Individual variation in rod absorbance spectra correlated with opsin gene polymorphism in sand goby (Pomatoschistus minutus).

Rod absorbance spectra, characterized by the wavelength of peak absorbance (lambda(max)) were related to the rod opsin sequences of individual sand gobies (Pomatoschistus minutus) from four allopatric populations [Adriatic Sea (A), English Channel (E), Swedish West Coast (S) and Baltic Sea (B)]. Rod lambda(max) differed between populations in a manner correlated with differences in the spectral light transmission of the respective water bodies [lambda(max): (A) approximately 503 nm; (E and S) approximately 505-506 nm; (B) approximately 508 nm]. A distinguishing feature of B was the wide within-population variation of lambda(max) (505.6-511.3 nm). The rod opsin gene was sequenced in marked individuals whose rod absorbance spectra had been accurately measured. Substitutions were identified using EMBL/GenBank X62405 English sand goby sequence as reference and interpreted using two related rod pigments, the spectrally similar one of the Adriatic P. marmoratus (lambda(max) approximately 507 nm) and the relatively red-shifted Baltic P. microps (lambda(max) approximately 515 nm) as outgroups. The opsin sequence of all E individuals was identical to that of the reference, whereas the S and B fish all had the substitution N151N/T or N151T. The B fish showed systematic within-population polymorphism, the sequence of individuals with lambda(max) at 505.6-507.5 nm were identical to S, but those with lambda(max) at 509-511.3 nm additionally had F261F/Y. The substitution F261Y is known to red-shift the rod pigment and was found in all P. microps. We propose that ambiguous selection pressures in the Baltic Sea and/or gene flow from the North Sea preserves polymorphism and is phenotypically evident as a wide variation in lambda(max).

Polymorphism of the rod visual pigment between allopatric populations of the sand goby (Pomatoschistus minutus): a microspectrophotometric study.

Absorbance spectra were measured by microspectrophotometry in retinal rods of sand gobies (Pomatoschistus minutus) from four allopatric populations (Baltic Sea, Swedish west coast, English Channel and Adriatic Sea). Mean (+/- S.E.M.) wavelengths of maximum absorbance (lambda(max)) were 508.3+/-0.5 nm, 505.4+/-0.2 nm, 506.2+/-0.3 nm and 503.0+/-0.3 nm, respectively. Pairwise comparison between the populations (post-ANOVA Scheffe's test) shows that each of the lambda(max) differences, except that between the Swedish west coast and the English Channel, is statistically significant (P<0.05). The shapes of the absorbance spectra indicated that the pigments were A1 rhodopsins with no measurable admixture of the A2 chromophore. Thus, the differences indicate polymorphism in the protein part (opsin) of the pigment. Convolution of A1 templates for lambda(max) values 508.3 nm and 503.0 nm with quantum spectra of the downwelling light at two locations at the south-west coast of Finland indicated that a 13-19% improvement in quantum catch would accrue in the Baltic environment from the 5.3 nm red-shift of the rod pigment of Baltic compared with Adriatic sand gobies.