Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 57
Filter
1.
Med Oral Patol Oral Cir Bucal ; 28(2): e116-e125, 2023 Mar 01.
Article in English | MEDLINE | ID: mdl-36806025

ABSTRACT

BACKGROUND: This systematic mapping review aims to identify, describe, and organize the currently available evidence in systematic reviews (SR) and primary studies regarding orthognathic surgery (OS) co-interventions and surgical modalities, focusing on the outcomes blood loss, infection and relapse. MATERIAL AND METHODS: A comprehensive search strategy was performed to identify all SRs, randomized controlled trials and observational studies that evaluate surgical modalities and perioperative co-interventions in OS that evaluate the outcomes blood loss, infection and relapse, regardless of language or publication date. Searches were conducted in MEDLINE, EMBASE, Epistemonikos, Lilacs, Web of Science, and CENTRAL. In addition, grey literature was screened. RESULTS: 27 SRs and 150 primary studies fulfilled the inclusion criteria, 91 from SRs, and 59 from our search strategy. Overall, the quality of the SRs was graded as "Critically low," and only two SRs were rated as "High" quality. 11 PICO questions were extracted from SRs and 31 from primary studies, which focused on osteosynthesis methods, surgical cutting devices, use of antibiotics, and induced hypotension. In addition, evidence bubble maps for each outcome were created to analyze in a visual manner the existing evidence. CONCLUSIONS: Future primary and secondary high-quality research should be addressed focused on the eight knowledge gaps identified in this mapping review. We concluded that the evidence mapping approach is a practical methodology for organizing the current evidence and identifying knowledge gaps in OS, helping to reduce research waste and canalize future efforts in developing studies for unsolved questions.


Subject(s)
Orthognathic Surgery , Humans , Systematic Reviews as Topic
2.
Med Oral Patol Oral Cir Bucal ; 26(6): e703-e710, 2021 Nov 01.
Article in English | MEDLINE | ID: mdl-34704984

ABSTRACT

BACKGROUND: The objective of this study was to determine the effect of antibiotic prophylaxis in preventing postoperative infections after extraction of impacted mandibular third molars. MATERIAL AND METHODS: A Parallel-group, randomized, blind, placebo-controlled trial was performed. 154 patients were randomly allocated to 2 groups; experimental (n=77) receiving 2g amoxicillin 1 hour prior to surgery and control (n=77) receiving placebo. Primary outcome was postoperative infections and secondary outcome was the need for rescue analgesia. RESULTS: 4.5% of patients developed postoperative infections, five patients of the control group (4 alveolar osteitis, 1 surgical site infection) and two of the experimental group (1 alveolar osteitis, 1 surgical site infection). Difference between groups was not statistically significant, RR=0.4 (95%CI 0.08-1.99, 𝘱=0.41) NNTB=26. Rescue analgesia intake was significantly higher in the control group (41 vs 18 patients of experimental group) RR=0.49 (95%CI 0.32-0.75, 𝘱<0.05) NNTB=3. CONCLUSIONS: The use of 2g amoxicillin 1 hour before surgery was not effective in significantly reducing the risk of postoperative infections from impacted mandibular third molars extraction, when compared to placebo. Nevertheless, antibiotic prophylaxis was associated with a reduced need for rescue analgesia.


Subject(s)
Dry Socket , Tooth, Impacted , Antibiotic Prophylaxis , Double-Blind Method , Dry Socket/etiology , Dry Socket/prevention & control , Humans , Molar, Third/surgery , Pain, Postoperative/etiology , Pain, Postoperative/prevention & control , Tooth Extraction , Tooth, Impacted/surgery
3.
HIV Med ; 22(4): 254-261, 2021 04.
Article in English | MEDLINE | ID: mdl-33336523

