ABSTRACT
Objective: To evaluate efficacy of vaccination against tick-born encephalitis by determination of specific antibody titer. Materials and Methods. 224 vaccinated residents (77 females, 147 males) of Selenge aimag with mean age of 33.1(6- 60), 20 worker of Ulaanbaatar with mean age of 36 (23-53) were enrolled to the study. We used Avidity determination of antibodies againt TBE Virus ELISA (Ig G) manufactured by the EUROIMMUN.Result:At 2-6 months after first dose RAI% was in low (29.4-32%), at 1 month after second dose RAI% was in equivocal (53.9%-55.6%), at 6 months after third vaccination RAI% was in high (72.5%-79.8%) avidity antibodies in two groups. That mean RAI% is increasing depend from repetition doses in both schedule. These differences were statistically significant for all post vaccination evaluation days (60,90; p0.05) level of RAI% in 6 months after second dose with compared two schedule. But it getting high level of RAI% was developed in short time (before 90 days) with the accelerated schedule than conventional schedule.Antibody titer of 2-200 RU/ ml were observed in all attendants of Selenge aimag. But only 34% of them show a protective titer. In details 62.8% of people vaccinated in 2002-2004, 55.8% of people vaccinated in 2005-2007 and 50.2% of people vaccinated in 2007-2009 year have demonstrated a protective titer. Only 21.7% and 13.4% of people vaccinated by rapid scheme and people received only first dose, respectively, have a protective immunity against TBE.Conclusion:1. The level of RAI% is increasing depends from repetition doses of vaccine TBE with accelerated and conventional schedule.2. The high level of RAI% is getting at 6 months after second dose with accelerated and conventional schedule.3. Complete dose of TBE vaccine develop a better action for establishment of specific protective immunity.
ABSTRACT
BACKGROUND Every year 3-5 million cases have reported during human influenza epidemics and Disease 300 000-500 000 of them die. People who aged over 65 and under 2 year counted as a 'School of BioMeJicine. Health Sciences high risk group to influenza. When mix red blood cells and influenza virus, it shows llemaglutination reaction, which is caused by binding of influenza virus surface molecule hemagglutinin with red blood cell (RBC) receptor with syalic acid residues. This reaction is used traditionally as main and effective assay fw infl*wn*a virus detection. PURPOSE • To test local strains of A(H1N1), A(H3N2), A(II5N1) and B type influenza virus with human, guinea pig, chicken and horse RBCs • To choose the most sensitive animal RBC for detection of different influenza viruses METHODS We have used isolates of A/Ulaanbaatar/1 N85/07 (H,N,), /J| i laanbaatar 1654 II (H,N,), B/Ulaanbaalar/1877/07 isolated in the Respiratory Virology Laboratory, National Center for Communicable Diseases, A/Wooper Swan Mongolia 65 <16 (H5\ I) isolated in Central Veterinary Laboratory with human 01. horse, chicken and guinea pig RBCs. The subtyping of the Human influenza viruses have been performed with rt-PCR with specific primers. We used protocol of CDC, USA to isolate human influenza strains in MIX K cell culture, to determine strains with Hemagglutination test. RESULTS Human Influenza A(H IN I ).A(H3N2)and B type viruses are more agglutinable with to guinea pig RBCs Avian influenza A(H5N1) virus is more agglutinable with human 01 and horse RBC. CONCLUSIONS Guinea pig RBCs is the most effective for daily human influenza virus detection procedures and using horse and guinea pig RBC together is reasonable tor avian influenza suspected cases.
