ABSTRACT
BACKGROUND: Lymphatic neoplasia may occur in various types, such as lymphoma, lymphosarcoma, lympholeukemia, and plasmacytoid leukemia. Lymphoma, defined as a malignant tumour of lymphoid tissue, has been found in a number of fish families including Esocidae and Salmonidae. However, the occurrence of lymphoma is rare in those belonging to the Cyprinidae. A final diagnosis of ocular and testicular T-cell lymphoma in the present study was based on the clinical signs, morphology, and texture of the tumour masses in the macroscopic and microscopic examinations. In addition, histopathological and immunohistochemical findings corresponded to T-cell lymphoma characteristics. CASE PRESENTATION: A 2-year-old hermaphrodite koi carp (Cyprinus carpio Linnaeus 1758) with a large ocular mass and severe exophthalmia in the right eye was referred to the Ornamental Fish Clinic in October 2020. Under anesthesia, enucleation was performed. 57 days after enucleation of the right eye, exophthalmia in the left eye was discovered. 221 days after surgery, the fish was discovered to be dead. At necropsy, a large soft tissue mass attached to the left testis was discovered. There were also small whitish nodules on the surface of the liver. Histopathology revealed a hypercellular ocular mass with scant connective tissue. The sections also revealed multifocal hemorrhages, round to ovoid neoplastic cells, mild-to-moderate anisokaryosis and anisocytosis, and mitotic figures. Basophilic neoplastic cells were found in blood vessels within the testicular mass, raising the possibility of systemic spread. The liver showed microscopic metastasis with morphologic similarities to the ocular and testicular tumors. The neoplastic cells infiltrating the left and right eyes as well as the testicular mass were immunohistochemically positive for CD3 but negative for CD20. The masses were diagnosed as T-cell lymphoma based on histopathological and immunohistochemical findings. CONCLUSIONS: This case report provides the first evidence of clinical, histopathological, morphological, and immunohistochemical findings of an ocular and testicular T-cell lymphoma in a hermaphrodite koi carp (Cyprinus carpio) in Iran.
Subject(s)
Eye Neoplasms , Fish Diseases , Lymphoma, T-Cell, Peripheral , Testicular Neoplasms , Animals , Male , Carps , Fish Diseases/pathology , Iran , Lymphoma, T-Cell, Peripheral/pathology , Lymphoma, T-Cell, Peripheral/veterinary , Testicular Neoplasms/pathology , Testicular Neoplasms/veterinary , Eye Neoplasms/pathology , Eye Neoplasms/veterinary , Immunohistochemistry/veterinaryABSTRACT
Myxoma is a benign mesenchymal tumor of skin and soft connective tissues considered to be of fibroblastic origin. A female bearded dragon (Pogona vitticeps) with a raised mass on the left side of the body was referred to the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. The mass had become evident and grew more extensive within the previous 11-month period. The dragon was anesthetized with 5.00% isoflurane, and the mass was removed surgically. Tissue samples of the mass were fixed in 10.00% neutral buffered formalin, sectioned and stained with Hematoxylin and Eosin (H&E), Alcian blue (pH: 2.50), and periodic acid Schiff (PAS) staining techniques. The patient was administered meloxicam, lincomycin, and marbofloxacin. Histopathologically, the capsulated mass was composed of myxoid structure. Stellate-to-reticular cells distributed in a mucinous stroma containing few tiny blood vessels were seen. Myxoid cells displayed a PAS-positive cytoplasm. The myxomatous matrix was alcianophilic at the pH of 2.50. To our knowledge, this is the first report of surgery, treatment, and histopathological study of myxoma in a bearded dragon (P. vitticeps). The sutures were also removed 12 days after the operation. No new growth of the mass was observed three months following the surgical procedures.
