Dissipation of field-aligned currents in the topside ionosphere.

Field-aligned currents (FACs) are electric currents parallel to the geomagnetic field and connecting the Earth's magnetosphere to the high-latitude ionosphere. Part of the energy injected into the ionosphere by FACs is converted into kinetic energy of the surrounding plasma. Such a current dissipation is poorly investigated, mainly due to the high electrical conductivity and the small electric field strength expected in direction parallel to the geomagnetic field. However, previous results in literature have shown that parallel electric field is not null (and may be locally not negligible), and that parallel electrical conductivity is high but finite. Thus, dissipation of FACs may occur. In this work, for the first time, we show maps of power density dissipation features associated with FACs in the topside ionosphere of the Northern hemisphere. To this aim, we use a 6-year time series of data at one second cadence acquired by the European Space Agency's "Swarm A" satellite flying at an altitude of about 460 km. In particular, we use data from the Langmuir probe together with the FAC product provided by the Swarm team. The results obtained point out that dissipation of FACs, even if small when compared to that associated with horizontal currents flowing about 350 km lower, is not null and shows evident features co-located with electron temperature at the same altitude. In particular, power density dissipation features are enhanced mainly in the ionospheric regions where intense energy injection from the magnetosphere occurs. In addition, these features depend on geomagnetic activity, which quantifies the response of the Earth's environment to energetic forcing from magnetized plasma of solar origin.

Looking for a proxy of the ionospheric turbulence with Swarm data.

The present work focuses on the analysis of the scaling features of electron density fluctuations in the mid- and high-latitude topside ionosphere under different conditions of geomagnetic activity. The aim is to understand whether it is possible to identify a proxy that may provide information on the properties of electron density fluctuations and on the possible physical mechanisms at their origin, as for instance, turbulence phenomena. So, we selected about 4 years (April 2014-February 2018) of 1 Hz electron density measurements recorded on-board ESA Swarm A satellite. Using the Auroral Electrojet (AE) index, we identified two different geomagnetic conditions: quiet (AE < 50 nT) and active (AE > 300 nT). For both datasets, we evaluated the first- and second-order scaling exponents and an intermittency coefficient associated with the electron density fluctuations. Then, the joint probability distribution between each of these quantities and the rate of change of electron density index was also evaluated. We identified two families of plasma density fluctuations characterized by different mean values of both the scaling exponents and the considered ionospheric index, suggesting that different mechanisms (instabilities/turbulent processes) can be responsible for the observed scaling features. Furthermore, a clear different localization of the two families in the magnetic latitude-magnetic local time plane is found and its dependence on geomagnetic activity levels is analyzed. These results may well have a bearing about the capability of recognizing the turbulent character of irregularities using a typical ionospheric plasma irregularity index as a proxy.

A stress test to evaluate the usefulness of Akaike information criterion in short-term earthquake prediction.

Akaike information criterion (AIC) has been recently adopted to identify possible earthquake precursors in ionospheric total electron content (TEC). According to the authors of this methodology, their technique allows finding abrupt increases (positive breaks) in vertical TEC rate of change 25-80 min before the occurrence of large earthquakes, highlighting a promising implication of AIC method in Mw > 8 earthquakes alert strategies. Due to the relevance of this matter, a lively scientific debate ensued from these results. In this study, we carefully evaluate AIC method potentiality in searching earthquake TEC precursory signatures. We first investigate the dependence of the detected breaks number on the adjustable AIC method parameters. Then, we show that breaks occurrence clusters around specific local times and around moderate and high solar and geomagnetic activity. The outcome of this study is that AIC method is not concretely usable for issuing large earthquakes alerts.

Intracellular delivery of therapeutic antisense oligonucleotides targeting mRNA coding mitochondrial proteins by cell-penetrating peptides.

Cell-penetrating peptides are a promising therapeutic strategy for a wide variety of degenerative diseases, ageing, and cancer. Among the multitude of cell-penetrating peptides, PepFect14 has been preferentially used in our laboratory for oligonucleotide delivery into cells and in vivo mouse models. However, this activity has mainly been reported towards cytoplasm and nuclei, while the mentioned disorders have been linked to mitochondrial defects. Here, we report a library generated from a combinatorial covalent fusion of a mitochondrial-penetrating peptide, mtCPP1, and PepFect14 in order to deliver therapeutic biomolecules to influence mitochondrial protein expression. The non-covalent complexation of these peptides with oligonucleotides resulted in nano-complexes affecting biological functions in the cytoplasm and on mitochondria. This delivery system proved to efficiently target mitochondrial genes, providing a framework for the development of mitochondrial peptide-based oligonucleotide technologies with the potential to be used as a treatment for patients with mitochondrial disorders.