ABSTRACT
Infectious bronchitis virus isolate (IND/AHL/16/01) from a disease outbreak characterized by nephritis, gout and mortality in coloured layer pureline at Directorate of Poultry Research, India was characterized as nephropathogenic strain by S1 genotyping and phylogenetic analysis. Serotyping with homologous and heterologous serum (M41) by virus neutralization assay in embryonated chicken eggs (ECE) showed indices of 7.3 and 2.3 respectively. Pathogenesis, tissue tropism and host immune response induced by this isolate were investigated in experimentally infected chicken. A total of 150, twenty days old seronegative Vanaraja birds were inoculated through intranasal and intravenous route using 104.7 Embryo infective dose50 (EID50/ml). Infected chickens were sacrificed at 4 h, 1, 2, 3, 5, 7, 11, 13, 15- and 20-days post-infection (dpi) for necropsy. Tissues were collected for histopathology and virus detection by isolation in ECE and by reverse transcription- PCR (RT-PCR). Serum was also collected at these intervals to investigate the specific antibody response induced. The symptoms started as early as 3 dpi and included primarily wet droppings, diarrhoea, dehydration rather than respiratory symptoms. Gross lesions were prominent in kidneys including mottling and congestion. Virus isolation and RT-PCR detection indicated the presence of virus as early as 4 h post-infection in trachea and 24 h in kidney and lungs and from 2 dpi in caecal tonsil. The host antibody response after experimental infection in serum by ELISA indicated that the protective titres were induced from 13 dpi and peaked at 35 dpi and declined thereafter. Overall, this isolate is nephropathogenic and capable of inducing severe nephritis and production loss in broilers. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13337-021-00693-4.