Exploring the diversity of virulence genes in the Magnaporthe population infecting millets and rice in India.

Blast pathogen, Magnaporthe spp., that infects ancient millet crops such pearl millet, finger millet, foxtail millet, barnyard millet, and rice was isolated from different locations of blast hotspots in India using single spore isolation technique and 136 pure isolates were established. Numerous growth characteristics were captured via morphogenesis analysis. Among the 10 investigated virulent genes, we could amplify MPS1 (TTK Protein Kinase) and Mlc (Myosin Regulatory Light Chain edc4) in majority of tested isolates, regardless of the crop and region where they were collected, indicating that these may be crucial for their virulence. Additionally, among the four avirulence (Avr) genes studied, Avr-Pizt had the highest frequency of occurrence, followed by Avr-Pia. It is noteworthy to mention that Avr-Pik was present in the least number of isolates (9) and was completely absent from the blast isolates from finger millet, foxtail millet, and barnyard millet. A comparison at the molecular level between virulent and avirulent isolates indicated observably large variation both across (44%) and within (56%) them. The 136 Magnaporthe spp isolates were divided into four groups using molecular markers. Regardless of their geographic distribution, host plants, or tissues affected, the data indicate that the prevalence of numerous pathotypes and virulence factors at the field level, which may lead to a high degree of pathogenic variation. This research could be used for the strategic deployment of resistant genes to develop blast disease-resistant cultivars in rice, pearl millet, finger millet, foxtail millet, and barnyard millet.

A Comparative Evaluation of Shear Bond Strength of Seventh- and Eighth-Generation Self-etch Dentin Bonding Agents in Primary Teeth: An In Vitro Study.

AIM: To compare the shear bond strength of seventh- and eight-generation self-etch dentin bonding agents in primary teeth using universal testing machine. MATERIALS AND METHODS: Forty extracted sound human primary molars were collected and randomly divided into two groups of 20 sample each. Shear bond strength of seventh-generation bonding agent [ADPER Single Bond Universal, (group II)] and eighth-generation bonding agent [Futurabond DC, VOCO Germany, (group I)] were calculated by using Universal Testing Machine (PTC/O83/ME, INSTRON, USA) and expressed in megapascals (MPa). Recorded data were compiled and subjected to statistical analysis using Student's t test. RESULTS: The mean shear bond strength of group I (eighth-generation dentin bonding agent) and group II (seventh-generation dentin bonding agent) ranged from 22.10 to 37.10 MPa and 19.80 to 30.30 MPa, respectively Student's t test showed significantly different and higher (8.7%) shear bond strength in group I when compared to group II (p value = 0.017). CONCLUSION: Shear bond strength of eighth-generation dentin bonding agent (Futurabond DC) was better than seventh-generation dentin bonding agent (Adper Single Bond Universal). CLINICAL SIGNIFICANCE: An effective bond to tooth would reduce marginal microleakage, bacterial penetration, postoperative sensitivity, possibility of pulpal inflammation, and preserve tooth structure by allowing minimal cavity preparation. HOW TO CITE THIS ARTICLE: Mishra A, Koul M, Upadhyay VK, et al. A Comparative Evaluation of Shear Bond Strength of Seventh- and Eighth-generation Self-etch Dentin Bonding Agents in Primary Teeth: An In Vitro Study. Int J Clin Pediatr Dent 2020;13(3):225-229.

A rare case of three single rooted permanent second molars: A diagnosis with cone beam computed tomography (CBCT).

The aim of this article is to present a case with an unusual morphology of maxillary second molars on both sides and a mandibular second molar on right side in the same patient which was observed during routine endodontic procedure. Three second permanent molars had single root, single canal and an accurate assessment of this unusual morphology was made with the help of radiographs and cone beam computed tomography (CBCT). This report extends the range of known possible anatomical variations to include teeth with lesser number of roots and canals.

Clinical evaluation of 3 Mix and Other Mix in non-instrumental endodontic treatment of necrosed primary teeth.

