ABSTRACT
The aryl hydrocarbon receptor (AhR) is commonly described as a transcription factor, which regulates xenobiotic-metabolizing enzymes. Recent studies have suggested that the binding of ligands to the AhR also activates the Src kinase. In this manuscript, we show that the AhR, through the activation of Src, activates focal adhesion kinase (FAK) and promotes integrin clustering. These effects contribute to cell migration. Further, we show that the activation of the AhR increases the interaction of FAK with the metastatic marker, HEF1/NEDD9/CAS-L, and the expression of several integrins. Xenobiotic exposure, thus, may contribute to novel cell-migratory programs.
Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Focal Adhesion Protein-Tyrosine Kinases/metabolism , Focal Adhesions/physiology , Integrin beta1/metabolism , Phosphoproteins/metabolism , Receptors, Aryl Hydrocarbon/metabolism , src-Family Kinases/metabolism , Adaptor Proteins, Signal Transducing/genetics , Apoptosis , Blotting, Western , Cell Adhesion , Cell Movement , Cell Proliferation , Fluorescent Antibody Technique , Focal Adhesion Protein-Tyrosine Kinases/genetics , Hep G2 Cells , Humans , Immunoenzyme Techniques , Immunoprecipitation , Integrin beta1/genetics , Phosphoproteins/genetics , Phosphorylation , RNA, Messenger/genetics , Real-Time Polymerase Chain Reaction , Receptors, Aryl Hydrocarbon/genetics , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction , src-Family Kinases/geneticsABSTRACT
Aryl hydrocarbon receptor (AhR), or dioxin receptor, is a transcription factor that induces adaptive metabolic pathways in response to environmental pollutants. Recently, other pathways were found to be altered by AhR and its ligands. Indeed, developmental defects elicited by AhR ligands suggest that additional cellular functions may be targeted by this receptor, including cell migration and plasticity. Here, we show that dioxin-mediated activation of Ahr induces Nedd9/Hef1/Cas-L, a member of the Cas protein family recently identified as a metastasis marker. The Hef1 gene induction is mediated by two xenobiotic responsive elements present in this gene promoter. Moreover, using RNA interference, we show that Nedd9/Hef1/Cas-L mediates the dioxin-elicited changes related to cell plasticity, including alterations of cellular adhesion and shape, cytoskeleton reorganization, and increased cell migration. Furthermore, we show that both E-cadherin repression and Jun N-terminal kinases activation by dioxin and AhR also depend on the expression of Nedd9/Hef1/Cas-L. Our study unveils, for the first time, a link between pollutants exposure and the induced expression of a metastasis marker and shows that cellular migration and plasticity markers are regulated by AhR and its toxic ligands.
Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Cell Movement/drug effects , Environmental Pollutants/toxicity , Phosphoproteins/metabolism , Adaptor Proteins, Signal Transducing/deficiency , Adaptor Proteins, Signal Transducing/genetics , Animals , Cell Line, Tumor , Dioxins/toxicity , Down-Regulation , Gene Knockdown Techniques , Humans , Phosphoproteins/deficiency , Phosphoproteins/genetics , RNA Interference , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Aryl Hydrocarbon/deficiency , Receptors, Aryl Hydrocarbon/genetics , Receptors, Aryl Hydrocarbon/metabolism , Transcriptional Activation/drug effectsABSTRACT
The objective of the study was to evaluate the effect of mycophenolate mofetil (MMF) on the regulation of inosine monophosphate dehydrogenase (IMPDH) during the first 2 years after renal transplantation. Twelve patients were enrolled, and 10-h time-course evaluations of the effects of MMF were regularly performed during the study. IMPDH activity and gene expression were measured in whole blood and in mononuclear cells, respectively. Type I IMPDH (IMPDH-I) mRNA was increased during the first 3 months following transplantation and reached its maximal level during acute rejection episodes, whereas type II IMPDH mRNA was stable. Furthermore, although no alteration in the predose samples was observed, patients with prolonged MMF treatment exhibited an increase in the induction potency of both IMPDH activity and gene expression. In vitro experiments confirmed that IMPDH-I is inducible, but preferentially in monocytes than in lymphocytes. This finding suggests that the measurement of IMPDH mRNAs may provide reliable information to predict acute rejection.
