Bipedal locomotion in Tropidurus torquatus (Wied, 1820) and Liolaemus lutzae Mertens, 1938.

Bipedalism has evolved on numerous occasions in phylogenetically diverse lizard families. In this paper we describe, for the first time, bipedal locomotion on South American lizards, the sand-dweller Liolaemus lutzae and the generalist Tropidurus torquatus. The lizards were videotaped running on a racetrack and the sequences were analyzed frame by frame. The body posture, as a whole, diverged a lot during bipedal locomotion between the two species, even though there was no difference regarding their sprint performance. The locomotor behavior of L. lutzae is, in general, more similar to the one observed on other sand-dweller lizards. Certain particularities are common, such as the digitigrade posture at footfall and throughout stance, trunk angles; and tail posture. In contrast, T. torquatus exhibited high trunk angles and dragged its tail, in a posture compared to basilisks. This body posture could be related to certain characteristics and obstacles of a microhabitat such as the one around lakes and streams (basilisks) and the one with compact shrubby vegetation (T. torquatus).

Bipedal locomotion in Tropidurus torquatus (Wied, 1820) and Liolaemus lutzae Mertens, 1938 / Bipedalismo em Tropidurus torquatus (Wied, 1820) e Liolaemus lutzae Mertens, 1938

Bipedalism has evolved on numerous occasions in phylogenetically diverse lizard families. In this paper we describe, for the first time, bipedal locomotion on South American lizards, the sand-dweller Liolaemus lutzae and the generalist Tropidurus torquatus. The lizards were videotaped running on a racetrack and the sequences were analyzed frame by frame. The body posture, as a whole, diverged a lot during bipedal locomotion between the two species, even though there was no difference regarding their sprint performance. The locomotor behavior of L. lutzae is, in general, more similar to the one observed on other sand-dweller lizards. Certain particularities are common, such as the digitigrade posture at footfall and throughout stance, trunk angles; and tail posture. In contrast, T. torquatus exhibited high trunk angles and dragged its tail, in a posture compared to basilisks. This body posture could be related to certain characteristics and obstacles of a microhabitat such as the one around lakes and streams (basilisks) and the one with compact shrubby vegetation (T. torquatus).

O bipedalismo apareceu em inúmeras ocasiões em famílias de lagartos filogeneticamente diversas. Neste trabalho, descrevemos, pela primeira vez, locomoção bipedal em lagartos sul-americanos, o lagarto de hábito terrestre Liolaemus lutzae e o generalista Tropidurus torquatus. Os lagartos foram filmados correndo em uma pista de corrida e as seqüências foram analisadas quadro a quadro. A postura corporal, como um todo, divergiu bastante durante a locomoção bipedal das duas espécies, mesmo quando não houve diferença significativa em relação ao desempenho locomotor. O comportamento locomotor de L. lutzae é, em geral, similar ao observado em outros lagartos especialistas em solos arenosos. Certas particularidades são comuns, como a postura digitígrada no momento de apoio e durante a fase de apoio; ângulos do corpo e postura da cauda. Em contraste, T. torquatus exibiu altos ângulos corporais e arrastava sua cauda, numa postura comparada à dos lagartos basiliscos. Este tipo de postura corporal pode estar relacionado com certas características e obstáculos de um microhabitat como ao redor de rios e riachos (basiliscos) e uma vegetação de arbustos compacta (T. torquatus).

The growing competition in Brazilian science: rites of passage, stress and burnout

Brazil's scientific community is under pressure. Each year there is an increase in its contribution to international science and in the number of students who are trained to do research and teach at an advanced level. Most of these activities are carried out in state and federal universities, but with government funding that has decreased by more than 70 percent since 1996. Interviews with graduate students, post-doctoral fellows and professors in one university department with a strong research tradition illustrate the level of stress engendered by the conflict between increasing competition and diminishing resources, and serve to underscore the negative effects on creativity and on the tendency to choose science as a career

The growing competition in Brazilian science: rites of passage, stress and burnout.

Brazil's scientific community is under pressure. Each year there is an increase in its contribution to international science and in the number of students who are trained to do research and teach at an advanced level. Most of these activities are carried out in state and federal universities, but with government funding that has decreased by more than 70% since 1996. Interviews with graduate students, post-doctoral fellows and professors in one university department with a strong research tradition illustrate the level of stress engendered by the conflict between increasing competition and diminishing resources, and serve to underscore the negative effects on creativity and on the tendency to choose science as a career.

Teste de DNA para verificaçäo de parentesco em cäes: avaliaçäo do método näo automatizado com o auxílio do primer CMR S / DNA test for parentage verification in dogs: evaluation of the non-automatized method with assistance of the primer CMR S

To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and Säo Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use

Microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificaçäo de parentesco para bovinos da raça Gir / Microsatellites BM2113, ILSTS005, ILSTS008, ETH131 and RM88 for parentage tests in bovines of Gir breed

Foram utilizados 46 animais da raça Gir, registrados na Associaçäo Brasileira de Criadores de Zebu, provenientes de cinco fazendas situadas no Estado de Minas Gerais, com o objetivo de avaliar a eficiência dos microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificaçäo de parentesco. Os locos BM2113, ILSTS005, ETH131 e RM88 mostraram-se eficientes, apresentando valores de PE2 (probabilidade de exclusäo quando os dois progenitores säo genotipados) entre 0,62 e 0,69 e PIC2 (conteúdo de informaçäo polimórfica quando os dois progenitores säo genotipados) entre 0,78 e 0,83. O mesmo näo ocorreu para o loco ILSTS008, o qual apresentou baixos valores de PE2 (0,24) e PIC2 (0,41)

