o dia 3; enquanto os animais não suplementados mostraram tal tendência até o dia 2 (quando atingiram o pico). O núcleo homeopático comercial fornecido para os animais durante a gestação e início da lactação resultou em maiores concentrações de PT e GLB nas amos-tras de soro coletadas de ovelhas e cordeiros.(AU)


Subject(s)
Animals , Female , Pregnancy , Sheep/blood , Sheep/immunology , Homeopathic Remedy , Immunologic Factors , Pregnancy, Animal/blood , Pregnancy, Animal/immunology , Immunoglobulins
4.
Acta sci. vet. (Impr.) ; 48(suppl.1): Pub.478-4 jan. 2020. ilus
Article in Portuguese | VETINDEX | ID: biblio-1458305

ABSTRACT

Background: Actinobacillosis in adult horses is rare, and is often associated with infection due to Actinobacillus equulisubsp. equuli, which is a commensal organism inhabiting the oral cavity of healthy animals. It is an opportunistic pathogen,and the infection usually occurs secondary to another primary disease or due to predisposing factors such as pre-existinglesions, mainly in the oral cavity. Infection may be associated with peritonitis, bacterial endocarditis, pneumonia, enteritis,infectious periorchitis, abortion, and septicemia. This paper aims to describe the clinical, microbiological, and pathologicalaspects of actinobacillosis in the oral cavity of a horse.Case: A case of actinobacillosis has been reported in a 22-year-old gelding Quarter Horse. According to the owner, theanimal presented with progressive weight loss during the previous three months, and leakage of food from the oral cavity.Examination of the oral cavity revealed sharp enamel overgrowths, which had been corrected during a previous dentalprocedure. However, five days after the procedure, the animal developed halitosis. Ceftiofur sodium (4.4 mg/kg intramuscularly, every 24 h) was prescribed; two days after the commencement of antimicrobial therapy, the animal presented withsialorrhea, bilateral submandibular lymphadenomegaly, protrusion of the tongue, edema of the base of the tongue, cheeks,and gums, in addition to black-colored sores and scales in the oral mucosa, particularly affecting the base of the tongue andgums. Samples from ulcerative lesions found at the base of the tongue and gums were collected using sterile swabs, andculture and antibiogram were requested. Microbiological culture led to the growth of small colonies measuring less than5 mm in diameter, that were non-hemolytic, slightly whitish in color but non-opaque, smooth and bright in appearance.These were identified as gram-negative rods on microscopy, and further characterized...


Subject(s)
Animals , Actinobacillus equuli/isolation & purification , Actinobacillosis , Horses/microbiology , Tongue/microbiology , Abscess/veterinary
5.
Acta sci. vet. (Online) ; 48(suppl.1): Pub. 478, 28 jan. 2020. ilus
Article in Portuguese | VETINDEX | ID: vti-25644

ABSTRACT

Background: Actinobacillosis in adult horses is rare, and is often associated with infection due to Actinobacillus equulisubsp. equuli, which is a commensal organism inhabiting the oral cavity of healthy animals. It is an opportunistic pathogen,and the infection usually occurs secondary to another primary disease or due to predisposing factors such as pre-existinglesions, mainly in the oral cavity. Infection may be associated with peritonitis, bacterial endocarditis, pneumonia, enteritis,infectious periorchitis, abortion, and septicemia. This paper aims to describe the clinical, microbiological, and pathologicalaspects of actinobacillosis in the oral cavity of a horse.Case: A case of actinobacillosis has been reported in a 22-year-old gelding Quarter Horse. According to the owner, theanimal presented with progressive weight loss during the previous three months, and leakage of food from the oral cavity.Examination of the oral cavity revealed sharp enamel overgrowths, which had been corrected during a previous dentalprocedure. However, five days after the procedure, the animal developed halitosis. Ceftiofur sodium (4.4 mg/kg intramuscularly, every 24 h) was prescribed; two days after the commencement of antimicrobial therapy, the animal presented withsialorrhea, bilateral submandibular lymphadenomegaly, protrusion of the tongue, edema of the base of the tongue, cheeks,and gums, in addition to black-colored sores and scales in the oral mucosa, particularly affecting the base of the tongue andgums. Samples from ulcerative lesions found at the base of the tongue and gums were collected using sterile swabs, andculture and antibiogram were requested. Microbiological culture led to the growth of small colonies measuring less than5 mm in diameter, that were non-hemolytic, slightly whitish in color but non-opaque, smooth and bright in appearance.These were identified as gram-negative rods on microscopy, and further characterized...(AU)