Subject(s)
Gene Expression Regulation, Enzymologic/drug effects , Graft Rejection/prevention & control , IMP Dehydrogenase/blood , Immunosuppressive Agents/therapeutic use , Kidney Transplantation , Leukocytes, Mononuclear/drug effects , Mycophenolic Acid/analogs & derivatives , Adult , Aged , Biomarkers/blood , Cells, Cultured , Female , Follow-Up Studies , Graft Rejection/enzymology , Humans , IMP Dehydrogenase/genetics , Immunosuppressive Agents/pharmacokinetics , Leukocytes, Mononuclear/enzymology , Male , Middle Aged , Mycophenolic Acid/pharmacokinetics , Mycophenolic Acid/therapeutic use , Prospective Studies , RNA, Messenger/blood , Time Factors , Treatment Outcome , Up-RegulationABSTRACT
In several regions of the developing nervous system, neurons undergo programmed cell death. In the rat cerebellum, Purkinje cell apoptosis is exacerbated when cerebellar slices are cultured during the first postnatal week. To understand the mechanism of this developmental apoptosis, we took advantage of its inhibition by the steroid analog mifepristone. This effect did not involve the classical steroid nuclear receptors. Microarray analysis revealed that mifepristone down-regulated mRNA levels of the Na+/K+-ATPase alpha3 subunit more than three times. Consistent with the down-regulation of the Na+/K+-ATPase, mifepristone caused Purkinje cell membrane depolarization. Depolarizing agents like ouabain (1 microM), tetraethylammonium (2 mM), and veratridine (2 microM) protected Purkinje cells from apoptosis. These results suggest a role of excitatory inputs in Purkinje cell survival during early postnatal development. Indeed, coculturing cerebellar slices with glutamatergic inferior olivary neuron preparations allowed rescue of Purkinje cells. These findings reveal a new neuroprotective mechanism of mifepristone and support a pivotal role for excitatory inputs in the survival of Purkinje neurons. Mifepristone may be a useful lead compound in the development of novel therapeutic approaches for maintaining the resting potential of neurons at values favorable for their survival under neuropathological conditions.
Subject(s)
Membrane Potentials/physiology , Mifepristone/pharmacology , Neurons/physiology , Purkinje Cells/physiology , Animals , Animals, Newborn , Cell Survival/drug effects , Cells, Cultured , Cerebellum/physiology , Gene Expression Regulation, Enzymologic/drug effects , Hormone Antagonists/pharmacology , In Vitro Techniques , Membrane Potentials/drug effects , Neurons/drug effects , Olivary Nucleus/drug effects , Olivary Nucleus/physiology , Purkinje Cells/drug effects , Rats , Sodium-Potassium-Exchanging ATPase/geneticsABSTRACT
Environmental chemicals such as dioxin adversely affect immune, neurological and reproductive functions and have been implicated in cancer development. However, the mechanisms responsible for dioxin toxicity are still poorly understood. Here, we show that dioxin and related pollutants trigger a marked morphological change in epithelial cells that remodel their cytoskeleton to increase interaction with extra cellular matrix while loosening cell-cell contacts. Furthermore, dioxin-treated cells show increased motility. These dioxin-mediated effects are mimicked by constitutive expression and activation of the intracellular dioxin receptor (aryl hydrocarbon receptor (AhR)). They correlate with activation of the Jun NH2-terminal kinase (JNK) and are reverted by treatment with a JNK inhibitor. Dioxin-induced effects occur 48 h post-treatment initiation, a time scale, which argues for a genomic effect of the AhR, linked to induction of target genes. This novel Ahr action on cell plasticity points to a role in cancer progression.
