RNAseq, transcriptome analysis and identification of DEGs involved in development and ripening of Fragaria chiloensis fruit.

Fragaria chiloensis (Chilean strawberry) is a native species that produces fruit with an exotic pinkish color and a fruity aroma. It has a non-climacteric pattern of fruit ripening, and it is the mother of the commercial Fragaria x ananassa. The ripening of F. chiloensis fruit seems stimulated by ABA, and a complete set of genes participate in its softening, color, and aroma development. In addition, a set of transcription factors regulate the entire process, but few of them have been described. Over the last two decades, RNA-seq was used to identify genes at three fruit development/ripening stages, named C2 (unripe, large green) to C4 (full ripe), in whole fruit and fruit without achenes. A total of 204,754 contigs were assembled considering all samples, obtaining an N50 of 1.125 bp. Differentially expressed genes (DEGs) between two samples were identified, obtaining a total of 77,181 DEGs. Transcripts for genes involved in ABA biosynthesis present high and differential expression during the C2, C3, and C4 stages. Besides, contigs corresponding to ABA receptors, which interact with a regulatory network, are also differentially expressed. Genes associated with cell wall remodeling and those involved in flavonoid synthesis were also differentially expressed. An interaction network was built considering differentially expressed genes for the phenylpropanoid and flavonoid molecular pathways and having FcMYB1 as a transcription factor regulator. Identifying key genes could give an option to control the ripening of this non-climacteric fruit.

Cierre de diastemas múltiples mediante el uso de resina universal Omnichroma. Reporte de caso clínico / Multiple diastema closure using Omnichroma universal resin. A case report

La presencia de los diastemas es una de las quejas estéticas más comunes entre los pacientes. La utilización de resinascompuestas es una buena opción estética y funcional para realizar restauraciones e implementar técnicasrestauradoras de forma mínimamente invasiva. La técnica de estratificación con resinas se introdujo en 1980 dando muy buenosresultadosenlaseleccióndecolor,perosiendoelprocedimientomáscomplejoyrequiriendograndeshabilidadestécnicasademás de un aumento de tiempo clínico. Los materiales de composite y técnicas restauradoras que ayudan al uso simplificado deprotocolos clínicos son muy procurados por los odontólogos para reducir el tiempo de trabajo en el sillón yminimizar la sensibilidad postoperatoria. Es porestos motivos,ademásde simplificar la toma de color,queTokuyamaDentaldesarrollólaresinaOmnichromaquedicetenerlahabilidaddecopiarelcolordelsustratodel diente remanente poco después de la fotopolimerización conocido como "Efecto Camaleón" utilizando un solo tono. El objetivode este artículo es el de describir detalladamente mediante un reporte de caso clínico el cierre de diastemas con laresinauniversal Omnichroma deTokuyama Dental.

The presence of diastemas is one of the most common aesthetic complaints among patients. The use of composite resins is a good aesthetic and functional option to perform restorations and implement restorative techniques in a minimally invasive way. The resin layering technique was introduced in 1980, giving very good results in color selection, but being the most complex procedure and requiring great technical skills in addition to an increase in clinical time. Composite materials and restorative techniquesthat aid in the simplified use of clinical protocols are highly sought after by dentists to reduce chair time and minimize post-operative sensitivity. It is for these reasons, in addition to simplifying shade taking, that Tokuyama Dental developed Omnichroma resin which claims to have the ability to copy the color of the remaining tooth substrate shortly after light curing known as the "Chameleon Effect" using a single shade. The objective of this article is to describe in detail, through a clinical case report, the closure of diastemas with Tokuyama Dental's Omnichroma universal resin.

Mass Education and Women's Autonomy: Evidence From Latin America.

Most low- and middle-income countries have implemented mass education reforms in the last few decades. Demographers and policymakers have posited that mass schooling would enhance women's autonomy and, therefore, accelerate population transformations in the Global South. However, gains in women's schooling may have unexpected implications for female autonomy in contexts where hypergamy norms-the ideal that men should marry down and women should marry up in education and other markers of status-are still dominant. This study addresses difficulties in evaluating the causal impact of additional education on women's autonomy by leveraging the timing of compulsory schooling reforms in three Latin American countries: Bolivia, Colombia, and Peru. Using Demographic and Health Surveys, I implement an instrumental variable design using random exposure to compulsory schooling laws as an instrument for years of education. Results show that for women who entered the school system as a result of compulsory reforms, further schooling decreased their level of autonomy in all countries-especially among women from rural Bolivia and Peru. Additional analyses suggest these results are explained by changes in the selection into schooling and the formation of unions defying hypergamy norms. Together, these findings highlight the importance of examining the returns to mass schooling considering population heterogeneity and the contextual meaning of women's education.