ABSTRACT

OBJECTIVES: To assess the effect of protease inhibitor (PI)-based dual therapy on CD4/CD8 ratio during the first year of therapy in antiretroviral therapy (ART)-naïve patients using data from randomized controlled clinical trials. METHODS: We pooled data from the GARDEL and ANDES studies, both randomized controlled clinical trials that recruited ART-naïve people living with HIV and randomly assigned them to receive PI-based dual therapy (DT) or triple therapy (TT) aiming to compare viral efficacy. We compared median CD4/CD8 ratios and the proportion of patients with CD4/CD8 ratio > 1 at 48 weeks after ART initiation in both treatment arms using the Mann-Whitney U-test and the χ2 test. We performed subgroup analysis for patients > 50 years old, with baseline CD4 counts ≤ 200 cells/µL, viral load > 100 000 HIV RNA copies/mL, and ritonavir-boosted lopinavir-based therapy. RESULTS: We analysed data from 571 patients: 292 on DT and 279 on TT. No differences were observed in CD4/CD8 ratio (0.632 vs. 0.617, P = 0.729) or in the proportion of patients with CD4/CD8 ratio > 1 (17.9% vs. 19.3%, P = 0.678) 48 weeks after ART initiation. Subgroup analysis showed no further differences. CONCLUSION: The impact of PI-based DT regimens on the CD4/CD8 ratio during the first year of treatment for ART-naïve patients is similar to that of TT.


Subject(s)
Anti-HIV Agents , HIV Infections , HIV Protease Inhibitors , HIV-1 , CD4 Lymphocyte Count , CD8-Positive T-Lymphocytes , HIV Infections/drug therapy , Humans , Lamivudine/therapeutic use , Middle Aged , Reverse Transcriptase Inhibitors , Ritonavir/pharmacology , Ritonavir/therapeutic use , Viral Load
4.
Actas Dermosifiliogr (Engl Ed) ; 111(5): 390-397, 2020 Jun.
Article in English, Spanish | MEDLINE | ID: mdl-32408973

ABSTRACT

BACKGROUND AND OBJECTIVE: The minimal erythema dose (MED), an essential measurement in studies of skin photosensitivity, requires establishing MED values for specific populations, given genetic variation. Different ways to assess erythema are also relevant. We aimed to determine MED values in a sample of Colombian patients and correlations between MED and Fitzpatrick skin type. We also studied concordance correlation between MEDs and two alternative ways to assess erythema. PATIENTS AND METHODS: Cross-sectional study of 113 individuals in Bogotá, Colombia. We used a solar simulator to measure UV-A radiation and combined UV-A and UV-B (UVA+UVB) radiation, o se podría suprimir este término porque UVA y UVB son términos conocidos for MED calculation. Narrowband UV-B (NBUVB) radiation was measured in a phototherapy cabin. Erythema was assessed visually and with a Mexameter MX 18 device. RESULTS: The median MEDs of UVA+UVB radiation were 22mJ/cm2 for Fitzpatrick skin typesI andII, and 33 and 43mJ/cm2, respectively, for typesIII andIV. The MEDs of UV-A radiation were 22, 42, 86, and 100J/cm2 for typesI, II, III, andIV, respectively. The MEDs of NBUVB light were 390, 550, 770, and 885mJ/cm2 for the 4 skin types. The correlation between MEDs and skin types ranged from 0.5 to 0.69. Lin's concordance correlation coefficients between visual and Mexameter assessments of erythema were greater than 0.8 in all cases. CONCLUSION: This study allowed us to understand MED values for UV-A, UVA+UVB, and NBUVB according to different skin types in the Colombian population. Concordance correlation coefficients between the different methods of erythema assessment were very good. Correlations between MEDs and skin types were moderate to good.


Subject(s)
Erythema , Skin Pigmentation , Colombia , Cross-Sectional Studies , Humans , Ultraviolet Rays/adverse effects
6.
Acta Ortop Mex ; 32(3): 140-144, 2018.
Article in Spanish | MEDLINE | ID: mdl-30521705

