ABSTRACT
The capacity of 36 Cuban strains of Streptococcus pneumoniae to produce pneumolysin was studied, and 94.4% of them were determined to be producers of that enzyme. One of the best producers was cultured at a great scale and the pneumolysin found in the supernatan was partially purified through an ion-exchange chromatography in mono-Q column. This method made it possible to recover the enzyme whose purity level increased by 4.39 with 100% output.
Subject(s)
Cytotoxins/isolation & purification , Streptococcus pneumoniae/metabolism , Streptolysins/isolation & purification , Bacterial Proteins , Cytotoxins/biosynthesis , Humans , Streptolysins/biosynthesisABSTRACT
The specific type of capsular polysaccharide of an autochthonous strain of Cryptococcus neoformans var. neoformans was obtained by using the method of selective precipitation with hexadimethrine bromide. The capsular polysaccharide was matched to lamb's erythrocytes and it was used as an immunogen in rabbits. Antibody titres of up to 1:32 were attained. Doble serial dilutions of the capsular polysaccharide were evaluated as positive control antigen by contraimmunoelectrophoresis, latex and ELISA techniques, showing biological activity.
Subject(s)
Antigens, Fungal/immunology , Cryptococcus neoformans/immunology , Polysaccharides/immunology , Animals , Antibodies, Fungal/biosynthesis , Antibodies, Fungal/blood , Antigens, Fungal/isolation & purification , Erythrocytes/immunology , Immunization/methods , Polysaccharides/isolation & purification , Rabbits , SheepABSTRACT
A growth supplement for "fastidious germs" was produced at the Laboratory of Acute Respiratory Infections of "Pedro Kourí" Institute of Tropical Medicine. This supplement was studied by computerized optic spectrophotometry, and chemical composition was determined. The efficacy of this supplement for the culture of Haemophilus influenzae type b, was evidenced using 100 strains, and it was proven that it can be used in concentrations raging from 1 to 10%.
