Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes.

This study investigated how metabolite analysis can explain differences in tissue composition and size in fish from different habitats. We, therefore, studied Nile tilapia (Oreochromis niloticus) from three Ethiopian lakes (Gilgel Gibe, Ziway, and Langano) using dried bloodspot (DBS) analysis of carnitine esters and free amino acids. A total of sixty (N = 60) Nile tilapia samples were collected comprising twenty (n = 20) fish from each lake. The proximate composition of the targeted tissues (muscle, skin, gill, gut, and liver) were analyzed. The DBS samples were analyzed for acylcarnitine and free amino acid profiles using quantitative electrospray tandem mass spectrometry. Metabolite ratios were calculated from relevant biochemical pathways that could identify relative changes in nutrient metabolism. The mean weight of Nile tilapia sampled from each lake showed weight variation among the lakes, fish from Lake Ziway were largest (178 g), followed by Gilgel Gibe reservoir (134 g) and Lake Langano (118 g). Fish from Gilgel Gibe showed significantly higher fat composition in all tissues (P < 0.05) except the liver in which no significant variation was observed. The source of fish affected the tissue fat composition. Marked differences were observed in Nile tilapia metabolic activity between the lakes. For instance, the lower body weight and condition of the fish in Lake Langano coincided with several metabolite ratios pointing to a low flow of glucogenic substrate to the citric acid cycle. The low propionyl to acetylcarnitine ratio (C3:C2) in Gilgel Gibe fish is indicating that more of the available acetyl CoA is not led into the citric acid cycle, but instead will be used for fat synthesis. The metabolic markers for lipogenesis and metabolic rate could explain the high-fat concentration in several parts of the body composition of fish from Gilgel Gibe. Our results show that nutrition-related blood metabolite ratios are useful to understand the underlying metabolic events leading to the habitat-dependent differences in the growth of Nile tilapia, and by extension, other species.

Diversity in Micromineral Distribution Within the Body of Ornamental Fish Species.

This study was conducted to compare micromineral homeostasis across ornamental fish species. Ten different species (n = 3, total = 30) of live ornamental fish were randomly sampled from one big aquarium in a pet store in Belgium. All fish samples were dissected manually for the collection of targeted tissues. The tissue samples were ashed by microwave oven, and the extract was analyzed for copper (Cu), iron (Fe), and Zinc (Zn) by inductively coupled plasma mass spectrometry. Fe was associated with Cu in muscle tissue (p < 0.05), but neither of them were associated with Zn in the muscle. However, the three micromineral concentrations were correlated in the heart (p < 0.05). Similarly, all of them were correlated in the liver (p < 0.05), but none of them showed a significant association in the tail fin. Excess deposition of minerals in heart tissue is a new observation, and it is not known if this is meant as storage or rather the fish heart has a high requirement for microminerals. Storage in the tail fin should be interpreted as a sign of permanent deposition as a tool to dispose off toxic excess. The lack of correlation between the muscular concentrations of Zn on the one hand, and those of Fe and Cu on the other hand, further suggests that fish species distinctly differ in their micromineral metabolism. Although this exploratory study still leaves many questions unanswered, it points to the large diversity in micromineral metabolism among fish species.