ABSTRACT

BACKGROUND: Fractures of the lateral third of the clavicle represent 18% of all clavicle fractures. Even though non-displaced fractures can be managed conservatively, displaced fractures have a non-union percentage of 30%. Multiple approaches have been proposed to manage the displaced fractures. The objective of this study was to record the prevalence of fractures of the lateral third of the clavicle with involvement of the clavicular-acromial joint, as well as to briefly review the treatment performed in our center and compare it with the current literature. MATERIAL AND METHODS: Retrospective, cross-sectional, descriptive and observational study, which consisted in a review of the records of patients with a diagnosis of fracture of the lateral third of the clavicle with an injury to the clavicular-acromial joint. RESULTS: Lateral third fractures accounted for 24% of all clavicle fractures. Distribution by sex: 10: 2, men: women. Left shoulder predominance, 8:4. The predominant maneuvers were anatomical plate for the lateral third of the clavicle (50% of the cases), coracoclavicular cerclage technique plus resection of the lateral third of the clavicle (25%), plate hook (8.3%), Mumford combined with Waever/Dunn (8.3%), anchor plus Kirschner rods (8.3%). DISCUSSION: The choice of the appropriate treatment will depend on the type of fracture and specific characteristics of each patient. We suggest the use of the coracoclavicular cerclage technique in cases in which the lateral fragment is multifragmented, irreducible or smaller in size than its osteosynthesis would allow, performing plasty of clavicular and coracoclavicular ligaments.


ANTECEDENTES: Las fracturas del tercio lateral de la clavícula representan 18% de todas las fracturas de clavícula. A pesar de que las fracturas no desplazadas pueden manejarse conservadoramente, las desplazadas tienen un porcentaje de no unión de 30%. Múltiples manejos han sido propuestos para las fracturas desplazadas. El objetivo de este estudio fue registrar la prevalencia de fracturas del tercio lateral de la clavícula con afectación de la articulación acromioclavicular, así como hacer una revisión breve del tratamiento realizado en nuestro centro y compararlo con la literatura actual. MATERIAL Y MÉTODOS: Estudio retrospectivo, transversal, descriptivo y observacional, que consistió en una revisión de los expedientes de los pacientes con diagnóstico de fractura del tercio lateral de la clavícula con lesión de la articulación acromioclavicular. RESULTADOS: Las fracturas del tercio lateral representaron 24% de todas las fracturas de clavícula. Distribución por sexos: 10:2, hombres:mujeres. Predominio: hombro izquierdo, 8:4. Los manejos predominantes fueron placa anatómica para tercio lateral de la clavícula (50% de los casos), técnica de cerclaje coracoclavicular más resección del tercio lateral de la clavícula (25%), placa gancho (8.3%), Mumford combinado con Waever/Dunn (8.3%), ancla más varillas Kirschner (8.3%). DISCUSIÓN: La elección del tratamiento adecuado dependerá del tipo de fractura y las características específicas de cada paciente. Sugerimos el uso de la técnica de cerclaje coracoclavicular en los casos en que el fragmento lateral sea multifragmentado, irreductible o de tamaño menor al que permitiría su osteosíntesis, realizando plastía de ligamentos acromioclaviculares y coracoclaviculares.


Subject(s)
Clavicle , Fractures, Bone , Bone Plates , Clavicle/injuries , Cross-Sectional Studies , Female , Fracture Fixation, Internal , Fractures, Bone/surgery , Humans , Male , Retrospective Studies , Treatment Outcome
7.
Acta ortop. mex ; 32(3): 140-144, may.-jun. 2018. graf
Article in Spanish | LILACS | ID: biblio-1054771

ABSTRACT

Resumen: Antecedentes: Las fracturas del tercio lateral de la clavícula representan 18% de todas las fracturas de clavícula. A pesar de que las fracturas no desplazadas pueden manejarse conservadoramente, las desplazadas tienen un porcentaje de no unión de 30%. Múltiples manejos han sido propuestos para las fracturas desplazadas. El objetivo de este estudio fue registrar la prevalencia de fracturas del tercio lateral de la clavícula con afectación de la articulación acromioclavicular, así como hacer una revisión breve del tratamiento realizado en nuestro centro y compararlo con la literatura actual. Material y métodos: Estudio retrospectivo, transversal, descriptivo y observacional, que consistió en una revisión de los expedientes de los pacientes con diagnóstico de fractura del tercio lateral de la clavícula con lesión de la articulación acromioclavicular. Resultados: Las fracturas del tercio lateral representaron 24% de todas las fracturas de clavícula. Distribución por sexos: 10:2, hombres:mujeres. Predominio: hombro izquierdo, 8:4. Los manejos predominantes fueron placa anatómica para tercio lateral de la clavícula (50% de los casos), técnica de cerclaje coracoclavicular más resección del tercio lateral de la clavícula (25%), placa gancho (8.3%), Mumford combinado con Waever/Dunn (8.3%), ancla más varillas Kirschner (8.3%). Discusión: La elección del tratamiento adecuado dependerá del tipo de fractura y las características específicas de cada paciente. Sugerimos el uso de la técnica de cerclaje coracoclavicular en los casos en que el fragmento lateral sea multifragmentado, irreductible o de tamaño menor al que permitiría su osteosíntesis, realizando plastía de ligamentos acromioclaviculares y coracoclaviculares.


Abstract: Background: Fractures of the lateral third of the clavicle represent 18% of all clavicle fractures. Even though non-displaced fractures can be managed conservatively, displaced fractures have a non-union percentage of 30%. Multiple approaches have been proposed to manage the displaced fractures. The objective of this study was to record the prevalence of fractures of the lateral third of the clavicle with involvement of the clavicular-acromial joint, as well as to briefly review the treatment performed in our center and compare it with the current literature. Material and methods: Retrospective, cross-sectional, descriptive and observational study, which consisted in a review of the records of patients with a diagnosis of fracture of the lateral third of the clavicle with an injury to the clavicular-acromial joint. Results: Lateral third fractures accounted for 24% of all clavicle fractures. Distribution by sex: 10: 2, men: women. Left shoulder predominance, 8:4. The predominant maneuvers were anatomical plate for the lateral third of the clavicle (50% of the cases), coracoclavicular cerclage technique plus resection of the lateral third of the clavicle (25%), plate hook (8.3%), Mumford combined with Waever/Dunn (8.3%), anchor plus Kirschner rods (8.3%). Discussion: The choice of the appropriate treatment will depend on the type of fracture and specific characteristics of each patient. We suggest the use of the coracoclavicular cerclage technique in cases in which the lateral fragment is multifragmented, irreducible or smaller in size than its osteosynthesis would allow, performing plasty of clavicular and coracoclavicular ligaments.


Subject(s)
Humans , Male , Female , Clavicle/injuries , Fractures, Bone/surgery , Bone Plates , Cross-Sectional Studies , Retrospective Studies , Treatment Outcome , Fracture Fixation, Internal
8.
Medwave ; 18(7): e7344, 2018.
Article in English, Spanish | LILACS | ID: biblio-966468

ABSTRACT

INTRODUCCIÓN: Las fracturas maxilofaciales se asocian a importante morbilidad, pérdida de función y secuelas estéticas, entre otros. Dentro de las fracturas mandibulares, las fracturas de cóndilo mandibular son las más frecuentes. Estas pueden ser tratadas mediante un tratamiento quirúrgico (reducción abierta más estabilización con miniplacas de titanio) o un tratamiento conservador (ortopédico). MÉTODOS: Realizamos una búsqueda en Epistemonikos, la mayor base de datos de revisiones sistemáticas en salud, la cual es mantenida mediante el cribado de múltiples fuentes de información, incluyendo MEDLINE, EMBASE, Cochrane, entre otras. Extrajimos los datos desde las revisiones identificadas, analizamos los datos de los estudios primarios, realizamos un metanálisis y preparamos una tabla de resumen de los resultados utilizando el método GRADE. RESULTADOS Y CONCLUSIONES: Identificamos ocho revisiones sistemáticas que en conjunto incluyen 66 estudios primarios, de los cuales, seis corresponden a ensayos aleatorizados. Concluimos que, en comparación con el tratamiento conservador, el tratamiento quirúrgico en fracturas de cóndilo mandibular probablemente se asocia a menor dolor articular, menor maloclusión y menor desviación lateral en apertura bucal.


