Glyphosate concentrations in global freshwaters: are aquatic organisms at risk?

Glyphosate is the most used herbicide worldwide. Many studies have reported glyphosate risks to aquatic organisms of different trophic levels. Moreover, evidence suggests flaws in countries' legislation that may imply the non-protection of aquatic species exposed to glyphosate. Therefore, we aimed to investigate glyphosate concentrations in freshwater ecosystems worldwide based on a systematic literature review, to discuss the results considering each country's legislation, and to assess the relative tolerance and risk for aquatic species. Only articles providing in situ concentrations of glyphosate in freshwater systems were included in our study. In total, 73 articles met the inclusion criteria and were used in our analysis. The studies comprised freshwater ecosystems from 21 countries. Most countries evaluated (90%) did not have restrictive legislation for aquatic glyphosate concentrations, resulting in a potential non-protection of aquatic organisms. Glyphosate may pose a moderate to high risk in 95% of the countries investigated, reaching a maximum concentration of 105 mg L-1. Additionally, the risk analysis showed that glyphosate concentrations below 0.1 µg L-1 represent a low risk, whereas glyphosate concentrations above 1 µg L-1, which is below the limit established by some countries' legislation, represent a high risk to aquatic organisms. Therefore, we strongly recommend a revision of the countries' legislation for glyphosate concentration in freshwater systems.

Three-bestseller pesticides in Brazil: Freshwater concentrations and potential environmental risks.

Agricultural production in Brazil is favored by weather conditions and by the large amount of available land. Therefore, currently, Brazil is the second largest exporter of agricultural products globally. Pesticides are widely used in Brazilian crops due to their high efficiency, their low cost, and permissive legislation. However, pesticides tend to reach water resources threatening organisms and the water quality. Thereby, we aimed to review the surface freshwater concentrations of the three-bestseller pesticides in Brazil (glyphosate, 2,4D, and atrazine), and discuss the results with sales, legislation, toxicity and potential risks. For that, we performed a systematic review of quantitative studies of glyphosate, atrazine, and 2,4D in Brazilian freshwater and included monitoring data provided by the Brazilian Ministry of Health in our analysis. Finally, we calculated the risk assessment for the three pesticides. Only a few scientific studies reported concentrations of either of the three-bestseller pesticides in Brazilian freshwaters. Between 2009 and 2018, an increase in the sales of 2,4D, atrazine, and glyphosate was observed. It was not possible to evaluate the relation between concentrations and sales, due to limited number of studies, lack of standard criteria for sampling, individual environmental properties, and type of pesticide. Atrazine showed a higher toxicity compared to 2,4D and glyphosate. Regarding the environmental risks, 65%, 72%, and 94% of the Brazilian states had a medium to high risk to 2,4D, atrazine, and glyphosate, respectively. Finally, 80% of the Brazilian states evaluated showed a high environmental risk considering a mixture of the three pesticides. Although most of the environmental concentrations registered were below the allowed limits according to the Brazilian legislation, they are already enough to pose a high risk for the aquatic ecosystems. We, therefore, strongly recommend a revaluation of the maximum allowed values in the national surface freshwater Brazilian legislation.

Graphene-based nanomaterial for desalination of water: A systematic review and meta-analysis.

Due to its unique properties, graphene has emerged as a promising green nanomaterial for many applications. The porous structure and wetting characteristics of graphene make it an excellent and promising nanomaterial for water desalination (WD) and purification. The present work presents a systematic review and performs a meta-analysis on the application of graphene-based nanomaterial for WD. The reported results encourage expanding the use of different types of graphene-based nanomaterial in WD and thus fill the knowledge gaps in finding a comprehensive, simple, and cost-effective application of nanomaterial in WD. The meta-analysis showed that the cheap and simple graphene-based nanomaterial have an effective salt rejection efficiency (%SAR) in the range 83.04 (79.95-96.13); besides, the %SAR for meta-analysis on various desalination techniques is 99.8% (pervaporation), 55% (adsorption), and 100% (distillation). Nonetheless, the present literature review concluded that more focused research works are required: i) to investigate and evaluate the optimal operating conditions, the impact of environmental hazards, techno-economic analysis of the process and ii) to evaluate the probable nano-toxicity that occurs due to the accidental release of nanomaterials.

A global systematic review, meta-analysis, and risk assessment of the concentration of vanadium in drinking water resources.

