Updated recommendations of the International Dengue Initiative expert group for CYD-TDV vaccine implementation in Latin America.

Dengue disease represents a large and growing global threat to public health, causing a significant burden to health systems of endemic countries. For countries considering vaccination as part of their Integrated Management Strategy for Prevention and Control of Dengue, the World Health Organization currently recommends the first licensed dengue vaccine, CYD-TDV for: individuals aged 9 years or above from populations with high transmission rates, based on either seroprevalence criteria or pre-vaccination screening strategies, and for persons with confirmed prior exposure to infection in moderate to lower transmission settings. This paper describes the main conclusions of the Sixth Meeting of the International Dengue Initiative (IDI) held in June 2018, following release of a new product label by the manufacturer, updated WHO-SAGE recommendations, additional scientific evidence on vaccine performance, and reports of experiences by implementing countries. Considerations were made regarding the need for improving the quality of epidemiological and surveillance data in the region to help define the convenience of either of the two vaccination strategies recommended by WHO-SAGE. Extensive discussion was dedicated to the pros and cons of implementing either of such strategies in Latin America. Although, in general, a seroprevalence-based approach was preferred in high transmission settings, when cost-effectivity is favorable pre-vaccination screening is a convenient alternative. Cost-effectiveness evaluations can assist with the decisions by public health authorities of whether to introduce a vaccine. Where implemented, vaccine introduction should be part of a public health strategy that includes the participation of multiple sectors of society, incorporating input from scientific societies, ministries of heath, and civil society, while ensuring a robust communication program.

[Yersinia enterocolitica: 1st isolation from meat products in Argentina]. / Yersinia enterocolitica: primeros aislamientos a partir de alimentos cárnicos en Argentina.

A search for Y. enterocolitica in fresh foods of animal origin has been carried out. Isolations were obtained from "chorizos" (thick fresh sausages), "salchichas" (slender fresh sausages), bovine and pig's tongues and caecum (Tables 1, 2). Enrichments were performed in saline phosphate buffer 0.067 M, pH 7.6 and nutrient broth with 0.5% glucose. Caecum and tongue samples were postenriched in 0.5% KOH. Subcultures were done in Salmonella-Shigella agar, MacConkey agar and eosin-methylene blue agar. Isolates were identified through biochemical, serological and lysotyping methods. The following biotypes (B), serotypes (O) and lysotypes (Lis) were isolated: from "chorizos", B2, 0:9, Lis X3 and B1, 0:7,8, Lis Xo. From "salchichas", B1, 0:5, Lis Xz. From bovine tongues, B1, 0:5, Lis Xz and B2, 0:9, Lis X3. From pig tongues, B1, 0:6, Lis Xz. From pig caecum, B1, 0:6, Lis Xz and B1, 0:5, Lis Xz. Serotype B2, 0:9, Lis X3 isolated from "chorizos" and bovine tongues has been only exceptionally found outside of human beings. Serotype B1, 0:6, Liz Xz was isolated from the tongue and caecum of one pig. From the caecum of another pig two serotypes, B1, 0:5, Liz Xz and B1, 0:6, Liz Xz, were isolated. In order to determine the importance of Y. entercolitica as etiologic agent of yersiniosis in Argentina, further studies on the frequency and distribution of this agent are recommended.

Yersinia enterocolitica: primeros aislamientos a partir de alimentos cárnicos en Argentina. / [Yersinia enterocolitica: 1st isolation from meat products in Argentina]

A search for Y. enterocolitica in fresh foods of animal origin has been carried out. Isolations were obtained from [quot ]chorizos[quot ] (thick fresh sausages), [quot ]salchichas[quot ] (slender fresh sausages), bovine and pigs tongues and caecum (Tables 1, 2). Enrichments were performed in saline phosphate buffer 0.067 M, pH 7.6 and nutrient broth with 0.5

glucose. Caecum and tongue samples were postenriched in 0.5

KOH. Subcultures were done in Salmonella-Shigella agar, MacConkey agar and eosin-methylene blue agar. Isolates were identified through biochemical, serological and lysotyping methods. The following biotypes (B), serotypes (O) and lysotypes (Lis) were isolated: from [quot ]chorizos[quot ], B2, 0:9, Lis X3 and B1, 0:7,8, Lis Xo. From [quot ]salchichas[quot ], B1, 0:5, Lis Xz. From bovine tongues, B1, 0:5, Lis Xz and B2, 0:9, Lis X3. From pig tongues, B1, 0:6, Lis Xz. From pig caecum, B1, 0:6, Lis Xz and B1, 0:5, Lis Xz. Serotype B2, 0:9, Lis X3 isolated from [quot ]chorizos[quot ] and bovine tongues has been only exceptionally found outside of human beings. Serotype B1, 0:6, Liz Xz was isolated from the tongue and caecum of one pig. From the caecum of another pig two serotypes, B1, 0:5, Liz Xz and B1, 0:6, Liz Xz, were isolated. In order to determine the importance of Y. entercolitica as etiologic agent of yersiniosis in Argentina, further studies on the frequency and distribution of this agent are recommended.