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1.
Int J Mol Med ; 18(5): 859-69, 2006 Nov.
Article in English | MEDLINE | ID: mdl-17016616

ABSTRACT

We report on the magnetic resonance spectroscopy (MRS) characterisation of different human meningiomas. Three histological subtypes of meningiomas (meningothelial, fibrous and oncocytic) were analysed both through in vivo and ex vivo MRS experiments. The ex vivo high-resolution magic angle spinning (HR-MAS) investigations, permitting an accurate description of the metabolic profile, are very helpful for the assignment of the resonances in vivo of human meningiomas and for the validation of the quantification procedure of in vivo MR spectra. By using one- and two-dimensional experiments, we were able to identify several metabolites in different histological subtypes of meningiomas. Our spectroscopic data confirmed the presence of the typical metabolites of these benign neoplasms and, at the same time, that meningomas with different morphological characteristics have different metabolic profiles, particularly regarding macromolecules and lipids. The ex vivo spectra allowed a better understanding and interpretation of the in vivo MR spectra, showing that the HR-MAS MRS technique could be a complementary method to strongly support the in vivo MR spectroscopy and increase its clinical potentiality.


Subject(s)
Biomarkers, Tumor/analysis , Magnetic Resonance Spectroscopy/methods , Meningeal Neoplasms/diagnosis , Meningioma/diagnosis , Aged , Female , Humans , Magnetic Resonance Imaging , Male , Meningeal Neoplasms/pathology , Meningioma/pathology , Middle Aged , Spectrum Analysis
3.
Ital J Biochem ; 53(4): 169-82, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15997910

ABSTRACT

The assumption that kidney tumors are characterized by high lipid content has directed our research towards the evaluation and comparison of lipids in healthy and neoplastic human renal tissues. This research appears to have marked importance owing to the role of lipids in cancer cell biochemistry. Nuclear Magnetic Resonance spectroscopy and chromatographic methods allow the full description of the lipidic profile typical of intact medulla, intact cortex, clear-cell renal carcinomas, chromophobe-cell renal carcinomas, oncocytomas and two samples of medulla infiltrated by a nephrocarcinoma. Significant differences among healthy, benign and malignant tissues were shown, and the results were discussed in relation to the different roles of each lipidic component and compared with the literature data.


Subject(s)
Kidney Neoplasms/chemistry , Kidney/chemistry , Lipids/analysis , Adenoma, Oxyphilic/chemistry , Carcinoma/chemistry , Cholesterol/analysis , Chromatography , Humans , Lipids/isolation & purification , Nuclear Magnetic Resonance, Biomolecular
4.
Ital J Biochem ; 52(2): 87-97, 2003 Jun.
Article in English | MEDLINE | ID: mdl-14677425

ABSTRACT

The spectroscopic characterisation of free carnosine and its coordination compounds with Cu(II), Zn(II) and Co(II) ions are discussed. Raman and IR studies on metal-carnosine systems have been performed, obtaining a relationship between the vibrational spectra and the structure of the predominant species formed. The biological activity of free carnosine and of its complexes is briefly considered.


Subject(s)
Carnosine/analysis , Cobalt/chemistry , Copper/chemistry , Zinc/chemistry , Animals , Carnosine/chemistry , Carnosine/metabolism , Free Radicals , Humans , Hydrogen-Ion Concentration , Models, Chemical , Protein Binding , Spectrophotometry, Infrared , Spectrum Analysis, Raman
5.
Anticancer Res ; 23(2B): 1541-8, 2003.
Article in English | MEDLINE | ID: mdl-12820421

ABSTRACT

BACKGROUND: The biochemical composition of human neoplastic and normal renal tissues. MATERIALS AND METHODS: Thirteen patients with nephrocarcinomas were examined: 24 samples, 13 from the nephrocarcinomas, 9 from the surrounding healthy parenchyma and 2 from the healthy cortex and medulla were extracted and analyzed by Magnetic Resonance Spectroscopy (MRS) and High Performance Liquid Chromatography (HPLC). RESULTS: MRS yielded information on renal osmolytes, whereas HPLC disclosed the amino acid pattern of tissue extracts. Significant biochemical differences were found between normal and pathological renal tissues: the osmolyte content decreases dramatically in nephrocarcinomas. CONCLUSION: We confirm that many osmolytes are present in the healthy kidney, with a different distribution between cortex and medulla, and can be considered markers of a physiological renal function. The marked decrease of these osmolytes is typical of cancer. A detailed knowledge of the biochemical composition of human renal tissues is required for the use of diagnostic methods, like in vivo MRS, the reliability of which is based on the detection of molecular markers. Useful information can also be obtained from the study of the amino acidic fraction.


Subject(s)
Carcinoma, Renal Cell/chemistry , Chromatography, High Pressure Liquid , Kidney Neoplasms/chemistry , Nuclear Magnetic Resonance, Biomolecular , 3-Hydroxybutyric Acid/analysis , Acetates/analysis , Adenocarcinoma, Clear Cell/chemistry , Adenocarcinoma, Clear Cell/pathology , Aged , Aged, 80 and over , Amino Acids/analysis , Betaine/analysis , Carcinoma, Renal Cell/pathology , Female , Glycerylphosphorylcholine/analysis , Humans , Inositol/analysis , Kidney Cortex/chemistry , Kidney Medulla/chemistry , Kidney Neoplasms/pathology , Male , Methylamines/analysis , Middle Aged , Phosphorylcholine/analysis , Succinates/analysis
6.
Ital J Biochem ; 52(4): 141-4, 2003 Dec.
Article in English | MEDLINE | ID: mdl-15141480

ABSTRACT

1H and 13C NMR spectroscopy was used to evaluate the degree of unsaturation and the cholesterol/cholesteryl ester ratio on the total lipid fractions obtained from human renal and cerebral tissues. The unsaturated/saturated fatty acid ratio was determined in the 13C NMR spectra from the ratio of the integrated areas of the resonances at 14.13 and 14.17 ppm assigned to the terminal methyl groups of saturated and unsaturated FA, respectively, and is validated by the traditional but time consuming gas-chromatographic analysis. Cholesteryl esters are easily discriminated in the total lipid fraction extracted from human tissues by means of the well-resolved component at 0.99 ppm (1H NMR spectra) of the resonance at about 1.00 ppm generally assigned to free cholesterol. The role of NMR spectroscopy in the study of lipidic biochemistry of human tissues is confirmed.


Subject(s)
Brain Chemistry , Kidney/chemistry , Lipids/analysis , Brain Neoplasms/chemistry , Humans , Kidney Neoplasms/chemistry , Nuclear Magnetic Resonance, Biomolecular
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