The presence of toxic metals such as vanadium in water resources has attracted considerable attention as a new concern in international health. Systematic review and meta-analysis were performed to assess the concentration of vanadium in water resources along with the relevant ecological risk assessment. Databases of Scopus, PubMed, and Embase were investigated to retrieve the related articles from January 01, 1974 to December 25, 2019. Twenty-eight articles containing 152 samples from 24 countries were included. Furthermore, the meta-analysis was conducted by the approach of z-score to estimate differences in the effect size. In addition, the mean of concentrations of vanadium was applied to calculate the risk assessment only to the water surface and choose the maximum environmental concentration (MEC) for demonstrate a worst-case scenario. Here, the risk assessment approach was used to show that the MEC of vanadium confirm the risk it for aquatic ecosystems, being fish (e.g., Danio rerio) our model organism due to their sensibility. According to findings, the MEC of vanadium in surface water varied from 0.010 µg L-1 (USA) and 68 µg L-1 (China), with an overall mean of 6.21 ± 13.3 µg L-1 (mean ± standard deviation). The ecological risk assessment demonstrated that people living in some countries such as China and Japan were at an adverse ecological risk of vanadium in the water resources. Hence, essential control plans besides adequate removal techniques must be implemented for significant deracination of heavy metals like vanadium.

Multiple stressors in Mediterranean coastal wetland ecosystems: Influence of salinity and an insecticide on zooplankton communities under different temperature conditions.

Temperature increase, salinity intrusion and pesticide pollution have been suggested to be among the main stressors affecting the biodiversity of coastal wetland ecosystems. Here we assessed the single and combined effects of these stressors on zooplankton communities collected from a Mediterranean coastal lagoon. An indoor microcosm experiment was designed with temperature variation (20 °C and 30 °C), salinity (no addition, 2.5 g/L NaCl) and the insecticide chlorpyrifos (no addition, 1 µg/L) as treatments. The impact of these stressors was evaluated on water quality variables and on the zooplankton comunity (structure, diversity, abundance and taxa responses) for 28 days. This study shows that temperature is the main driver for zooplankton community change, followed by salinity and chlorpyrifos. The three stressors contributed to a decrease on zooplankton diversity. The increase of temperature contributed to an increase of zooplankton abundance. Salinity generally affected Cladocera, which resulted in a Copepoda increase at 20 °C, and a reduction in the abundance of all major zooplankton groups at 30 °C. The insecticide chlorpyrifos affected primarily Cladocera, altough the magnitude and duration of the direct and indirect effects caused by the insecticide substantially differed between the two temperature scenarios. Chlorpyrifos and salinity resulted in antagonistic effects on sensitive taxa (Cladocera) at 20 °C and 30 °C. This study shows that temperature can influence the direct and indirect effects of salinity and pesticides on zooplankton communities in Mediterranean coastal wetlands, and highlights vulnerable taxa and ecological responses that are expected to dominate under future global change scenarios.

How ATP-Competitive Inhibitors Allosterically Modulate Tyrosine Kinases That Contain a Src-like Regulatory Architecture.

Small molecule kinase inhibitors that stabilize distinct ATP binding site conformations can differentially modulate the global conformation of Src-family kinases (SFKs). However, it is unclear which specific ATP binding site contacts are responsible for modulating the global conformation of SFKs and whether these inhibitor-mediated allosteric effects generalize to other tyrosine kinases. Here, we describe the development of chemical probes that allow us to deconvolute which features in the ATP binding site are responsible for the allosteric modulation of the global conformation of Src. We find that the ability of an inhibitor to modulate the global conformation of Src's regulatory domain-catalytic domain module relies mainly on the influence it has on the conformation of a structural element called helix αC. Furthermore, by developing a set of orthogonal probes that target a drug-sensitized Src variant, we show that stabilizing Src's helix αC in an active conformation is sufficient to promote a Src-mediated, phosphotransferase-independent alteration in cell morphology. Finally, we report that ATP-competitive, conformation-selective inhibitors can influence the global conformation of tyrosine kinases beyond the SFKs, suggesting that the allosteric networks we observe in Src are conserved in kinases that have a similar regulatory architecture. Our study highlights that an ATP-competitive inhibitor's interactions with helix αC can have a major influence on the global conformation of some tyrosine kinases.

Fixação single-point com placa reabsorvível em fratura pediátrica: relato de caso / Single-point fixation with resorbable plate in pediatric fracture: case report

Introdução: As fraturas faciais pediátricas são geralmente decorrentes de traumas de alta energia e o seu tratamento está relacionado à fase do crescimento facial e do padrão do trauma ocorrido. A literatura atual descreve, com sucesso, a fixação de fraturas de zigoma com pouco deslocamento, utilizando apenas um ponto, seja ele na sutura frontozigomática ou no pilar zigomaticomaxilar, podendo ser associado ao uso de placas do sistema de fixação absorvível, principalmente, em pacientes em fase de crescimento. Este trabalho tem por objetivo relatar um caso de fratura de zigoma em paciente pediátrico tratado com placa e parafusos absorvíveis, utilizando o princípio de fixação em um único ponto. Relato de caso: Paciente de 06 anos de idade foi atendido em serviço de Cirurgia Bucomaxilofacial, cursando com ferimento cortocontuso em margem supraorbitária direita associada à presença de degrau palpável em região de sutura frontozigomática direita, o qual foi confirmado pela tomografia computadorizada, demonstrando leve deslocamento medial. O paciente foi submetido à redução aberta e fixação da fratura com material reabsorvível. Considerações finais: A utilização de fixação com placas reabsorvíveis em um único ponto tem sido um excelente aliado, porém não é indicado para fraturas com deslocamento significativo, instáveis e panfaciais... (AU)

