ABSTRACT
We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD markers; 156 reliable bands were found for both genotypes, of which 24% were polymorphic. Fifteen primers gave at least one consistent polymorphic band. The dendogram developed by pooling data of RAPD analysis of summer and winter genotypes had similar patterns. This technique allowed us to examine the relationship between canola genotypes.
Subject(s)
Brassica napus/genetics , DNA, Plant/genetics , Genotype , Polymorphism, Genetic , Seasons , Seeds/genetics , TurkeyABSTRACT
RT-qPCR was used to analyze the vitamin D receptor (VDR) gene TaqI polymorphism in 100 Turkish patients with type 2 diabetes mellitus (T2DM) and hypertension compared with 100 healthy subjects, to determine whether VDR could be considered as one of the susceptibility genes for T2DM and hypertension. Genotyping was done with PCR, followed by melting curve analysis with specific fluorescent hybridization probes. The results showed that distributions for TT, Tt and tt genotypes were 51, 46 and 3% in the patient group, and 35, 49 and 16% in the control group, respectively. The frequency of the T allele in patients was also significantly higher than that in controls. Based on the results, the relationship between the VDR gene TaqI polymorphism and T2DM patients in the Turkish population was compared. In terms of the genotype distributions and allele frequencies of the VDR gene TaqI polymorphism, there was no statistically significant difference (P > 0.05) between the T2DM and hypertension patients and controls. Application of RT-qPCR method enabled us to assess the prevalence of the VDR gene TaqI polymorphism and its association with type 2 diabetes and hypertension.