Imaging elastic waves in solids: how to use laser feedback interferometry to visualize them.

The use of ultrasonic elastic waves is a well established technique for non-destructive testing of materials and structures, in particular to exploit the interaction of waves with structural features to detect and characterize defects. Optical methods offer the advantage of visualising the distribution of elastic waves in a non-contact manner without disturbing the elastic wave. In this work we propose a laser feedback interferometry (LFI) based system as a cost effective, non-contact, alternative to a well established laser Doppler vibrometer technique. We demonstrate the visualization of the elastic waves, using an example of an elastic wave propagating through a prismatic acrylic rod. We show that the ultra-compact and simple implementation of LFI enables accurate visualization of the elastic waves in solids, and opens the pathway to a range of new opportunities in ultrasonic non-destructive testing and evaluation.

A systematic review of ultrasound-mediated drug delivery to the eye and critical insights to facilitate a timely path to the clinic.

Ultrasound has long been identified as a promising, non-invasive modality for improving ocular drug delivery across a range of indications. Yet, with 20 years of learnings behind us, clinical translation remains limited. To help address this, and in accordance with PRISMA guidelines, the various mechanisms of ultrasound-mediated ocular drug delivery have been appraised, ranging from first principles to emergent applications spanning both ex vivo and in vivo models. The heterogeneity of study methods precluded meta-analysis, however an extensive characterisation of the included studies allowed for semi-quantitative and qualitative assessments. Methods: In this review, we reflected on study quality of reporting, and risk of bias (RoB) using the latest Animal Research: Reporting of In Vivo Experiments (ARRIVE 2.0) guidelines, alongside the Systematic Review Centre for Laboratory animal Experimentation (SYRCLE) RoB tools. Literature studies from 2002 to 2022 were initially characterised according to methods of ultrasound application, ultrasound parameters applied, animal models employed, as well as safety and efficacy assessments. This exercise contributed to developing a comprehensive understanding of the current state of play within ultrasound-mediated ocular drug delivery. The results were then synthesised and processed into a guide to aid future study design, with the goal of improving the reliability of data, and to support efficient and timely translation to the clinic. Results: Key attributes identified as hindering translation included: poor reporting quality and high RoB, skewed use of animals unrepresentative of the human eye, and the over reliance of reductionist safety assessments. Ex vivo modelling studies were often unable to have comprehensive safety assessments performed on them, which are imperative to determining treatment safety, and represent a pre-requisite for clinical translation. Conclusion: With the use of our synthesised guide, and a thorough understanding of the underlying physicochemical interactions between ultrasound and ocular biology provided herein, this review offers a firm foundation on which future studies should ideally be built, such that ultrasound-mediated ocular drug delivery can be translated from concept to the coalface where it can provide immense clinical benefit.

Mechanical and Tribological Performances of Thermoplastic Polymers Reinforced with Glass Fibres at Variable Fibre Volume Fractions.

High wear rates and frictional coefficients have always been the primary reasons for limiting the service life of critical elements such as pumps, couplings, bushings, bearings and gears. The premature and erratic failures are costing the industries extensive amounts of money every year. Additionally, under severe service conditions, the wear resistance requirements are higher, which greatly hinders the application of neat thermoplastics in different sectors. Hence, it is vital to enhance the tribological characteristics of thermoplastics. The mechanical and tribological properties of Polyamide 6, Thermoplastic Polyurethane, and glass fibre reinforced (GFR) Polyadmide 6 Composites of variable fibre volume fractions were investigated. Pin specimens of Polyamide 6 reinforced with (25%, 33%, and 50%) by volume of fibres were fabricated by an injection moulding process. The specimens were tested for tensile, compression, hardness, and wear under dry abrasive conditions using a pin-on-disc setup. Furthermore, the samples were scanned using micro-computed tomography (micro-CT), and the worn-out samples were analysed using field emission scanning electron microscopy. The experimental results showed that the fibre volume fraction was inversely proportional to the wear resistance of the prepared composite materials. This research will enable the industry partners to supply cutting-edge technologies to the global oil and gas industry that not only minimizes the well running cost but also improves the well resilience.