Subject(s)
JNK Mitogen-Activated Protein Kinases/metabolism , Methylcholanthrene/toxicity , Polychlorinated Dibenzodioxins/metabolism , Receptors, Aryl Hydrocarbon/metabolism , Actins/metabolism , Benz(a)Anthracenes/metabolism , Cadherins/metabolism , Cell Differentiation , Cell Line, Tumor , Cell Movement , Down-Regulation , Epithelial Cells/cytology , Epithelial Cells/metabolism , Humans , Ligands , Methylcholanthrene/metabolism , Polychlorinated Dibenzodioxins/toxicity , Receptors, Aryl Hydrocarbon/genetics , Receptors, Estrogen/metabolism , Signal Transduction , Smoke , NicotianaABSTRACT
Recent epidemiological studies have revealed a possible correlation between exposure to high levels of dioxins or dioxin-like compounds and diabetes. Yet the interaction between insulin and dioxin actions remains elusive. We studied the regulation of insulin-like growth factor binding protein-1 (IGFBP-1), a protein involved in glucose homeostasis and whose expression is down-regulated by insulin. We showed that 2,3,7,8-tetrachorodibenzo-p-dioxin (TCDD) specifically induced IGFBP-1 mRNA in human hepatocytes and HepG2 human hepatoma cells (2.5- and 8-fold, respectively). Cellular and secreted IGFBP-1 protein levels were also up-regulated. Transfection and reporter assays showed that the IGFBP-1 promoter was activated by TCDD and that this activation was dependent on the integrity of a proximal xenobiotic-responsive element (XRE). This XRE, located near the insulin-glucocorticoid regulatory region, binds the aryl-hydrocarbon receptor. In agreement with previous studies, the IGFBP-1 promoter was down-regulated by insulin (50%); we show here that although TCDD activated the IGFBP-1 promoter 5- to 6-fold, the combination of TCDD and insulin led to an expression level of IGFBP-1 that was higher than basal level (2- to 3-fold activation). Similar regulations were observed for the endogenous IGFBP-1 mRNA. These data suggest that the xenobiotic-hormonal regulatory region of the IGFBP-1 promoter mediates an up-regulation of IGFBP-1 expression by TCDD even in the presence of insulin. Because IGFBP-1 modulates blood glucose levels, the up-regulation of IGFBP-1 by dioxins might account for the disruptive effects of these pollutants on glucose metabolism.
Subject(s)
Insulin-Like Growth Factor Binding Proteins/antagonists & inhibitors , Insulin-Like Growth Factor Binding Proteins/biosynthesis , Insulin/pharmacology , Polychlorinated Dibenzodioxins/pharmacology , Pregnancy Proteins/antagonists & inhibitors , Pregnancy Proteins/biosynthesis , Cell Line, Tumor , Down-Regulation/drug effects , Gene Expression Regulation/drug effects , Gene Expression Regulation/physiology , Humans , Insulin-Like Growth Factor Binding Protein 1 , Insulin-Like Growth Factor Binding Proteins/genetics , Insulin-Like Growth Factor Binding Proteins/metabolism , Pregnancy Proteins/genetics , Pregnancy Proteins/metabolism , Promoter Regions, Genetic/drug effects , Protein Binding , RNA, Messenger/antagonists & inhibitors , RNA, Messenger/biosynthesis , Receptors, Aryl Hydrocarbon/metabolismABSTRACT
A novel approach to reducing organ toxicity of anticancer agents is the application of nontoxic glucuronide prodrugs from which the active drug is released by human beta-glucuronidase, an enzyme present at high levels in many tumors. In view of high interindividual variability in beta-glucuronidase expression, regulation of this enzyme is an essential factor modulating bioactivation of glucuronide prodrugs. However, data on regulation of human beta-glucuronidase expression are not available. Preliminary evidence from animal experiments points to a role of intracellular calcium in regulation of beta-glucuronidase activity. Therefore, we investigated regulation of beta-glucuronidase by the calcium ionophore A23187 and the calcium ATPase inhibitor thapsigargin in the human hepatoma cell line HepG2. The enzyme was characterized on activity, protein, and mRNA levels by cleavage of 4-methylumbelliferyl-beta-D-glucuronide, Western blotting, Northern blotting, and nuclear run-on transcription. Incubation of HepG2 cells with A23187 and thapsigargin, respectively, revealed a time and concentration dependent down-regulation of beta-glucuronidase activity to about 50% of the control level. This effect could also be demonstrated in several other cell lines (e.g., HL-60, ECV 304, 32M1, Caco-2/TC7). Effects on protein and mRNA levels paralleled those obtained on enzymatic activity. In line with these data, A23187 and thapsigargin decreased beta-glucuronidase transcriptional rate. Our data demonstrate regulation of human beta-glucuronidase by xenobiotics. Down-regulation of beta-glucuronidase by A23187 and thapsigargin is at least partly mediated by a transcriptional mechanism. Based on our findings, we speculate that beta-glucuronidase activity and hence bioactivation of glucuronide prodrugs in humans can be modulated by exogenous factors.
