ABSTRACT
The Latvian local goat (LVK) breed represents the only native domestic goat breed in Latvia, but its limited population places it within the endangered category. However, the LVK breed has not yet undergone a comprehensive genetic characterization. Therefore, we completed whole genome sequencing to reveal the genetic foundation of the LVK breed while identifying genetic traits linked to the somatic cell count (SCC) levels. The study included 40 genomes of LVK goats sequenced to acquire at least 35x or 10x coverage. A Principal component analysis, a genetic distance tree, and an admixture analysis showed LVK's similarity to some European breeds, such as Finnish Landrace, Alpine, and Saanen, which aligns with the breed's history. An analysis of genome-wide heterozygosity, nucleotide diversity, and LD analysis indicated that the LVK population exhibits substantial levels of genetic diversity. LVK genome was dominated by short runs of homozygosity (ROHs, ≤ 500 kb) with a median length of 25 kb. With FROH 2.49%, average inbreeding levels were low; however, FROH ranged broadly from 0.13 to 12.2%. With the exception of one pure-blood breeding buck exhibiting FROH of 9.3% and FSNP of 8.5%, animals with at least 66% LVK ancestry showed moderate or no inbreeding. Overall, this study demonstrated that the LVK goats can be differentiated from imported breeds, although the population has a complex genetic structure. We were able to identify potential genetic traits associated with SCC levels, although the kinship of the animals and the heterogenic substructure of the population might have largely influenced the association analysis. We identified 26 genetic variants associated with SCC levels, which included the potentially relevant SNP rs662053371 in the OSBPL8 gene, indicating a potential signal linked to lipid metabolism in goats. To conclude, these findings present valuable insight into the genetic structure of the LVK breed for the conservation of local genetic resources.
Subject(s)
Genetic Variation , Goats , Animals , Goats/genetics , Latvia , Breeding , Cell Count/veterinary , Polymorphism, Single Nucleotide , Whole Genome Sequencing/veterinary , Female , Male , GenomeABSTRACT
UNLABELLED: Low-penetrance gene variants and their combinations are topical study objects in breast cancer pathogenesis. Single nucleotide polymorphism rs61764370, localized in 3Õ UTR of KRAS gene, plays an important role in the development and progression of several cancers. The aim of our study was to determine the KRAS variant impact on breast cancer morbidity. PATIENTS AND METHODS: 2214 patients diagnosed with breast cancer and 861 healthy controls were screened for KRAS variant by RFLP method. Available clinical data were collected and processed using statistical analysis methods. Results of present study suggest the KRAS variant impact on breast cancer development risk in premenopausal women, but it has no effect on breast cancer prognosis. We did not observe any KRAS variant effect on breast cancer patient 10-year disease-specific survival rates.
Subject(s)
Breast Neoplasms/genetics , Polymorphism, Single Nucleotide/genetics , Proto-Oncogene Proteins p21(ras)/genetics , Adult , Aged , Aged, 80 and over , Case-Control Studies , Female , Humans , Middle Aged , PrognosisABSTRACT
Consideration of ethnic features of adaptation of students to educational activity in high school is needed as to optimize the learning process and to preserve the functional reserves of the organism. We investigated the characteristics of the psychological status and correlated them with the heart rhythm variability in students Yakuts with varying different effectiveness of educational success. Regression analysis of the relationship of personality psychological characteristics of students with indicators of the spectral power of heart rate showed that more adaptive resource autonomic regulation of the heart have people with an average level of introversion and of personal anxiety indicators corresponding to the transition from moderate to high degree and also in individuals with an average levels of neuroticism. Revealed higher effectiveness of examinations students introverted compared to extroverts and students with high personal anxiety relatively to persons with moderate personal anxiety. High efficiency of intellectual activity of students is achieved through high tension mechanisms vegetative regulation of the heart.