Multi-physics study of acoustofluidic delivery agents' clustering behavior.

Acoustofluidicly manipulated microbubbles (MBs) and echogenic liposomes (ELIPs) have been suggested as drug delivery systems for the 'on demand' release of drug in target tissue. This requires a clear understanding of their behaviour during ultrasonication and after ultrasonication stops. The main focus of this study is to investigate the behaviour of MBs and ELIPs clusters after ultrasonication stops and the underlaying cause of cluster diffusion considering electrostatic repulsion, steric repulsion and Brownian motion. It also examines the capability of existing models used to predict MBs' attraction velocity due to secondary radiation force, on predicting ELIPs' attraction velocity. Tunable resistive pulse sensing (TRPS) and phase analysis light scattering (PALS) techniques were used to measure zeta potentials of the agents and the size distributions were measured using TRPS. The zeta potentials were found to be -2.43 mV and -0.62 mV for Definity™ MBs, and -3.62 mV and -2.35 mV for ELIPs using TRPS and PALS, respectively. Both agents were shown to have significant cluster formation at pressures as low as 6 kPa. Clusters of both agents were shown to diffuse as sonication stops at a rate that approximately equals the sum of the diffusion coefficients of the agents forming them. The de-clustering behaviours are due to Brownian motion as no sign of electrostatic repulsion was observed and particles movements were observed to be faster for smaller diameters. These findings are important to design and optimise effective drug delivery systems using acoustofluidically manipulated MBs and ELIPs.

Shell properties and concentration stability of acoustofluidic delivery agents.

This paper investigates the shell elastic properties and the number-concentration stability of a new acoustofluidic delivery agent liposome in comparison to Definity™, a monolayer ultrasonic contrast agent microbubble. The frequency dependent attenuation of an acoustic beam passing through a microbubble suspension was measured to estimate the shell parameters. The excitation voltage was adjusted to ensure constant acoustic pressure at all frequencies. The pressure was kept at the lowest possible magnitude to ensure that effects from nonlinear bubble behaviour which are not considered in the analytical model were minimal. The acoustofluidic delivery agent shell stiffness Sp and friction Sf parameters were determined as (Sp = 0.11 N/m, Sf = 0.31 × 10-6 Kg/s at 25 °C) in comparison to the Definity™ monolayer ultrasound contrast agent which were (Sp = 1.53 N/m, Sf = 1.51 × 10-6 Kg/s at 25 °C). When the temperature was raised to physiological levels, the friction coefficient Sf decreased by 28% for the monolayer microbubbles and by only 9% for the liposomes. The stiffness parameter Sp of the monolayer microbubble decreased by 23% while the stiffness parameter of the liposome increased by a similar margin (27%) when the temperature was raised to 37 °C. The size distribution of the bubbles was measured using Tunable Resistive Pulse Sensing (TRPS) for freshly prepared microbubbles and for bubble solutions at 6 h and 24 h after activation to investigate their number-concentration stability profile. The liposome maintained >80% of their number-concentration for 24 h at physiological temperature, while the monolayer microbubbles maintained only 27% of their number-concentration over the same period. These results are important input parameters for the design of effective acoustofluidic delivery systems using the new liposomes.

Evaluation of the mechanical compatibility of additively manufactured porous Ti-25Ta alloy for load-bearing implant applications.

Integrating porous networks in load-bearing implants is essential in order to improve mechanical compatibility with the host tissue. Additive manufacturing has enabled the optimisation of the mechanical properties of metallic biomaterials, notably with the use of novel periodic regular geometries as porous structures. In this work, we successfully produced solid and lattice structures made of Ti-25Ta alloy with selective laser melting (SLM) using a Schwartz primitive unit-cell for the first time. The manufacturability and repeatability of the process was assessed through macrostructural and microstructural observations along with compressive testing. The mechanical properties are found to be suitable for bone replacement applications, showing significantly reduced elastic moduli, ranging from 14 to 36 GPa depending on the level of porosity. Compared to the conventionally used biomedical Ti-6Al-4V alloy, the Ti-Ta alloy offers superior mechanical compatibility for the targeted applications with lower elastic modulus, similar strength and higher ductility, making the Ti-25Ta alloy a promising candidate for a new generation of load-bearing implants.

