Effects of single and repeated bouts of flywheel exercise on jump performance and muscle damage in athletes and non athletes.

BACKGROUND: Although recent studies have investigated the effects of flywheel (FW) training on muscle function, the effects of transient FW exercise on jump performance in athletes are unknown. This study examined the effects of single and repeated bouts of FW squat exercises on jump performance and muscle damage in male collegiate basketball players. METHODS: The participants were 10 healthy college-age men (nonathletes) and 11 male basketball players (athletes). The intervention involved 100 squat exercises (10 repetitions × 10 sets) using an FW device. To examine the repeated-bout effects, the protocol was conducted again after a 2-week interval. Squat jumps, countermovement jumps, drop jumps, and rebound jumps were evaluated as jump performance, while isometric maximal voluntary contraction (MVC) torque in knee extension, muscle soreness, range of motion, thigh circumference, muscle thickness, and echo intensity were evaluated as markers of muscle damage. Measurements were taken at baseline, immediately after exercise, 24 h later, and 72 h later. RESULTS: The jump performance of nonathletes decreased after exercise (p < 0.05), while that of the athletes did not. The results were similar for muscle soreness. MVC torque decreased significantly after the first exercise in both groups (p < 0.05) and was significantly lower in the nonathletes versus athletes. Significant repeated-bout effects were found for muscle soreness in nonathletes but not athletes. CONCLUSIONS: These results suggest that a single bout of FW exercise reduces jump performance in male nonathletes but not basketball players.

Home-based high-intensity interval training improves cardiorespiratory fitness: a systematic review and meta-analysis.

BACKGROUND: High-intensity interval training (HIIT) is an effective methods to improve maximal oxygen uptake. However, there is no definitive conclusion about the specific effectiveness of home-based HIIT. This review investigated the effects of home-based HIIT on cardiorespiratory fitness in a systematic review and meta-analysis. METHODS: Four electronic databases were searched (PubMed, Cochran database, Web of Science, Igaku Chuo Zasshi) for studies through March 25, 2023. Eligibility criteria include randomized controlled trials of home-based HIIT in adult people regardless disease or handicaped. Comparisons were made between non-exercise controls, laboratory-based HIIT, and moderate-intensity continuous training (MICT). The primary outcome was defined as cardiorespiratory fitness and the secondary outcome was defined as patient-reported outcomes. The standardized mean difference (SMD) with 95% confidence intervals (CIs) was calculated for quantitative indices. The random-effect model was used as the pooling method. RESULTS: Two hundred seven studies were identified, and 15 satisfied the inclusion criteria. The meta-analysis for cardiorespiratory fitness showed superiority of home-based HIIT to non-exercise controls (SMD 0.61, 95% CI: 0.21, 1.02). However, no significant difference in cardiorespiratory fitness was observed between home-based HIIT and lab-based HIIT (SMD: -0.35, 95%CI: -0.73, 0.03). Also, no significant difference was observed between the home-based HIIT and MICT (SMD 0.34, 95% CI: -0.05, 0.73). CONCLUSION: These results indicated that home-based HIIT was an effective intervention for improving cardiorespiratory fitness in healthy adults and patients. Importantly, this review found no significant differences in cardiorespiratory fitness between home-based HIIT and the group of laboratory HIIT and MICT, highlighting its comparable effectiveness and potential as a practical and valuable exercise intervention.

Eicosapentaenoic Acid and Medium-Chain Triacylglycerol Structured Lipids Improve Endurance Performance.

PURPOSE: The effects of intake of STGs containing esterified eicosapentaenoic acid (EPA) and medium-chain triglycerides (MCTs) on cardiorespiratory endurance have not yet been reported. This study aimed to examine the efficacy of interesterified structured lipids EPA and MCTs on cardiorespiratory endurance. METHODS: This 8-week randomized double-blind placebo-controlled parallel-group study involved 19 healthy men. The participants were randomly assigned to a group that received interesterified structured lipids EPA and MCTs (STG group, 9 participants) or a group receiving a PM of EPA and MCTs (PM group, 10 participants). The outcome measures were time to exhaustion (TTE) and time to reach the anaerobic threshold in the peak oxygen uptake (VO2peak) test, VO2peak, and anaerobic threshold. RESULTS: The increase in TTE in the VO2peak test after the intervention period compared with before the intervention period was significantly greater in the STG group (53 ± 53 s) than in the PM group (-10 ± 63 s; p < 0.05). Similarly, the increase in time to reach the anaerobic threshold was significantly greater in the STG group (82 ± 55 s) than in the PM group (-26 ± 52 s; p < 0.001). CONCLUSION: This study demonstrated that the consumption of interesterified structured lipids EPA and MCTs improved endurance in humans.