Subject(s)
Calcimycin/pharmacology , Gene Expression Regulation, Enzymologic/drug effects , Glucuronidase/genetics , Thapsigargin/pharmacology , Blotting, Northern , Blotting, Western , Carcinoma, Hepatocellular , Dose-Response Relationship, Drug , Down-Regulation , Enzyme Inhibitors/pharmacology , Glucuronidase/drug effects , Glucuronidase/metabolism , Humans , Ionophores/pharmacology , Lung Neoplasms , RNA, Messenger/drug effects , RNA, Messenger/metabolism , Time Factors , Transcription, Genetic/drug effects , Tumor Cells, CulturedABSTRACT
Fibrates modify the expression of genes implicated in lipoprotein and fatty acid metabolism via the peroxisome proliferator-activated receptor alpha(PPARalpha), leading to reductions in serum triglycerides and cholesterol. The expression of certain genes regulated by PPARalpha have been shown to be modified in a species dependent manner. Aspartate aminotransferase (AspAT or GOT) and alanine aminotransferase (AlaAT or GPT) are enzymes involved in intermediate metabolism in all cells and in hepatic gluconeogenesis. These enzymes are also widely used as serum markers of possible tissue damage. This study investigated whether fenofibrate could modify the expression of liver AspAT and/or AlaAT and thus possibly alter transaminase levels independently of a cytotoxic effect. In human Hep G2 cells, fenofibrate increased cytosolic AspAT (cAspAT) activity by 40% and AlaAT activity by 100%, as well as both mRNAs. Nuclear run on assays showed that this effect was, at least in part, transcriptional. Increases in mRNA were also observed in human hepatocyte cultures at concentrations of the drug attained in patients. In C57BL/6 mice, fenofibrate decreased cAspAT and cAlaAT mRNA, while these effects were abolished in PPARalpha knock-out mice. In conclusion, fenofibrate has been shown to modify cAspAT and AlaAT gene expression in a species and PPARalpha dependent manner. This is the first demonstration that cAspAT and AlaAT activities may be pharmacologically altered, independently of a toxic phenomenon.
Subject(s)
Fenofibrate/pharmacology , Gene Expression Regulation, Enzymologic/drug effects , Hypolipidemic Agents/pharmacology , Nuclear Proteins/physiology , Receptors, Cytoplasmic and Nuclear/physiology , Transaminases/biosynthesis , Transaminases/genetics , Transcription Factors/physiology , Alanine Transaminase/biosynthesis , Alanine Transaminase/genetics , Animals , Aspartate Aminotransferases/biosynthesis , Aspartate Aminotransferases/genetics , Blotting, Northern , Cells, Cultured , Humans , Liver/cytology , Liver/drug effects , Liver/metabolism , Mice , Nuclear Proteins/deficiency , Nuclear Proteins/genetics , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Receptors, Cytoplasmic and Nuclear/deficiency , Receptors, Cytoplasmic and Nuclear/genetics , Transcription Factors/deficiency , Transcription Factors/genetics , Tumor Cells, CulturedABSTRACT
Five phages which are morphologically similar to coliphage T7 but attack other host bacteria have been compared to T7 and to its relative, T3, by the following criteria: (a) cross-reactivity with antisera against T7 and T3, (b) DNA base sequence homologies, as determined by the C0t technique, (c) synthesis of two phage-coded enzymes: RNA polymerase and SAMase, (d) patterns of phage-directed protein synthesis, as determined by SDS-polyacrylamide gel electrophoresis of phage coat subunits. As judged by all these criteria, Pseudomonas phage PX3 is not related to T7; thus, morphological similarity was attributed to convergent evolution. The other phages, i.e. Serratia phage IV, Psuedomonas phage gh-1, Citrobacter phage ViIII and Klebsiella phage No. 11, were considered to be related to T7 on the basis of similarities in the patterns of phage-coded proteins and because, early after infection, these phages induced, as T7 does, an RNA polymerase which specifically transcribes the DNA of thehomologous phage. Phages IV and No. 11 also induced the early synthesis of SAMase (previously only known to occur upon T3 infection). With the exception of phage IV, however, DNA base sequence homologies with T7 or T3 seem to be poor or non-existent. The tested phages, again with the exception of phage IV, did not react with antiserum against T3 or T7. It is concluded that a particular pattern of phage-directed protein synthesis (as characterized by polyacrylamide gel electrophoresis and enzyme tests) may provide evidence for phylogenetic relationships between phages, even in cases where other criteria, such as genetic recombination, serological cross-reaction, and DNA base sequence homologies, fail to indicate relatedness.