ABSTRACT
Microalgae are the main source of third-generation biofuels because they have a lipid content of 20-70%, can be abundantly produced and do not compete in the food market besides other benefits. Biofuel production from microalgae is a promising option to contribute for the resolution of the eminent crisis of fossil energy and environmental pollution specially in the transporting sector. The choice of lipid extraction method is of relevance and associated to the algae morphology (i.e., rigid cells). Therefore, it is essential to develop suitable extraction technologies for economically viable and environment-friendly lipid recovery processes with the aim of achieving a commercial production of biofuels from this biomass. This review presents an exhaustive analysis and discussion of different methods and processes of lipid extraction from microalgae for the subsequent conversion to biodiesel. Physical methods based on the use of supercritical fluids, ultrasound and microwaves were reviewed. Chemical methods using solvents with different polarities, aside from mechanical techniques such as mechanical pressure and enzymatic methods, were also analyzed. The advantages, drawbacks, challenges and future prospects of lipid extraction methods from microalgae have been summarized to provide a wide panorama of this relevant topic for the production of economic and sustainable energy worldwide.