Pedal cadence does not affect muscle damage to eccentric cycling performed at similar mechanical work.

Purpose: This study aimed to investigate muscle damage when performing equal mechanical work of fast and slow pedaling speed by eccentric muscle actions (ECCs) cycling. Methods: Nineteen young men [mean ± standard deviation (SD) age: 21.0 ± 2.2 years; height: 172.7 ± 5.9 cm; and body mass: 70.2 ± 10.5 kg] performed maximal effort of ECCs cycling exercise with fast speed (Fast) and slow speed trials (Slow). First, subjects performed the Fast for 5 min by one leg. Second, Slow performed until the total mechanical work was equal to that generated during Fast other one leg. Changes in maximal voluntary isometric contraction (MVC) torque of knee extension, isokinetic pedaling peak torque (IPT), range of motion (ROM), muscle soreness, thigh circumference, muscle echo intensity, and muscle stiffness were assessed before exercise, and immediately after exercise, and 1 and 4 days after exercise. Results: Exercise time was observed in the Slow (1422.0 ± 330.0 s) longer than Fast (300.0 ± 0.0 s). However, a significant difference was not observed in total work (Fast:214.8 ± 42.4 J/kg, Slow: 214.3 ± 42.2 J/kg). A significant interaction effect was not observed in peak values of MVC torque (Fast:1.7 ± 0.4 Nm/kg, Slow: 1.8 ± 0.5 Nm/kg), IPT, muscle soreness (Fast:4.3 ± 1.6 cm, Slow: 4.7 ± 2.9 cm). In addition, ROM, circumference, muscle thickness, muscle echo intensity, and muscle stiffness also showed no significant interaction. Conclusion: The magnitude of muscle damage is similar for ECCs cycling with equal work regardless of velocity.

Omega-3 fatty acids enhance the beneficial effect of BCAA supplementation on muscle function following eccentric contractions.

Background: This study investigated the combined effect of branched-chain amino acids (BCAA) and fish oil (FO) on muscle damage caused by eccentric contractions (ECCs) of the elbow flexors, with a special focus on muscular function. Methods: Twenty-nine untrained male participants were enrolled in this double-blind, placebo-controlled, parallel study. The participants were randomly assigned to the placebo (PL) group (n = 9), BCAA supplement group (n = 10), and BCAA+FO supplement group (n = 10). The BCAA+FO group consumed eicosapentaenoic acid (EPA) 600 mg and docosahexaenoic acid (DHA) 260 mg per day for 8 weeks, while the BCAA and BCAA+FO groups consumed 9.6 g per day for 3 days prior to and until 5 days after ECCs. Participants performed six sets of 10 ECCs at 100% maximal voluntary contraction (MVC) using dumbbells. Changes in MVC torque, range of motion (ROM), muscle soreness using visual analog scales, upper circumference, muscle thickness, echo intensity, and serum creatine kinase (CK) were assessed before, immediately after, and 1, 2, 3, and 5 days after ECCs. Results: The MVC torque was significantly higher in the BCAA+FO group than in the PL group immediately after ECCs (p < 0.05) but not in the BCAA group. Both BCAA and BCAA+FO groups showed greater ROM and lower muscle soreness than the PL group (p < 0.05). CK was significantly lower in the BCAA group than in the PL group at 5 days after ECCs (p < 0.05). Conclusions: This study reveals that supplementation with BCAA and FO may favorably impact immediate recovery of peak torque production. Alternatively, in comparison to PL group, BCAA supplementation favorably reduces creatine kinase.

Eccentric exercise causes delayed sensory nerve conduction velocity but no repeated bout effect in the flexor pollicis brevis muscles.

