The interplay of homology-directed repair pathways in the repair of zebularine-induced DNA-protein crosslinks in Arabidopsis.

DNA-protein crosslinks (DPCs) are highly toxic DNA lesions represented by proteins covalently bound to the DNA. Persisting DPCs interfere with fundamental genetic processes such as DNA replication and transcription. Cytidine analog zebularine (ZEB) has been shown to crosslink DNA METHYLTRANSFERASE1 (MET1). Recently, we uncovered a critical role of the SMC5/6-mediated SUMOylation in the repair of DPCs. In an ongoing genetic screen, we identified two additional candidates, HYPERSENSITIVE TO ZEBULARINE 2 and 3, that were mapped to REGULATOR OF TELOMERE ELONGATION 1 (RTEL1) and polymerase TEBICHI (TEB), respectively. By monitoring the growth of hze2 and hze3 plants in response to zebularine, we show the importance of homologous recombination (HR) factor RTEL1 and microhomology-mediated end-joining (MMEJ) polymerase TEB in the repair of MET1-DPCs. Moreover, genetic interaction and sensitivity assays showed the interdependency of SMC5/6 complex, HR, and MMEJ in the homology-directed repair of MET1-DPCs in Arabidopsis. Altogether, we provide evidence that MET1-DPC repair in plants is more complex than originally expected.

NSE5 subunit interacts with distant regions of the SMC arms in the Physcomitrium patens SMC5/6 complex.

Structural maintenance of chromosome (SMC) complexes play roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of SMC proteins with a unique structure consisting of an ATPase head, long arm, and hinge. SMC complexes form long rod-like structures, which can change to ring-like and elbow-bent conformations upon binding ATP, DNA, and other regulatory factors. These SMC dynamic conformational changes are involved in their loading, translocation, and DNA loop extrusion. Here, we examined the binding and role of the PpNSE5 regulatory factor of Physcomitrium patens PpSMC5/6 complex. We found that the PpNSE5 C-terminal half (aa230-505) is required for binding to its PpNSE6 partner, while the N-terminal half (aa1-230) binds PpSMC subunits. Specifically, the first 71 amino acids of PpNSE5 were required for binding to PpSMC6. Interestingly, the PpNSE5 binding required the PpSMC6 head-proximal joint region and PpSMC5 hinge-proximal arm, suggesting a long distance between binding sites on PpSMC5 and PpSMC6 arms. Therefore, we hypothesize that PpNSE5 either links two antiparallel SMC5/6 complexes or binds one SMC5/6 in elbow-bent conformation, the later model being consistent with the role of NSE5/NSE6 dimer as SMC5/6 loading factor to DNA lesions. In addition, we generated the P. patens Ppnse5KO1 mutant line with an N-terminally truncated version of PpNSE5, which exhibited DNA repair defects while keeping a normal number of rDNA repeats. As the first 71 amino acids of PpNSE5 are required for PpSMC6 binding, our results suggest the role of PpNSE5-PpSMC6 interaction in SMC5/6 loading to DNA lesions.

Loneliness and Mental Health in Response to Early and Late COVID-19 Outbreak: A Cross-Sectional Study of Czech Adults Aged 50 and Over.

The unpredictable spread of the COVID-19 pandemic, national lockdowns, and public health measures implemented in the Czech Republic had a negative effect on older adults' mental health and loneliness. The 2,631 older adults in 2020 and 2,083 older adults in 2021 used in this study formed a nationally representative sample of the Survey of Health, Ageing and Retirement in Europe (SHARE). Almost every third older adult experienced feelings of loneliness in both stages of the COVID-19 outbreak. Loneliness increased in 2021 in those individuals who reported their physical health as poor, who felt nervous, sad or depressed, and who had ever left their home since the outbreak. According to age-related drivers of loneliness, feelings of loneliness were prevalent (40% vs. 45%) among younger retirees in both waves. In both data sets, declared feelings of sadness or depression were the strongest sustainable predictor of loneliness (2020 and 2021 models, OR = 3.69; 95% CI [2.90, 4.69] and OR = 2.55; [1.97, 3.30]). Being a woman and feeling nervous equated with a higher likelihood of feeling lonely compared to counterparts. Policy makers should therefore aim to carefully improve psychosocial and health-related consequences faced by this vulnerable population during the pandemic and beyond.