Estudo de famílias de touros da raça Gir e identificaçäo de novos fenogrupos do sistema B e de grupos sangüíneos / Gir breed sire family analyses and identification of new phenogroups of the B system blood group

Foram analisados testes de tipagem sangüínea de 91 animais da raça Gir, pertencentes a um rebanho altamente selecionado para produçäo leiteira. Pelo método de famílias de touros foram estudadas as progênies dos três principais touros fundadores do rebanho. Três novos fenogrupos foram identificados e suas freqüências na amostra foram: B(P)QTE'3G'P', 17,03 por cento; BGKO1TY2D'E'1, 10,98 por cento e BGKY2A'(B')D'E'3G'P'Q', 8,79 por cento

Longitudinal fecal steroid excretion in maned wolves (Chrysocyon brachyurus).

This study used a fecal steroid monitoring technique to evaluate reproductive cycles in male (4) and female (15) maned wolves, endangered South American canids. A radiolabeled testosterone infusion on a male revealed a fast and predominantly fecal route of excretion for this steroid. Testosterone was also excreted as eight unidentified metabolites, which was not the primary form of this steroid quantified in our assays. Fecal steroid concentrations (estradiol, E2; progestins, P; testosterone, T) in males and acyclic, nonpregnant (pseudo-pregnant), and pregnant females were monitored over four breeding seasons (October-January). Significant differences were detected between longitudinal P profiles of cyclic and acyclic females during estrus, luteal phase, and after birth/end of pseudo-pregnancy. Concentrations of P were also significantly higher in pregnant, compared to nonpregnant females, from proestrus to the end of the pregnant luteal phase. Although levels of T were higher in males than in females throughout the breeding season, no cyclicity in male fecal T concentrations was detected. Values of fecal P, T, and the ratio P/T were useful for differentiating gender and detecting pregnancy in females. Similarities to available data on other canids and the management and conservation implications of these findings were discussed.

[Percutaneous endoscopic gastrostomy and gastrojejunostomy. Experience and its role in domiciliary enteral nutrition]. / La gastrostomía y gastroyeyunostomía endoscópica percutánea. Experiencia y su papel en la nutrición enteral domiciliaria.

UNLABELLED: Percutaneous Endoscopic Gastrostomy (PEG) and its variation Percutaneous Endoscopic Gastrojejunostomy (PEGJ), has become the method of choice to achieve an enteral access route in patients who require long term enteral nutrition, especially in the area of the At Home Enteral Nutrition (AHEN). We present our experience on the first PEG's and PEGJ's carried out in our hospital. MATERIAL: We studied 48 patients (14 women and 34 men) in whom a PEG/Percutaneous Endoscopic Gastrojejunostomy (PEGJ) was indicated, as they required enteral nutrition for prolonged periods of time (> 4 weeks) and/or they presented obstructive dysphagia, neuromotor dysphagia, or incorrigible vomiting in the two cases in whom PEGJ was carried out. 34 patients underwent the Ponsky-Gauderer technique, 6 patients underwent the Sacks-Vine technique, and 2 patients underwent a PEGJ. 24 hours after the PEGJ enteral nutrition (EN) was begun in a progressive manner. During the hospitalization period there was a daily follow up of the patient. In those cases in which At Home Enteral Nutrition was programmed, the patients/families were trained in the techniques and the care of the PEG and the EN, and the control was carried out through the Nutrition out patient department. RESULTS: PEG was successfully carried out in 42 patients (88%). 35 patients had previously been given EN through a naso-gastric tube (NGT), while in 7 cases the PEG was the first enteral access route. The average duration of the PEG was 212 days, and 27 patients (64%) needed the PEG for more than 3 months. The mean caloric supply was 1921 +/- 200 kcal/day. The mode of administration was by means of an intermittent infusion by gravity in 31 cases, and by continuous infusion using a volumetric pump in 11 patients. Two patients with pregnancy induced hyperemesis underwent a PEGJ in the 3rd and the 4th month of pregnancy, with the pregnancy being successfully brought to term and ending in vaginal deliveries. Carrying out a PEG permitted release from hospital and the programming of At Home Enteral Nutrition in 30 patients. With respect to the evolution of the patients, 22 patients have died during the course of the study. 18 patients remain in follow up in an ambulatory Enteral Nutrition program, and in the two patients with pregnancy induced hyperemesis, the PEGJ was removed after the pregnancy was successfully ended. There were no complications of any kind in 21 patients. The most common complication was the infection of the gastrostomy, which occurred in 13 patients. There was an accidental removal of the gastrostomy tube in 3 patients. In 3 cases there was an eversion of the gastric mucosa through the ostomy within the first 24-hours, and 20 days after the PEG respectively. In 2 cases there was an incarceration of the gastrostomy tube in the abdominal wall. Only two patients showed an important reflux of the gastric contents. There were no deaths as a result of PEG complications. CONCLUSION: From our experience we can conclude the advantages of PEG as a long term nutritional support, showing a low incidence of complications, and the endoscopic technique has a zero mortality.

Enteral nutrition by percutaneous endoscopic gastrojejunostomy in severe hyperemesis gravidarum: a report of two cases.

We describe the first two cases in which percutaneous endoscopic gastrojejunostomy was used as a means to provide enteral nutrition in severe hypermesis gravidarum. The use of this method of enteral access provided an alternative to parenteral nutrition, was well tolerated, cost-effective and had no major complications. In both cases the nutritional goal for mothers as well as appropriate fetal growth and development were achieved.