Microalgae and nano-cellulose composite produced via a co-culturing strategy for ammonia removal from the aqueous phase.

This study addresses the pressing need for sustainable bioremediation solutions to combat increasing pollution challenges in alignment with sustainability development goals. The research focuses on developing a co-culture approach involving microalgae and Komagataeibacter europaeus BCRC 14148 bacterium to create a biocomposite for efficient ammonia removal. Nanocellulose, produced by the bacterium, serves as a substrate for microalgae attachment. Optimization using specific growth media ratios resulted in biocomposite yields of 4.05 ± 0.16 g/L and 3.83 ± 0.13 g/L in HS medium with fructose and glucose, respectively. The optimal conditions include a 40:60 ratio of HS-F to TAP medium, 25 ℃ incubation, 6000 Lux light intensity, pH 5.5, and a 48-hour incubation period. When applied to wastewater treatment, the biocomposite demonstrated exceptional ammonium removal efficiency at 91.64 ± 1.27 %. This co-culture-derived biocomposite offers an eco-friendly, recyclable, and effective solution for sustainable environmental bioremediation.

Improved production of bacterial cellulose by Komagataeibacter europaeus employing fruit extract as carbon source.

Bacterial cellulose (BC) has attracted worldwide attention owing to its tremendous properties and versatile applications. BC has huge market demand, however; its production is still limited hence important to explore the economically and technically feasible bioprocess for its improved production. The current study is based on improving the bioprocess for BC production employing Komagataeibacter europeaus 14148. Physico-chemical parameters have been optimized e.g., initial pH, incubation temperature, incubation period, inoculum size, and carbon source for maximum BC production. The study employed crude and/or a defined carbon source in the production medium. Hestrin and Schramm (HS) medium was used for BC production with initial pH 5.5 at 30 °C after 7 days of incubation under static conditions. The yield of BC obtained from fruit juice extracted from orange, papaya, mango and banana were higher than other sugars employed. The maximum BC yield of 3.48 ± 0.16 g/L was obtained with papaya extract having 40 g/L reducing sugar concentration and 3.47 ± 0.05 g/L BC was obtained with orange extract having 40 g/L reducing sugar equivalent in the medium. BC yield was about three-fold higher than standard HS medium. Fruit extracts can be employed as sustainable and economic substrates for BC production to replace glucose and fructose. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-022-05451-y.

Removal of heavy metal vanadium from aqueous solution by nanocellulose produced from Komagataeibacter europaeus employing pineapple waste as carbon source.

Environmental concerns have taken a center stage in our lives driving the society towards biorefinery. Bioprocess development to produce valuable products utilizing waste has its own significance in circular bioeconomy and environmental sustainability. In the present study, production of bacterial cellulose using pineapple waste as carbon source by Komagataeibacter europaeus was undertaken and it was applied for removal of vanadium, a heavy metal which is generated as waste by semiconductors industry in Taiwan. Highest yield of bacterial cellulose (BC) e.i. 5.04 g/L was obtained with pineapple core hydrolysate (HS-PC) replacing glucose in HS medium. The vanadium adsorption capacity by BC produced by HS medium was 5.24 mg/g BC at pH 4 and 2.85 mg/g BC was observed on PCH medium. BC was characterised via SEM, FTIR and XRD.

Bioprospecting of marine microalgae from Kaohsiung Seacoast for lutein and lipid production.

A bioprospecting study was conducted from Seawater samples collected at Kaohsiung Seacoast, Taiwan. The current research was aimed to isolate potential lutein-producing strain, evaluate and optimize the best cultivation mode, lutein accumulation stage, lutein-extraction method, and condition to recover maximum lutein (main product) and lipid (byproduct). Biorefinery is the latest approach worldwide to extract multi-products for cost-effectiveness. Selected isolate among several isolates, identified as Chlorella sorokiniana Kh12 and exploited under biorefinery concept for lutein and lipid extraction. Kh12 cultivated under mixotrophy: 2X-(HT)-9k yielded maximum biomass (3.46 g L-1) and lutein (13.69 mg g-1) which is among the higher yields reported so far. Among various tested solvents, methanol was the best extractor. Bead milling was most effective to disrupt algal cell walls, seven minutes of milling was best for maximum lutein (7.56 mg g-1) extraction. Kh12 could be a promising candidate for commercial lutein and lipid co-production based on the outcome.

Developments in bioprocess for bacterial cellulose production.

Bacterial cellulose (BC) represents a novel bio-origin nonomaterial with its unique properties having diverse applications. Increased market demand and low yield are the major reason for its higher cost. Bacteria belonging to Komagataeibacter sp are the most exploited ones for BC production. Development of a cost-effective bioprocess for higher BC production is desirable. Though static fermentation modes have been majorly employed for BC production using tray fermenters, agitated mode has also been employed successfully with air-lift fermenters as well as stirred tank reactors. Bioprocess advances in recent years has led BC production to an upper level; however, challenges of aeration requirement and labor cost towards the higher end is associated with static cultivation at large scale. We have discussed the bioprocess development for BC production in recent years along with the challenges associated and the path forward.

Novel application of microalgae platform for biodesalination process: A review.

Freshwater demand is rising worldwide due to largely increasing population and industrialization. Latest focus is to explore the Ocean and saline effluent from industries to produce freshwater in a sustainable way via algal desalination. Current physicochemical desalination technology is not only an energy-intensive and expensive process but also gives severe environmental impact from brine and GHGs emissions. Therefore, it is neither environmentally-friendly nor feasible to countries with limited resources. Biodesalination could be an attractive technology with recent breakthroughs in algal bioprocess with fast growth rate under highly saline conditions to effectively remove salts optimally 50-67% from saline water. Algal desalination mainly occurs through biosorption and bioaccumulation which governs by biotic and abiotic factors e.g., strain, temperature, pH, light and nutrients etc. This review provides a current scenario of this novel technology by an in-depth assessment of technological advancement, social impact, possible risks and scope for policy implications.