INTRODUCTION: Maxillofacial fractures are associated with significant morbidity, loss of function and aesthetic sequelae, among others. Within mandibular fractures, mandibular condylar fractures are the most frequent. These can be treated by surgical treatment or conservative treatment (orthopedic). METHODS: We searched in Epistemonikos, the largest database of systematic reviews in health, which is maintained by screening multiple information sources, including MEDLINE, EMBASE, Cochrane, among others. We extracted data from the systematic reviews, reanalyzed data of primary studies, conducted a meta-analysis and generated a summary of findings table using the GRADE approach. RESULTS AND CONCLUSIONS: We identified eight systematic reviews including 66 primary studies overall, of which six were randomized trials. We concluded surgical treatment of mandibular condyle fractures, compared to conservative treatment, is probably associated with less joint pain, less malocclusion and less lateral deviation in buccal opening.


Subject(s)
Humans , Orthopedic Procedures/methods , Mandibular Condyle/injuries , Mandibular Fractures/therapy , Randomized Controlled Trials as Topic , Databases, Factual , Conservative Treatment/methods , Mandibular Fractures/pathology
10.
Acta ortop. mex ; 29(4): 203-206, jul.-ago. 2015. ilus
Article in Spanish | LILACS | ID: lil-781218

ABSTRACT

Introducción: El manejo ideal de las lesiones tipo III en agudo es controversial, dentro del manejo quirúrgico de estas lesiones es fundamental la reconstrucción de los ligamentos coracoclaviculares. Material y métodos: Se revisaron los expedientes de los pacientes con luxación acromioclavicular aguda, tratadas mediante reducción abierta y fijación con suturas de alta resistencia evaluando los resultados de manera preliminar a tres meses de evolución. Resultados: Fueron 12 pacientes; todos del sexo masculino, con una edad media de 28 años; tres de grado III, uno grado IV y ocho grado V de la clasificación de Rockwood. El tiempo promedio entre la lesión y la intervención quirúrgica fue de nueve días. En la escala de Constant 11 pacientes presentaron resultados excelentes y 1 bueno. Sólo un paciente desarrolló un seroma. La reducción radiográfica se mantuvo en todos los pacientes. Conclusiones: La reconstrucción anatómica de los ligamentos coracoclaviculares disminuye notablemente las complicaciones, más aún cuando dicha reconstrucción es provista por materiales que tengan una fuerza tensil semejante a los ligamentos nativos.


Introduction: Determining what is the ideal acute management of type III lesions is controversial. The reconstruction of coracoclavicular ligaments is a fundamental part of the surgical management of these lesions. Material and methods: The records of patients with acute acromioclavicular dislocation treated with open reduction and fixation with highly resistant sutures were reviewed. The preliminary results were assessed at the 3-month follow-up. Results: Twelve patients were included, all of them males; mean age was 28 years. Three were Rockwood grade III, one grade IV, and 8 grade V. The mean time elapsed between the injury and the surgery was 9 days. According to the Constant scale 11 patients had excellent results and one had good results. Only one patient developed a seroma. Radiographic reduction was maintained in all patients. Conclusions: The anatomical reconstruction of coracoclavicular ligaments reduces complications considerably, especially when such reconstruction is made using materials with a grip strength similar to the one offered by native ligaments.

11.
Acta Ortop Mex ; 29(4): 203-6, 2015.
Article in Spanish | MEDLINE | ID: mdl-27186996

ABSTRACT

INTRODUCTION: Determining what is the ideal acute management of type III lesions is controversial. The reconstruction of coracoclavicular ligaments is a fundamental part of the surgical management of these lesions. MATERIAL AND METHODS: The records of patients with acute acromioclavicular dislocation treated with open reduction and fixation with highly resistant sutures were reviewed. The preliminary results were assessed at the 3-month follow-up. RESULTS: Twelve patients were included, all of them males; mean age was 28 years. Three were Rockwood grade III, one grade IV, and 8 grade V. The mean time elapsed between the injury and the surgery was 9 days. According to the Constant scale 11 patients had excellent results and one had good results. Only one patient developed a seroma. Radiographic reduction was maintained in all patients. CONCLUSIONS: The anatomical reconstruction of coracoclavicular ligaments reduces complications considerably, especially when such reconstruction is made using materials with a grip strength similar to the one offered by native ligaments.