Subject(s)
Animals , Horses/microbiology , Actinobacillus equuli/isolation & purification , Actinobacillosis , Tongue/microbiology , Abscess/veterinary
6.
Rev. cient. eletrônica med. vet ; 22: 1-21, jan. 2014.
Article in Portuguese | VETINDEX | ID: biblio-1494121

ABSTRACT

Com o crescimento populacional exige-se uma demanda maior de proteína de origem animal, assim faz-se necessário o uso de ferramentas que elevem a produtividade na pecuária. O uso de aditivos na dieta de bovinos é um dos recursos mais estudados nos últimos tempos, dentre os quais a monensina é um dos mais utilizados. O presente trabalho discorre sobre a ação do aditivo monensina sódica e sua relação com a nutrição animal. Além disso, explica como os ionóforos influenciam no processo de digestão e fermentação ruminal. O tema central deste estudo é como a monensina sódica pode influenciar na fermentação ruminal, no ganho de peso dos bovinos, na prevenção e tratamento de acidose ruminal. Descreve-se também como esse aditivo pode atuar na redução de emissão de metano produzido pelo bovino.


With population growth calls for a greater demand for animal protein, so it is necessary to use tools that enhance productivity in cattle. The use of additives in the diet of cattle is one of the most studied in recent times, of which monensin is the most used. This paper discusses that the additive monensin and its relation to animal nutrition. Also, explains how ionophores influence the process of digestion and ruminal fermentation. The central theme of this study is as sodium monensin may influence rumen fermentation, the weight gain of cattle in the prevention and treatment of rumen acidosis. Describes it self as such additives may act to reduce the emission of methane from cattle.


Subject(s)
Animals , Cattle , Food Additives/analysis , Fermentation , Sodium Ionophores , Metabolism , Rumen/metabolism , Gastric Acidity Determination/veterinary
7.
Rev. cient. eletrônica med. vet ; 22: 1-16, jan. 2014. ilus, tab, graf
Article in Portuguese | VETINDEX | ID: biblio-1494123

ABSTRACT

A utilização de ferramentas que produzem retorno econômico ao ciclo produtivo da carne brasileira é voltada para o uso em conjunto da eficiência produtiva com o melhoramento genético animal aplicados ao sistema de produção no Brasil formando uma nova visão competitiva de mercado. Objetivou-se com este trabalho mostrar o aperfeiçoamento dos cruzamentos e os métodos do sistema produtivos utilizados como o manejo nutricional, sanitário e reprodutivo, posição econômica brasileira no mercado exportador de proteína animal e a cadeia produtiva. Uma boa alternativa para o associativismo econômico com enfoque na valorização do produto cárneo brasileiro cabe ao uso adequado destas técnicas.


The use of tools that produce economic returns to the productive cycle of Brazilian beef, is focused for use in conjunction of the productive efficiency with the genetic animal breeding applied to the production system in Brazil forming a new competitive market vision. The objective this work was to show the improvement and the methods used were productive system as nutrient, sanitary and reproductive management, sociocultural vision, economic position in the Brazilian market exporter of animal protein, production chain and the supply chain. A good alternative to the economic associations focusing on the value of the Brazilian meat product fits the appropriate use of these techniques.


Subject(s)
Animals , Cattle , Cost Efficiency Analysis , Brazil , Meat Industry/methods , Genetic Enhancement , Disease Prevention , Animal Feed/analysis , Breeding
8.
Rev. cient. eletrônica med. vet ; 22: 1-20, jan. 2014.
Article in Portuguese | VETINDEX | ID: biblio-1494127

ABSTRACT

Uma nova técnica vem sendo implantada no ramo do mercado cárneo brasileiro com a função de melhorar e fornecer crescimento ao país, que transforma a carne em um produto de alta qualidade para exportação. Objetivou-se com este trabalho demonstrar os meios de melhoramento para o rebanho de corte e o ganho econômico com que a técnica propicia ao produtor. O melhoramento genético que ocorre no cruzamento de bovinos ½ sangue taurino com ½ sangue zebuínos mantém uma alta heterose e retorno econômico. É utilizado pelos pecuaristas que procuram nichos de mercado e associativismo econômico, que fortaleça e valorize seu produto.


A new technique has been implemented in the business market with the Brazilian meat diet to improve function and provide growth to the country, which makes the meat in a high quality product for export. The objective of this work demonstrate the means of improvement to the flock cutting and economic gain that the technique provides the producer. The breeding that occurs at the intersection of bovine ½ blood taurine and ½ blood Zebu heterosis and maintains a high economic return. It is used by farmers looking for niche markets and economic association that strengthens and enhances your product.