Fearing Such a Lady: University Expansion, Underemployment, and the Hypergamy Ideal in Kampala, Uganda.

In Uganda, the cultural norm of hypergamy, which dictates that husbands should have higher economic and social status than wives, is pervasive and influential. Yet hypergamy has recently been challenged by women's gains in education relative to men and by an unemployment crisis leaving educated young men unable to find steady work. Using interviews with recent university graduates in Kampala, we investigate how highly-educated young adults navigate frictions between the hypergamy ideal and these recent transformations in gendered status. Some women reduce the salience of hypergamy by preventing their relationships from becoming serious, while other women intentionally perform the role of submissive housewife while preserving their autonomy. Men reframe their romantic circumstances to underplay their inability to achieve economic hypergamy, portraying educated women as undesirable and characterizing their partners as non-materialistic. These findings reveal how demographic and economic changes reconfigure relationship norms, gendered power dynamics, and family formation processes.

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy.

The molecular mechanisms underlying inclination responses in trees are unclear. In this study, we identified a MADS-box transcription factor differentially expressed early after inclination in the stems of Pinus radiata D. Don. PrMADS10 has a CDS of 582 bp and encodes a group II MADS-box transcription factor. We measured highest accumulation of this transcript on the lower side of inclined pine stems. In an effort to identify putative targets, we stably transformed Arabidopsis thaliana with a 35S::PrMADS10 construct. Transcriptome analysis revealed 1,219 genes differentially-expressed, with 690 and 529 genes up- and down-regulated respectively, when comparing the transgenic and wild-type. Differentially-expressed genes belong to different biological processes, but were enriched in cell wall remodeling and phenylpropanoid metabolic functions. Interestingly, lignin content was 30% higher in transgenic as compared to wild-type plants consistent with observed changes in gene expression. Differentially expressed transcription factors and phenylpropanoid genes were analyzed using STRING. Several MYB and NAC transcription factors showed interactions with genes of the phenylpropanoid pathway. Together, these results implicate PrMADS10 as a regulatory factor, triggering the expression of other transcription factors and genes involved in the synthesis of lignin.

Short-run effects of accountability pressures on teacher policies and practices in the voucher system in Santiago, Chile.

This research analyzes the impact of the Adjusted Voucher Laws school rankings on low-performing schools in Santiago, Chile, and provides evidence on the effects of the pressures of accountability systems on teacher policies and practices. The empirical strategy is based on the fact that schools are ranked according to their position on a set of thresholds. We used a generalization of the traditional regression discontinuity design for the case where treatment assignment is determined by n variables. To gather information on teacher policies and practices, we conducted a survey of 4th-grade teachers in the Greater Santiago area. The results indicate that low-performing schools responded to the treatment by implementing policies that seek to improve their results in the short term. We also found no significant effects on teaching practices, suggesting that many of these changes are implemented top down from the school administrators, without involving teachers in the process.

The use of multidrug approach to uncover new players of the endomembrane system trafficking machinery.

Chemical Biology is a strong tool to perform experimental procedures to study the Endomembrane System (ES) in plant biology. In the last few years, several bioactive compounds and their effects upon protein trafficking as well as organelle distribution, identity, and size in plants and yeast have been characterized. Today, several of these chemical tools are widely used to perform mutant screens and establish the trafficking pathway of a given cellular component. This chapter is a guideline to perform multidrug approaches to study the endomembrane system in plant cells. This type of approach is a powerful and useful strategy to thoroughly determine the trafficking of a specific protein as well as to perform mutant screens based on phenotypes produced by drug treatments. On the other hand, a multidrug approach can address the characterization of a new bioactive molecule and find its cellular pathway target. Overall, this approach can unravel mechanisms and identify new players in endomembrane trafficking.

The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana.

Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 microM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism.

The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana

Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism.