Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil.

The present study aimed to detect molecularly the presence of co-infections by vector-borne agents (VBA) in ring-tailed coatis' (Nasua nasua) blood samples from Iguaçu National Park (INP), southern Brazil, and assess the phylogenetic positioning of the detected agents. DNA blood samples were submitted to molecular screening and characterization for Anaplasmataceae agents, Piroplasmids, Hepatozoon sp., hemotropic mycoplasmas, and Bartonella spp. In total, 42 (85.7%) coatis were positive for hemotropic Mycoplasma sp., 12 (24.5%) for Bartonella machadoae, 7 (14.3%) for Anaplasma sp. closely related to 'Candidatus Anaplasma brasiliensis', and 3 (6%) for Hepatozoon procyonis. The most prevalent co-infections observed was from bacterial VBA: while 18.3% were co-infected by hemotropic Mycoplasma sp. and Bartonella sp., 12.2% were co-infected by Anaplasma sp. and hemotropic Mycoplasma sp. Only two animals (4%) presented co-infections by three VBA (Bartonella sp., Anaplasma sp. and hemotropic Mycoplasma sp.). The coati is a wild carnivore found in INP, mainly in areas visited by tourists. These animals are frequently seen searching for food in garbage dumps or in tourists' belongings. The present study expands the host specificity range of B. machadoae, which has been isolated only from rodents until the present moment. Since the zoonotic potential and transmission routes of the detected VBA are not yet known, surveillance in this area is much needed.

Short-term physical exercise controls age-related hyperinsulinemia and improves hepatic metabolism in aged rodents.

PURPOSE: Aging is associated with changes in glucose homeostasis related to both decreased insulin secretion and/or impaired insulin action, contributing to the high prevalence of type 2 diabetes (T2D) in the elderly population. Additionally, studies are showing that chronically high levels of circulating insulin can also lead to insulin resistance. In contrast, physical exercise has been a strategy used to improve insulin sensitivity and metabolic health. However, the molecular alterations resulting from the effects of physical exercise in the liver on age-related hyperinsulinemia conditions are not yet fully established. This study aimed to investigate the effects of 7 days of aerobic exercise on hepatic metabolism in aged hyperinsulinemic rats (i.e., Wistar and F344) and in Slc2a4+/- mice (hyperglycemic and hyperinsulinemic mice). RESULTS: Both aged models showed alterations in insulin and glucose tolerance, which were associated with essential changes in hepatic fat metabolism (lipogenesis, gluconeogenesis, and inflammation). In contrast, 7 days of physical exercise was efficient in improving whole-body glucose and insulin sensitivity, and hepatic metabolism. The Slc2a4+/- mice presented significant metabolic impairments (insulin resistance and hepatic fat accumulation) that were improved by short-term exercise training. In this scenario, high circulating insulin may be an important contributor to age-related insulin resistance and hepatic disarrangements in some specific conditions. CONCLUSION: In conclusion, our data demonstrated that short-term aerobic exercise was able to control mechanisms related to hepatic fat accumulation and insulin sensitivity in aged rodents. These effects could contribute to late-life metabolic health and prevent the development/progression of age-related T2D.

Treatment of bladder dysfunction with solifenacin: is there a risk of dementia or cognitive impairment?

The use of bladder antimuscarinics is very common in the elderly. However, recent population-based studies that assessed the use of anticholinergics or bladder antimuscarinics showed an increased risk of dementia when these drugs were used for a prolonged period. Several of these population-based studies included patients who used solifenacin, which is a bladder antimuscarinic released in 2005 with the prospect of being a more selective antimuscarinic for M3 receptors (M3R), which could make it a safer drug when trying to avoid unwanted effects of older bladder antimuscarinics such as oxybutynin, especially with regard to changes in cognition. Since the various bladder antimuscarinics have distinct pharmacological characteristics, such as in the ability to penetrate the blood-brain barrier, in selectivity for muscarinic receptors, and in brain efflux mechanisms, their effects on the central nervous system (CNS) may vary. Solifenacin was the drug selected in this review, which aims to describe the results of several articles published in recent years reporting the effects of solifenacin on cognition or the risk of dementia development. Although preclinical studies show that solifenacin can also act on brain M1 receptors (M1R), short-term clinical studies have shown it to be safe for cognition. However, there are no long-term randomized studies that prove the safety of this drug for the CNS. Thus, until the safety of solifenacin has been established by long-term studies, it seems advisable to avoid prolonged use of this drug in elderly patients.

Treatment of bladder dysfunction with solifenacin: is there a risk of dementia or cognitive impairment?

The use of bladder antimuscarinics is very common in the elderly. However, recent population-based studies that assessed the use of anticholinergics or bladder antimuscarinics showed an increased risk of dementia when these drugs were used for a prolonged period. Several of these population-based studies included patients who used solifenacin, which is a bladder antimuscarinic released in 2005 with the prospect of being a more selective antimuscarinic for M3 receptors (M3R), which could make it a safer drug when trying to avoid unwanted effects of older bladder antimuscarinics such as oxybutynin, especially with regard to changes in cognition. Since the various bladder antimuscarinics have distinct pharmacological characteristics, such as in the ability to penetrate the blood-brain barrier, in selectivity for muscarinic receptors, and in brain efflux mechanisms, their effects on the central nervous system (CNS) may vary. Solifenacin was the drug selected in this review, which aims to describe the results of several articles published in recent years reporting the effects of solifenacin on cognition or the risk of dementia development. Although preclinical studies show that solifenacin can also act on brain M1 receptors (M1R), short-term clinical studies have shown it to be safe for cognition. However, there are no long-term randomized studies that prove the safety of this drug for the CNS. Thus, until the safety of solifenacin has been established by long-term studies, it seems advisable to avoid prolonged use of this drug in elderly patients.

Seroepidemiology of Neospora caninum among goats (Capra hircus) in the state of Paraíba, northeastern Brazil / Soroepidemiologia de Neospora caninum em caprinos (Capra hircus) do estado da Paraíba, Nordeste do Brasil

Although goat dairy farms in Brazil may have a higher risk of infection by Neospora caninum than beef farms, risk factor evaluation on a representative population remains to be fully established in Brazil. Accordingly, this study aimed to establish the occurrence of anti-N. caninum antibodies and factors associated with exposure in 406 blood samples from five dairy and three beef goat farms in the state of Paraíba, northeastern Brazil. Anti-N. caninum antibodies were detected by indirect immunofluorescence assay (IFA), with samples considered positive when reacting with dilution ≥ 1:50. A total of 106/406 goats (26.11%; 95% CI: 21.96-30.72%) were seroreactive comprising 2/61 (3.28%), 10/45 (22.22%), 13/50 (26.00%), 17/51 (33.33%) to 29/46 (63.04%) in dairy farms, and from 3/54 (5.56%), 12/50 (24.00%) to 20/49 (40.82%) on the beef farms. No significant associations were found in relation to age, gender, dairy versus beef farms, occurrence of abortions or mummified fetuses, and seroreactivity to N. caninum (P>0.05). In conclusion, goat farms in the state of Paraíba showed the highest occurrence of anti-N. caninum antibodies to date in Brazil.(AU)

Embora as criações caprinas de leite no Brasil possam ter maior probabilidade de risco de infecção por Neospora caninum do que as de carne, a avaliação dos fatores de risco em uma população representativa ainda não está totalmente estabelecida no Brasil. Dessa forma, este estudo teve por objetivo estabelecer a soroprevalência de N. caninum e seus fatores associados à exposição em 406 amostras de sangue de cinco fazendas de leite e três de corte provenientes do estado da Paraíba, região Nordeste do Brasil. A detecção de anticorpos anti-N. caninum foi realizada utilizando-se a reação de imunofluorescência indireta (RIFI), com as amostras consideradas positivas na diluição ≥ 1:50. No total, 106/406 (26,11%; IC 95%: 21,96-30,72%) caprinos foram sororreagentes, variando de 2/61 (3,28%), 10/45 (22,22%), 13/50 (26,00%), 17/51 (33,33%) a 29/46 (63,04%) em fazendas de leite, e de 3/54 (5,56%), 12/50 (24,00%) a 20/49 (40,82%) em fazendas de corte. Não foram observadas associações significativas entre idade, sexo, criação de leite e carne, ocorrência de abortamentos ou fetos mumificados e sororreatividade para N. caninum (P>0,05). Em conclusão, fazendas de caprinos da Paraíba mostraram as mais altas ocorrências de anticorpos anti-N. caninum até o momento no Brasil.(AU)

Seroepidemiology of Neospora caninum among goats (Capra hircus) in the state of Paraíba, northeastern Brazil / Soroepidemiologia de Neospora caninum em caprinos (Capra hircus) do estado da Paraíba, Nordeste do Brasil

Although goat dairy farms in Brazil may have a higher risk of infection by Neospora caninum than beef farms, risk factor evaluation on a representative population remains to be fully established in Brazil. Accordingly, this study aimed to establish the occurrence of anti-N. caninum antibodies and factors associated with exposure in 406 blood samples from five dairy and three beef goat farms in the state of Paraíba, northeastern Brazil. Anti-N. caninum antibodies were detected by indirect immunofluorescence assay (IFA), with samples considered positive when reacting with dilution ≥ 1:50. A total of 106/406 goats (26.11%; 95% CI: 21.96-30.72%) were seroreactive comprising 2/61 (3.28%), 10/45 (22.22%), 13/50 (26.00%), 17/51 (33.33%) to 29/46 (63.04%) in dairy farms, and from 3/54 (5.56%), 12/50 (24.00%) to 20/49 (40.82%) on the beef farms. No significant associations were found in relation to age, gender, dairy versus beef farms, occurrence of abortions or mummified fetuses, and seroreactivity to N. caninum (P>0.05). In conclusion, goat farms in the state of Paraíba showed the highest occurrence of anti-N. caninum antibodies to date in Brazil.(AU)

Embora as criações caprinas de leite no Brasil possam ter maior probabilidade de risco de infecção por Neospora caninum do que as de carne, a avaliação dos fatores de risco em uma população representativa ainda não está totalmente estabelecida no Brasil. Dessa forma, este estudo teve por objetivo estabelecer a soroprevalência de N. caninum e seus fatores associados à exposição em 406 amostras de sangue de cinco fazendas de leite e três de corte provenientes do estado da Paraíba, região Nordeste do Brasil. A detecção de anticorpos anti-N. caninum foi realizada utilizando-se a reação de imunofluorescência indireta (RIFI), com as amostras consideradas positivas na diluição ≥ 1:50. No total, 106/406 (26,11%; IC 95%: 21,96-30,72%) caprinos foram sororreagentes, variando de 2/61 (3,28%), 10/45 (22,22%), 13/50 (26,00%), 17/51 (33,33%) a 29/46 (63,04%) em fazendas de leite, e de 3/54 (5,56%), 12/50 (24,00%) a 20/49 (40,82%) em fazendas de corte. Não foram observadas associações significativas entre idade, sexo, criação de leite e carne, ocorrência de abortamentos ou fetos mumificados e sororreatividade para N. caninum (P>0,05). Em conclusão, fazendas de caprinos da Paraíba mostraram as mais altas ocorrências de anticorpos anti-N. caninum até o momento no Brasil.(AU)

Atypical Toxoplasma gondii genotype in feral cats from the Fernando de Noronha Island, northeastern Brazil.

Toxoplasma gondii isolates from Brazil have a different phenotypic and genotypic pattern, with predominance of virulent isolates and recombinant genotypes, compared to the North Hemisphere. Considering that a new T. gondii genotype, non-pathogenic to mice, was previously identified from free-range chickens from the Fernando de Noronha Island, Brazil, this study aimed to identify genotypes of this parasite in tissue samples of feral cats (Felis catus) from this Brazilian Island. Anti-T. gondii IgG antibodies were detected in 18/31 (58%) feral cats. Two non-virulent T. gondii isolates were obtained by mouse bioassay. Genotyping was performed by PCR-RFLP using 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico) and an atypical strain of T. gondii (ToxoDB #146) was identified. This is the first report of this genotype in feral cats.

Similar Aggressiveness of Phenotypically and Genotypically Distinct Isolates of Sclerotinia sclerotiorum.

Understanding how Sclerotinia sclerotiorum aggressiveness varies among isolates may be useful for breeding programs aimed at developing common bean cultivars resistant to white mold. The aggressiveness of 20 S. sclerotiorum isolates collected in common bean fields from four Brazilian states was tested against two common bean genotypes (Pérola and A195) using two inoculation methods. The isolates were characterized using 10 microsatellite (SSR) loci, mycelial compatibility groups (MCGs), partial sequences of the oxaloacetate acetylhydrolase (OAH) gene, and morphological traits. Twenty SSR and seven OAH haplotypes, 10 MCGs, and high variability in colony morphology were found. One isolate was more aggressive when inoculated on plants of the genotype A195, but all other isolates had similar aggressiveness. Aggressiveness was not related with MCGs, SSR, OAH haplotypes, mycelial pigmentation, growth rate, or sclerotia production.

Adaptation and Resistance to Diseases in Brazil of Putative Sources of Common Bean Resistance to White Mold.

Common bean breeding programs for white mold (WM) resistance are in their initial stages in Brazil. Sources of partial resistance to WM are available abroad but their performance in Brazil is unknown. In two greenhouse (straw test) and three field experiments conducted in three districts in the state of Minas Gerais, Brazil, we evaluated a total of 23 lines with putative WM resistance with the objective to select lines with resistance to WM and other diseases associated with high yield potential. Two field-resistant local lines, two susceptible local cultivars, and two susceptible international lines were also included in the study. In the greenhouse, Cornell 605, A 195, and G122 were among the lines with the highest partial resistance to WM. In the field, these three lines were highly resistant to WM and had intermediate resistance or were resistant to anthracnose, angular leaf spot, rust, and Fusarium wilt. Cornell 605 and A 195 had high-yield potential but G122 yielded 47% less than the local lines under WM pressure. Our results suggest that Cornell 605 and A 195 are the most useful sources of resistance to WM for use in common bean breeding programs in Brazil.

Fungicide Sensitivity of Sclerotinia sclerotiorum: A Thorough Assessment Using Discriminatory Dose, EC50, High-Resolution Melting analysis, and Description of New Point Mutation Associated with Thiophanate-Methyl Resistance.

Thiophanate-methyl (TM), fluazinam, and procymidone are fungicides extensively used for white mold control of common bean in Brazil. We assessed the sensitivity of Brazilian isolates of Sclerotinia sclerotiorum to these three fungicides using discriminatory doses and concentration that results in 50% mycelial growth inhibition (EC50) values. In total, 282 isolates from the most important production areas were screened and none was resistant to fluazinam or procymidone. The EC50 values varied from 0.003 to 0.007 and from 0.11 to 0.72 µg/ml for fluazinam and procymidone, respectively. One isolate was resistant to TM. The EC50 of the TM-resistant isolate was greater than 100 µg/ml, whereas the EC50 of the sensitive isolates varied from 0.38 to 2.23 µg/ml. The TM-resistant isolate had a L240F mutation in the ß-tubulin gene. This is the first report of mutation at codon 240 causing resistance to a benzimidazole fungicide in S. sclerotiorum. The high-resolution melting analysis allowed the distinction of TM-sensitive and -resistant isolates by specific melting peaks and curves. The TM-resistant isolate had mycelial growth, sclerotia production, and aggressiveness comparable with that of the sensitive isolates, indicating that this genotype will likely compete well against sensitive isolates in the field. This study demonstrates that resistance to TM, fluazinam, and procymidone is nonexistent or rare. Resistance management practices should be implemented, however, to delay the spread of TM-resistant genotypes.

Management of Anthracnose in Common Bean by Foliar Sprays of Potassium Silicate, Sodium Molybdate, and Fungicide.

This study aimed to determine whether foliar sprays of potassium silicate (KSi), sodium molybdate (NaMo), or a combination of both (KSi + NaMo), with or without the fungicide azoxystrobin (Azox), could reduce anthracnose symptoms and, consequently increase yield. Two two-by-four factorial experiments, consisting of untreated or fungicide treated, as well as sprays of KSi, NaMo, KSi + NaMo, and no spray (control), were arranged in a randomized block design with three replications. Treatments were as follows: treatment 1, KSi spray; treatment 2, NaMo spray; treatment 3, KSi + NaMo spray; treatment 4, Azox spray; treatment 5, Azox + KSi spray; treatment 6, Azox + NaMo spray, treatment 7, Azox + KSi + NaMo spray; and treatment 8, control (no KSi, NaMo, or Azox). The KSi, NaMo, and Azox treatments were sprayed at the rates of 35 g/liter, 90 g/ha, and 120 g a.i./ha, respectively. The KSi was applied at 20, 27, 40, and 55 days after sowing (das). The NaMo was sprayed only at 27 das whereas the fungicide was sprayed at 27, 40, and 55 das. Plants were inoculated with Colletotrichum lindemuthianum at 23 das. Azox reduced the mean area under disease progress curve (AUDPC) by 63% and mean yield was increased by 150%. Similarly, the mean AUDPC was reduced by 29, 14, and 41% with KSi, NaMo, and KSi + NaMo sprays, respectively, while mean yield increased by 13, 20, and 47%, with KSi, NaMo, or KSi + NaMo sprays, respectively. The variables leaf area index (LAI), leaf area index duration (LAD), healthy leaf area duration (HAD), and radiation intercepted (RI) were not affected by KSi spray. The values for the variables LAI, healthy leaf area index (HLAI), LAD, HAD, RI, intercepted radiation of the healthy leaf area, and healthy leaf area absorption were significantly increased as a result of NaMo spray. The results of the present study support the novel possibility of using a foliar spray of KSi in association with NaMo to decrease anthracnose symptoms in bean plants and, consequently, achieve greater yield.

Carbamazepine inhibits angiotensin I-converting enzyme, linking it to the pathogenesis of temporal lobe epilepsy.

We find that a common mutation that increases angiotensin I-converting enzyme activity occurs with higher frequency in male patients suffering from refractory temporal lobe epilepsy. However, in their brains, the activity of the enzyme is downregulated. As an explanation, we surprisingly find that carbamazepine, commonly used to treat epilepsy, is an inhibitor of the enzyme, thus providing a direct link between epilepsy and the renin-angiotensin and kallikrein-kinin systems.

A quantitative TaqMan PCR assay for the detection of Mycoplasma suis.

AIM: To develop a TaqMan probe-based, highly sensitive and specific quantitative PCR (qPCR) assay for the detection and quantification of Mycoplasma suis in the blood of pigs. METHODS AND RESULTS: Primers and probes specific to Myc. suis 16S rRNA gene were designed. The qPCR assay's specificity, detection limit, intra- and inter-assay variability were evaluated and its performance was compared with a Myc. suis conventional PCR assay (cPCR). Blood of two experimentally infected pigs, 40 Indiana pigs, 40 Brazilian sows and 28 peccaries were tested. The assay detected as few as ten copies of Myc. suis plasmids and was 100-fold more sensitive than the cPCR. No cross-reactivity with nontarget pig mycoplasmas was observed. An average of 1·62 × 10(11) and 2·75 × 10(8) target copies ml(-1) of blood were detected in the acutely and chronically infected pigs, respectively. Three (7·5%) pigs and 32 (80·0%) sows were positive while all peccaries were negative for Myc. suis. CONCLUSION: The developed qPCR assay is highly sensitive and specific for Myc. suis detection and quantification. SIGNIFICANCE AND IMPACT OF THE STUDY: TaqMan qPCR is an accurate and quick test for detection of Myc. suis infected pigs, which can be used on varied instrumentation platforms.

Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs.

Canine visceral leishmaniasis (CVL) is caused by Leishmania donovani complex parasites including L. donovani, Leishmania infantum and Leishmania chagasi. As some studies suggest that L. chagasi and L. infantum may be very similar or even the same species, the aim of the present study was to evaluate a commercial rapid ELISA test, originally designed for L. infantum, in the diagnosis of CVL in dogs naturally infected by L. chagasi. A total of 400 serum canine samples, including 283 positive dogs for CVL from an endemic area, 86 clinically healthy dogs from a non-endemic area and 31 dogs seropositive for confounding infectious agents (Trypanosoma cruzi, Toxoplasma gondii, Neospora caninum, Babesia canis and Ehrlichia canis) were used for test validation. An overall sensitivity of 94.7% (95% CI=91.41-97.01%) and specificity of 90.6% (95% CI=83.80-95.21%) was found, with a high degree of agreement (k=0.8445) to the indirect ELISA. When confounding infectious diseases were excluded, specificity increased to 100% (95% CI=95.8-100%), with a higher degree of agreement (k=0.8928). In conclusion, the commercial kit designed for L. infantum was a highly sensitive and specific device for detection of L. chagasi infection in dogs, which indicates high immunoreactivity similarities between L. infantum and L. chagasi.

Propagação vegetativa de Brosimum gaudichaudii Tréc. (mama-cadela) por estacas de raízes / Vegetative propagation of Brosimum gaudichaudii Tréc. (mama-cadela) by root cuttings

Brosimum gaudichaudii Tréc. (mama-cadela) é uma planta medicinal nativa do Cerrado, utilizada na medicina tradicional. O objetivo deste trabalho foi verificar a possibilidade de propagação de mama-cadela por meio de estacas de raízes, sob o efeito de reguladores de crescimento e de diferentes substratos. No experimento 1 foram avaliados os efeitos da aplicação de ácido indol-butírico (AIB) e ácido naftaleno-acético (ANA) na concentração de 1000 mg L-1 e três substratos (S1- areia; S2 - 75 por cento de areia + 25 por cento de substrato comercial; S3 - 50 por cento de areia + 50 por cento de substrato comercial). No experimento 2 foram avaliadas 4 doses de AIB: 0 - testemunha; 250 mg L-1 (1,3426 mM ); 500 mg L-1 (2,6853 mM) e 1000 mg L-1 (5,3706 mM); e 4 doses de ácido naftaleno-acético (ANA): 0 - testemunha; 250 mg L-1 (1,2295 mM); 500 mg L-1 (2,458 mM); e 1000 mg L-1 (4,918 mM). O delineamento experimental utilizado foi de blocos ao acaso com três repetições de seis estacas por parcela. No experimento 1, os substratos compostos por areia e areia (75 por cento) + substrato comercial (25 por cento) proporcionaram os maiores Índices de pegamento. A aplicação de AIB (1000 mg L-1) proporcionou aumentos relativos de 30,8 por cento e 51,3 por cento, no IMP quando comparada com a testemunha e a aplicação de ANA, respectivamente. No experimento 2, observou-se resposta quadrática significativa da aplicação de AIB sobre o IMP. A dose de 500 mg L-1 (2,6853 mM) promoveu maior IMP. Não houve efeito significativo das doses de ANA para os parâmetros avaliados. Não houve efeito significativo relevante de substratos ou hormônios sobre os demais parâmetros avaliados. Estes resultados evidenciam o potencial de utilização de estacas de raiz de mama-cadela para a produção de mudas clonais desta espécie.

Brosimum gaudichaudii Tréc. (mama-cadela) is a medicinal plant native to Cerrado and largely used in traditional medicine. The aim of this study was to verify the propagation of mama-cadela by means of root cutting under the effect of plant growth regulators and different substrates. In experiment 1, the effects of indole butyric acid (IBA) and naphthylacetic acid (NAA) at the concentration of 1000 mg L-1 were evaluated together with three substrates (S1 - sand; S2 - 75 percent sand + 25 percent commercial substrate; S3 - 50 percent sand + 50 percent commercial substrate). In experiment 2, 4 IBA levels were evaluated: 0 - control; 250 mg L-1 (1.3426 mM ); 500 mg L-1 (2.6853 mM) and 1000 mg L-1 (5.3706 mM), as well as 4 NAA levels: 0 - control; 250 mg L-1 (1.2295 mM); 500 mg L-1 (2.458 mM) and 1000 mg L-1 (4.918 mM). The adopted experimental design was in randomized blocks with three replicates of six cuttings per plot. In experiment 1, substrates containing sand and sand (75 percent) + commercial substrate (25 percent) promoted the highest average rooting indexes. Application of IBA (1000 mg L-1) led to increases of 30.8 percent and 51.3 percent in the average rooting indexes compared to control and NAA application, respectively. In experiment 2, there was a significant quadratic response of IBA application on the average rooting index. The level of 500 mg L-1 (2.6853 mM) promoted the highest average rooting index. There was no significant effect of NAA levels for the evaluated parameters. Similarly, there was no significant effect of substrates or hormones on the remaining parameters evaluated. These results show the potential use of mama-cadela root cuttings for the production of clonal seedlings of this species.

Avaliação agronômica e química de dezessete acessos de erva-cidreira [Lippia alba (Mill. ) N. E. Brown] - quimiotipo citral, cultivados no Distrito Federal / Agronomic and chemical evaluation of seventeen accessions of "erva-cidreira" [Lippia alba (Mill. ) N. E. Brown] - citral chemotype, cultivated at the Federal District, Brazil

A erva-cidreira [Lippia alba (Mill.) N.E.Brown] é uma espécie nativa utilizada como planta medicinal em todo o Brasil. O citral é responsável pela ação sedativa da erva cidreira, sendo um dos principais componentes do óleo essencial. O objetivo deste trabalho foi avaliar o comportamento agronômico, teor e rendimento de óleo essencial e de citral em 17 acessos de L. alba da coleção da Universidade de Brasília - UnB. O experimento foi realizado no período de 19/02/2005 a 18/03/2006, em latossolo vermelho, no Distrito Federal. O delineamento experimental utilizado foi o de blocos ao acaso, com três repetições, contendo três plantas por parcela. Foram avaliados os parâmetros área foliar, comprimento da haste, produção de massa fresca (folhas e hastes), produção de massa foliar seca, teor de óleo essencial e de citral, rendimento estimado de óleo essencial e de citral por planta, além da caracterização do perfil aromático do óleo essencial. Os acessos L.41 (0,75 por cento), L.45 (0,66 por cento) e L.08 (0,62 por cento) apresentaram os maiores teores de óleo essencial. O acesso L.45 (1,32 g pl-1) apresentou o maior rendimento de óleo, seguido dos acessos L.47 (0,73 g pl-1), L.41 (0,67 g pl-1), L.34 (0,67 g pl-1) e L.33 (0,62 g pl-1). Os acessos apresentaram teores de citral variando de 51,7 por cento (L.34) a 75,1 por cento (L.09), com média de 63,6 por cento e predominância do isômero geranial (36,4 por cento) sobre o neral (27,8 por cento). O acesso L.45 (0,83 g pl-1) apresentou o maior rendimento de citral. Treze acessos apresentaram o quimiotipo citral; três o quimiotipo citral-limoneno e apenas um apresentou o quimiotipo citral-mirceno. Os resultados evidenciam o potencial de produção de citral do acesso L.45, o qual poderá ser utilizado para melhorar a qualidade da matéria prima de erva-cidreira utilizada nos programas de fitoterapia no país ou em programas de melhoramento genético para esta finalidade.

"Erva-cidreira" [Lippia alba (Mill.) N.E.Brown] is a native species used as a medicinal plant all over Brazil. Citral, a major constituent of L. alba essential oil, is responsible for its sedative activity. The aim of this study was to evaluate the agronomic behavior, content and yield of essential oil and citral in 17 accessions of L. alba from the germplasm bank of University of Brasília - UnB, Brazil. The experiment was carried out from 02/19/2005 to 03/18/2006, in red latosol, at the Federal District, Brazil. The adopted experimental design was in randomized blocks, with three replicates and three plants per plot. The following parameters were evaluated: leaf area, stem length, fresh mass (leaves and stems) production, dry leaf mass production, essential oil and citral content, estimated yield of essential oil and citral per plant, besides characterization of the essential oil aromatic profile. Accessions L41 (0.75 percent), L45 (0.66 percent) and L08 (0.62 percent) showed the highest essential oil content. Accessions L45 (1.32 g pl-1) presented the highest essential oil yield, followed by L47 (0.73 g pl-1), L41 (0.67 g pl-1), L34 (0.67 g pl-1) and L33 (0.62 g pl-1). Citral content varied from 51.7 percent (L34) to 75.1 percent (L09), with an average of 63.6 percent and predominance of the isomer geranial (36.4 percent) over neral (27.8 percent). Accession L45 (0.83 g pl-1) showed the highest citral yield. Thirteen accessions presented the chemotype citral; three, the chemotype citral-limonene, and only one had the chemotype citral-myrcene. The results evidenced the potential of accession L45 to produce citral, which can be used to improve the quality of "erva-cidreira" raw material in phytotherapy programs in the country or in breeding programs.

Influência de diferentes meios de cultura sobre o crescimento de Pfaffia glomerata (Spreng. ) Pedersen (Amaranthaceae) para conservação in vitro / Influence of different culture media on the growth of Pfaffia glomerata (Spreng. ) Pendersen (Amaranthaceae) for conservation in vitro

Pfaffia glomerata ocorre em vários estados do Brasil e países limítrofes da região Sul às margens de rios e nas orlas das matas de galerias, é espécie hidrófita e heliófita. As raízes de espécies do gênero Pfaffia são usadas na medicina popular brasileira, especialmente como tônico, afrodisíaco e no controle do diabete. O objetivo deste trabalho foi estabelecer um banco de germoplasma in vitro de Pfaffia glomerata. O experimento em delineamento inteiramente casualizado foi conduzido com seis tratamentos: 1) MS + 2 por cento de sacarose + 4 por cento de sorbitol; 2) MS/2 + 2 por cento de sacarose + 4 por cento de sorbitol; 3) MS + 2 por cento de sacarose + 4 por cento de sorbitol + 2 mg L-1 de pantotenato de cálcio; 4) MS/2 + 2 por cento de sacarose + 4 por cento de sorbitol + 2 mg L-1de pantotenato de cálcio; 5) MS + 2 por cento de sacarose + 3 por cento de manitol + 2 mg L-1de pantotenato de cálcio; 6) MS/2 + 2 por cento de sacarose + 3 por cento de manitol + 2 mg L-1de pantotenato de cálcio. Os resultados obtidos foram submetidos à análise de variância e ao teste de separação de médias de Scott Knott. Os tratamentos um, três e quatro apresentaram, significativamente, o maior número de segmentos nodais por haste, quando comparados com os tratamentos dois, cinco e seis. O tratamento dois foi o mais indicado para a conservação in vitro da espécie por ter promovido menor crescimento das plantas (altura de 3,1±1,9 cm), alto índice de sobrevivência, 100 por cento de explantes com brotação e o maior número de brotos por explante, após seis meses de cultivo. Todas as plântulas produziram raízes e não houve formação de calos, também não ocorreu hiperhidricidade nos tratamentos avaliados. As plantas aclimatizadas apresentaram 100 por cento de sobrevivência no ambiente ex vitro. A manutenção de acessos de P. glomerata no banco de germoplasma in vitro é viável tanto do ponto de vista da conservação quanto economicamente.

Pfaffia glomerata occurs in several states of Brazil and its neighboring countries in the south region at riverbanks and gallery forests. It is a hydrophyte and heliophyte species. The roots of the genus Pfaffia are used in Brazilian folk medicine especially as tonic, aphrodisiac and to control diabetes. The aim of this work was to establish an in vitro germplasm bank for Pfaffia glomerata. The experiment was carried out in completely randomized design with six treatments: 1) DM + 2 percent sucrose + 4 percent sorbitol; 2) DM/2 + 2 percent sucrose + 4 percent sorbitol; 3) DM + 2 percent sucrose + 4 percent sorbitol + 2 mg L-1 calcium pantothenate; 4) DM/2 + 2 percent sucrose + 4 percent sorbitol + 2 mg L-1 calcium pantothenate; 5) DM + 2 percent sucrose + 3 percent mannitol + 2 mg L-1 calcium pantothenate; 6) DM/2 + 2 percent sucrose + 3 percent mannitol + 2 mg L-1 calcium pantothenate. Results were subjected to analysis of variance and Scott Knott test for mean grouping. Treatments 1, 3 and 4 had a significantly larger number of nodal segments per stem, compared to Treatments 2, 5 and 6. Treatment 2 was the most appropriate for the in vitro conservation of this species since it led to the lowest growth (3.1±1.9 cm height), high survival rate, 100 percent explants with sprouting, and the largest number of sprouts per explant after six months of culture. All seedlings produced root and showed no formation of calluses or hyperhydricidity under the evaluated treatments. Acclimatized plants showed 100 percent survival in the ex vitro environment. Maintaining P. glomerata accessions in an in vitro germplasm bank is viable both economically and for conservation.

Conservação in vitro de Cochlospermum regium (Schrank) pilg. - cochlospermaceae sob regime de crescimento mínimo / In vitro conservation of Cochlospermum regium (Schrank) pilg. - cochlospermaceae under minimal growth storage

Cochlospermum regium é uma planta de áreas de cerrado, caatinga e pantanal. Na medicina popular é conhecida por "algodão-do-campo" e suas raízes são utilizadas para o tratamento de infecções uterinas, intestinais, gastrite, úlceras e artrite. Atualmente, o extrativismo e a destruição dos habitats naturais colocaram o algodão-do-campo na lista de espécies medicinais nativas prioritárias para conservação ex situ. O objetivo deste trabalho foi desenvolver uma metodologia para a conservação in vitro do algodão-do-campo e fornecer subsídios para estudos de micropropagação da espécie. Sementes de algodão-do-campo foram testadas quanto à germinação in vitro pela escarificação ou não das sementes em ácido sulfúrico e inoculação em meio de cultura MS. Para a conservação in vitro, segmentos nodais retirados das plântulas germinadas in vitro foram avaliados por 90 dias sob três regimes de temperatura (10, 20, e 25ºC) e em três concentrações de meio WPM (½, ¾ e pleno). Verificou-se que sementes escarificadas apresentaram percentual de germinação in vitro de 93,3 por cento aos 30 dias, valor significativamente superior aos 13,3 por cento observados nas sementes não escarificadas. A conservação da espécie in vitro mostrou-se viável, desde que as culturas sejam mantidas em câmara de crescimento a 20ºC em meio de cultivo ½WPM. Sob estas condições os explantes mantiveram um crescimento mínimo e percentual de sobrevivência de 100 por cento, após três meses de avaliação.

Cochlospermum regium is a plant from cerrado, caatinga and pantanal areas. In popular medicine, it is known as "algodão-do-campo" and its roots are used to treat uterine and intestinal infections, gastritis, ulcers and arthritis. Nowadays, extraction activities and the destruction of natural habitats has made "algodão-do-campo" one of the major native medicinal species for ex situ conservation. The aim of this work was to develop a methodology for the in vitro conservation of "algodão-do-campo", contributing to studies on the micropropagation of this species. In vitro germination was evaluated in "algodão-do-campo" seeds subjected to scarification or not with sulfuric acid and inoculation in MS medium. For in vitro conservation, nodal segments from in vitro-germinated seedlings were evaluated for 90 days at three temperatures (10, 20 and 25ºC) and three WPM medium concentrations (½, ¾ and full-strength). Scarified seeds presented 93.3 percent in vitro germination at 30 days of cultivation, a significantly higher value than the 13.3 percent observed for non-scarified seeds. The in vitro conservation of "algodão-do-campo" showed to be viable once cultures are kept in a growth chamber at 20ºC in ½ WPM medium. Under such conditions, the explants presented a minimal growth and 100 percent survival after three evaluation months.