Subject(s)
Animals , Cattle , Cost Efficiency Analysis , Crosses, Genetic , Genetic Enhancement/methods , Selective Breeding , Hybrid Vigor , Food Quality , Reproductive Techniques/veterinary
9.
R. cient. eletr. Med. Vet. ; 22: 1-20, jan. 2014.
Article in Portuguese | VETINDEX | ID: vti-13368

ABSTRACT

Uma nova técnica vem sendo implantada no ramo do mercado cárneo brasileiro com a função de melhorar e fornecer crescimento ao país, que transforma a carne em um produto de alta qualidade para exportação. Objetivou-se com este trabalho demonstrar os meios de melhoramento para o rebanho de corte e o ganho econômico com que a técnica propicia ao produtor. O melhoramento genético que ocorre no cruzamento de bovinos ½ sangue taurino com ½ sangue zebuínos mantém uma alta heterose e retorno econômico. É utilizado pelos pecuaristas que procuram nichos de mercado e associativismo econômico, que fortaleça e valorize seu produto. (AU)


A new technique has been implemented in the business market with the Brazilian meat diet to improve function and provide growth to the country, which makes the meat in a high quality product for export. The objective of this work demonstrate the means of improvement to the flock cutting and economic gain that the technique provides the producer. The breeding that occurs at the intersection of bovine ½ blood taurine and ½ blood Zebu heterosis and maintains a high economic return. It is used by farmers looking for niche markets and economic association that strengthens and enhances your product. (AU)


Subject(s)
Animals , Cattle , Genetic Enhancement/methods , Crosses, Genetic , Hybrid Vigor , Cost Efficiency Analysis , Selective Breeding , Food Quality , Reproductive Techniques/veterinary
10.
R. cient. eletr. Med. Vet. ; 22: 1-16, jan. 2014. ilus, tab, graf
Article in Portuguese | VETINDEX | ID: vti-691195

ABSTRACT

A utilização de ferramentas que produzem retorno econômico ao ciclo produtivo da carne brasileira é voltada para o uso em conjunto da eficiência produtiva com o melhoramento genético animal aplicados ao sistema de produção no Brasil formando uma nova visão competitiva de mercado. Objetivou-se com este trabalho mostrar o aperfeiçoamento dos cruzamentos e os métodos do sistema produtivos utilizados como o manejo nutricional, sanitário e reprodutivo, posição econômica brasileira no mercado exportador de proteína animal e a cadeia produtiva. Uma boa alternativa para o associativismo econômico com enfoque na valorização do produto cárneo brasileiro cabe ao uso adequado destas técnicas. (AU)


The use of tools that produce economic returns to the productive cycle of Brazilian beef, is focused for use in conjunction of the productive efficiency with the genetic animal breeding applied to the production system in Brazil forming a new competitive market vision. The objective this work was to show the improvement and the methods used were productive system as nutrient, sanitary and reproductive management, sociocultural vision, economic position in the Brazilian market exporter of animal protein, production chain and the supply chain. A good alternative to the economic associations focusing on the value of the Brazilian meat product fits the appropriate use of these techniques. (AU)


Subject(s)
Animals , Cattle , Genetic Enhancement , Animal Feed/analysis , Disease Prevention , Cost Efficiency Analysis , Meat Industry/methods , Brazil , Breeding
11.
R. cient. eletr. Med. Vet. ; 22: 1-21, jan. 2014.
Article in Portuguese | VETINDEX | ID: vti-691193

ABSTRACT

Com o crescimento populacional exige-se uma demanda maior de proteína de origem animal, assim faz-se necessário o uso de ferramentas que elevem a produtividade na pecuária. O uso de aditivos na dieta de bovinos é um dos recursos mais estudados nos últimos tempos, dentre os quais a monensina é um dos mais utilizados. O presente trabalho discorre sobre a ação do aditivo monensina sódica e sua relação com a nutrição animal. Além disso, explica como os ionóforos influenciam no processo de digestão e fermentação ruminal. O tema central deste estudo é como a monensina sódica pode influenciar na fermentação ruminal, no ganho de peso dos bovinos, na prevenção e tratamento de acidose ruminal. Descreve-se também como esse aditivo pode atuar na redução de emissão de metano produzido pelo bovino. (AU)


With population growth calls for a greater demand for animal protein, so it is necessary to use tools that enhance productivity in cattle. The use of additives in the diet of cattle is one of the most studied in recent times, of which monensin is the most used. This paper discusses that the additive monensin and its relation to animal nutrition. Also, explains how ionophores influence the process of digestion and ruminal fermentation. The central theme of this study is as sodium monensin may influence rumen fermentation, the weight gain of cattle in the prevention and treatment of rumen acidosis. Describes it self as such additives may act to reduce the emission of methane from cattle. (AU)