PROBLEM CONSIDERED: The objective was to evaluate and compare the clinical and radiographic efficacy of two different combinations of antibacterial drugs when used in non-instrumental endodontic treatment of necrosed primary teeth. MATERIALS AND METHOD: Forty teeth were randomly divided into two groups, viz. groups A and B having 20 teeth each. In Group A, 3 Mix (ciprofloxacin, metronidazole, and minocycline) and in Group B, Other Mix (ciprofloxacin, ornidazole, and minocycline) mixed with propylene glycol were used. Medication cavities of Group-A and B were filled with 3 Mix and Other Mix respectively followed by restoration of teeth. Clinical evaluation was done at 3 months whereas, clinical and radiographic evaluation was done at 6 and 12 months, respectively. RESULTS: Both the groups showed 100% clinical success whereas, radiographic success rate was 81% with 3 Mix and 92% with Other Mix but, was not statistically significant. CONCLUSIONS: Both the antibacterial pastes can be used effectively in non instrumental endodontic treatment of necrosed primary teeth and require more clinical trials to prove its efficacy.

Ectrodactyly-ectodermal dysplasia clefting syndrome (EEC syndrome).

Ectrodactyly-ectodermal dysplasia- clefting syndrome (also k/a. split hand- split foot malformation /split hand-split foot ectodermal dysplasia- cleft syndrome/ectodermal dysplasia cleft lip/cleft palate syndrome) a rare form of ectodermal dysplasia, is an autosomal dominant disorder inherited as a genetic trait and characterized by a triad of (i) ectrodactyly, (ii) ectodermal dysplasia and, (iii) & facial clefts.

Comparative evaluation of antimicrobial substantivity of different concentrations of chlorhexidine as a root canal irrigant: An in vitro study.

INTRODUCTION: The aim of this in vitro study was to evaluate and compare the antimicrobial substantivity of different concentrations of chlorohexidine as root canal irrigant by microbiological assay using mitis salivarius bacitracin streptomycin, MSBS agar plate. METHODS: Extracted single rooted permanent human teeth were selected as samples and randomly divided into four groups (three experimental, one control). The samples of the three experimental groups used different concentration of chlorhexidine, CHX (0.1%, 1% and 2%), deionized water used as an irrigant served as control. In each group the apices of teeth were sealed with composite and mounted on plaster blocks. Root canals were prepared using step back technique and enlarged upto no 80. With each change in the file size the corresponding irrigant was used and final irrigation was done with deionized water. Samples were taken with paper points at 12 h, 1 day, 2 days and 3 days respectively and stored in sterile phials which were then arranged on MSBS agar plates for microbiological assay. RESULTS AND CONCLUSION: Results were analysed by ANOVA and Tukey's HSD test showed that antibacterial substantivity of 2% CHX was best followed by 1% CHX and 0.1% CHX in decreasing order respectively.

A SEM evaluation of dentinal adaptation of root canal obturation with GuttaFlow and conventional obturating material.

OBJECTIVE: The aim of this in-vitro study was to compare dentinal adaptation of conventional obturating material and GuttaFlow in young permanent teeth of child patients. MATERIALS AND METHODS: Ten young permanent anterior teeth with closed foramen were selected for the study. Root canal preparations were carried out using a step back technique. The canal was alternately irrigated with 5.25% sodium hypochlorite solution and 17% EDTA and normal saline. After root canal preparation specimens were randomly divided into two groups (n=5). GROUP "A": Obturated with zinc-oxide eugenol sealer and gutta-percha cone using cold lateral obturation technique. GROUP "B": Obturated with GuttaFlow as per the manufacturer's instruction. The teeth were split into two halves. Five randomly chosen longitudinal split teeth samples each from Group "A" and Group "B" was observed under scanning electron microscope to access the interface between obturating material and dentin at the level of 2 mm from the apex. RESULTS: On statistical analysis the dentinal adaptation (µm) of Group A was observed with a mean of 0.52 and standard deviation 0.15, while the value of mean and standard deviation were noted 0.12 and 0.02 for Group B. The "t" value on comparison of Group A and Group B is 5.79 with "P " value of <0.001, which is highly significant. CONCLUSION: From result of the study it can be concluded Group B samples showed significantly superior seal when compared with Group A. Complete seal was not observed in any of Group A samples.

Regional Odontodysplasia Crossing Midline: A Rare Case Report.

Regional odontodysplasia is a nonhereditary, uncommon developmental abnormality of teeth. Females have more predilections for regional odontodysplasia. The enamel, dentin and pulp of teeth are affected and radiographically, teeth are described as "ghost teeth". Many of these :eeth do not erupt and have an increased risk for caries and periapical inflammation. Since the literature on regional odontodysplasia is limited, here is need to discuss this anomaly to have a better approach for the diagnosis and treatment.