The hyperelastic and failure behaviors of skin in relation to the dynamic application of microscopic penetrators in a murine model.

In-depth understanding of skin elastic and rupture behavior is fundamental to enable next-generation biomedical devices to directly access areas rich in cells and biomolecules. However, the paucity of skin mechanical characterization and lack of established fracture models limits their rational design. We present an experimental and numerical study of skin mechanics during dynamic interaction with individual and arrays of micro-penetrators. Initially, micro-indentation of individual skin strata revealed hyperelastic moduli were dramatically rate-dependent, enabling extrapolation of stiffness properties at high velocity regimes (>1ms-1). A layered finite-element model satisfactorily predicted the penetration of micro-penetrators using characteristic fracture energies (∼10pJµm-2) significantly lower than previously reported (≫100pJµm-2). Interestingly, with our standard application conditions (∼2ms-1, 35gpistonmass), ∼95% of the application kinetic energy was transferred to the backing support rather than the skin ∼5% (murine ear model). At higher velocities (∼10ms-1) strain energy accumulated in the top skin layers, initiating fracture before stress waves transmitted deformation to the backing material, increasing energy transfer efficiency to 55%. Thus, the tools developed provide guidelines to rationally engineer skin penetrators to increase depth targeting consistency and payload delivery across patients whilst minimizing penetration energy to control skin inflammation, tolerability and acceptability. STATEMENT OF SIGNIFICANCE: The mechanics of skin penetration by dynamically-applied microscopic tips is investigated using a combined experimental-computational approach. A FE model of skin is parameterized using indentation tests and a ductile-failure implementation validated against penetration assays. The simulations shed light on skin elastic and fracture properties, and elucidate the interaction with microprojection arrays for vaccine delivery allowing rational design of next-generation devices.

Detection of light and vibration modulates bioluminescence intensity in the glowworm, Arachnocampa flava.

Glowworms are larval fungus gnats that emit light from a specialised abdominal light organ. The light attracts small arthropod prey to their web-like silk snares. Larvae glow throughout the night and can modulate their bioluminescence in response to sensory input. To better understand light output regulation and its ecological significance, we examined the larvae's reaction to light exposure, vibration and sound. Exposure to a 5-min light pulse in the laboratory causes larvae to exponentially decrease their light output over 5-10 min until they completely switch off. They gradually return to pre-exposure levels but do not show a rebound. Larvae are most sensitive to ultraviolet light, then blue, green and red. Vibration of the larval snares results in a several-fold increase in bioluminescence over 20-30 s, followed by an exponential return to pre-exposure levels over 15-30 min. Under some conditions, larvae can respond to vibration by initiating bioluminescence when they are not glowing; however, the response is reduced compared to when they are glowing. We propose that inhibitory and excitatory mechanisms combine to modulate bioluminescence intensity by regulating biochemical reactions or gating the access of air to the light organ.

Scattering characteristics of Lamb waves from debondings at structural features in composite laminates.

This article investigates the scattering characteristics of Lamb waves from a debonding at a structural feature in a composite laminate. This study specifically focuses on the use of the low frequency fundamental antisymmetric (A(0)) Lamb wave as the incident wave for debonding detection. Three-dimensional finite element (FE) simulations and experimental measurements are used to investigate the scattering phenomena. Good agreement is obtained between the FE simulations and experimental results. Detailed parameter studies are carried out to further investigate the relationship between the scattering amplitudes and debonding sizes. The results show that the amplitude of the scattered A(0) Lamb wave is sensitive to the debonding size, which indicates the potential of using the low frequency A(0) Lamb wave as the interrogating wave for debonding detection and monitoring. The findings of the study provide improved physical insights into the scattering phenomena, which are important to further advance damage detection techniques and optimize transducer networks.

Influence of stacking sequence on scattering characteristics of the fundamental anti-symmetric Lamb wave at through holes in composite laminates.