Subject(s)
Animals , Cattle , Fermentation , Rumen/metabolism , Food Additives/analysis , Sodium Ionophores , Metabolism , Gastric Acidity Determination/veterinary
12.
Acta Vet. Brasilica ; 8(4): 231-235, 2014.
Article in Portuguese | VETINDEX | ID: biblio-1453524

ABSTRACT

We evaluated the pregnancy rate, circumference of corpora lutea and formation of accessory corpora lutea (ACL) in Nelore heifers treated with human chorionic gonadotropin (hCG) seven days after fixedtime artificial insemination (FTAI). 137 heifers were used, with an average weight of 308.07 ±12.86kg, were randomly assigned into two groups, with Group A consisting of 67 heifers receiving hCG seven days after AI and Group B with 70 heifers receiving solution saline seven days after AI. The synchronization of follicular wave and ovulation were performed in the morning (8:00 a.m.) and afternoon (14:00 p.m.), respectively for Groups A and B. On Day 10 (D10), 48 hours after the removal of the implants vaginal heifers in Groups A and B were inseminated in the morning and afternoon respectively. On day 17 (D17) in the morning in Groups A were applied intramuscularly 1500UI (1.5 mL) of hCG and in the evening 1.5 mL saline in Group B. After 11 days (D28) by transrectal ultrasonography, we evaluated the existence, number and circumference of corpus luteum present in the ovaries of heifers in Groups A and B. Pregnancy diagnosis was performed 35 days after AI (D45). The application of hCG in heifers seven days after FTAI induced the formation of CLA had no effect on the size of corpus luteum observed in pregnant females treated or not and did not improve the pregnancy rate of those females.


Avaliou-se a taxa de gestação, circunferência dos corpos lúteos e formação de corpos lúteos acessório (CLA) em novilhas da raça Nelore, tratadas com Gonadotrofina Coriônica Humana (hCG) sete dias após inseminação artificial em tempo fixo (IATF). Foram utilizadas 137 novilhas, com peso médio de 308,07±12,86 kg, distribuídas aleatoriamente em dois Grupos, sendo o Grupo A composto por 67 novilhas que receberam hCG sete dias após a IATF e o Grupo B de 70 novilhas que receberam solução salina sete dias após a IATF. As sincronizações da onda folicular e ovulação foram realizadas pela manhã (8h00) e tarde (14h00) respectivamente para os Grupos A e B. No Dia 10 (D10), 48 horas após a retirada dos implantes vaginais, as novilhas dos Grupos A e B foram inseminadas pela manhã e tarde, respectivamente. No Dia 17 (D17), pela manhã nos animais do Grupo A, foram aplicados por via intramuscular 1500UI (1,5mL) de hCG e à tarde 1,5mL de solução salina nos animais do Grupo B. Após 11 dias (D28), por ultra-sonografia transretal, foi avaliada a existência, o número e a circunferência dos corpos lúteos presentes nos ovários das novilhas dos Grupos A e B. O diagnóstico de gestação foi realizado 35 dias após a IATF (D45). A aplicação de hCG em novilhas sete dias após a IATF induziu a formação de CLA, não interferiu no tamanho dos corpos lúteos observados nas fêmeas gestantes tratadas e não tratadas e não melhorou a taxa de gestação das referidas fêmeas.


Subject(s)
Female , Animals , Cattle , Corpus Luteum/anatomy & histology , Corpus Luteum/growth & development , Chorionic Gonadotropin/therapeutic use , Pregnancy Rate
13.
Acta Vet. bras. ; 8(4): 231-235, 2014.
Article in Portuguese | VETINDEX | ID: vti-379123

ABSTRACT

We evaluated the pregnancy rate, circumference of corpora lutea and formation of accessory corpora lutea (ACL) in Nelore heifers treated with human chorionic gonadotropin (hCG) seven days after fixedtime artificial insemination (FTAI). 137 heifers were used, with an average weight of 308.07 ±12.86kg, were randomly assigned into two groups, with Group A consisting of 67 heifers receiving hCG seven days after AI and Group B with 70 heifers receiving solution saline seven days after AI. The synchronization of follicular wave and ovulation were performed in the morning (8:00 a.m.) and afternoon (14:00 p.m.), respectively for Groups A and B. On Day 10 (D10), 48 hours after the removal of the implants vaginal heifers in Groups A and B were inseminated in the morning and afternoon respectively. On day 17 (D17) in the morning in Groups A were applied intramuscularly 1500UI (1.5 mL) of hCG and in the evening 1.5 mL saline in Group B. After 11 days (D28) by transrectal ultrasonography, we evaluated the existence, number and circumference of corpus luteum present in the ovaries of heifers in Groups A and B. Pregnancy diagnosis was performed 35 days after AI (D45). The application of hCG in heifers seven days after FTAI induced the formation of CLA had no effect on the size of corpus luteum observed in pregnant females treated or not and did not improve the pregnancy rate of those females.(AU)