The relations between Czech undergraduates' motivation and emotion in self-regulated learning, learning engagement, and academic success in blended course designs: Consistency between theory-driven and data-driven approaches.

Combining theory-driven and data-driven approaches, this study used both self-reported and observational measures to examine: (1) the joint contributions of students' self-reported undergraduates' motivation and emotion in their self-regulated learning, their observed online learning interactions, and their academic success in blended course designs; and (2) the extent to which the self-reported and observational measures were consistent with each other. The participants in the study were 54 social sciences undergraduates in the Czech Republic. The participants' self-reported self-efficacy, intrinsic goals, and anxiety were assessed using a Czech version of three scales from the Motivated Strategies for Learning Questionnaire. Their online engagement was represented by students' observed frequency of interactions with the six online learning activities recorded in the learning management system. The results of a hierarchical regression analysis showed that the self-reported and observational measures together could explain 71% of variance in academic success, significantly improving explanatory power over using self-reported measures alone. Departing from the theory-driven approach, students were clustered as better and poorer self-regulated learners by their self-reports, and one-way ANOVAs showed that better self-regulated learners had significantly more frequent online interactions with four out of six online learning activities and better final exam results. Departing from the data-driven approach, students were clustered as higher and lower online-engaged learners by the observed frequency of their interaction with online learning activities. One-way ANOVAs showed that higher online-engaged learners also reported having higher self-efficacy and lower anxiety. Furthermore, the strong association between the students' profiles in both self-reported measures and observational measures in cross-tabulation analyses showed that the majority of better self-regulated learners by self-reporting also had higher online engagement by observation, whereas the majority of poorer self-regulated learners by self-reporting were lower online-engaged learners, demonstrating consistency between theory-driven and data-driven approaches.

Factor structure of the Self-Regulation Questionnaire among adult learners from Poland, Serbia, Slovakia, and the Czech Republic.

BACKGROUND: One of the most significant human qualities is the ability to develop, implement, and flexibly maintain planned behaviour in order to achieve one's goals. Self-regulation has become a relatively well-researched area in the field of psychology and pedagogy. However, empirically valid and reliable instrument is still missing across European context. The primary goal of this research was to analyze the psychometric properties of the Czech version of the Self-Regulation Questionnaire (SRQ-CZ) among adult learners from Poland, Serbia, Slovakia, and the Czech Republic. OBJECTIVE: The aim of the present study was to examine the factor structure and psychometric properties of the SRQ-CZ validated in the Czech educational context in a multi-cultural sample. METHODS: A total of 1711 adult learners from European countries including Poland, Serbia, Slovakia, and the Czech Republic completed the SRQ-CZ. The first step to reviewing the validity of the SRQ-CZ included testing face validity. Furthermore, exploratory factor analysis (EFA) was performed on half the sample and confirmatory factor analysis (CFA) on the other half. Measurement invariance was conducted across gender, age, and country followed by the evaluation of the reliability of the final instrument. RESULTS: EFA showed that a three-factor structure best fit the data. The originally proposed Impulse Control and Self-Direction are merged into one distinct factor, while Decision Making and Goal Orientation comprise the other two. Goodness-of-fit statistics yielded from CFA showed a good fit for the model, introducing a reliable and measurement invariant instrument. CONCLUSION: The present study used a diverse multi-cultural sample to explore the factorial structure and psychometric properties of the SRQ-CZ. A three-factor model was obtained as the result of the exploratory and confirmatory factor analyses. Further analysis aiming at measurement invariance, comparing the sample according to gender, age, and country, led to satisfactory results. The only exception was a lack of model fit in the case of Serbia. Although further psychometric evaluation of the SRQ-CZ is still needed, the presented results constitute significant findings, confirming instrument validity and utility as a measure of generalized self-regulation capacity across adult learners in European educational context.