INTRODUCCIÓN: El manejo ideal de las lesiones tipo III en agudo es controversial, dentro del manejo quirúrgico de estas lesiones es fundamental la reconstrucción de los ligamentos coracoclaviculares. MATERIAL Y MÉTODOS: Se revisaron los expedientes de los pacientes con luxación acromioclavicular aguda, tratadas mediante reducción abierta y fijación con suturas de alta resistencia evaluando los resultados de manera preliminar a tres meses de evolución. RESULTADOS: Fueron 12 pacientes; todos del sexo masculino, con una edad media de 28 años; tres de grado III, uno grado IV y ocho grado V de la clasificación de Rockwood. El tiempo promedio entre la lesión y la intervención quirúrgica fue de nueve días. En la escala de Constant 11 pacientes presentaron resultados excelentes y 1 bueno. Sólo un paciente desarrolló un seroma. La reducción radiográfica se mantuvo en todos los pacientes. CONCLUSIONES: La reconstrucción anatómica de los ligamentos coracoclaviculares disminuye notablemente las complicaciones, más aún cuando dicha reconstrucción es provista por materiales que tengan una fuerza tensil semejante a los ligamentos nativos.

14.
J Cell Biochem ; 80(2): 274-84, 2000 Oct 20.
Article in English | MEDLINE | ID: mdl-11074599

ABSTRACT

Morphologic studies have shown that the classic endocytosis tracer horseradish peroxidase (HRP) is actively internalized by vesicular transport in the carp intestine, suggesting the existence of specific binding sites in the apical membrane of enterocytes. The aim of the present study was to develop an in vitro binding assay using isolated carp intestinal brush-border membranes (BBM) to demonstrate and characterize these specific HRP binding sites. The results obtained show that HRP binding to BBM exhibits a saturable mode and high affinity (K(d) = 22 nM). In addition, HRP binding sites are highly enriched in BBM compared to basolateral membranes. On the other hand, HRP interaction with these sites is apparently of an ionic character because binding increased concomitantly with decreasing NaCl concentrations in the assay, reaching a maximum in the absence of NaCl. Other proteins that are also internalized in carp intestine did not significantly inhibit HRP binding to BBM. A lectin-type of interaction was discarded because neither manan nor ovoalbumin inhibited HRP binding. Proteinase K treatment of BBM reduced HRP binding by 70%, suggesting a proteic nature for this binding site. Finally, ligand blotting assays showed that HRP binds specifically to a 15.3-kDa protein. Taken together, these results are consistent with the existence of a functional receptor for HRP in carp intestinal mucosa that could mediate its internalization.


Subject(s)
Horseradish Peroxidase/metabolism , Intestinal Mucosa/metabolism , Microvilli/metabolism , Animals , Blotting, Western , Carps , Cell Membrane/metabolism , Electrophoresis, Polyacrylamide Gel , Protein Binding
15.
Int J Occup Environ Health ; 6(3): 177-82, 2000.
Article in English | MEDLINE | ID: mdl-10926720

ABSTRACT

To investigate whether the agricultural use of untreated wastewater (i.e. crop irrigation) was associated with elevated blood lead levels in a farming population in the Mezquital Valley and which risk factors, other than exposure to untreated wastewater, were associated with elevated blood lead levels, lead levels were measured in venous blood obtained from 735 individuals. Blood samples were analyzed by atomic absorption spectrophotometry. Food habits and dietary intake were gathered by interview, using a semi-quantitative food-frequency questionnaire. The average blood lead level was 7.8 microg/dL (SD 4.66 microg/dL; range 1.2-36.7 microg/dL). 23% of the study population had blood lead levels exceeding 10 microg/dL. The use of lead-glazed ceramics (LGC) was significantly associated with elevated lead levels (p = < 0.001). Other significant variables included age, gender (males), and non-farming-related occupations (e.g., technicians, factory workers). p = 0.005, 0.08, and 0.001, respectively. When the analysis was stratified by the use of LGC for food preparation, an inverse relationship between higher daily calcium intake and blood lead level was detected (beta = - 0.040, p = < 0.05). Thus, blood lead levels were positively associated with the use of LGC. Calcium intake showed a protective effect, maybe by decreasing absorption of lead in the gastrointestinal tract. No association between occupational exposure to untreated wastewater or crop consumption and blood lead levels was detected. Further environmental and health surveillance is recommended.