Avaliou-se a taxa de gestação, circunferência dos corpos lúteos e formação de corpos lúteos acessório (CLA) em novilhas da raça Nelore, tratadas com Gonadotrofina Coriônica Humana (hCG) sete dias após inseminação artificial em tempo fixo (IATF). Foram utilizadas 137 novilhas, com peso médio de 308,07±12,86 kg, distribuídas aleatoriamente em dois Grupos, sendo o Grupo A composto por 67 novilhas que receberam hCG sete dias após a IATF e o Grupo B de 70 novilhas que receberam solução salina sete dias após a IATF. As sincronizações da onda folicular e ovulação foram realizadas pela manhã (8h00) e tarde (14h00) respectivamente para os Grupos A e B. No Dia 10 (D10), 48 horas após a retirada dos implantes vaginais, as novilhas dos Grupos A e B foram inseminadas pela manhã e tarde, respectivamente. No Dia 17 (D17), pela manhã nos animais do Grupo A, foram aplicados por via intramuscular 1500UI (1,5mL) de hCG e à tarde 1,5mL de solução salina nos animais do Grupo B. Após 11 dias (D28), por ultra-sonografia transretal, foi avaliada a existência, o número e a circunferência dos corpos lúteos presentes nos ovários das novilhas dos Grupos A e B. O diagnóstico de gestação foi realizado 35 dias após a IATF (D45). A aplicação de hCG em novilhas sete dias após a IATF induziu a formação de CLA, não interferiu no tamanho dos corpos lúteos observados nas fêmeas gestantes tratadas e não tratadas e não melhorou a taxa de gestação das referidas fêmeas.(AU)


Subject(s)
Animals , Female , Cattle , Pregnancy Rate , Corpus Luteum/anatomy & histology , Corpus Luteum/growth & development , Chorionic Gonadotropin/therapeutic use
14.
Vet Microbiol ; 118(1-2): 151-7, 2006 Nov 26.
Article in English | MEDLINE | ID: mdl-16919898

ABSTRACT

Shiga toxin producing-Escherichia coli (STEC) is an important emerging pathogen, and ruminants are recognized as their main natural reservoir. The aim of this work was to establish the frequency of STEC in non-domestic mammals of the Zoo and Botanical Garden of La Plata City, Argentina, and to pheno-genotypically characterize STEC isolates. By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 50.8% of 65 fecal samples. Twenty-five STEC strains were isolated from 38.5% of the Zoo's animals. Ten species of order Cetartiodactyla and one species of order Rodentia were recognized as new STEC carriers. STEC strains belonged to 7 different serotypes including new serotypes O12:H25 and O13:H6. Serotype O146:H28, previously associated with human infections, represented 24% of STEC isolates. The most frequent Shiga toxin identified were type 1c and type 2c. Nineteen strains were positive for iha gene, 8 strains were positive for ehxA gene. Moreover, all strains were positive for lpfAO113 and negative for rfbO157, eae, saa, lpfAO157/OI-141, lpfAO157/OI-154, efa1, and toxB genes. Results obtained by XbaI-pulsed-field gel electrophoresis (XbaI-PFGE) confirmed the transmission of STEC strains among different animal species and suborders. In addition, we observed a potential association between STEC-harboring animal and factors such as belonging to order Cetartiodactyla, living in a pit, and belonging to a non-autochthonous species. This is the first work developed with zoological mammals and STEC in Argentina.


Subject(s)
Artiodactyla/microbiology , Escherichia coli Infections/veterinary , Escherichia coli/isolation & purification , Rodent Diseases/microbiology , Shiga Toxins/biosynthesis , Animals , Animals, Zoo/microbiology , Argentina , Disease Reservoirs/veterinary , Electrophoresis, Gel, Pulsed-Field/veterinary , Escherichia coli Infections/diagnosis , Escherichia coli Infections/microbiology , Escherichia coli Infections/transmission , Feces/microbiology , Rodent Diseases/diagnosis , Rodent Diseases/transmission , Rodentia , Serotyping/veterinary , Species Specificity
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