Risk Factors Affecting Mental Health During The Early Stages Of The Covid-19 Pandemic In High-Risk 50+ Population In The Czech Republic.

This study deals with key factors affecting mental health during the COVID-19 pandemic. The results are based on the longitudinal representative 50+ population-based multi-country panel study Survey of Health, Aging and Retirement in Europe (SHARE) in which representative samples of individuals in European countries plus Israel were collected. The goal is to turn the challenges of aging into opportunities as well as provide valid and reliable data for evidence-based policies. A total of 2,631 participants were interviewed by telephone in the Czech Republic during the initial stages of the COVID-19 outbreak in 2020. After a descriptive analysis showed that participants complied well with most preventative and protective COVID-19 measures, subsequent modeling using logistic regression analysis and a decision tree algorithm identified key determinants that have contributed to an understanding of variation in declared feelings of depression. We found that nervousness, previously unexperienced trouble with sleep, and self-rated health status before the COVID-19 outbreak and gender remained significant predictors of depression, from which statistically significant different categories concerning the percentage of depression could be formed. These identified potential risk factors should be considered in planning further supportive strategies for high-risk 50+ population.

Coping Strategies and Academic Motivation: The Mediating Effect of Achievement Emotions

The current study seeks to determine whether different achievement emotions (positive and negative) mediate the relationship between coping strategies (proactive and preventive coping) and academic motivation in a representative sample of the Czech adult population (N= 1,025). A cross-sectional research design featuring path analysis was used to explore the mediated effects of theoretical models. The results revealed that positive achievement emotions (i.e. enjoyment, hope, and pride) work well as moderators between positively future-oriented coping behavior and motivation to study. However, model fit, including mediating role of negative achievement emotions (i.e. anger, anxiety, shame, hopelessness, and boredom) as moderators, was very poor. Moreover, the measurement invariance of the data-fitting model of positive emotions indicated that the same construct was being measured across gender and age groups. Finally, implications of the findings regarding mental health promotion, limitations of the study, and future research are discussed (AU)

Mediation pattern of proactive coping and social support on well-being and depression

Proactive coping is a multidimensional and future-looking quality of life strategy that can predict positive outcomes and regulate distress. Recently, social support has been seen as an essential resource for effective coping with stressors. On this basis, a cross-sectional study examining a theoretical model was investigated using a path analysis. It was hypothesized that social support would be associated with proactive coping in the synergistic relationship and in relation to the positive psychological variable of well-being. Moreover, direct relationships between well-being and feelings of depression were expected. In a sample of 482 full-time university students attending public university, the results showed that social support and comparable proactive coping directly contributed to an increase in well-being. Furthermore, well-being was directly related to depression. Besides direct effects, an indirect pathway from social support to well-being was tested confirming the hypothesis that proactive coping functions as a partial mediator between social support and well-being. Generalizability of the findings was tested across gender and age performing multi-group analyses. Furthermore, practical implications, study limitations, and future research are discussed

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Testing the Uni-dimensionality of Proactive and Preventive Copingpp

Proactive coping and preventive coping are oriented on certain future rewards and challenges that are not appraised as threats. The objective of the study is to answer the question whether the coping scales are both uni-dimensional constructs as measured by the Proactive Coping Inventory. The underlying factor structure was empirically assessed using exploratory factor analysis in university students enrolled in a traditional face-to-face course delivery format in a medium-sized public university in the Czech Republic. Moreover, item analysis and correlations with other personality variables were carried out. The present study opens discussion about construct validity that has not been tested in the presented educational environment (AU)

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