This paper investigates the scattering characteristics of the fundamental anti-symmetric (A(0)) Lamb wave at through holes in composite laminates. Three-dimensional (3D) finite element (FE) simulations and experimental measurements are used to study the physical phenomenon. Unidirectional, bidirectional, and quasi-isotropic composite laminates are considered in the study. The influence of different hole diameter to wavelength aspect ratios and different stacking sequences on wave scattering characteristics are investigated. The results show that amplitudes and directivity distribution of the scattered Lamb wave depend on these parameters. In the case of quasi-isotropic composite laminates, the scattering directivity patterns are dominated by the fiber orientation of the outer layers and are quite different for composite laminates with the same number of laminae but different stacking sequence. The study provides improved physical insight into the scattering phenomena at through holes in composite laminates, which is essential to develop, validate, and optimize guided wave damage detection and characterization techniques.

Scattering of the fundamental anti-symmetric Lamb wave at delaminations in composite laminates.

An analysis of the scattering characteristics of the fundamental anti-symmetric (A(0)) Lamb wave at a delamination in a quasi-isotropic composite laminate is presented. Analytical solutions for this problem do not exist due to the anisotropic nature and multilayer characteristics of composite laminates. This study uses a three-dimensional finite element (FE) method and experimental measurements to provide physical insight into the scattering phenomena. Good agreement is found between simulations and experimental measurements. The results show that the A(0) Lamb wave scattering at a delamination in composite laminates is much more complicated than the scattering at a defect in isotropic plates. Scatter amplitudes and scatter directivity distributions depend on the delamination size to wavelength ratio and the through-thickness location of the delamination damage. The study also investigates the feasibility of the common experimental practice of simulating delamination damage by bonding masses to the surface of composite laminates for guided wave damage detection and characterization methodologies verifications. The results suggest that care is required to use bonded masses to simulate delamination damage for verifying and optimizing damage characterization techniques. In summary, the results of the investigation help to further advance the use of the A(0) Lamb wave for damage detection and characterization.

Introducing the pressure-sensing palatograph--the next frontier in electropalatography.

PRIMARY OBJECTIVE: To extend the capabilities of current electropalatography (EPG) systems by developing a pressure-sensing EPG system. An initial trial of a prototype pressure-sensing palate will be presented. RESEARCH DESIGN: The processes involved in designing the pressure sensors are outlined, with Hall effect transistors being selected. These units are compact, offer high sensitivity and are inexpensive. An initial prototype acrylic palate was constructed with five embedded pressure sensors. Syllable repetitions were recorded from one adult female. MAIN OUTCOMES, RESULTS AND FUTURE DIRECTIONS: The pressure-sensing palate was capable of recording dynamic tongue-to-palate pressures, with minimal to no interference to speech detected perceptually. With a restricted number of sensors, problems were encountered in optimally positioning the sensors to detect the consonant lingual pressures. Further developments are planned for various aspects of the pressure-sensing system. CONCLUSIONS: Although only in the prototype stage, the pressure-sensing palate represents the new generation of EPG. Comprehensive analysis of tongue-to-palate contacts, including pressure measures, is expected to enable more specific and effective therapeutic techniques to be developed for a variety of speech disorders.

Reflection of structural waves at a solid/liquid interface.

This paper investigates the reflection characteristics of structural or guided waves in rods at a solid/liquid interface. Structural waves, whose wavelengths are much larger than the diameter of the rod, are described in a first approximation by classical one-dimensional wave theory. The reflection characteristics of such waves at a solid/liquid (melting) interface has been reported by two different ultrasonic measurement techniques: first, measuring the fast regression rate of a melting interface during the burning of metal rod samples in an oxygen-enriched environment, and second, monitoring the propagation of the solid/liquid interface during the slow melting and solidification of a rod sample in a furnace. The second work clearly shows that the major reflection occurs from the solid/liquid interface and not the liquid/gas interface as predicted by plane longitudinal wave reflectivity theory. The present work confirms this observation by reporting on the results of some specially designed experiments to identify the main interface of reflection for structural waves in rods. Hence, it helps in explaining the fundamental discrepancy between the reflection characteristics at a solid/liquid interface between low frequency structural waves and high frequency bulk waves, and confirms that the detected echo within a burning metallic rod clearly represents a reflection from the solid/liquid interface.