Introduction: Pediatric facial fractures are usually consequence of high energy trauma and its treatment is related to the facial growth phase and the trauma itself. The current literature describes, successfully, the fixation of zygoma fracture with little displacement using the single point fixation, being on the frontozygomatic suture or on the zygomatic-maxillary buttress, which can be associated to the use of resorbable fixation system plates, especially in patients growing phase. The aim of this work is report a case of zygomatic fracture in a pediatric patient treated with plate and resorbable screws, using the principle of single-point fixation. Case Report: A 06-year-old patient, attended in the Oral and Maxillofacial Surgery Service, suffering from a cut-contusion wound on the right supraorbital margin associated with the presence of a palpable step in the region of the right frontozygomatic suture, which was confirmed by computed tomography, demonstrating slight medial displacement. The patient was underwent to open reduction and internal fixation of the fracture with resorbable material. Final considerations: The use of fixation with resorbable plates in a single-point has been an excellent approach, however it is not indicated for fractures with significant displacement, unstable and panfacial... (AU)

Ciliates as model organisms for the ecotoxicological risk assessment of heavy metals: A meta-analysis.

Ciliates are key components of aquatic ecosystems, significantly contributing to the decomposition of organic matter and energy transfer to higher trophic levels. They are considered good biological indicators of chemical pollution and relatively sensitive to heavy metal contamination. In this study, we performed a meta-analysis of the available toxicity data of heavy metals and ciliates to assess: (1) the sensitivity of freshwater ciliates to different heavy metals, (2) the relative sensitivity of ciliates in comparison to the standard test species used in ecotoxicological risk assessment, and (3) the difference in sensitivity across ciliate taxa. Our study shows that the tolerance of ciliates to heavy metals varies notably, which is partly influenced by differences in methodological conditions across studies. Ciliates are, in general, sensitive to Mercury > Cadmium > Copper > Zinc > Lead > Chromium. Also, this study shows that most ciliates are more tolerant to heavy metal pollution than the standard test species used in ecotoxicological risk assessments, i.e., Raphidocelis subcapitata, Daphnia magna, and Onchornyncus mykiss. Threshold concentrations derived from toxicity data for these species is expected to confer sufficient protection for the vast majority of ciliate species. Our data analysis also shows that the most commonly tested ciliate species, Paramecium caudatum and Tetrahymena thermophila, are not necessarily the most sensitive ones to heavy metal pollution. Finally, this study stresses the importance of developing standard toxicity test protocols for ciliates, which could lead to a better comprehension of the toxicological impact of heavy metals and other contaminants to ciliate species.

A global trend of caffeine consumption over time and related-environmental impacts.

Caffeine is one of the most consumed substances, and it has been largely detected in aquatic ecosystems. We investigated the trends in caffeine consumption over three decades and its relationships with gross domestic product (GDP) and human development index (HDI) to understand global patterns and to identify potential hotspots of contamination. The total caffeine consumption is increasing mainly due to population growth. Moreover, caffeine consumption per capita is also increasing in some countries, such as Brazil, Italy, and Ethiopia. A high positive correlation between caffeine consumption per capita with HDI and GDP was found for coffee-importing countries in Europe, while a high negative correlation was found for coffee-exporting countries in Africa. The literature review showed that the highest caffeine concentrations coincide with countries that present an increasing caffeine consumption per capita. Also, approximately 35% of the caffeine concentrations reported in the literature were above the predicted no-effect concentration in the environment and, again, overlaps with countries with increasing per capita consumption. Despite the high degradation rate, caffeine consumption tends to increase in a near future, which may also increase the overall amount of caffeine that comes into the environment, possibly exceeding the thresholds of several species described as tolerant to the current environmental concentrations. Therefore, it is essential to prevent caffeine from reaching aquatic ecosystems, implementing sewage treatment systems, and improving their efficiency.

Investigation of medicines consumption and disposal in Brazil: A study case in a developing country.

The incorrect disposal of medicines can be harmful to the environment. Here, we aim to understand the consumption and disposal of medicines in Brazil using online forms. 64% of the respondents have the habit to self-medicate. 66% of respondents dispose the disused or expired medicines in the garbage. 71.9% of respondents never receive any information about correct disposal of medicines. 95.2% of respondents believe that residues of medicines can be harmful to the environment. Environmental education can provide information to the population and help to mitigate pharmaceuticals pollution.