Subject(s)
Agricultural Workers' Diseases/blood , Agricultural Workers' Diseases/etiology , Agriculture , Lead Poisoning/blood , Lead Poisoning/etiology , Lead/blood , Occupational Exposure/adverse effects , Occupational Exposure/analysis , Waste Disposal, Fluid , Adolescent , Adult , Calcium, Dietary/analysis , Ceramics , Feeding Behavior , Female , Humans , Intestinal Absorption , Male , Mexico , Predictive Value of Tests , Risk Factors , Surveys and Questionnaires
16.
Arch Med Res ; 31(6): 551-7, 2000.
Article in English | MEDLINE | ID: mdl-11257320

ABSTRACT

BACKGROUND: Because of their lack of long-term viability, adrenal tissue transplants have shown limited success in alleviating the motor disturbances associated with experimental and pathologic striatal dopamine denervation. In this study, we examined how the graft placement site influences adrenal medulla transplant survival and its relation with the reduction of motor deficits in rats bearing unilateral 6-OHDA lesion. METHODS: One or 5 microL of fetal adrenal medullar tissue was grafted either inside the striatal parenchyma or into the lateral ventricle in contact with the dopamine-denervated striatum. Motor disturbances, as assessed by apomorphine-induced rotation, were correlated to the graft morphologic survival features. RESULTS: Apomorphine-induced rotation showed a marginal reduction of 11% in all groups independently of graft survival features or placement site. Intrastriatal transplants showed limited viability characterized by a substantial loss of graft initial volume as well as fewer and smaller chromaffin cells compared to ventricular grafts, which had a reduced loss of graft initial volume and more and larger chromaffin cells. CONCLUSIONS: Although the lateral ventricle may favor adrenal medulla transplant viability, their induced motor outcome is comparable to that induced by less viable intrastriatal grafts, suggesting that the implanted dopamine-producing cells may interact and influence striatal neurons better when placed in close proximity.


Subject(s)
Adrenal Medulla/transplantation , Cerebral Ventricles/surgery , Corpus Striatum/surgery , Fetal Tissue Transplantation , Motor Activity , Transplantation, Heterotopic , Adrenal Medulla/embryology , Adrenal Medulla/metabolism , Animals , Apomorphine , Biomarkers , Cell Size , Chromaffin Cells/enzymology , Chromaffin Cells/pathology , Corpus Striatum/drug effects , Corpus Striatum/physiopathology , Dopamine/metabolism , Graft Survival , Male , Motor Activity/drug effects , Nerve Tissue Proteins/analysis , Neurotoxins/toxicity , Oxidopamine/toxicity , Parkinson Disease, Secondary/chemically induced , Parkinson Disease, Secondary/surgery , Rats , Rats, Wistar , Species Specificity , Stereotaxic Techniques , Sympathectomy, Chemical , Tyrosine 3-Monooxygenase/analysis
17.
Neurosci Lett ; 274(1): 1-4, 1999 Oct 15.
Article in English | MEDLINE | ID: mdl-10530505

ABSTRACT

The interaction of ozone with some molecules results in an increased production of free radicals. The objective of this study was to identify whether acute ozone exposure to 1-1.5 ppm for 4 h, produced cytological and ultrastructural modifications in the olfactory bulb cells. The results showed that in rats exposed to ozone there was a significant loss of dendritic spines on primary and secondary dendrites of granule cells, whereas the control rats did not present such changes. Besides these exposed cells showed vacuolation of neuronal cytoplasm, swelling of Golgi apparatus and mitochondrion, dilation cisterns of the rough endoplasmic reticulum. These findings suggest that oxidative stress produced by ozone induces alterations in the granule layer of the olfactory bulb, which may be related to functional modifications.


