Peculiarities of the free radical processes in rat liver mitochondria under toxic hepatitis on the background of alimentary protein deficiency.

The rate of superoxide anion radical, hydroxyl radical and hydrogen peroxide generation, the level of oxidative modification of mitochondrial proteins in the liver of rats with toxic hepatitis was investigated on the background of alimentary protein deficiency. We did not find significant increases of the intensity of free radical processes in liver mitochondria of rats maintained on the protein-deficient ration. The most significant intensification of free radical processes in liver mitochondria is observed under the conditions of toxic hepatitis, induced on the background of alimentary protein deprivation. Under these conditions the aggravation of all studied forms of reactive oxygen species generation was observed in liver mitochondria. The generation rates were increased as follows: O2 ­ by 1.7 times, Н2О2 ­ by 1.5 times, •ОН ­ practically double on the background of accumulation of oxidized mitochondria-derived proteins. The established changes in thiol groups' redox status of respiratory chain proteins insoluble in 0.05 M sodium-phosphate buffer (pH 11.5), and changes of their carbonyl derivatives content may be considered as one of the regulatory factors of mitochondrial energy-generating function.

[NADH:ubiquinone reductase and succinate dehydrogenase activity in the liver of rats with acetaminophen-induced toxic hepatitis on the background of alimentary protein deficiency].

The ratio between the redox forms of the nicotinamide coenzymes and key enzymatic activity of the I and II respiratory chain complexes in the liver cells mitochondria of rats with acetaminophen-induced hepatitis under the conditions of alimentary deprivation of protein was studied. It was estimated, that under the conditions of acute acetaminophen-induced hepatitis of rats kept on a low-protein diet during 4 weeks a significant decrease of the NADH:ubiquinone reductase and succinate dehydrogenase activity with simultaneous increase of the ratio between redox forms of the nicotinamide coenzymes (NAD+/NADH) is observed compared to the same indices in the liver cells of animals with experimental hepatitis kept on the ration balanced by all nutrients. Results of research may become basic ones for the biochemical rationale for the approaches directed to the correction and elimination of the consequences of energy exchange in the toxic hepatitis, induced on the background of protein deficiency.

[Effect of low doses of ionizing radiaton on the fractional content of mitochondrial proteins and mtDNA in Guerin's carcinoma].

The work is devoted to investigation of the influence of low doses of preliminary radiation on the fraction content, and oxidizing modification of mitochondrial proteins, unsolvable in 0.05 M sodium-phosphate buffer (11,5), and mtDNA fragmentation. That low-dose irradiation influence mostly manifests itself on the initial stages of tumor growth. It was established that tumor development is accompanied with the changes of mitochondrial proteins fraction content. These changes occured on the intensive oxidizing modification background of the proteins under study and mtDNA fragmentation.

Oxidative destruction of mitochondrial translation products after fractionated irradiation of rats with tumor.

We demonstrated specific features of oxidative damage to mitochondrial translation products in animals were revealed after fractionated X-ray exposure in low doses. Differences were found in carbonylation and oxidation of SH groups in irradiated rats and irradiated tumor-bearing animals. Our results indicate that preliminary fractionated X-ray exposure determines oxidative damage to these mitochondrial proteins only at the initial stage of the study, while in the follow-up period major role is played the by tumor development.

[A commercial immunoenzyme reagent for the determination of ceruloplasmin]. / Kommercheskii immunofermentnyi diagnostikum dlia opredeleniia tseruloplazmina.

A diagnostic agent for enzyme immunoassay of ceruloplasmin has been developed. The method is highly sensitive and specific. The minimal detectable amount of ceruloplasmin is 25 ng/ml, variance coefficients with 1 lot being 2.6-7.3 percent and 4.2-10.8 percent within 3 lots. Blood serum ceruloplasmin concentration in health has made up 0.9 to 1.3 mg/ml. Ceruloplasmin levels were regularly shifted in Wilson's disease, cerebral lymphomas, sepsis, injuries, liver cirrhosis and other diseases.

[Plasma ceruloplasmin in patients with hepatorenal insufficiency]. / Plazmennyi tseruloplazmin u bol'nykh s pechenochno-pochechnoi nedostatochnost'iu.

Changes in plasma ceruloplasmin levels were studied by enzyme immunoassay in 81 patients with the acute hepatorenal insufficiency syndrome (AHIS). Changed plasma ceruloplasmin concentration was detected in the first days of the disease, this change depending on AHIS severity and unrelated to the disease cause. A lowering of plasma ceruloplasmin level below 0.75 mg/ml is considered to be a poor prognostic sign. Measurement of plasma ceruloplasmin is a test available for clinical laboratories, it is highly sensitive, accurate, and reproducible.

[Comparative characterization of the polystyrene plates used in enzyme immunoassay]. / Sravnitel'naia kharakteristika polistirolovykh planshetov, primeniaemykh v IFA.

The data on the comparison of the quality of polystyrene plates manufactured in the USSR with those manufactured by a number of foreign producers are presented. These data indicate that the capacity of antibodies adsorbed on a polystyrene plate for the sorption of antigens depend on the properties of the polymer surface and vary for different plates.

[Possibilities of using immunoenzyme test systems for determining levels of myoglobin, fibrinogen, fibrin-fibrinogen degradation products and fibronectin in the diagnosis of somatic diseases]. / Vozmozhnosti primeneniia immunofermentnykh test-sistem dlia opredeleniia soderzhaniia mioglobina, fibrinogena i produktov degradatsii fibrin-fibrinogena, fibronektina v diagnostike somaticheskikh zabolevanii.

Clinical trials of immunoenzyme test-systems for the quantitative determination of fibronectin, fibrinogen, fibrin/fibrinogen degradation products and myoglobin have been performed on serum and plasma samples obtained from patients and healthy donors. The tests were informative and possessed diagnostic value in the following conditions: fibronectin--in pyogenic septic complications of newborns, burn infections; fibrinogen and fibrin/fibrinogen products--in thrombosis, myocardial infarction and disseminated intravascular coagulation syndrome; myoglobin--in myocardial infarction.

[Quantitative immunoenzyme determination of fibronectin in biological fluids]. / Kolichestvennoe immunofermentnoe opredelenie fibronektina v biologicheskikh zhidkostiakh.

Quantitative immunoenzyme assay of fibronectin in blood plasma is developed. Isolation and purification of fibronectin from human blood plasma, harvesting of specific antibodies to fibronectin, production of the antibodies and peroxidase conjugates and step-by-step procedure of the immunoenzyme assay in a "sandwich" modification are described. The assay was applied for diagnostics of several infectious and somatic diseases.