[The development of a test system for determining antibodies to Bacteroides melaninogenicus using solid-phase immunoenzyme analysis]. / Razrabotka test-sistemy dlia opredeleniia antitel k Bacteroides melaninogenicus s pomoshch'iu tverdofaznogo immunofermentnogo analiza.

A test system for enzyme immunoassay of antibodies to Bacteroides melaninogenicus has been developed. The authors describe a method for the preparation of the protein and polysaccharide antigens with the use of ultrasonic, lysozyme, and beta-naphthol treatment. A total of 105 blood serum samples, 46 samples of gingival blood and gingival fluid from patients with maxillofacial phlegmons and periodontitis were examined, making use of the 1 +/- 3 omega index (from the values in healthy donors). The results evidence statistically significant difference in antibody levels of patients from whom B. melaninogenicus were isolated and of those with the negative results of bacteriologic analysis. The titers of antibodies to B. melaninogenicus in the blood and gingival fluid of patients with periodontitis were in good correlation.

[Etiology of inflammatory maxillofacial diseases and estimation of the effectiveness of antibacterial drugs in vitro]. / Etiologiia vospalitel'nykh zabolevanii cheliustno-litsevoi oblasti i otsenka éffektivnosti antibakterial'nykh preparatov in vitro.

The results of identification of 710 clinical strains of anaerobic microorganisms isolated from the pathological foci of patients with maxillofacial diseases are presented. The species composition of the microflora associations in the cases with abscesses, phlegmon, lymphadenitis, osteomyelitis and parodontitis is described. Along with a high frequency of nonsporulating anaerobes, staphylococci, microaerophilic streptococci and in the cases with parodontitis actinomycetes, Bacillus licheniformis and Bacillus coagulans strains (1.6-15% of the isolated strains) were first detected in cases with various forms of the disease. Two groups of the drugs effective against the anaerobes were identified by the data on the antibiotic sensitivity. The lowest MICs along with the activity broad spectrum were defined for gramicidin, levomycetin and nitazol.

[The effect of nitazole preparations on the microflora in periodontitis]. / Vliianie preparatov nitazola na mikrofloru pri parodontite.

Twenty-three patients with generalized periodontitis were examined. Microbiologic examination of the periodontal pouch contents, making use of anaerobic cultivation, resulted in isolation and identification of 103 strains, 76 (72.8%) of which were referred to obligate anaerobes. Facultative anaerobes made up 27.2% of the total number of the isolates. Therapy with water-soluble nitasole resulted in reduction of the number of microorganisms, decrease or arrest of pus discharge from the pouch, resolution of the edema and gingival ridge hyperemia, etc. Diffusion of nitasole (water-soluble) and cliostom from various dressings used in periodontitis was studied and the efficacy of combining these dressings revealed.

[Effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections]. / Deistvie na vozbuditelei odontogennoi infektsii antibiotikov i antibakterial'nykh komponentov, vkhodiashchikh v sostav preparatov dlia mestnogo lecheniia gnoinykh ran.

The results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. In the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to Bacteroides, Fusobacterium, Peptococcus, Peptostreptococcus, Veillonella and Actinomyces. Significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. There was observed high sensitivity of the obligate anaerobes to gramicidin (0.02 micrograms/ml), nitazol (10 micrograms/ml), levomycetin and tetracycline (60 micrograms/ml). Antiseptics such as dioxidine and chlorhexidine used locally showed satisfactory results. The above mentioned drugs and especially levomycetin were also rather active against facultative organisms in associations of pathogens causing odontogenic infections: Bacillus coagulans, B. licheniformis, Pseudomonas sp., Acinetobacter calcoaceticus, Staphylococcus sp. and Streptococcus sp.

[Absence of the effect of magnetic field on dissolving oxygen in aqueous solutions]. / Ob otsutstvii vliianiia magnitnogo polia na rastvorenie kisloroda v vodnykh rastvorakh.

To check a number of experimental works the effect of constant magnetic field with induction 0.35 T on dissolving of oxygen in water and in 0.1 n of NaCl was studied. The magnetic field of the indicated value was shown not to affect solubility and the rate of dissolving of oxygen in water and in aqueous solution of electrolyte. These findings well agree with theoretical concepts and make it impossible to consider direct effect of the magnetic field on the molecules of paramagnetic oxygen as one of possible mechanisms of the magnetic field effect on biological systems.

[Composition and structure of phospholipids in hydrocarbon yeasts. I. Phosphatidylcholine and phosphatidylethanolamine]. / Sostav i struktura fosfolipidov uglevodorodnykh drozhzhei. I. Fosfatidilkholin i fosfatidilétanolamin.

From fooder yeast grown on purified liquid n-parafins two fractions--phosphatydylcholin and phosphatydyletanolamin--have been isolated. Structure, composition and properties of these compounds have been studied. It is established that double bonds in fatty acids ends are located near their polar group.