PURPOSE: This study was aimed at investigating the effect of eccentric contractions (ECCs) of flexor pollicis brevis muscles (FPBMs) on motor and sensory nerve functions as well as the ipsilateral repeated bout effect (IL-RBE) and contralateral (CL)-RBE of motor and sensory nerve conduction velocities following ECCs. METHODS: Thirty-two young healthy men (age: 19.6 ± 0.2 years, height: 173.2 ± 1.2 cm, body mass: 69.7 ± 1.9 kg) performed two bouts of ECCs. During the first ECCs bout (ECCs-1), all participants performed 100 ECCs with 1 hand; for the second bout, 3 groups (2 weeks [W]: n = 11, 4W: n = 10, 8W: n = 11) performed ECCs with both hands 2, 4, or 8 weeks after ECCs-1. The maximal voluntary isometric contraction (MVC), range of motion (ROM), visual analog scale for pain (VAS), motor and sensory nerve conduction velocities were measured before, immediately after, and 1, 2, 3, and 5 days after ECCs. RESULTS: ECCs-1 decreased the MVC, limited the ROM, developed VAS, and decreased the motor and sensory nerve conduction velocities compared to non-exercise hand (p < 0.05). The repeated bout effect was observed in the ROM for IL-RBE in 2W and 4W, VAS for IL-RBE in 2 W, and ROM and VAS for CL-RBE in 2W (p < 0.05). However, RBEs of MVC and motor and sensory nerve conduction velocities were not observed, and no differences were confirmed depending on the interval. CONCLUSION: In the present study, ECCs of the FPBM caused a sensory nerve dysfunction, while IL- or CL-RBE was not observed.

4-week eicosapentaenoic acid-rich fish oil supplementation partially protects muscular damage following eccentric contractions.

BACKGROUND: We previously showed 8-week of fish oil supplementation attenuated muscle damage. However, the effect of a shorter period of fish oil supplementation is unclear. The present study investigated the effect of fish oil, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for 4 weeks on muscular damage caused by eccentric contractions (ECCs) of the elbow flexors. METHODS: Twenty-two untrained men were recruited in this double-blind, placebo-controlled, parallel design study and the subjects were randomly assigned to the EPA and DHA group (EPA and DHA, n = 11) and placebo group (PL, n = 11). They consumed either EPA 600 mg and DHA 260 mg per day or placebo supplement for 4 weeks prior to exercise. Subjects performed 60 ECCs at 100 % maximal voluntary contraction (MVC) using a dumbbell. Changes in MVC torque, range of motion (ROM), upper arm circumference, muscle soreness, echo intensity, muscle thickness, serum creatine kinase (CK), and interleukin-6 (IL-6) were assessed before exercise; immediately after exercise; and 1, 2, 3, and 5 days after exercise. RESULTS: ROM was significantly higher in the EPA and DHA group than in the PL group immediately after performing ECCs (p < 0.05). No differences between groups were observed in terms of MVC torque, upper arm circumference, muscle soreness, echo intensity, and thickness. A significant difference was observed in serum CK 3 days after ECCs (p < 0.05). CONCLUSIONS: We concluded that shorter period EPA and DHA supplementation benefits joint flexibility and protection of muscle fiber following ECCs.

Low Dose of ß-Hydroxy-ß-Methylbutyrate (HMB) Alleviates Muscle Strength Loss and Limited Joint Flexibility following Eccentric Contractions.

Objective: The optimal dose of ß-hydroxy-ß-methylbutyrate (HMB) required for decreasing muscle damage remains unknown. Therefore, this study investigated the supplemental effect of low-dose HMB on muscle damage following eccentric contractions (ECCs) of human elbow flexors.Methods: Twenty untrained men (aged 20-26; height, 169.4 ± 5.7 cm; weight, 68.2 ± 11.3 kg; body mass index, 23.7 ± 3.3) completed the double-blind, placebo-controlled, parallel design study. The subjects were randomly assigned to the ingestion of HMB supplement (HMB, n = 10) or placebo group (PL, n = 10). After the subjects of 1.5 g HMB or placebo pills per day for 2 weeks, they performed six sets of 10 ECCs at 100% maximal voluntary contraction (MVC) using dumbbell. Changes in MVC torque, range of motion (ROM), upper arm circumference, muscle soreness, and muscle stiffness were assessed before, immediately after, 1, 2, 3, 5 days after exercise.Results: MVC torque was significantly higher in the HMB group than in the PL group immediately after (HMB, -56.8%; PL, -67.1%) as well as 3 (HMB: -25.5%, PL: -48.7%) and 5 (HMB: -22.5%, PL: -44.0%) days after performing ECCs (p < 0.05). Additionally, ROM was significantly higher in the HMB group than in the PL group immediately after (HMB, -29.8%; PL, -50.5%) and 5 (HMB: -26.1%, PL: -43.3%) days after performing ECCs (p < 0.05). No between-group differences were observed in other muscle damage markers.Conclusion: The low-dose HMB supplementation demonstrated a moderate overall impact and played a beneficial role in muscle dysfunction and joint flexibility following ECCs.

Fast-Velocity Eccentric Cycling Exercise Causes Greater Muscle Damage Than Slow Eccentric Cycling.

This study aims to investigate muscle damage occurring in the early and recovery phases after fast-velocity and slow-velocity eccentric cycling. Eleven untrained men (age, 20.0 ± 1.7 years; height, 171.3 ± 6.8 cm; weight, 61.8 ± 7.7 kg; and %body fat, 13.2 ± 2.9%) performed slow-velocity maximal isokinetic eccentric cycling (slow-velocity; 30°/s) with one leg and fast-velocity (fast-velocity; 210°/s) isokinetic eccentric cycling with the other leg. Changes in maximal voluntary isokinetic concentric contraction (MVCC) torque at velocities of 30 and 210°/s, range of motion (ROM), and muscle soreness were assessed by pressure using a digital muscle stiffness instrument; thigh circumference, muscle echo intensity, and muscle stiffness were assessed before exercise, and immediately after exercise, 1 day, and 4 days after exercise. Comparing with the results obtained for slow-velocity cycling (post: 215.9 ± 32.3 Nm, day 1: 192.9 ± 47.4 Nm, day 4: 184.3 ± 47.2 Nm) and before exercise, MVCC after fast-velocity cycling significantly decreased at immediately (160.4 ± 43.5 Nm), 1 day (143.6 ± 54.1 Nm), and 4 days (150.1 ± 44.5 Nm) after exercise (p < 0.05). Significant increase in muscle soreness for vastus lateralis was observed after fast-velocity cycling (41.2 ± 16.9 mm) compared with slow-velocity cycling (23.7 ± 12.2 mm) 4 days after exercise (p < 0.05). However, no significant difference in muscle soreness was observed for rectus femoris and vastus medialis at any time points after exercise. In addition, no significant differences were observed in the ROM, thigh circumference, muscle echo intensity, and muscle stiffness. In conclusion, fast-velocity eccentric cycling causes a decrease in muscle strength and an increase in soreness as compared to slow-velocity eccentric cycling.

Muscular endurance and muscle metabolic responses to 8 weeks of omega-3 polyunsaturated fatty acids supplementation.

BACKGROUND: It has been well known that exercise training improves muscular endurance; however, whether nutritional strategies can be used to enhance muscular endurance remains unclear. Herein, we tested the hypothesis that 8 weeks of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation, known to promote oxygen availability and lipid metabolism, would attenuate muscular fatigue caused by numerous muscle contractions. METHODS: Nineteen healthy men were randomly assigned to a placebo group (n = 9) and fish oil group (n = 10) in a double-blind fashion. The fish oil group consumed EPA-rich fish oil that contains 600-mg EPA and 260-mg DHA per day for 8 weeks. The placebo group received matching capsules for the same duration of time. After the 8-week intervention, subjects performed muscular endurance test that was repeated knee extensions with weights equal to 40% of the subject's body weight. RESULTS: Maximal repetitions to exhaustion were recorded. In addition, maximum isometric voluntary muscle contraction (MVC), muscle metabolism using near-infrared spectroscopy, and blood lactate were measured during the test. Subjects in both groups reached exhaustion after the muscular endurance test, while the maximal repetitions did not differ between the groups. Similarly, there is no significant difference in oxygen saturation in muscle tissue (StO2), an index of muscle oxygen availability, between the groups. Also, MVC and blood lactate did not change between groups. CONCLUSION: In conclusion, the present study provided evidence that muscle fatigue caused by knee extensions cannot be attenuated by EPA and DHA supplementation in healthy subjects.

Sitting-induced Endothelial Dysfunction Is Prevented in Endurance-trained Individuals.

PURPOSE: Prolonged sitting impairs leg endothelial function, which seems to be mediated by a sustained reduction in blood flow-induced shear stress. However, whether regular endurance training is effective in preventing sitting-induced leg endothelial dysfunction remains largely unknown. Herein, we tested the hypothesis that sitting-induced leg endothelial dysfunction is prevented in high endurance-trained individuals. METHODS: The endurance-trained group comprised 10 male collegiate cyclists, and the untrained group comprised nine men with no regular endurance training. Peak oxygen uptake (V˙O2peak) was initially determined in all participants using incremental exercise test (37.9 ± 4.7 mL·min·kg in the untrained group versus 60.8 ± 3.6 mL·min·kg in the endurance-trained group). At second visit, the popliteal artery flow-mediated dilation (%FMD) was assessed before and after a 3-h sitting period. During the sitting period, the popliteal artery diameter and blood velocity were measured every hour. RESULTS: The popliteal artery blood flow and shear rate were significantly and similarly reduced during the sitting period in both groups (P < 0.001). In a 3-h sitting, a significant impairment in popliteal artery %FMD was observed in the untrained group (P = 0.003), but it was prevented in the endurance-trained group (P < 0.196). CONCLUSIONS: In conclusion, the present study revealed that sitting-induced leg endothelial dysfunction is preventable in endurance-trained individuals.

Eccentric contraction-induced muscle damage in human flexor pollicis brevis is accompanied by impairment of motor nerve.

BACKGROUND: Eccentric contractions (ECCs) cause muscle damage. In addition, we showed that ECCs induce nerve dysfunction and damage with rats and human. PURPOSE: We aimed to evaluate motor nerve conduction velocity (MCV) for flexor pollicis brevis muscle (FPBM) after ECCs. METHODS: Twelve men (years, 19.8 ± 1.7 years; height, 172.4 ± 7.0 cm; weight, 64.0 ± 8.6 kg) performed maximal 100 ECCs on their FPBM of non-dominant hands with torque dynamometer. The dominant hands were control (CON). Maximal voluntary contraction (MVC), range of motion (ROM), DOMS, and MCV were assessed before, immediately post, and 1, 2, and 5 days after ECCs. MCV was calculated as the distance by stimulation divided by the latencies of the waveforms generated. Values were statistically analyzed by two-way ANOVA, and the significance level was set at P < .05. RESULTS: Decreases in MVC immediately (-32.9%) to 5 days after ECCs were significantly greater (P < .05) than for the CON group. ROM showed a significant decrease immediately (-21.6%) after ECCs compared with before ECCs and CON group (P < .05). DOMS after ECCs increased at 1 and 2 days (5.0 cm) after ECCs compared with before ECCs and CON (P < .05). Also, MCV after ECCs delayed significantly from immediately (-36.4%), 1, 2, and 5 days after ECCs compared with CON (P < .05), while no significant change in M-wave amplitude was observed over time for both ECCs and CON. CONCLUSION: The present study showed that ECCs of the FPBM cause a significant delay in MCV of median nerve.

Supplementation of eicosapentaenoic acid-rich fish oil attenuates muscle stiffness after eccentric contractions of human elbow flexors.

BACKGROUND: This study aimed to investigate the effect of supplementation of fish oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the damage of the biceps brachii after eccentric contractions (ECCs) of the elbow flexors, particularly focusing on muscle stiffness. METHODS: Sixteen men were included in this double-blind, placebo-controlled, parallel design study and the participants were randomly assigned to the EPA and DHA supplement group (EPA, n = 8) and placebo group (PL, n = 8). They consumed either EPA 600 mg and DHA 260 mg per day or placebo supplement for 8 weeks prior to exercise. Moreover, they performed six sets of 10 ECCs at 100% maximal voluntary contraction (MVC) using a dumbbell. Changes in MVC torque, range of motion (ROM), upper arm circumference, muscle soreness, muscle echo intensity, and muscle stiffness were assessed before exercise; immediately after exercise; and 1, 2, and 5 days after exercise. RESULTS: MVC torque and ROM were significantly higher in the EPA group than in the PL group after ECCs (p < 0.05). Muscle soreness, upper arm circumference, and muscle echo intensity were significantly higher in the PL group than in the EPA group after ECCs (p < 0.05). In addition, muscle stiffness at 150° was significantly higher in the PL group than in the EPA group immediately after ECCs (p < 0.05). CONCLUSION: The present study showed that EPA and DHA supplementation has a positive role in inhibiting muscle stiffness after ECCs. TRIAL REGISTRATION: This trial (UMIN000028165) was registered on 10th/July/2017.

Muscular recruitment is associated with muscular function and swelling following eccentric contractions of human elbow flexors.