Subject(s)
Dendrites/drug effects , Olfactory Bulb/drug effects , Ozone/toxicity , Animals , Dendrites/ultrastructure , Free Radicals , Inhalation Exposure , Male , Microscopy, Electron , Olfactory Bulb/ultrastructure , Organelles/drug effects , Organelles/ultrastructure , Ozone/administration & dosage , Rats , Rats, Wistar
18.
Neurosci Lett ; 270(2): 107-9, 1999 Jul 30.
Article in English | MEDLINE | ID: mdl-10462109

ABSTRACT

Exposure to ozone results in an increased production of free radicals which causes oxidative stress. The purpose of this study was to determine the effects of ozone exposure on memory and its correlation with the cytology of the hippocampus. Twenty-four male Wistar rats were exposed to 1 ppm (parts per million) ozone for 4 h in a closed chamber. Control group was exposed to flowing air. After ozone exposure, the rats were given long-term (24 h) memory training which consists of a passive avoidance conditioning. After that the animals were perfused and the brains were placed in the Golgi stain. The analysis consisted in counting the dendritic spines in five secondary and five tertiary dendrites of each of the 20 pyramidal neurons of hippocampus CA1 analyzed. Our results showed alterations on long-term memory and a significant reduction of dendritic spines, and provided evidence that this deterioration in memory is probably due to the reduction in spine density in the pyramidal neurons of hippocampus.


Subject(s)
Hippocampus/pathology , Memory , Oxidative Stress/physiology , Animals , Avoidance Learning/physiology , Dendrites/ultrastructure , Hippocampus/drug effects , Hippocampus/ultrastructure , Male , Memory/drug effects , Ozone/pharmacology , Pyramidal Cells/ultrastructure , Rats , Rats, Wistar
19.
Rev Stomatol Chir Maxillofac ; 97(5): 258-63, 1996 Oct.
Article in French | MEDLINE | ID: mdl-8984588

ABSTRACT

The objective of this research is to compare the ultrasonographic (USG) and sialographic contributions for the diagnosis of Recurrent Parotitis in Childhood (RPC). Bilateral USG of the parotid gland was performed among 16 children with RPC and 16 healthy children. The image of the normal parotid glands was compared with the image of the parotid gland with RPC. A pathologic USG pattern for RPC was obtained which consisted in a homogeneous, micronodular parenchyme with a diffuse increase density, sometimes with multiples hypoechogenous areas, that were considered solid hypoechogenous nodules suggesting inflammatory nodes. A glandular contour finely granulated was described with normal peri-parotidareas and with aponeurotic-muscular planes. Then 16 USG of the parotid gland of the children with RPC were compared with 16 Sialography previous obtained. The possibilities of diagnosis of the disease with both techniques was not different (p > 0.05). When the clinic was correlated with the USG it was possible to obtain parameters of severity of the glandular damage and to visualise the degree of changes of the parenchyma of each child with RPC. In some cases, bilateral damage was observed in children with clinical unilateral symptoms. The USG was performed in children in the acute phase of disease, visualising the microabscess in the gland and confirming the previous diagnosis. Finally, it was possible to conclude the utility of USG in the study of the disease. Being a not invasive exam, harmless, unpainfully echography, was easily accepted by the child. Also, we obtain information not only about the diagnosis of the disease, but also about the severity, prognosis, treatment and follow-up of the infant patients.


Subject(s)
Parotitis/diagnostic imaging , Sialography , Abscess/diagnostic imaging , Acute Disease , Child , Child, Preschool , Chronic Disease , Fascia/diagnostic imaging , Female , Follow-Up Studies , Humans , Male , Neck Muscles/diagnostic imaging , Parotid Gland/anatomy & histology , Parotid Gland/diagnostic imaging , Parotitis/pathology , Parotitis/therapy , Prognosis , Recurrence , Ultrasonography
SELECTION OF CITATIONS
SEARCH DETAIL