Association between Zika virus and fetopathy: a prospective cohort study in French Guiana.

OBJECTIVE: To establish the incidence of fetal central nervous system (CNS) anomalies (including microcephaly), signs of congenital infection and fetal loss in pregnant women infected with Zika virus (ZIKV) and non-infected pregnant women in western French Guiana. METHODS: This prospective cohort study was conducted between 1 January and 15 July 2016. We evaluated and compared clinical and fetal ultrasound examinations of 301 pregnant women with biological confirmation of ZIKV infection and 399 pregnant women who were negative for ZIKV infection. RESULTS: Overall, the total number of fetuses with CNS involvement was higher in the infected than in the control group (9.0% vs 4.3%; relative risk, 2.11 (95% CI, 1.18-4.13)). Anomalies of the corpus callosum and presence of cerebral hyperechogenicities were significantly more common in the infected group. There was an increased risk of microcephaly in the infected compared with the control group (1.7% vs 0.3%; relative risk, 6.63 (95% CI, 0.78-57.83)), although this was not statistically significant. When the mother was infected during the first or second trimester, there was a greater risk of severe CNS involvement, more signs of infection and intrauterine fetal death than with infection in the third trimester. The rate of vertical transmission in the exposed group was 10.9%. CONCLUSION: ZIKV infection during pregnancy is associated with a significant risk of fetal CNS involvement and intrauterine fetal death, particularly when infection occurs during the first or second trimesters. Microcephaly was not present in every case of congenital ZIKV syndrome that we observed. Until more is known about this disease, it is paramount to evaluate suspected cases by detailed neurosonography on a monthly basis, paying particular attention to the corpus callosum and the presence of hyperechogenic foci. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.

Three-dimensional ultrasound fetal urinary bladder volume measurement: reliability of rotational (VOCAL) technique using different steps of rotation.

OBJECTIVE: The aim of the study was to investigate the inter- and intraobserver reliability in measuring the fetal bladder volume by 3d ultrasound imaging by VOCAL. In addition a comparison of 15 degrees and 30 degrees rotation steps calculation had been performed. METHODS: Measurements (n = 240) of FB volume were performed in 30 low-risk patients with gestation times ranging from 22 to 39 weeks by two independent observers blind to each other. All fetuses were examined and no ultrasound detectable malformation was found. The volume for each FB was measured twice by the observers who used the Virtual Organ Computed-aided Analysis (VOCAL) technique. Distinct sets of 12 and 6 planes were obtained after sequential rotations of 15 degrees and 30 degrees, respectively. The internal contour of fetal bladder was determined manually and carefully as to exclude adjacent structures from volume calculation. Wilcoxon signed-rank test was used for the comparison of paired samples in the cases of replication within and between observers. Spearman's rank correlation was used to study the relationship among angles. Bland and Altman's graphical approach was used to investigate the agreement between observers. RESULTS: Both techniques were shown to be highly reliable. No significant difference was found between the measurements of FB volume with the VOCAL technique by varying either the steps of rotation or the observers. CONCLUSION: Excellent correlations were found for both observers in the use of rotation angles of 15 degrees and 30 degrees . Since a significantly faster evaluation was obtained by using a 30 degrees rotation step it must be preferred to assess the FB volume. Our results show that the VOCAL technique, with a plane rotation of 30 degrees , can be used in a simple way to estimate fetal urine production.