BACKGROUND: This study investigated the relationship between muscle fiber recruitment and the magnitude of muscle damage by isotonic eccentric contractions (ECCs). METHODS: Ten healthy men (age: 27.2±6.0 y, height: 174. 0±5.3 cm, body mass 70.1±2.1 kg) were recruited in this study. Subjects performed 30 ECCs of the elbow flexors using a dumbbell adjusted to 40% and 80% one repetition maximum of each individual. The dependent variables maximal voluntary isometric contraction (MVC) torque, Range of Motion (ROM), delayed onset muscle soreness (DOMS), cross-sectional area (CSA) and transverse relaxation time (T2) of magnetic resonance imaging (MRI) were measured immediately before, immediately after and 1, 3, and 5 days after each exercise. RESULTS: The decreased MVC and limited ROM were significantly greater for 80% than that 40% (P<0.05) at immediately after ECCs. However, no significant difference between 40% and 80% was found for DOMS. CSA at 5 days after ECCs was significantly higher 80% (P<0.05) than 40% (P<0.05). No significant changes in post T2 (acute T2) was found for 40%, but an increased acute T2 was observed in 80% (P<0.05). We found a significant correlation between the change in T2 at immediately after and MVC at immediately after (r=0.77, P<0.05). In addition, a significant correlation between the change in T2 at immediately after and change in CSA at 3 days after (r=-0.83, P<0.05) was found. CONCLUSIONS: We conclude that the muscle strength loss and swelling following ECCs are related to the muscle fiber recruitment.

Two and Four Weeks of ß-Hydroxy-ß-Methylbutyrate (HMB) Supplementations Reduce Muscle Damage Following Eccentric Contractions.

OBJECTIVE: This study investigated the effect of ß-hydroxy-ß-methylbutyrate (HMB) supplementation for either 2 or 4 weeks on the muscle damage after elbow flexors after eccentric contractions (ECCs). METHODS: Twenty-eight untrained men were completed the double-blind, placebo-controlled, parallel design study. The subjects were randomly assigned to the ingestion of HMB supplement for 2 weeks (HMB 2-week, n = 10), for 4 weeks (HMB 4-week, n = 10), or a placebo group (PL, n = 8). Subjects of HMB 2-week and HMB 4-week consumed 3 g HMB per day, and they performed 6 sets of 10 ECCs at 100% maximal voluntary contraction (MVC) using a dumbbell. Changes in MVC torque, range of motion (ROM), upper arm circumference, muscle soreness, and muscle stiffness were assessed before, immediately after, and 1, 2, and 5 days after exercise. RESULTS: MVC torque and ROM were significantly higher in the HMB 2-week and HMB 4-week groups than in the PL group after ECCs (p < 0.05). The upper arm circumference was significantly smaller in the HMB 2- and 4-week groups than in the PL group after ECCs (p < 0.05). In addition, muscle stiffness at 150° was significantly lower in the HMB 2- and 4-week groups than in the PL group at immediately after ECCs (p < 0.05). However, there was no difference in all outcomes between HMB 2-week and HMB 4-week. CONCLUSION: We concluded that more than 2 weeks of HMB supplementation has a positive role for untrained subjects to prevent the muscle damage after ECCs.

Automated stopped-in-dual-loop flow analysis system for catalytic determination of vanadium in drinking water.

An automated stopped-in-dual-loop flow analysis (SIDL-FA) system is proposed for the determination of vanadium in drinking water. The chemistry is based on the vanadium-catalyzed oxidation reaction of p-anisidine by bromate in the presence of Tiron as an activator to produce a dye (lambda(max)=510 nm). A SIDL-FA system basically consists of a selection valve, three pumps (one is for delivering of standard/sample, and others are for reagents), two six-way injection valves, a spectrophotometric detector and a data acquisition device. A 100-microL coiled loop around a heated device is fitted onto each six-way injection valve. A well-mixed solution containing reagents and standard/sample is loaded into the first loop on a six-way valve, and then the same solution is loaded into the second loop on another six-way valve. The solutions are isolated by switching these two six-way valves, so that the catalytic reaction can be promoted. The net waste can be zero in this stage, because all pumps are turned off. Then each resulting solution is dispensed to the detector with suitable time lag. A touchscreen controller is developed to automatically carry out the original SIDL-FA protocol. The proposed SIDL-FA method allows vanadium to be quantified in the range of 0.1-2 microg L(-1) and is applied to the determination of vanadium in drinking water samples.